• Korean Journal of Plant Taxonomy
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• v.42 no.4
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• pp.340-353
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• 2012

Records found on labels of specimens deposited at University of Tokyo herbarium (TI) and references about Japanese naturalist, Tamezo Mori (1884-1964) were assembled to produce collector's itineraries from 1909 to 1916 in Korea. Mori collected large numbers of botanical specimens from different parts of the country. In August 1911 and 1912, Mori conducted botanical collections in Jeju and southern parts of Korean Peninsula. In 1913 major collections in North Korea by Mori took place in Mt. Baekdusan, Hamkyongnam-do. Following this exploration, in August 1916 Mori conducted another major collections in Pyeongannam-do and Hamgyeongnam-do. It is estimated that approximately 966 Korean specimens collected by Mori are currently held within the collections of The University of Tokyo herbarium (TI). Significantly these holdings also contain 46 type specimens (11 holotypes, 23 syntypes and 12 paratypes).

• Journal of Plant Biotechnology
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• v.5 no.4
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• pp.215-219
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• 2003

Optimal shoot regeneration and transformation conditions of root type chicory (Cichorium intybus L. var. sativus cv Cesare) were studied. Leaf explants were co-cultured with Agrobacterium tumefaciens, which contained NPTII as a selectable marker and a rice homeotic gene, OsMADS1, that encodes a MADS-domain-containing transcription factor. After one day of co-cultivation, explants were transferred to selection media consisting of MS basal medium supplemented with 0.5 mg/L BAP, 0.1 mg/L IAA, 70 mg/L kanamycin, and 250 mg/L cefotaxime. PCR and Southern blot analyses revealed stable integration of the OsMADS1 gene in the chicory genome. Four-teen original transgenic plants ($T_o$ plants) were acclimatized in the greenhouse and examined for their morphological characters. Most of the transgenic plants showed altered morphologies, such as short, bushy, and early-flowering phenotypes with reduced apical dominance. Additionally, half of the transgenic plants exhibited altered leaf shapes, and 4 out of 14 plants were sterile. These phenotypes were inherited by the next generation. Northern blot analysis confirmed expression of the OsMADS1 gene in both floral and vegetative organs.

• Journal of Plant Biotechnology
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• v.38 no.4
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• pp.272-277
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• 2011

Plants expressing Agrobacterium sp. strain CP4 5-enolpyruvylshikimate-3-phosphate synthase (CP4 EPSPS) are known to be resistant to glyphosate, a potent herbicide that inhibits the activity of the endogenous plant EPSPS. In order to develop herbicide-resistant rice, we prepared transgenic rice plants with CP4 EPSPS gene under the control of CaMV 35S promoter for over-expression. A recombinant plasmid was transformed into rice via Agrobacterium-mediated transformation. A large number of transgenic rice plants were obtained with glyphosate and most of the transformants showed fertile. The integration and expression of CP4 EPSPS gene from regenerated plants was analyzed by Southern and northern blot analysis. The transgenic rice plants had CP4 EPSPS enzyme activity levels more than 15-fold higher than the wild-type plants. EPSPS enzyme activity of transgenic rice plants was also identified by strip-test method. Field trial of transgenic rice plants further confirmed that they can be selectively survived at 100% by spay of glyphosate (Roundup$^{(R)}$) at a regular dose used for conventional rice weed control.

• Korean Journal of Plant Resources
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• v.29 no.4
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• pp.504-510
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• 2016

This study was investigated to find the effects of sowing time on growth and yields of cowpea grown in a southern region of South Korea. Experiments were carried out in Naju, Jeonnam Province (Latitude 35° 04' N, Longitude 126° 54' E) during 2013 and 2014. The intermediate-erect type strains used in this study were Jeonnam1 and Jeonnam2 (Okdang). Sowing time was performed between middle-April and middle-August at intervals of one month. The days from sowing to emergence was significantly higher in the middle-April sowing (12 days) time but there was no significant differences other sowing dates (5 to 3 days). The days from sowing to first flowering were shorter for sowing dates between middle-April and middle-July because sowing time was delayed and then were lengthened again at middle-August sowing; the days were longest at middle-April sowing (around 62 days) and were shortest at middle-July sowing (35 days). The days from first flowering to harvesting were short for the sowing dates between middle-April and middle-July (17 to 15 days) but were relatively long for middle-August sowing date (24 days). Stem length was relatively long for the middle-May and middle-June sowing dates. Peduncle length was relatively long for the middle-April sowing date. Main-stem node number was highest for the middle-July sowing. Branch number per plant was highest in the middle-April sowing. Seed yields were highest for the middle-April sown Jeonnam1 and the Jeonnam2 (Okdang) strains showing, 199 and 211 kg/10a, respectively, and then followed by sowing in middle-July, 191 and 195 kg/10a, respectively.

• Korean Journal of Plant Resources
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• v.31 no.5
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• pp.531-537
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• 2018

This study was carried to survey distribution of the nucleotide polymorphisms in heme-binding (HB) domain, which is highly conserved region between 1,210 and 1,240 bp of cytochrome P450, in domestic garlic cultivars. 120 garlic cultivars collected from Korea were classified into seven HB domain variation based on the nucleotide sequence of the domain. Northern type garlic cultivars, collected from Kyungpook, Chungnam, Chungpook and Kangwon province, showed 51.3% of KP2 type nucleotide sequence, 5'-TTT/GGC/GGT/GGA/CGG/AGA/ATA/TGT/CCT/GGA-3' with coding amino acid FGGGRRICPG, 13.7% of KP1, 11.3% of CP, 8.8% of CM and 5% of KW2 types. Southern type cultivars, collected from Kyungnam province, showed 52.5% of KM type nucleotide sequence, 5'-TTT/GGC/GCA/GGA/CGG/AGA/ATT/TGT/CCT/GGA-3' with coding amino acid FGAGRRICPG, 22.5% of KP2, 5.0% of KW2 and 2,5% of CP type nucleotide sequence. These results showed that Korean garlics were cultivated in highly mixed condition even in the same region.

• Journal of Plant Biotechnology
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• v.37 no.3
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• pp.313-318
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• 2010

To develop edible vaccines for swine, the embryogenic calli (type II) derived from HiII genotype were inoculated with A. tumefaciens strain C58C1 containing the binary vector pMYV611, 613, 616, and V621, 622 and 623 respectively. Six of those vectors carry nptII gene which confers resistance to paromomycin and apxIIA gene producing ApxII toxin which is generated in various serum types of A. pleuropneumoniae as a target gene. The 4,120 callus clones for pMYV611, 5,959 callus clones for pMYV613, 7,581 callus clones for pMYV616, 52,329 callus clones for V621, 48,948 callus clones for V622, and 56,188 callus clones for V623 were inoculated. The frequency of positive response clone was confirmed into range of 2.3% - 4.4% for each vectors by NPTII ELISA kit assay, and the selected callus clones of them were finally 3 callus clones from pMYV611 (0.07%), 4 callus clones from pMYV613 (0.07%), 2 callus clones from pMYV616 (0.03%), 51 callus clones from V621 (0.1%), 72 callus clones from V622 (0.15%), and 102 callus clones from V623 (0.18%) respectively. From the selected callus clones of each binary vector, the integration of the apxIIA gene into maize genome was detected from 2 plants of pMYV613 and 2 plants of V623 by Southern blot analysis.

• Journal of Korean Society of Forest Science
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• v.30 no.1
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• pp.50-60
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• 1976

For finding out the proper method of developing Buxus koreana var. insularis, this study was carried out in Pokil-Do, Wan-Do, Chonnam, in 1975. The ecology, morphology and characteristics of the plant were mainly investigated and analyzed. The results are summarized as follows; 1. The small islands of the southern sea of Korea; Pokil-Do, Chin-Do and So-Huk-San-Do, turn out to be the site of the natural communities of B. koreana var. insularis. 2. The growth of the plant is fairly good in the acid soil of these islands. 3. This vegetation of composed as the three-layered communities, these communities consit of Camellia, Buxus and Cares. 4. The plant is a board leaved evergreen shrub that is tolerant to shade and native to the temperate zone. 5. The size of the leaves, fruits and seeds is slightly larger than those of B. koreans. 6. As fruiting and germination of the seeds are favorable. it is easy for us to make a number of seedlings. 7. The proper times for sowing and harvesting the seeds are around the middle of July and August, respectively. 8. The seedlings is difficult to grow in the naked ground because the germinated seeds are seriously damaged by frost. 9. The plant growth per annum is about 8 to 10cm in height, 1.5mm in diameter. It is graceful looking as the branches and leaves as of the dense foliage type 10. It is possible to develop the plant in many ways, such as; ornamental plant, bee plant, sculptural and industrial materials.

• The Plant Pathology Journal
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• v.35 no.6
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• pp.644-653
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• 2019

The genetic variability of 'Candidatus Liberibacter asiaticus' (CLas) population associated with huanglongbing (HLB) disease of citrus in North Eastern (NE) region of India, a geographically locked region, and home for the diversity of many citrus species was analyzed on the basis of tandem repeat numbers (TRN) in variable CLIBASIA_01645 genomic loci. Fifty-five CLas strains sampled from different groves of NE Hill (NEH) region of India were in single amplicon group, but there was remarkable genetic variability in TRNs. The TRN in HLB-associated CLas strains varied from 0-21 and two novel repeat motifs were also identified. Among the NE population of CLas, TRN5 and TRN9 were most frequent (total frequency of 36.36%) followed by TRN4 (14.55%) and TRN6, TNR7 with a frequency of 12.73% each. Class II type CLas genotypes (5 < TRN ≤ 10) had highest prevalence (frequency of 60.00%) in the samples characterized in present study. Class I (TRN ≤ 5) genotypes were second highest prevalent (29.09%) in the NEH region. Further analysis of genetic diversity parameters using Nei's measure (H value) indicated wide genetic diversity in the CLas strains of NE India (H value of 0.58-0.86). Manipur CLas strains had highest genetic variability (0.86) as compared to Eastern, Southern and Central India. The R₁₀ values (TRN ≤ 10/TRN > 10) of NE CLas population was 10.43 (73/7), higher from other regions of India. Present study conclusively reported the occurrence of high genetic variability in TRN of CLas population in North East Indian citrus groves which have evolved to adapt to the specific ecological niche.

• Journal of Ecology and Environment
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• v.32 no.3
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• pp.183-189
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• 2009

This research focused on the effects of fragmentation on moth diversity in an agricultural land-scape by comparing moth species richness and abundance between hillocks and young secondary forests. We examined five sites from the southwestern part of South Korea: three sites from hillock forest and two from secondary forest. We collected moths bi-weekly from April to October for a 2-year period (2006-2007) with a UV light trap that usually attracts moths within 30 meters. Tree species richness and abundance in $20m{\times}20m$ plots at each moth sampling site showed a substantial difference in tree diversity between the two types of forest habitats. The total abundance and richness of moth species were higher in secondary forests (541 species with 4,998 individuals) than in hillock forests (423 species with 3,913 individuals), irrespective of the distance among the sites. An ordination analysis with NMDS showed that habitat is the most important factor of grouping sites. The food preferences of the dominant moth species i,n each habitat were closely related to the habitat type.

• Journal of Korean Academy of Rural Health Nursing
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• v.8 no.1
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• pp.33-41
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• 2013

Purpose: The study was done to explore relationships between residential areas and smoking rates and to identify related factors contributing to smoking in Korea adolescents. Methods: An analysis was done of smoking rates and socioeconomic position indicators by city size based on a 2012 cross-sectional nationwide online survey conducted with 74,186 Korean middle and high school students aged 12-18 years old. Data were analyzed using x2-test and multiple logistic regression with the SPSS/WIN18.0 program. Results: Analyses revealed that rural boys were more likely to be current smokers compared to metropolitan boys (odds ratio 1.18, 95%-confidence interval 1.01; 1.38) but residential areas and smoking rates among girls were not related. After adjusting for covariates, results showed that city size, Family affluence score, economic status, parents' education level, living with parents, school type, and school achievement were related to increased an proportion of adolescents who smoked. Conclusion: In conclusion, rural living is a determinant of smoking among boys. Tobacco control programs should recognize differences in living conditions between rural and urban areas.