• Animal Bioscience
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• v.34 no.10
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• pp.1579-1589
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• 2021

Objective: This study was conducted to generate single stranded DNA oligonucleotides with selective affinity to bovine spermatozoa, assess its binding potential and explore its potential utility in trapping spermatozoa from suspensions. Methods: A combinatorial library of 94 mer long oligonucleotide was used for systematic evolution of ligands by exponential enrichment (SELEX) with bovine spermatozoa. The amplicons from sixth and seventh rounds of SELEX were sequenced, and the reads were clustered employing cluster database at high identity with tolerance (CD-HIT) and FASTAptamer. The enriched nucleotides were predicted for secondary structures by Mfold, motifs by Multiple Em for Motif Elicitation and 5' labelled with biotin/6-FAM to determine the binding potential and binding pattern. Results: We generated 14.1 and 17.7 million reads from sixth and seventh rounds of SELEX respectively to bovine spermatozoa. The CD-HIT clustered 78,098 and 21,196 reads in the top ten clusters and FASTAptamer identified 2,195 and 4,405 unique sequences in the top three clusters from the sixth and seventh rounds, respectively. The identified oligonucleotides formed secondary structures with delta G values between -1.17 to -26.18 kcal/mol indicating varied stability. Confocal imaging with the oligonucleotides from the seventh round revealed different patterns of binding to bovine spermatozoa (fluorescence of the whole head, spot of fluorescence in head and mid- piece and tail). Use of a 5'-biotin tagged oligonucleotide from the sixth round at 100 pmol with 4×10⁶ spermatozoa could trap almost 80% from the suspension. Conclusion: The binding patterns and ability of the identified oligonucleotides confirms successful optimization of the SELEX process and generation of aptamers to bovine spermatozoa. These oligonucleotides provide a quick approach for selective capture of spermatozoa from complex samples. Future SELEX rounds with X- or Y- enriched sperm suspension will be used to generate oligonucleotides that bind to spermatozoa of a specific sex type.

• Journal of the Korean Applied Science and Technology
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• v.41 no.3
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• pp.825-841
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• 2024

This study aimed to scrutinize the evolution of cosmetics research and development (R&D) in Korea, utilizing data from nationally funded R&D projects, and to suggest strategies to enhance the competitiveness of small and medium-sized enterprises (SMEs). A thorough analysis was performed on cosmetics-related national R&D funded project data from 2019 to 2023, a comprehensive analysis was conducted on annual trends, key organization, regional characteristics, major departments, and main functions and efficacy of R&D. The features revealed that the cosmetics industry, predominantly comprised of SMEs, is directing its efforts towards the development of a diverse range of functional products, with a recent shift towards the production of eco-friendly and sustainable materials. From a regional perspective, Gyeonggi-do and Chungcheongbuk-do had the highest levels of R&D activities, demonstrating the significant R&D capabilities of these local industry. It provides a systematic comprehensive analysis based on national R&D funded project data, identifies recent trends in the cosmetics industry, and evidence for SMEs to strengthen market competitiveness and establish long-term strategies for sustainable growth. Therefore, the results of this study dispense significant information and insights not only to SMEs but also to policymakers, using critical foundational data for formulating and implementing policies to advance the cosmetics industry.

• Economic and Environmental Geology
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• v.28 no.4
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• pp.337-350
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• 1995

The Hwacheon-ri mineralized area is located within the Cretaceous Gyeongsang Basin of the Korean peninsula. The mineralized area includes the Hwacheon, Daeweon, Kuryong and Cheongryong mines. Each of these mines occurs along copper-bearing hydrothermal quartz veins that crosscut late Cretaceous volcanic rocks, although some disseminated ores in host rocks also exist locally. Mineralization can be separated into three distinct stages (I, II, and III) which developed along preexisting fracture zones. Stage I is ore-bearing, whereas stages II and III are barren. The main phase of ore mineralization, stage I, can be classified into three substages (Ia, Ib and Ic) based on ore mineral assemblages and textures. Substage Ia is characterized by pyrite-arsenopyrite-molybdenite-pyrrhotite assemblage and is most common at the Hwacheon deposit. Substage Ib is represented by main precipitation of Cu, Zn, and Pb minerals. Substage Ic is characteristic of hematite occurrence and is shown only at the Kuryong and Cheongryong deposits. Some differences in the ore mineralization at each mine in the area suggest that the evolution of hydrothermal fluids in the area varied in space (both vertically and horizontally) with respect to igneous rocks relating the ore mineralization. Fluid inclusion data show that stage I ore mineralization mainly occurred at temperatures between ${\approx}350^{\circ}$ and ${\approx}200^{\circ}C$ from fluids with salinities between 9.2 and 0.5 wt.% eq. NaCl. In the waning period of substage Ia, the high temperature and salinity fluid gave way to progressively cooler, more dilute fluids of later substage Ib and Ic (down to $200^{\circ}C$, 0 wt.% NaCl). There is a systematic decrease in the calculated ${\delta}^{18}O_{H2O}$ values with paragenetic time in the Hwacheon-ri hydrothermal system from values of ${\approx}2.7$‰ for substage Ia, through ${\approx}-2.8$‰ for substage Ib, to ${\approx}-9.9$‰ for substage Ic. The ${\delta}D$ values of fluid inclusion water also decrease with decreasing temperature (except for the Daeweon deposit) from -62‰ (substage Ia) to -80‰ (substage Ic and stage III). These trends are interpreted to indicate the progressive cooler, more oxidizing unexchanged meteoric water inundation of an initial hydrothermal system which is composed of highly exchanged meteoric water. Equilibrium thermodynamic interpretation of the mineral assemblages with the variation in amounts of chalcopyrite through the paragenetic time, and the evolution of the Hwacheon-ri hydrothermal fluids indicate that the solubility of copper chloride complexes in the hydrothermal system was mainly controlled by the variation of temperature and $fo_2$ conditions.

• Animal Systematics, Evolution and Diversity
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• v.14 no.4
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• pp.341-355
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• 1998

Thirty one morphometric characters of specimens in 15 subspecies of striped field mouse(Apodemus agrarius Pallas) from Eurasia were analyzed to clarify taxonomic status of these subspecies. Five major subgroups in A. agrarius were revealed: I, a largest-size form, specimens from two southern from other six localities in Korea, subspecies coreae and pallescens; III, the other large-size from, specimens from Astrachan in western Russia, subspecies volgensis; IV, a medium-size form, specimens from 16 localities in eastern Asia(North Korea, China, and eastern Russia), subspecies coreae, manchuricus, pallidior, ningpoensis, and insulaemus; V, a small-size form, specimens from 16 localities in western Asia and Europe (Kazakhstan, Russia, Lithuania, and Ukraine), subspecies tianschanicus, ognevi, agrarius, septentrionalis, nikolski, caucasicus, and karelicus. From this morphometric analyses, the followings are concluded: subspecies chejuensis is a larger-size form, as noted by Johnson and Jones(1955): subspecies pallescens is the synonym of subspecies agrarius, as suggested by Koh(1986): subspecies coreae from Korea is a large-size form and is idistinct from other 12 subspecies in Eurasia: the eastern form of subspecies ningpoensis by Corbet(1978) is a medium-size form o subspecies manchuricus, pallidior, ningpoensis, and insulaemus from eastern Asia (China and eastern Russia), and it includea North Korea specimens: a small-size form from western Asia and Europe(subspecies tianschanicus, ognevi, agrarius, septentrionalis, nikolski, caucasicus, and karelicus) is the western form of subspecies agrarius by Corbet(1978); the other large-size form of subspecies volgensis from western Russia is a distinct subspecies, which differs from the western subspecies agrarius. Therefore, it is concluded that 15 subspecies of A. agrarius can be classifed into five subspecies (chejuensis, coreae, ningpoensis, agrarius, and volgensis), although it is necessary to measure and analyze morphometric characters of specimens of other seven subspecies(albostriatus, maculatus, rubens, kahmanni, henrici, gloveri, and harti) for the complete reclassification of this species).

• Animal Systematics, Evolution and Diversity
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• v.9 no.2
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• pp.151-170
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• 1993

Morphometric, band-pattern and electrophoretic analysis on Cobitis taenia complex were performed to investigate the morphological and genetic differentiation and to clarify their taxonomic status. Intermediate types of band-pattern (C and D type) were more frequently expressed than that of types of C. t. taenia(type A) and C. t. lutheri (type B). Sexual dimorphism of band-pattern was observed not only in C. t. taenia(type A) and C. t. lutheri(type B). Sexual dimorphism of band-pattern was observed not only in C. t. lutheri but also in C. t. taenia and C. t. striata as well. Discriminant function analysis based on 19 morphological characters shows no significant differences among C. taenia complex. The degree of genic variation of C. t. striata was higher ( =1.48, P=31.2%, HD=0.009) than those of C. t. striata was higher( =1.48, P=31.2%, HD=0.082 and HG=0.009) than those of C. t. lutheri ( =1.37, P=2.7%, HD=0.058 and HG=0.065). The average genetic similarities between C. t. lutheri and C. t. taenia-C. t. striata were S=0.62 and S=0.66 respectively and these values indicate that C. t. tanenia has evolved specific level of differentiation. C. t. striata and C. t. lutheri show subspecifc level of close genetic similarity (S=0.82). Based on the divergent time estimate (Nei, 1975) it is assumed that C. t. tanenia was branched off from the other subspecies about two million years before present (MYBP) and C. t. striata and C. t. lutheri were differentiated about 0.6 MYBP. The use of C. sinesis an the scientific name for the Korean C. t. taenia, proposed by Kim and Lee (1988) seems incorrect since they are quite different in the structure of lamina circularis (Vladycov, 1935), the external morphology and distribution (Cheng and Zheng, 1987) and the chromosome number(Yu et al., 1989). Kim and Lee(1988) also argued that C.t. striata and C. t. lutheri should be treated as distinct species but the present study and other reports (Kim and Lee, 1984; Kim and Yang, 1993) do not support it. We conclude that C. t. taenia is a good species and C. t. striata and C. t. lutheri are subspecific status. Their scientific names should be revised in the future.

• Animal Systematics, Evolution and Diversity
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• v.10 no.1
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• pp.97-123
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• 1994

The author devided th period of 1876-1945 into three epochs ; the Opening of Ports in 1876 -before the Political Reform in 1894 , the Political Reform- the Japanese annexation of Korea in 1910 , and the Epoch of Japanese Colony during 1910-1945. As civilization through including educational reform rised. The modern school system began to be introduced nongovernmentally and governmentally to Korea in the 1880's without any school laws. Were chronologycally established school regulation by Korea Government in 1895-1893, school laws by Korean Government under the supervision of the Japanese Residency-General of Korea in 1906-1910, and the educational laws of Korea by the Japanese Government-General of Korea in 1911-1943. In these epochs, the numbers of elementary , secondary and higher educational institutions and the numbers of pupils and students had increased slowly. Japanese had developed sonwhat primary education and secondary technical education, but it had checked extremely the Korean peoples to receive secondary liberal education and higher education, On the epoch of Japanese colony, Japanese occupied nearly half of elementary school teachers, almost of public secondary school teachers educated in Japan, and nearly all of professor educated in Japan in public and national colleges which were technical, and in one imperial university . Forty or more Korean teachers taught natural history chief at private secondary schools for Koreans , more than half of them being graduates of colleges of agriculture and forestry in Korea and Japan. The author mentioned curricula , and subjects and textbooks connected with biology of elementary, secondary and higher educational institutions. The pup8ls and students received biological knowledge through learning sciences at primary schools ; natural history (plants, animals and minerals ) at secondary schools including normal schools ; botany, zoology, genetics and major subjects related with biology such as anatomy, physiology, bacteriology, pland breeding at medical colleges and colleges of agriculture and forestry. There were no departments of biology , botany or zoology in Korea. Only seven Koreas graduated from departments of biology, botany or zoology at imperial universities in Japan. Some of them played the leading parts to develop education and researches of biology in the universities after 1945 Liberation.

• The Journal of the Petrological Society of Korea
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• v.21 no.2
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• pp.59-87
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• 2012

Recent studies reveal that eclogite formed in the Hongseong area and post collision igneous rocks occurred throughout the Gyeonggi Massif during the Triassic Songrim Orogeny. These new findings derive the tectonic model in which the Triassic Qinling-Dabie-Sulu collision belt between the North and South China blocks extends into the Hongseong-Yangpyeong-Odesan collision belt in Korea. The belt may be further extended into the late Paleozoic subduction complex in the Yanji belt in North Korea through the Paleozoic subduction complex in the inner part of SW Japan. The collision belt divides the Gyeonggi Massif into two parts; the northern and southern parts can be correlated to the North and South China blocks, respectively. The collision had started from Korea at ca. 250 Ma and propagated to China. The collision completed during late Triassic. The metamorphic conditions systematically change along the collision belt:. ultrahigh temperature metamorphism occurred in the Odesan area at 245-230Ma, high-pressure metamorphism in the Hongseong area at 230 Ma and ultra high-pressure metamorphism in the Dabie and Sulu belts. This systematic change may be due to the increase in the depth of slab break-off towards west, which might be related to the increase of the amounts of subducted ocecnic slab towards west. The wide distribution of Permo-Triassic arc-related granitoids in the Yeongnam Massif and in the southern part of the South China block indicate the Permo-Triassic subduction along the southern boundary of the South China block which may be caused by the Permo-Triassic collision between the North and South China blocks. These studies suggest that the Songrim orogeny constructed the Korean Peninsula by continent collision and caused the subduction along the southern margin of the Yeongnam Massif. Both the northern and southern Gyeonggi Massifs had undergone 1870-1840 Ma igneous and metamorphic activities due to continent collision and subduction related to the amalgamation of Colombia Supercontinent. The Okcheon metamorphic belt can be correlated to the Nanhua rift formed at 760 Ma within the South China blocks. In that case, the southern Gyeonggi Massif and Yeongnam Massif can be correlated to the Yangtz and Cathaysia blocks in the South China block, respectively. Recently possible Devonian or late Paleozoic sediments are recognized within the Gyeonggi Massif by finding of Silurian and Devonian detrital zircons. Together with the Devonian metamorphism in the Hongseong and Kwangcheon areas, the possible middle Paleozoic sediments indicate an active tectonic activity within the Gyeonggi Massif during middle Paleozoic before the Permo-Triassic collision.

• Animal Systematics, Evolution and Diversity
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• v.10 no.2
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• pp.157-187
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• 1994

This study includes the taxonomy , and a key to species of aphids in the family Lachnidae from Korea. Specimens examined in this study were collected from 24 kinds of plants. Samplings were accomplished at 95 localities in Korea from March, 1987 to August, 1994. A list of Korean lachnids are as follows. *1. Chinara atlantica (Wilson, 1919), *.2. C. cembrae(Seitner, 1936), *3. C. formosana (Takahashi, 1924), *4. C. fresai Blanchard, 1939, *5. C. idahoensis Knowlton,1935, 6. c. juniperi (de Geer, 1773), 7.C.kochi Inouye,1939, *8. C. laridicola (Matsumura, 1917), *9. C. laricis (Hartig, 2839), *10. C.longipennis (Matasumura, 1917), 11. C. orientalis (Takahashi, 1925), *12. C. pinidensiflorae(Essig & Kuwana, 1918), *13. C. piniformosana(Takahashi, 1923), *14 C. shinjii Inouye, 1938, *15. c. tujafilina (Del Guercio, 1909), *16 . c. watanabei Inouye, 1970, *17. C. togyuensis Seo. 1994. *18. C. deodarae Seo. 1994, *19. Eulachnus agilis (Kaltenbach, 1843), *20. E. pumilae Inouye, 1939, *21. E. thunbergi (Wilson, 1919), *22. Schizolachnus orientalis (Takahashi, 1924) , 23. Lachnus, Chosoni Szelegiewicz, 1975, 24. L. japonicus (Matsumura, 1917) , *.25. L. tropicalis 9van der Goot, 1916), *.26. Maculolachnus sumbacula (Walker, 1848), *27. M. paiki Seo. 1994, *28 Nipppolachnus piri Matsumura, 1917, 29. Stomaphis asiphon Szelegiewica, 1975, *30. S. japonica Takahashi, 1960, *31. S. yanonis Takahashi , 1918 , *32. Tuberolachnus salignus *(Gmelin, 1790). Of them , 27 species preceded by an asterisk were observed in this study, and keys to these 27 Korean lachnids are provided . The relationship between Korean lachnids and their host plants, and geogrpahical distribution are discussed.

• (The) Research of the performance art and culture
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• no.36
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• pp.289-312
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• 2018

Pansori has been passed down over several generations and over time have undergone continued change in accordance with the times, as well as the skills and ability of the singer. Policies regarding intangible cultural treasures were established to preserve and promote the continuing tradition of art forms including Pansori and thus must spare no effort in supporting and preserving the genre. As such, for proper implementation of the newly legislated law, it is necessary to review the agents who pass down the tradition of Pansori and whether there are any areas that need to be changed in terms of our perception of culture in general. Pansory in the $21^{st}$ century features both contemporary aspects and mass appeal and have undergone many changes in how it is enjoyed. It is our responsibility therefore, to establish how the art and universality of Pansori should be promoted. From this perspective, this study reviewed the evolution of law on intangible cultural treasures, the current status of intangible cultural treasures being passed down with a focus on national treasures and those of Jeonbuk Province to shed light on issues. Diversification is needed in the number of those who carry this intangible cultural treasure, as well as the number of categories. To that end, a survey index or practical ability index must be taken into account for the application and designation of intangible cultural treasures. The study also noted issues of the categories for designation as intangible cultural treasures and discussed directions for improvement. In the case of Pansori, suggestions for improvement were presented for the designation of skilled artists by school, regular surveys and regular application, increased role of artists for increased mass appeal, survey of regional singers, supervision and monitoring of skilled artists and establishment of a manual for the education on how to pass down the art form. In doing so, efforts should be made to make the passing down of Pansori more active and related education more systematic. Since we are in the early years of the law on intangible cultural treasures being implemented, areas of improvement will continue to be identified. It is however certain that the proper support for the art form to be handed down should be done in a way where law and culture are complementary given that Pansori is not just a Korean tradition, but a tradition of mankind.

• Journal of Intelligence and Information Systems
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• v.28 no.2
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• pp.207-235
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• 2022

In a knowledge-based society where knowledge and information industries are the main pillars of the economy, knowledge sharing and diffusion and its systematic management are recognized as essential strategies for improving national competitiveness and sustainable social development. In the field of Information Systems (IS) research, where the convergence of information technology and management takes place in various ways, the evolution of knowledge occurs only when researchers cooperate in turning old knowledge into new knowledge from the perspective of the scientific knowledge network. In particular, it is possible to derive new insights by identifying topics of interest in the relevant research field, applied methodologies, and research trends through network-based interdisciplinary graftings such as citations, co-authorships, and keywords. In previous studies, various attempts have been made to understand the structure of the knowledge system and the research trends of the relevant community by revealing the relationship between research topics, methodologies, and co-authors. However, most studies have compared two or more journals and been limited to a certain period; hence, there is a lack of research that looked at research trends covering the entire history of IS research. Therefore, this study was conducted in the following order for all the papers (from its first issue in 1977 to the first quarter of 2022) published in the MIS Quarterly (MISQ) Journal, which plays a leading role in revealing knowledge in the IS research field: (1) After extracting keywords, (2) classifying the extracted keywords into research topics, methodologies, and theories, and (3) using topic modeling and keyword network analysis in order to identify the changes from the beginning to the present of the IS research in a chronological manner. Through this study, it is expected that by examining the changes in IS research published in MISQ, the developing patterns of IS research can be revealed, and a new research direction can be presented to IS researchers, nurturing the sustainability of future research.