• Title/Summary/Keyword: SUN: UV

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Multiple functions of human UV DNA repair endonuclease III

  • Jang, Chang-Young;Kim, Joon
    • Journal of Photoscience
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    • v.9 no.2
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    • pp.182-185
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    • 2002
  • There are 3 UV DNA repair endonuclease activities in mammalian cells that cleave UV -irradiated DNA. Interestingly, mammalian UV endonuclease III with MW of 26.7kD has a lyase activity on AP sites. It also cleaves the phosphodiester bond within a cyclobutane pyrimidine dimer. Genomic analysis of human repair endonuclease III gene revealed that this gene has 100% sequence identity with ribosomal protein S3 (rpS3). Therefore, rpS3 seems to function both in translation and in DNA repair. This gene of about 6.1 kb contains 6 introns and 7 exons, and the first and fifth introns of human rpS3 gene contain functional U15 small nucleolar (sno) RNAs which appear to be involved in ribosome assembly. It is to be noted that the column profile of the endonuclease activity of rpS3 appears to be altered in Xeroderma Pigmentosum (XP) group D cells compared to normal cells indicating that this protein is involved in XP disease as well. XP is a human disease characterized by high sensitivity of skin by UV- or sun-light irradiation and by high frequency of developing skin cancers. We also report here that rpS3 protein is involved in other cellular functions.

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Continuous Surface Treatment and Dyeability of PTT Film via $UV/O_3$ Irradiation (UV/Ozone 조사에 의한 PTT 필름의 연속식 표면처리와 염색성)

  • Jang Jinho;Park Dae Sun
    • Textile Coloration and Finishing
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    • v.17 no.1
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    • pp.7-13
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    • 2005
  • Continuous and intense UV irradiation on PTT film using two types of UV bulbs at different irradiation power level was carried out to modify surface characteristics of the film including zeta potential, wettability, surface energy, and dyeability. ESCA analysis of the irradiated film showed higher O/C ratio than the untreated film indicating photooxidation of outer surface layer. ATR analysis showed that the ester bonds were broken and some new groups were produced such as carboxylic acid, phenolic hydroxy, and other esters, implying that ester bonds of PTT was responsible for the observed photooxidation effect. The surface of the treated PTT film became more hydrophilic and wettable to water, coupled with increased surface energy. Polar component of the surface energy increased and nonpolar component decreased with increasing irradiation energy. The treatment also decreased zeta potential of the modified surface and nanoscale roughness increased with increasing irradiation. The dyeability of the treated films to catonic dyes was significantly improved by electrostatic and polar interaction between dye molecules and the anionic film surface. The UV irradiation seems to be a viable polymer surface modification technology, which has advantages such as no vacuum requirement and continuous process unlike plasma treatment.

Quantitative Analysis of Bioactive Marker Compounds from Cinnamomi Ramulus and Cinnamomi Cortex by HPLC-UV

  • Jeong, Su Yang;Zhao, Bing Tian;Moon, Dong Cheul;Kang, Jong Seong;Lee, Je Hyun;Min, Byung Sun;Son, Jong Keun;Woo, Mi Hee
    • Natural Product Sciences
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    • v.19 no.1
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    • pp.28-35
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    • 2013
  • In this study, quantitative and pattern recognition analysis for the quality evaluation of Cinnamomi Ramulus and Cinnamomi Cortex using HPLC/UV was developed. For quantitative analysis, three major bioactive compounds were determined. The separation conditions employed for HPLC/UV were optimized using an ODS $C_{18}$ column ($250{\times}4.6$ mm, 5 ${\mu}m$) with gradient conditions of acetonitrile and water as the mobile phase, at a flow rate of 1.0 mL/min and a detection wavelength of 265 nm. This method was fully validated with respect to linearity, accuracy, precision, recovery, and robustness. The HPLC/UV method was applied successfully to the quantification of three major compounds in the extract of Cinnamomi Ramulus and Cinnamomi Cortex. The HPLC analytical method for pattern recognition analysis was validated by repeated analysis of thirty eight Cinnamomi Ramulus and thirty five Cinnamomi Cortex samples. The results indicate that the established HPLC/UV method is suitable for quantitative analysis.

Raman spectroscopy study on the reactions of UV-generated oxygen atoms with single-layer graphene on SiO2/Si substrates

  • Ahn, Gwang-Hyun;Kim, Hye-Ri;Hong, Byung-Hee;Ryu, Sun-Min
    • Carbon letters
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    • v.13 no.1
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    • pp.34-38
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    • 2012
  • Successful application of graphene requires development of various tools for its chemical modification. In this paper, we present a Raman spectroscopic investigation of the effects of UV light on single layer graphene with and without the presence of $O_2$ molecules. The UV emission from a low pressure Hg lamp photolyzes $O_2$ molecules into O atoms, which are known to form epoxy on the basal plane of graphene. The resulting surface epoxy groups were identified by the disorder-related Raman D band. It was also found that adhesive residues present in the graphene samples prepared by micro-mechanical exfoliation using adhesive tape severely interfere with the O atom reaction with graphene. The UV-induced reaction was also successfully applied to chemical vapor deposition-grown graphene. Since the current method can be readily carried out in ambient air only with UV light, it will be useful in modifying the surfaces of graphene and related materials.

Thermal Melt Grafting of Maleimides Having UV-absorber onto Polypropylene (폴리프로필렌에 UV 흡수제를 갖는 말레이미드의 용융그래프팅)

  • Kim, Taek Hyeon;Na, Hye-Sun
    • Polymer(Korea)
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    • v.38 no.1
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    • pp.49-53
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    • 2014
  • A novel monomeric UV-absorber was prepared by the reaction of 2,4-dihydroxybenzophenone with N-4-chlorocarbonylphenylmaleimide in the presence of triethylamine. This reactive maleimide was grafted onto polypropylene (PP) by the thermal melt-processing in a mini-max-moulder. IR spectroscopic method was used for the quantitative determination of the extent of grafting of monomeric maleimide. To find the optimal reaction conditions, the dependence of reaction temperature and time and the concentration of monomeric UV-absorber was investigated on the grafting yields. The photooxidative effect of the grafted PP was evaluated in the weatherometer comparing to the PP mixed with UV-absorbers using the carbonyl index of IR spectrum. The grafted PP showed an excellent anti-photooxidative effect.

Forward Gene Mutation Assay of Seven Benzophenone-type UV Filters using L5178Y Mouse Lymphoma Cell

  • Jeon, Hee-Kyung;Sarma, Sailendra Nath;Kim, Youn-Jung;Ryu, Jae-Chun
    • Molecular & Cellular Toxicology
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    • v.3 no.1
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    • pp.23-30
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    • 2007
  • The effects of high energy short wave solar radiation on human skin have received much publicity as the major cause of accelerated skin ageing and of skin cancers. To meet public demand, the cosmetic industry has developed sun protection factor products, which contain a variety of so-called "UV filters", among others benzophenone (BP) and its metabolites are the widely used UV filters. UV filters are also used to prevent UV light from damaging scents and colors in a variety of cosmetics products and to protect of plastic products against light-induced degradation. There are many variants of BP in use. In this respect, to regulate and to evaluate the hazardous effect of BP-type UV filters will be important to environment and human health. The genotoxicity of 7 BP-type UV filters was evaluated in L5178Y $(tk^{+/-})$ mouse lymphoma cells in vitro. BP, benzhydrol, 4-hydroxybenzophenone 2-hydroxy-4-methoxybenzophenone and 2, 4-dihydroxybenzophenone did not induce significant mutation frequencies both in the presence and absence of metabolic activation system. 2, 2'-Dihydroxy-4-methoxybenzophenone appeared the positive results at the highest dose, i.e. 120.4 ${\mu}g/mL$ only in the absence of metabolic activation system. And also, 2, 3, 4-trihydroxybenzophenone revealed a significant increase of mutation frequencies in the range of 138.1-207.2 ${\mu}g/mL$ in the absence of metabolic activation system and 118.3-354.8 ${\mu}g/mL$ in the presence of metabolic activation system. Through the results of MLA with 7 BP-type UV filters in L5178Y cells in vitro, we may provide the important clues on the genotoxic potentials of these BP-type UV filters.

The Characteristics of UV Generation and Aging Materials in According to Surface Discharge (연면방전에 의한 폴리머애자의 자외선 발생과 열화특성)

  • Shong, Kil-Mok;Bang, Sun-Bae;Kim, Chong-Min;Kim, Young-Seok;Jung, Jin-Su
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.57 no.9
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    • pp.1606-1611
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    • 2008
  • Recently, The diagnosis techniques of electric facilities are developed on live line. This paper describes the discharge characteristics of polymer insulator(EPDM A type). Keeping the facilities in good working order, the goal of this paper will provide the information to enable user to easily judge conditions of facilities on the spot. The performance of polymer insulator is assessed from the KS C IEC 60270 and CEA LWIWG-01(96)(Tracking Wheel Test). As the results, UV generation patterns of polymer insulator grow like a jellyfish shape follow the ramping voltage. Discharging UVs are detected at about 31.25% of breakdown voltage. Just then, dimension of UV image is about $0.84cm^{2}$. The dimension of max. UV image is about $297.4cm^{2}$ at $160kV(V_{bd})$. The position of UV generation due to surface discharge of polymer insulator is the center of insulator in the early, then moved the ground side and the last, UV image moved through the junction part of source side. Surface of aged polymer insulator is cracked and faded due to arc. UV absorption spectrum of polymer insulator are appeared the C-H bond of scissoring vib. at $1014cm^{-1}$ and C=O bond of recombination structure. Also, recombined UV absorption peak such as C-H, N-H, and O-H is detected at the $3321cm^{-1}$. Through the paper, there are inspection data which are the relations between surface discharge of polymer insulator and UV detecting image.

Photoinduction of UV-absorbing Compounds and Photo-protective Pigment in Phaeocystis pouchetii and Porosira glacialis by UV Exposure (실내 자외선 노출 실험을 통한 극지 식물플랑크톤(Phaeocystis pouchetii, Porosira glacialis)의 자외선 흡수물질 생성 연구)

  • Ha, Sun-Yong;Kang, Sung-Won;Park, Mi-Ok;Kim, Young-Nam;Kang, Sung-Ho;Shin, Kyung-Hoon
    • Ocean and Polar Research
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    • v.32 no.4
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    • pp.397-409
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    • 2010
  • Herein, we compared the production rate of UV-absorbing compounds (mycosporine-like amino acids) and carotenoids in two phytoplankton species--Phaeocystis pouchetii and Porosira glacialis--which are the dominant species in Polar Regions under artificial UV radiation conditions. P. pouchetii exposed to UVR and PAR evidenced reductions in the carbon fixation rate, and was not significantly influenced by differing light conditions. However, the concentrations of UV-absorbing compounds and photo-protective pigments of P. pouchetii were increased with increasing exposure time, but P. glacialis maintained constant levels during the UVR exposure experiment. The production rates of MAAs showed a reverse phase between the two phytoplankton species. The carbon fixation rate of P. pouchetii cells was inhibited by exposure to UV radiation, but the production rates of MAAs in P. pouchetii were increased under UV radiation exposure. The carbon fixation rate and production rate of MAAs in P. glacialis were simultaneously inhibited under UV radiation exposure conditions. These results provide us with new information regarding the processes involved in the production of UV-absorbing compounds and photoprotective pigments in two phytoplankton species.

The Applicability Evaluation of UV Indicators in the Exhibition Environment (전시환경에 대한 자외선 지시카드 적용성 평가)

  • Lim, BoA;Shin, EunJeong;Kim, MyoungNam;Lee, SunMyung
    • 보존과학연구
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    • s.33
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    • pp.85-97
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    • 2012
  • In this study, reliability assessment was carried out on UV(ultraviolet) indicators for the application of the indicator method on cultural heritages. As a result, some UV indicators(L, K, and M) proved to be unsuitable due to their low reactivity to UV and high reactivity to RH(relative humidity). On the other hand, indicator S showed considerable color changes and fading due to UV-B and UV-C according to the integral amount of UV irradiation. Indicator S showed the most distinct reaction to UV and a remarkable applicability to exhibition light, which are used in museums. Therefore, UV indicator S is an easy and useful monitoring tool for light environment management in cultural- property conservation facilities.

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The UV LED Bar Optimal Design with Human Detection and Control Function (인체 감지 제어 기능을 갖는 UV LED Bar의 최적 설계)

  • Kim, Chang-Sun;Lee, Jae-Hak;Goh, Young-Jin
    • The Journal of the Korea institute of electronic communication sciences
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    • v.12 no.6
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    • pp.1219-1226
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    • 2017
  • In this paper, it is performed the optimal design of the UV LED bar which can be used variously. The UV LED Bar emits ultraviolet rays, so it is important to emit ultraviolet rays constantly for the purpose of use. In order to emit a certain amount of ultraviolet rays as ever, the ultraviolet ray emission should be driven by a constant current source within the operable input voltage range. And also the heat dissipation is particularly important because of the long ultraviolet emission retention time due to the UV utilization characteristics. In addition, since human body protection is essential, the algorithm is configured to operate according to human body detection using distance sensor and Bluetooth. Three 365nm UV LEDs were used in series to emit ultraviolet UVA, operating at the constant current of 500mA with an efficiency of 87.5% and a power consumption of 6.006W. The ultraviolet radiation dose was measured at $5.35mW/cm^2$ at the distance of 10 cm when measured by the Lutron ultraviolet measuring instruments.