• Title/Summary/Keyword: SPOT 4

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Electrophoretic Analysis on the Protein Alteration in the Brain of Actylamide Administered Mouse (Acrylamide 에 의한 생쥐 뇌단백질의 변화양상에 관한 전기영동적 분석)

  • 김동수;하재청
    • The Korean Journal of Zoology
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    • v.33 no.4
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    • pp.461-467
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    • 1990
  • To investigate the neurological effect of acrylamide, whole brain of Intoxicated mouse induced early hindlimbs ataxia was examined by using the methods of SDS-PAGE and two-dimensional electrophoresis. In the gel patterns by SDS-PAGE, when the patterns of each group were compared relatively, there were no remakable changes but in the patterns of 2D-PAGE, some protein alterations were observed. Especially, the spots containing 20 (14,500, 5.64) and 21 (19,900, 6.78) were disappeared, and the spots 9 (31,300, 5.82), 11 (31,300, 5.36) and 19 (16,400, 5.42) showed marked decrease relatively in the case of treatment group. Among these changed spots, the spot 20 (14,500, 5.64) showed higher quantity than that of control group but several spots containing the spots 11 (31,300, 5.36), and 19(16.400, 5.42) were identical or equal to those of control In quantity in the case of recovery group. It seems that acrylamide might already inhibit the brain protein synthesis mechanism at the time of onset of distal neuropathy by participation in the protein metabolism so as to impair the brain regulation ability followed by a malfunction of mouse central nervous system (CNS) and recovery is gradually progressed with the dose and duration dependence after the cessation of acrylamide administration.

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Synergistic Interaction of Fungicides in Mixtures under Different Conditions of Dollar Spot Disease Caused by Sclerotinia homoeocarpa (잔디 동전마름병의 발생정도가 다른 골프장 그린 조건에서 살균제 혼용살포에 의한 상승적 방제 효과)

  • Chang, Seog-Won;Jung, Suk-Woo;Kim, Se-Hun;Park, Jong-Hyun;Lee, Ji-Young
    • Asian Journal of Turfgrass Science
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    • v.26 no.2
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    • pp.96-101
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    • 2012
  • Dollar spot caused by Sclerotinia homoeocarpa F.T. Benn. is a common and economically important turfgrass disease in South Korea. Fungicides were evaluated for control of dollar spot in creeping bentgrass golf course putting green. Commercial formulations of propiconazole, thiophanate-methyl, chlorothalonil, trifloxystrobin and boscalid were applied to plots of creeping bentgrass, each of the latter four fungicides was tank mixed with propiconazole at the same rates, and applied as treatments. The dollar spot severity in the nontreated plots of field A and B progressed toward peak diseases of 18.3 and 66.7% from 10 or 15 days after inoculation, respectively. Significant differences were detected among control values of the fungicides. Dollar spot control provided by boscalid was significantly greater than the other fungicides which showed low control values in a higher disease condition. No synergistic interactions, except propiconazole + thiophanate-methyl treated plot, were detected under a lower disease pressure. However, under a higher disease pressure, synergism was observed at all fungicide combinations, except a propiconazole and boscalid tank mixture. These data suggest turfgrass managers in golf course can take advantage of fungicide synergism to control dollar spot using the products and rates in this study.

A Study on the Efficiency of KTB Forward Markets (국채선도금리(Forward rate)의 효율성(Efficiency)에 관한 연구)

  • Moon, Gyu-Hyun;Hong, Chung-Hyo
    • The Korean Journal of Financial Management
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    • v.22 no.2
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    • pp.189-212
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    • 2005
  • This study examines the interactions between KTB spot and futures markets using the daily prices from March 4, 2002 to January 31, 2005. We use Granger causality test, impulse Response Analysis and Variance Decomposition through vector autoregressive analysis (VAR). However, considering the long-term relationships between the level variables of KTB spot and futures, we introduced Vector Error Correction Model. The main results are as follows. According to the results of Granger-causality test and impulse response analysis, we find that the yields of KTB forward have a great influence on the change of KTB spot but not vice versa. In terms of volatility analysis, there is no inter-dependence between KTB forward and spot markets. In the variance decomposition analysis we find that the short-term KTB forward has much more impact on the KTB spot market than the long-term KTB forward does. We think these results are meaningful for bond investors who are in charge of capital asset pricing valuation, risk management and international portfolio management.

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Temperature Characteristic Analysis according to Variation of Properties of Transformer Insulating Oil (변압기 절연유의 물성치 변화에 따른 온도특성해석)

  • Kim, Ji-Ho;Rhee, Wook
    • The Transactions of the Korean Institute of Electrical Engineers P
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    • v.63 no.4
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    • pp.327-332
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    • 2014
  • In this paper, the temperature distribution according to the property change of the insulating oil of the power transformer and max temperature were predicted through the ductility interpretation which heat-flow is coupled. By using CFD (Computation Fluid Dynamics) for the interpretation, the temperature distribution of 154kV the class single phase power transformer was predicted. The power loss causing the temperature rise of the transformer was changed to the heat source and we used as the input value for the heat-flow analysis. The temperature distribution was predicted according to the change of the density, specific heat, thermal conductivity and viscosity, that is the ingredient having an effect on the temperature rise of the transformer oil. The mineral oil of 4 kinds used in domestic and international based on the interpreted result was selected and the temperature distribution according to each load and Hot Spot temperature was predicted.

AN ARTIFICIAL NEURAL NETWORK BASED SENSOR SYSTEMS FOR GAS LEAKAGE MONITORING

  • Ahn, Hyung-Il;Kim, Eung-Sik;Lee, June-Ho
    • Proceedings of the Korea Institute of Fire Science and Engineering Conference
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    • 1997.11a
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    • pp.282-288
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    • 1997
  • The purpose of this paper is to predict the situation of leak in closed space using an Artificial Neural Network (ANN). The existing system can't monitor the whole He situations with on/off signals. Especially the first stage of data determines the leak spot and intensity is disregarded in gas accidents. To complement these faults, a new prototype of monitoring system is proposed. Ihe system is composed of'sensing systenL data acquisition system computer, and ANN implemented in software and is capable of identifying the leak spot and intensity in closed space. The concentration of gas is measured at the 4 different places. The network has 3 layers that are composed of 4 input Processing Element (PE),24 hidden PEs, md 4 output PEs. The ANN has optimum condition through several experiments and as a consequence the recognition rate of93.75% is achieved finally

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A Study on the Visual Preference of Users according to the Location of Benches at Urban Community Parks (도시공원에서 벤치의 배치장소에 따른 이용자의 시각적 선호도에 관한 연구)

  • 유상완;문석기;권상준
    • Archives of design research
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    • v.13 no.2
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    • pp.95-102
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    • 2000
  • The purpose of this study is to find out what is the preference of users according to the location of benches at urban community parks. This location of benches is seperated into 4 patterns according to arranging pattern of water space, a walk, pergola and shelter, greenspace. To investigate the visual preference is examined by analyzing visual volume of 4 patterns. Results are as follows; 1. Factor analysis by the total data showed that 5 factors explain 60.40 percent of total variance of the location of bench visual character. They were classified by the sensitive factor, visual factor, physical-individual factor, distinct factor, density factor. Among 5 factors, the sensitive factor which represented psychological reaction was appreciated to be highest. 2. Most of 20 items showed the following scores of mean values in sementic differential experiment : Spot 1->Spot 4-> 2-> 3. The mean values between arrangement place locational differences showed significantly, that could explain to be a violent contrast between the natural factors(weater space, green space, etc) and the artificial factors (around of pergola, shelter, etc)

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Studies on Mulberry Shoot Rot caused by Fusarium spp. (Fusarium spp. 균에 의한 뽕나무신소썩음병에 관한 연구)

  • 윤형주;김영택;진경식;박인균;양성열
    • Journal of Sericultural and Entomological Science
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    • v.37 no.1
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    • pp.86-91
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    • 1995
  • Isolation and pathogenicity of Fusarium spp. from mulberry shoot rot and severity of diseases which were known as bacterial blight were examined on four mulberry varieties in Suwon, Kongju and Chuncheon, A symptom of mulberry shoot rot was initiated long brown spot on young leaves and shoots. It was developed into dark brown spot and produced white mycelia and spores on the diseased symptoms. A symptom of bacterial blight showed leaf rolling and water soaking spot and produced bacterial ooze on leaf and shoot However later stage of upper two types of symptom was hardly distinguished. Severities of shoot rot and bacterial blight were 7.5% and 4.4% in Suwon, respectively. Isolation of Fusarium spp. on shoot rot symptoms was highter than that on bacterial blight symptoms, but isolation of Pseudomonas spp. was higher on bacterial blight symptoms. Trends of pathogenicity of Fusarium spp. and Pseudomonas spp. were similar to inoculation works, and isolations of pathogenic Fusarium spp. from center of symptom was higher than that from 30cm of symptom of all samples in three cultivation areas. Disease severities of shoot rot on variety of Kaeryangppong were 13.9%, 15.9% and 17.2% in Suwon, Kongju and Chuncheon, respectively. However variety of Cheongolppong was highly resistant to shoot rot disease in three cultivation areas.

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Cold Shock Response of Leuconostoc mesenteroides SY1 Isolated from Kimchi

  • KIM JONG HWAN;PARK JAE-YONG;JEONG SEON-JU;CHUN JIYEON;KIM JEONG HWAN
    • Journal of Microbiology and Biotechnology
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    • v.15 no.4
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    • pp.831-837
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    • 2005
  • Low-temperature adaptation and cryoprotection were studied in Leuconostoc mesenteroides SYl, a strain isolated from Kimchi. L. mesenteroides SY1 cells grown in exponential growth phase at $30^{\circ}C$ were exposed to $15^{\circ}C,\;10^{\circ}C$, and $5^{\circ}C$ for 2, 4, and 6 h, respectively, and then frozen at $- 70^{\circ}C$ for 24 h. Survival ratio was measured after the cells were thawed. The freezing-thawing cycles were repeated four times. Preadapted cells survived better than non-adapted control cells, and the highest survival ratio ($96\%$) was observed for cells preadapted for 2 h at $5^{\circ}C$, whereas control cells showed only $22\%$. The 2D gel showed that two proteins (spots A and B) were induced in cells preadapted at lower temperatures. Spots A and B have the same molecular weight (7 kDa), but the pI was 4.6 for spot A and 4.3 for spot B. The first 29 and 15 amino acid sequences from spots A and B were determined, and they were identical, except for one amino acid. A csp gene was cloned, and nucleotide sequencing confirmed that the gene encoded spot A cold shock protein.

Incidence Rates of Major Diseases on Green-Fleshed Kiwifruit cv. Hayward and Yellow-Fleshed Kiwifruit cv. Haegeum (그린키위 품종 헤이워드와 골드키위 품종 해금의 주요 병 발병률)

  • Kim, Gyoung Hee;Koh, Young Jin
    • Research in Plant Disease
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    • v.24 no.3
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    • pp.175-181
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    • 2018
  • Incidence rates of bacterial canker, bacterial leaf spot and postharvest fruit rot on the Korean yellow-fleshed kiwifruit cv. Haegeum were compared with those on the most popular green-fleshed kiwifruit cv. Hayward grown in several naturally infected kiwifruit orchards in 2013 and 2014. The percentages of diseased leaves caused by bacterial canker were 18.5% and 17.3% on Hayward in 2013 and 2014, but those on Haegeum were 1.2% and 0%, respectively. The percentages of diseased leaves caused by bacterial leaf spot on Hayward were 63.5% and 16.2% in 2013 and 2014, respectively, but no bacterial leaf spots were observed on Haegeum in both years. The average percentages of diseased fruits caused by postharvest fruit rot were 24.2% and 20.5% on Hayward in 2013 and 2014, while 6.3% and 4.4% and Haegeum, respectively. Botryosphaeria dothidea was turned out to be the major pathogen of postharvest fruit rot on both cultivars.

Preparation of Oligosaccharides from Alginic Acid by Enzymic Hydrolysis (효소분해에 의한 알긴산 올리고당류의 제조)

  • Joo, Dong-Sik;Lee, Jung-Suck;Park, Jung-Je;Cho, Soon-Yeong;Kim, Hee-Kyung;Lee, Eung-Ho
    • Korean Journal of Food Science and Technology
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    • v.28 no.1
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    • pp.146-151
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    • 1996
  • For the purpose of production of oligosaccharides from alginates, a bacterium was isolated from seaweed, and then an enzyme which degraded alginates was obtained from the bacterium. A specific activity of the enzyme was shown in G-rich block and Na-alginate (Wako Co.) as a result of reaction between the enzyme and six types of alginates (G-rich block, M-rich block and 4 commercial Na-alginate). Degradation products were prepared from the Na-alginate (Wako Co.) by the enzyme. The oligosaccharides were fractioned by Sephadex G-25 and Bio-gel P-2 and identified on a thin layer chromatography (TLC). Degree of polymerization (DP) of the oligosaccharides was shown from 2.6 to 7.5.

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