Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
권호 표지

Preparation of Oligosaccharides from Alginic Acid by Enzymic Hydrolysis

효소분해에 의한 알긴산 올리고당류의 제조

  • Joo, Dong-Sik (Department of Food Science and Technology, National Fisheries University of Pusan) ;
  • Lee, Jung-Suck (Department of Food Science and Technology, National Fisheries University of Pusan) ;
  • Park, Jung-Je (Department of Food Science and Technology, National Fisheries University of Pusan) ;
  • Cho, Soon-Yeong (Department of Food Science, Kangnung National University) ;
  • Kim, Hee-Kyung (Department of Food Science and Technology, National Fisheries University of Pusan) ;
  • Lee, Eung-Ho (Department of Food Science and Technology, National Fisheries University of Pusan)
  • 주동식 (부산수산대학교 식품공학과) ;
  • 이정석 (부산수산대학교 식품공학과) ;
  • 박중제 (부산수산대학교 식품공학과) ;
  • 조순영 (강릉대학교 식품과학과) ;
  • 김희경 (부산수산대학교 식품공학과) ;
  • 이응호 (부산수산대학교 식품공학과)
  • Published : 1996.02.28
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Abstract

For the purpose of production of oligosaccharides from alginates, a bacterium was isolated from seaweed, and then an enzyme which degraded alginates was obtained from the bacterium. A specific activity of the enzyme was shown in G-rich block and Na-alginate (Wako Co.) as a result of reaction between the enzyme and six types of alginates (G-rich block, M-rich block and 4 commercial Na-alginate). Degradation products were prepared from the Na-alginate (Wako Co.) by the enzyme. The oligosaccharides were fractioned by Sephadex G-25 and Bio-gel P-2 and identified on a thin layer chromatography (TLC). Degree of polymerization (DP) of the oligosaccharides was shown from 2.6 to 7.5.

6종류의 알긴산을 기질로하여 효소 반응을 시켜 알긴산 올리고당 제조를 시도한 결과, 실험에 사용한 효소의 경우 G-rich block에만 특이하게 작용하는 guluronate lyase 일종으로 G-block에 주로 작용하여 올리고당을 생성하는 것으로 확인되었다. 또한 4종류의 시판 알긴산중에서도 1종(Wako Co.)의 Na-alginate에 대해서 특이하게 6-7개의 올리고당 spot가 TLC에서 확인되었고, 그외 3종의 Na-alginate는 2-4개 정도의 올리고당 spot가 TLC에 나타났다. 6-7개의 spot가 확인된 Na-alginate(Wako Co.)를 기질로 대량 분해시킨 후 Sephadex G-25 및 Bio-gel p-2로 분획, 정제를 행하고 중합도를 측정하여 올리고당을 확인한 결과, 중합도가 각각 다른 4개의 올리고당을 확인할 수 있었다.

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