• Journal of the Korean Society for Railway
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• v.12 no.2
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• pp.243-248
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• 2009

The operation of KTX in Korea has launched since 2004, which brought about a great change in railroad industry. We have acquired lots of great know-hows, especially in railroad construction and operation field. Therefore, we have been building up numerous operation skills until now. However, it was necessary to investigate some of the equipment based on our own environment because the high speed train system were totally imported. In case of Toughened Glass Stem Insulator, it was totally new type in domestic. This paper introduces estimation method and result in three different field aspect, such as an electrical field, a physical field, and environmental circumstances. First, in case of an electrical field, amplitude and a number of time for switching surge voltage data were collected. Second, in case of a physical field, amplitude and trend of vibration in to the insulator were examined. Finally, in case of environmental circumstances, flying possibility of gravel and ice clod were investigated. Also shrink and expend characteristic according to temperature were reviewed. Through this basic data, suitability for Toughened Glass Stem Insulator using in domestic high speed railway have been accumulated and estimated.

• The Journal of Korean Association of Computer Education
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• v.22 no.3
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• pp.37-54
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• 2019

The main purpose of STEM education is to understand methods of inquiry in each discipline to develop convergent problem solving skills. To do this, we must first understand the problem-solving process that is regarded as an essential component of each discipline. The purposes of this study is to understand the relationship between the problem solving in science (S), engineering (E), mathematics (M), and computational thinking (CT) based on the comparative analysis of problem solving processes in each SEM discipline. To do so, first, the problem solving process of each SEM and CT discipline is compared and analyzed, and their commonalities and differences are described. Next, we divided the CT into the instrumental and thinking skill aspects and describe how CT's problem solving process differs from SEM's. Finally we suggest a model to explain the relationship between SEM and CT problem solving process. This study shows how SEM and CT can be converged as a problem solving process.

• Reproductive and Developmental Biology
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• v.31 no.3
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• pp.215-220
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• 2007

This study was conducted to examine the establishment of bovine ES-like cells having pluripotency. The hatched blastocysts derived from culture of in vitro fertilized embryos for 10 to 12 days dissociated mechanically into ICM-and trophectoderm-rich clumps using needle, and cultured onto mitotically-inactivated MEF feeder layer. The primary colonies originated from ICM cells were detached mechanically 7 days after seeding and subsequent subculture was conducted at intervals of every 5 to 7 days. Two ES -like cell lines were established and maintained over 40 passages. Self-renewal of the established lines was confirmed by examining the alkaline phosphatase activity, stem cell-specific marker profiles including SSEA isotopes, Oct-4 and STAT3. Moreover, the established cell lines could produce anchorage-independent embryoid bodies (EBs) with gradual decrease of Oct-4 transcript level in time-dependent manner.

• Korean Journal of Clinical Laboratory Science
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• v.47 no.3
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• pp.117-124
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• 2015

Mesenchymal stem cells (MSCs) are an attractive tool in tissue engineering as they have the required potential to treat injured articular cartilage. UV-exposed DTOPV (S-triazine bridged p-phenylene vinylene) is a biocompatible and fluorescent polymer with a hydrophilic surface. Previous studies have demonstrated that the surface wettability and hydrophilicity play critical roles in regulating cell adhesion and proliferation. The objective of this study was to improve the potential of in vitro MSC differentiation into Chondrocytes using DTOPV. MSCs were cultured on two different substrates: (1) tissue culture polystyrene (TCPS) as a reference and (2) UV-exposed and patterned DTOPV films. Chondrogenesis of MSCs was induced for two weeks on TCPS and DTOPV in the presence of an induction medium containing transforming growth factor (TGF)-${\beta}3$. Interestingly, the MSCs on TCPS adhered and spread, while those on DTOPV tended to form aggregates within several days. The cells cultured on DTOPV for two weeks had a round morphology, with stronger Safranine O staining of the extracellular matrix than that of the cells cultured on TCPS. Also, Type II collagen gene was significantly expressed in cells induced on DTOPV. These results indicate that chondrogenic differentiation of MSCs proceeds more rapidly on DTOPV than on TCPS. Therefore, in cartilage tissue engineering, DTOPV could be used to induce effective chondrogenic differentiation of MSCs.

• Journal of the korean academy of Pediatric Dentistry
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• v.42 no.4
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• pp.312-318
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• 2015

The aim of this study was to investigate the possibility of the supernumerary teeth for the stem cell source in dentistry. The Real Time Quantitative Reverse Transcription Polymerase Chain Reaction (Real Time qRT-PCR) method was used to evaluate the differentiation toward the odontoblast of the supernumerary dental pulp stem cells (sDPSCs). Supernumerary dental pulp stem cells were obtained from 3 children (2 males and 1 female, age 7 to 9) diagnosed that the eruption of permanent teeth was disturbed by supernumerary teeth. The common genes for odontoblasts are alkaline phosphatase (ALP), osteocalcin (OC), osteonectin (ON), dentin matrix acidic phosphoprotein 1 (DMP-1), dentin sialophosphoprotein (DSPP). The sDPSCs were treated for 0 days, 8 days and 14 days with additives and then Real Time qRT-PCR was performed in intervals of 0 days, 8 days and 14 days. The alizarin-red solution staining was performed to visualize the stained color for the degree of calcification at 7 days, 14 days, 21 days and 28 days after treating additives to the sDPSCs. From the result of the Real Time qRT-PCR, the manifestation exhibit maximum value at 8 days after additive treatment and shifted to a decrease trend at 14 days. Alizarin-red solution staining exhibit light results at 7 days after staining and generalized dark result at 14 days. Consequently, in studies with sDPSCs, appropriate treatment time of additives for Real Time qRT-PCR is 8 days. Also, a suitable period of Alizarin-red solution staining is 14 days.

• Microbiology and Biotechnology Letters
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• v.39 no.4
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• pp.364-373
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• 2011

Human embryonic stem cells have been highlighted as a valuable cellular source in the regenerative medicine field, due to their pluripotency. However, there is the challenge of the establishment of specific functional cell type forms of undifferentiated human embryonic stem cells (hESC). To establish and purify functional cell types from hESCs, we differentiated undifferentiated hESCs into vascular lineage cells and sorted the specific cell population from the whole cell population, depending on their cell volume, and compared them with the non-sorted cell population. We observed that about 10% of the PECAM positive population existed in the VEGF induced differentiating human embryoid body (hEB), and differentiated hEBs were made into single cells for cell transplantation. After making single cells, we performed cell sorting using a fluorescence-activated cell sorter (FACs), according to their cell volume on the basis of FSC region gating, and compared their therapeutic capacity with the non-sorted cell population through cell transplantation into hindlimb ischemic disease model mice. 4 Weeks after cell transplantation, the recovery rate of blood perfusion reached 54% and 17% in the FSC regions of sorted cells- and non-sorted cells, respectively. This result suggests that derivation of a functional cell population from hESCs can be performed through cell sorting on the basis of cell volume after preliminary differentiation induction. This approach may then greatly contribute to overcoming the limitations of marker sorting.

• Proceedings of the Korean Society for Information Management Conference
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• 2013.08a
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• pp.15-18
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• 2013

본 연구는 최근에 발표된 단위 논문별 영향력을 예측하기 위해 해당 저자가 이전에 발표했던 논문들의 년도별 피인용 추이를 분석하였다. 최근 IF가 가장 높은 "Cell & Tissue Engineering" 분야와 그 분야에서 IF가 가장 높은 저널 "Stem Cell"을 선정하여 최근에 실린 논문의 저자정보를 파악하고, 해당 저자의 이전에 발표한 논문들을 추출하였다. 분석 결과, 저자가 발표한 논문들이 최근에 인용이 많이 일어나면 이후 발표한 논문의 인용빈도가 높아지는 것으로 나타났으며, 특히 동일한 h-index를 가진 저자들 간의 비교에서 더욱 두드러지게 나타났다.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.28 no.1
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• pp.249-267
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• 2018

The US cybersecurity workforce policy is being pursued comprehensively and systematically, based on the NICE established initiated in 2010. Security Technologies, one of the eight areas of Advanced Technology Education(ATE) of the National Science Foundation(NSF) included in the STEM. This policy has been comprehensively promoted in conjunction with NICE, and this security technology field is operated with five detailed programs. In this paper, we examine in detail five cybersecurity workforce development programs supported by ATE, and compare them with the current status cultivation of cybersecurity workforce in Korea. After finding out the problems and improvements by comparison with the current situation of cybersecurity workforce development in Korea, we propose several implementations of nation-wide strategies for cultivating new cybersecurity workforce in Korea.

• Journal of Convergence for Information Technology
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• v.9 no.10
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• pp.114-121
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• 2019

The purpose of this study was to examine the effects of the undergraduate research program(URP) among Korean students. URP is frequently implemented in STEM(Science, Technology, Engineering, Math) majors, and this study tested whether the participation of URP was effective in convergence competency, major satisfaction, and academic achievement. Data were collected from 101 participants and 117 non-participants of URP at an A university. The research results showed that the mean scores of convergence competency, major satisfaction, and academic achievement of the URP participating group were significantly higher than those of the non-participating group.

• Journal of Integrative Natural Science
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• v.1 no.3
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• pp.234-246
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• 2008

Korean terminologies on stem structures in plant morphology, written incorrectly in many books, were analysed to propose accurately expressed terminologies. 35 books in areas such as general biology, plant biology, plant morphology, and biological dictionaries and glossaries were selected to analyse the accuracy of the terminologies for seed structures, e.g., shoot and shoot system, rhizome, apical dominance, anticlinal and periclinal divisions, and intercalary and lateral meristems. The definition and etymology of the terminologies were traced in 4 textbooks of plant anatomy and 2 dictionaries of biology and botany written in English. On the basis of the definition, etymology, and principles for terminology formation according to the International Organization for Standardization (ISO 704:2000), reasonably expressed Korean terminologies were proposed. All of the 8 terminologies examined in this study were included in the glossary of biological terminologies, published by the Korean Association of Biological Sciences in 2005, and designated as an editorial source for science and biology textbooks for middle and high schools by Ministry of Education in 2007. However, the only 1 of the 8 terminologies described in the glossary were consistent with the proposed expression in the present study. These inconsistencies indicated the need for a reassessment of this glossary of biological terminologies. The validity of the proposed Korean terminologies was tested in a questionnaire sent to 17 professors teaching plant morphology or/and taxonomy at universities. A mean of 91.9% of the total respondents agreed with the Korean expressions proposed in this study. The new, proposed terminologies would facilitate mutual understanding between teachers and students of plant biology.