Acknowledgement
This work was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2018R1A6A1A03024862).
Proceedings of the Plant Resources Society of Korea Conference (한국자원식물학회:학술대회논문집)
- 2022.09a
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- Pages.88-88
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- 2022
Immunostimulatory Activity of Solanum nigrum Through TLR4-Mediated JNK Activation in RAW264.7 Cells
- Ju-Hyeong Yu (Department of Medicinal Plant Resources, Andong National University) ;
- So Jeong Park (Department of Medicinal Plant Resources, Andong National University) ;
- Jae Won Lee (Department of Medicinal Plant Resources, Andong National University) ;
- Jin Boo Jeong (Department of Medicinal Plant Resources, Andong National University)
- Published : 2022.09.27
Abstract
In this study, we investigated the effect of Solanum nigrum aerial parts (SNAP) on macrophage activation and macrophage autophagy in RAW264.7 cells. SNAP increased the production of immunostimulatory factors and phagocytosis in RAW264.7 cells. TLR4 inhibition blocked SNAP-mediated production of immunostimulatory factors. In addition, the JNK inhibition reduced the SNAP-mediated production of immunostimulatory factors, and the SNAP-mediated JNK activation was blocked by the TLR4 inhibition. SNAP activated macrophage autophagy. TLR4 inhibition blocked SNAP-mediated macrophage autophagy and inhibition of p38 and JNK attenuated SNAP-mediated macrophage autophagy. These findings indicate that SNAP may induce TLR4/JNK-mediated macrophage activation and TLR4/p38 and JNK-mediated macrophage autophagy.
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