• 한국가축번식학회지
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• 제17권4호
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• pp.279-285
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• 1994

The objective of the present study was to characterize the cell cycles of granulosa cell populations during follicular maturation in cattle by using flow cytometer. Granulosa cells were isolated from bovine preovulatory antral follicles of F1(>10mm), F2(5~20mm), F3(3~4mm) and F4(1~2mm) diameter and fixed and stained with fluorochromes that selectively bine to DNA. Flow cytometer equipped with a laser excitation system was used to analyze the intensity of fluorescence from stained cells. Forward angle light-scatter(FSC) and 90$^{\circ}$light-scatter(SSC) signals were adopted to measure the size and the granularity of granulosa cells. As a results of FSC/SSC analysis, granulosa cell populations(G1 phase of cell cycle) from each follicle were relatively regular in size and granularity, regardless of follicular size. However, their distribution in granularity was greater than that in size. Most of granulosa cell populations collected from each follicle were distributed in G0/G1, S and G2/M phases. As the follicles approached to ovulation the percentage of cells in the proliferative phases of cell cycle (S and G2/M) decreased significantly, but there was a concomitant increase in the percentage of granulosa cells in G1 phase. Therefore, these data indicate the proportion of main populations to cell cycle of granulosa cells may be changed from proliferative phase to G1 phase during follicular maturation in cattle.

• Journal of Biosystems Engineering
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• 제36권2호
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• pp.130-139
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• 2011

Three modes of VIS/NIR spectroscopic measurement (interactance and two modes of transmission) were compared for their ability to estimate soluble solids content (SSC) of 'Shingo' pear non-destructively. The two transmission modes are named as full- and semi-transmission, where full-transmission stands for passing of light through abdomen of pear and semi-transmission is for transit of light mainly through flesh of pear. For comparison of the modes, prediction models developed from the collected spectroscopic data by the three modes were developed and tested for comparison of their performance. Partial least square regression (PSLR) was used to develop the models and various pre-processing methods were applied to develop models of high accuracy. The experiment was repeated three times with pears produced in different regions. The experiments resulted that selection of pre-processing is very important to attain accurate models, and multiplicative scatter correction (MSC) was selected as a pre-processor of high accuracy for the three modes of spectroscopic measurement in every experiment. Except for MSC, different group of pre-processing methods were selected for the three modes of measurement in every experiment without any tendency to the tested modes of measurement and pears of different produced region. Root-mean-square error of prediction (RMSEP) of prediction models of the three modes of measurement using prepreocessor of MSC were compared for their ability to estimate SSC. The models resulted in ranges of $0.37{\sim}0.57^{\circ}Brix$, $0.65{\sim}0.72^{\circ}Brix$, $0.39{\sim}0.51^{\circ}Brix$ for interactance, full- and semi-transmission, respectively. As shown, modes of semi-transmission and interactance resulted about the same level of prediction accuracy and were noted as modes of high performance to predict SSC.

• 보존과학회지
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• 제37권6호
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• pp.821-830
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• 2021

비무장지대에서 수습한 총기류 유품은 매장된 상태로 있다가 출토되는 순간 급격한 환경변화로 인해 부식에 취약하다. 특히 철제 총기류에 있어서 활성부식을 일으키는 염소이온(Cl^-)은 반드시 제거되어야 하지만 근현대에 제작되었다는 재질 특성과 매장기간을 고려하였을 때 고고유물과 동일한 탈염조건을 적용하는 것은 어려움이 있다. 그러므로 본 연구에서는 비무장지대 내 6·25 한국전쟁 전사자 유해발굴 현장에서 수습한 총기류를 대상으로 보존처리와 탈염 실험을 실시하였으며, 탈염 실험은 탈이온수와 소디움 세스퀴카보네이크 용액에 온도별로 침적시키는 방법을 서로 비교하여 가장 안정적인 보존방안을 모색하였다. 탈염 실험을 통해 탈염처리 방법에 따른 염소이온의 추출정도를 비교 실험한 결과, 100℃ 소디움 세스퀴카보네이트에서 가장 많은 용출량을 보였으며 탈염기간이 1~2주로 매우 짧은 결과를 보였다. 따라서 나머지 유품 중 총신과 방아틀 뭉치는 100℃로 가열하여 8시간, 상온에서 16시간씩 매주 1회 약품을 교체하는 방법으로 총 6회 탈염처리를 실시하였다. 그러나 탄약이 장전된 총신의 경우 탈염처리 시 고온 및 진공함침으로 인한 압력으로 폭발의 위험성을 고려하여 탈염처리와 진공함침 강화처리를 실시하지 않았다.

• 대한토목학회논문집
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• 제43권3호
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• pp.321-335
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• 2023

자연하천에서의 유사량 계측은 하천공학적으로 중요한 의미를 가지지만 계측 방법의 비용 문제로 유사량 실측에 어려움이 따른다. 특히 소류사량 계측의 어려움으로 인해 주기적인 유사량 모니터링의 대부분이 부유사 농도 계측에만 제한되어 있는 실정이다. 본 연구에는 자동유량관측소에 설치된 횡방향 도플러 유속계(H-ADCP)의 후방산란값과 부유사 농도의 상관관계를 이용해 실시간으로 부유사 농도를 산정하고 총유사량을 산정하는 서포트벡터회귀 모형을 제안한다. 제안하는 실시간 총유사량 모니터링 시스템은 부유사 농도 모형과 수정 아인슈타인 방법을 모사하는 총유사량 산정 모형으로 구성된다. 각 모형의 매개변수와 입력변수는 K겹 교차검증 기반 격자검색 방법과 재귀적 특징 제거법을 이용해 결정되었다. 교차검증에서 부유사 농도 모형과 총유사량 산정 모형의 R²가 각각 0.885와 0.860으로 유사량-유량 관계곡선에 비해 정확한 것으로 나타났다. 시계열 유사량 관측을 통해 새로 제시되는 실시간 총유사량 관측 시스템이 자연하천에서 발달하는 유사량-유량 이력관계와 미세한 유량 변화에서 나타나는 유사량 변화를 성공적으로 관측할 수 있음을 확인했다. 본 연구에서 제안하는 방법은 마찰경사나 부유사 입도 등의 수리 조건을 가정할 필요 없이 H-ADCP의 원시자료만으로 부유사 농도와 총유사량을 산정할 수 있어 기존 방법에 비해 불확도가 적으며 경제적이다. 본 방법은 H-ADCP가 설치된 유사량 관측소에 광범위하게 적용 가능해 유사량 모니터링의 시간적 해상도를 경제적으로 크게 줄일 수 있을 것으로 기대된다.

• 원예과학기술지
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• 제30권2호
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• pp.158-161
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• 2012

The influence of lysophosphatidylethanolamine (LPE) on anti-senescence of melon leaves and the change in fruit quality during the storage at low temperature were studied. In most of the crops, freshness of leaves is important factor for characteristics of fruits, such as sugar contents, color, and firmness. Melon ($Cucumis$ $melo$ L. cv. Prince) plants were sprayed with LPE at 5 and 3 weeks before commercial harvest. In upper part, LPE treatment showed slight high number of fresh leaf compared to no treatment (None). However, in lower part, LPE resulted in apparent inhibition effect on senescence, showing that lower side of melon plant kept fresh upon LPE application up to about 30%. The SSC of melon treated with LPE was similar to that of fruit from None at harvest. There was no change in soluble solids content (SSC) for all treatment during the storage at $7^{\circ}C$. There were no significant differences in firmness of mesocarp from melons given different treatments at harvest. The firmness of mesocarp from melon treated with LPE was higher than none after 2 weeks storage. The electrolyte leakage means for melon treated with LPE did not differ significantly from the means at initial storage after 2 weeks storage among the treatments. None increased 57% from its initial electrolyte leakage during storage. These results suggest that the application of LPE may have potential to inhibit senescence of leaves and maintain fruit quality during the storage in melon.

• 대한수의학회지
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• 제22권2호
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• pp.99-109
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• 1982

Morphological changes of the oviductal epithelium of the rat treated with hormones ($17{\beta}$-estradiol ${\mu}g$/day and progesterone 2.5mg/day) for ten days were observed transmission and scanning electron microscopically. The results obtained were as follows: 1. The cilia formation of ciliary cell(CC) was more accelerated by the treatment of estradiol than progesterone, but the balance of estrogen and progesterone was required for the maintenance of CC. The effect of hormone was different between the segments for the maintenance of CC. 2. The short secretory cell(SSC) was severely inhibited in the formation of secretory granules with single hormonal treatment but the activity of secretion was more inhibited by progesterone than by estradiol. 3. The long secretory cell(LSC) had not a great difference between estradiol and progesterone treatments as compared with the normal sexual cycle, but the formation of secretory granules was somewhat accelerated by progesterone treatment. 4. The formation of secretory granules of junctional cell (JC) was severely accelerated by estradiol treatment as compared with the normal sexual cycle. The formation of secretory granules during progesterone treatment, on the other hand, was inhibited completely, but the numbers of pinocytotic vesicles appeared at the cytoplasmic apical portion. 5. Three types of secretory cells, SSC, LSC and JC, on the rat oviductal epithelium could be suggested to have different cell tapes respectively from the morphological changes by hormone treatment.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 1993년도 학술발표집 지반진동 영향평가
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• pp.15-54
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• 1993

미국 테네시주 채펠 힐 북쪽 3.2km, 한 철로변에서 지반진동을 계측하였다. 6일동안 18회의 열차 주행중에 이 계측을 수행하였다. 이 연구의 목적은 열차나 다른 교통수단에 의한 SSC(Superconducting Super Collider) 설치 예상 깊이에서 지반 진동 크기를 분석 평가하는 데 있다. 이 지반 진동과 비교할 .기준치는 매우 엄밀히 설정된 간이다 . 즉, 주파수 3 Hz에서 0.0005인치(0.0127mm)이다. 이 계측 결과로는, 지하 100ft(30m)이하 암반에서, 열차 주행으로 부터 유발되는 진동 크기는 기껏 커도 기준치의 10분의 1 이하이다.

• Journal of Animal Science and Technology
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• 제63권5호
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• pp.1194-1203
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• 2021

Preparation of recipient stallions is critical step to produce donor spermatogonial stem cell (SSC) derived sperm using transplantation technique. This study was conducted to evaluate the effects of intravenous busulfan infusion on germ cell depletion, semen production, and libido in stallions. Six Thoroughbred stallions were separated into two treatment groups: 1) a multiple low-dose (2.5 mg/kg bw for the first 4 weeks and 5 mg/kg bw for the 5th week); and 2) control group treated with PBS. Testicular samples were obtained at 11 weeks and classified into three different patterns of spermatogenesis, such as normal, Sertoli cell only, and destroyed. Semen collection and libido experiments were performed 1 week before treatment, and 4 and 8 weeks after treatment. For the sperm analysis, total spermatozoa and motility were measured using a light microscope with a motility analyzing system. In the multiple low-dose group, the numbers of tubules categorized as Sertoli cell only were significantly higher than those in the control as well as the total population and total/progressive motility of sperm were significantly decreased 8 weeks after the start of the treatment. The sperm production and motility in the multiple low-dose group appears to be reduced, while libido was maintained. In conclusion, multiple administration of 2.5 mg/kg bw busulfan depletes endogenous germ cells in the stallion recipients for SSC transplantation.

• Reproductive and Developmental Biology
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• 제35권1호
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• pp.67-75
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• 2011

The faulty regulation of imprinting gene lead to the abnormal development of reconstructed embryo after nuclear transfer. However, the correlation between the imprinting status of donor cell and preimplantation stage of embryo development is not yet clear. In this study, to determine this correlation, we used the porcine spermatogonial stem cell (pSSC) and fetal fibroblast (pFF) as donor cells. As the results, the isolated cells with laminin matrix selection strongly expressed the GFR ${\alpha}$-1 and PLZF genes of SSCs specific markers. The pSSCs were maintained to 12 passages and positive for the pluripotent marker including OCT4, SSEA1 and NANOG. The methylation analysis of H19 DMR of pSSCs revealed that the zinc finger protein binding sites CTCF3 of H19 DMRs displayed an androgenic imprinting pattern (92.7%). Also, to investigate the reprogramming potential of pSSCs as donor cell, we compared the development rate and methylation status of H19 gene between the reconstructed embryos from pFF and pSSC. This result showed no significant differences of the development rate between the pFFs ($11.2{\pm}0.8%$) and SSCs ($13.3{\pm}1.1%$). However, interestingly, while the CTCF3 methylation status of pFF-NT blastocyst was decreased (36.3%), and the CTCF3 methylation status of pSSC-NT blastocyst was maintained. Therefore, this result suggested that the genomic imprinting status of pSSCs is more effective than that of normal somatic cells for the normal development because the maintenance of imprinting pattern is very important in early embryo stage.

• 한국항행학회논문지
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• 제12권2호
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• pp.139-145
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• 2008

최근 60GHz 대역의 millimeter wave 를 이용하는 통신 시스템에 관한 연구가 활발히 진행되고 있으며 새롭게 정의된 57~64GHz의 ISM 대역에 대한 관심이 집중되고 있다. 국내를 비롯한 미국, 일본, 유럽 등에서도 millimeter wave 를 이용하는 근거리 통신에 대한 연구가 진행 중이다. 그 중 IEEE 802.15.3c 표준화는 근거리에서 높은 전송 속도를 지원하기 위해 OFDM (Orthogonal Frequency Division Multiplexing) 방식의 시스템을 제안하고 있다. 하지만 OFDM 방식은 PAPR (Peak-to-Average Power Ratio) 이 발생하는 문제점을 가지고 있으며, PAPR 문제는 신호가 A/DC와 D/AC 그리고 전력 증폭기와 같은 비선형 소자를 통과할 때 심각한 왜곡을 발생시켜 전송 시스템의 성능이 저하된다. 본 논문에서는 PAPR 문제를 해결하기 위하여 Sine Soft Clipping (SSC) 기법을 제안하고, SAW (Surface Acoustic Wave) Filter와 전력 증폭기를 적용하여 IEEE 802.15.3c OFDM WPAN 전송 시스템의 PAPR, CCDF, PSD, BER 성능을 분석하였다.