• Molecules and Cells
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• 제43권4호
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• pp.313-322
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• 2020

Eukaryotes transport biomolecules between intracellular organelles and between cells and the environment via vesicle trafficking. Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNARE proteins) play pivotal roles in vesicle and membrane trafficking. These proteins are categorized as Qa, Qb, Qc, and R SNAREs and form a complex that induces vesicle fusion for targeting of vesicle cargos. As the core components of the SNARE complex, the SNAP25 Qbc SNAREs perform various functions related to cellular homeostasis. The Arabidopsis thaliana SNAP25 homolog AtSNAP33 interacts with Qa and R SNAREs and plays a key role in cytokinesis and in triggering innate immune responses. However, other Arabidopsis SNAP25 homologs, such as AtSNAP29 and AtSNAP30, are not well studied; this includes their localization, interactions, structures, and functions. Here, we discuss three biological functions of plant SNAP25 orthologs in the context of AtSNAP33 and highlight recent findings on SNAP25 orthologs in various plants. We propose future directions for determining the roles of the less well-characterized AtSNAP29 and AtSNAP30 proteins.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2022년도 추계학술대회
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• pp.88-88
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• 2022

In this study, we investigated the effect of Solanum nigrum aerial parts (SNAP) on macrophage activation and macrophage autophagy in RAW264.7 cells. SNAP increased the production of immunostimulatory factors and phagocytosis in RAW264.7 cells. TLR4 inhibition blocked SNAP-mediated production of immunostimulatory factors. In addition, the JNK inhibition reduced the SNAP-mediated production of immunostimulatory factors, and the SNAP-mediated JNK activation was blocked by the TLR4 inhibition. SNAP activated macrophage autophagy. TLR4 inhibition blocked SNAP-mediated macrophage autophagy and inhibition of p38 and JNK attenuated SNAP-mediated macrophage autophagy. These findings indicate that SNAP may induce TLR4/JNK-mediated macrophage activation and TLR4/p38 and JNK-mediated macrophage autophagy.

• 한국멀티미디어학회논문지
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• 제17권6호
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• pp.706-715
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• 2014

It is used to lead to serious structural vulnerability of the system security of security-critical system when we have quickly developed software system according to urgent release schedule without appropriate security planning, management, and assurance processes. The Data Set and Provider of DataSnap, which is a middleware of Delphi XE2 of the Embarcadero Technologies Co., certainly help to develop an easy and fast-paced procedure, but it is difficult to apply security program and vulnerable to control software system security when the connection structure Database-DataSnap server-SQL Connection-SQL Data set-Provider is applied. This is due to that all kinds of information of Provider are exposed on the moment when DataSnap Server Port is sure to malicious attackers. This exposure becomes a window capable of running SQL Command. Thus, it should not be used Data Set and Provider in the DataSnap Server in consideration of all aspects of security management. In this paper, we study on the verification of the security vulnerabilities for Client and Server DataSnap in Dlephi XE2, and we propose a secure coding method to improve security vulnerability in the DataSnap server system.

• 한국수정란이식학회지
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• 제33권4호
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• pp.211-220
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• 2018

Nitric oxide (NO) has an important role in oocyte maturation and embryonic development in mammals. This study examined the effect of exogenous NO donor S-nitroso-N-acetylpenicillamine (SNAP) in a maturation medium on meiotic progression and embryonic development after parthenogenesis (PA) and somatic cell nuclear transfer (SCNT) in pigs. When oocytes were exposed to $0.1{\mu}M$ SNAP for first 22 h of in vitro maturation (IVM) in Experiment 1, SNAP significantly improved blastocyst development in both defined and standard follicular fluid-supplemented media compared to untreated control (48.4 vs. 31.7-42.5%). SNAP treatment significantly arrested meiotic progression of oocytes at the germinal vesicle stage at 11 h of IVM (61.2 vs. 38.7%). However, there was no effect on meiotic progression at 22 h of IVM (Experiment 2). In Experiment 3, when oocytes were treated with SNAP at 0.001, 0.1 and $10{\mu}M$ during the first 22 h of IVM to determine a suitable concentration, $0.1{\mu}M$ SNAP (54.2%) exhibited a higher blastocyst formation than 0 and $10{\mu}M$ SNAP (36.6 and 36.6%, respectively). Time-dependent effect of SNAP treatment was evaluated in Experiment 4. It was observed that SNAP treatment for the first 22 h of IVM significantly increased blastocyst formation compared to no treatment (57.1% vs. 46.2%). Antioxidant effect of SNAP was compared with that of cysteine. SNAP treatment significantly improved embryonic development to the blastocyst stage (49.1-51.5% vs. 34.4-37.5%) irrespective of the presence or absence of cysteine (Experiment 5). Moreover, SNAP significantly increased glutathione (GSH) content and inversely decreased the reactive oxygen species (ROS) level and mitochondrial oxidative activity in IVM oocytes. SNAP treatment during IVM showed a stimulating effect on in vitro development of SCNT embryos (Experiment 7). These results demonstrates that SNAP improves developmental competence of PA and SCNT embryos probably by maintaining the redox homeostasis through increasing GSH content and mitochondrial quality and decreasing ROS in IVM oocytes.

• 한국조리학회지
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• 제17권2호
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• pp.198-208
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• 2011

본 연구는 강황분말의 첨가량(0, 3, 6, 9, 12, 15%)을 달리 한 sugar-snap cookies의 품질 특성과 관능특성을 평가한 것으로 강황분말을 첨가한 sugar-snap cookies 반죽의 pH는 6.58~7.32 범위였으며 sugar-snap cookies의 수분함량은 9.09~10.77% 사이의 범위였다. 퍼점성은 유의적으로 낮아지는 경향을 보였으며, 강황분말 첨가량이 증가할수록 쿠키의 명도는 감소하고 a값과 b 값은 유의적으로 증가하는 경향이 나타났다(p<0.05). 경도(hardness)는 강황분말 첨가량이 증가할수록 증가하였으며 관능검사는 맛, 색, 향, 조직감, 전체적인 기호도의 항목에서 강황분말 첨가향이 6%일 때 전반적으로 가장 높게 평가되어, 6%의 강황분말을 첨가한 sugar-snap cookies 제조가 적합하다고 사료된다.

• 대한의생명과학회지
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• 제6권1호
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• pp.11-17
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• 2000

SNAP-25는 presynaptic plasma membrane에 위치하는 단백질로서 synaptic vesicle의 docking과 fusion에 있어서 매우 중요한 역할을 한다. 생쥐 SNAP-25$^{2)}$ 유전자와 99%의 높은 homology를 갖고 있는 Z2 cDNA를 probe로 사용하여 쥐의 뇌 cDNA library에서 SNAP-25유전자를 screening하였다. 그 결과 6개 의 양성 클론을 분리 해 냈으며, 이들 각각을 S1, S2, S3, S4, S5, S6으로 명명하였다. 이 중에서 생쥐 SNAP-25와 가장 높은 homology를 보여 주고 있는 S5 클론을 선택하여 염기서열을 분석하였다. 2,100 bp의 염기서열로 구성된 쥐 SNAP-25 cDNA는 206개의 아미노산을 coding하는 618 bp의 open reading frame을 가지고 있으며, ORF는 209~211 bp에 위치하는 AUG codon에서 시작하여 827~829 bp에 위치하는 stop codon TAA에서 끝난다. 3' untranslated region에서 는 28과 19개 의 CA 반복 염기서열을 보여주고 있었으며, SNAP-25 peptide sequence에서 4개의 cystein residues는 84~91에 위치하고 있었으며, amino terminus 부분에서 amphipathic $\alpha$-helix를 형성하고 있는 것을 볼 수 있었다. 사람과 쥐의 SNAP-25 유전자는 88%, 생쥐와 쥐의 경우는 97%의 homology를 보여 주고 있었다. 그리고 사람과 쥐의 ORF에서 염기서열은 94%,생쥐와 쥐의 ORF에서 염기서열은 100%의 homology를 보여주고 있었으며 사람, 생쥐, 그리고 쥐의 ORF에서 아미노산 서열은 100%의 homology를 보여주고 있었다.

• 한국공작기계학회논문집
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• 제15권6호
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• pp.44-49
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• 2006

The use of snap-fit features is highly recommended to reduce overall product cost and manufacturing time by reducing the number of parts and eliminating assembly with conventional fasteners. Application to particular product for integral attachment using snap-fit features is needed. Alternative attachment concept for specific application has been introduced. In this study, the optimal attachment design based on the given design objectives is identified using newly developed alternative attachment concept for the specific application to LCD monitor case assembly. Integral attachment using snap-fit features applies the systematic procedure and the intensity analysis.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 2007년도 정기 학술대회 논문집
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• pp.539-544
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• 2007

Glass fiber reinforced composite decks have high-strength, light-weight and high durability. The composite decks having vertical snap-fit connections are designed for pedestrian bridges and their structural behaviour are studied. The existing connection method of the composite decks in horizontal direction is replaced by the developed snap-fit connection method in vertical direction. The section shape of the composite decks having the vertical snap-fit connection is designed. The safety of the vertical snap-fit connection is verified by finite element analysis.

• Journal of Mechanical Science and Technology
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• 제17권1호
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• pp.85-96
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• 2003

In the present investigation, the nonlinear dynamic buckling of a curved plate subjected to sinusoidal loading is examined. By the theoretical analyses, a highly nonlinear snap-through motion of a clamped-free-clamped-free plate and its effect on the overall vibration response are investigated. The problem is reduced to that of a single degree of freedom system with the Rayleigh-Ritz procedure. The resulting nonlinear governing equation is solved using Runge-Kutta (RK-4) numerical integration method. The snap-through boundaries, which vary with different damping coefficient and linear circular frequency of the flat plate are studied and given in terms of force and displacement. The relationships between static and dynamic responses at the start of a snap-through motion are also predicted. The analysis brings out various characteristic features of the phenomenon, i.e. 1) small oscillation about the buckled position-softening spring type motion, 2) chaotic motion of intermittent snap-through, and 3) large oscillation of continuous snap-through motion crossing the two buckled positions-hardening spring type. The responses of buckled plate were found to be greatly affected by the snap-through motion. Therefore, better understanding of the snap-through motion is needed to predict the full dynamic response of a curved plate.

• 한국식품과학회지
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• 제40권2호
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• pp.234-237
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• 2008

밀가루에 흑미가루를 첨가하여 제조한 sugar-snap 쿠키의 품질특성을 조사하였다. Sugar-snap 쿠키의 반죽의 pH는 흑미 가루의 첨가량이 증가함에 따라 감소하였다. 쿠키의 직경과 퍼짐성은 흑미 가루의 첨가량이 증가함에 따라 증가하였고 두께와 파괴강도는 감소하였다. L, a, 및 b값 역시 흑미 가루 첨가량이 증가함에 따라서 다소 감소하는 경향을 나타내었다. Sugar-snap 쿠키의 종합적 기호도는 강력분의 경우는 흑미가루 20% 첨가 시, 중력분은 25% 첨가 시, 박력분은 20% 첨가 시에 가장 좋은 것으로 평가되었다.