• Title/Summary/Keyword: SMF

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Utilizing chromosome segment substitution lines (CSSLs) to evaluate developmental plasticity of root systems in hardpan penetration and deep rooting triggered by soil moisture fluctuations in rice

  • Nguyen, Thi Ngoc Dinh;Suralta, Roel R.;Mana, Kano-Nakata;Mitsuya, Shiro;Stella, Owusu Nketia;Kabuki, Takuya;Yamauchi, Akira
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.321-321
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    • 2017
  • Water availability in rainfed lowlands (RFL) is strongly affected by climate change. In RFL, rice plants are exposed to soil moisture fluctuations (SMF) but rarely to simple progressive drought as widely believed. Typical RFL field is characterized by a about 5-cm thick high bulk density hardpan layer underneath the cultivated layer at about 20 cm depth that impedes deep root development. Root system has the ability to develop in response to changes in SMF, known as phenotypic plasticity. We hypothesized that genotypes that can adapt to RFL have root plasticity. The roots can sharply respond to re-wetting after drought period and thus penetrate the hardpan layer when the hardpan is wet and so becomes relatively soft, and thus access water under the hardpan. This study aimed to identify CSSLs derived from a cross between Sasanishiki and Habataki which adapted to such RFL conditions. We used 39 CSSLs together with the parent Sasanishiki, which were grown in hydroponics and pot under transient soil moisture stresses (drought and then rewatering), and compared with continuously well-watered (WW) (control) up to 14 days after sowing (DAS), and 20 DAS, respectively. Based on the results of hydroponics and pot experiments, we selected a few lines, which were grown in the soil-filled rootbox with artificial hardpan layer and without artificial hardpan. For the rootbox without artificial hardpan, plants were grown under WW and transient soil moisture stresses for 49 DAS. While the rootbox with artificial hardpan, the plants were grown under WW (control) and SMF (WW up to 21 DAS, 1st drought (22-36 DAS), rewatering (37-44 DAS), and followed by 2nd drought (45-58 DAS)). Among the 39 CSSLs, only CSSL439 (SL39) consistently showed significantly higher shoot dry weight (SDW) than Sasanishiki under transient soil moisture stress conditions as well as SMF conditions in all the experiments. Furthermore, under WW, SL39 consistently showed no significant differences from Sasanishiki in shoot and root growth in most of traits examined. SL39 showed significantly greater total root length (TRL) than Sasanishiki under transient soil moisture stress, which is considered as phenotypic plasticity in response to rewatering after drought period. Such plastic root development was the key trait that effectively contributed to root elongation and branching during the rewatering period and consequently enhanced the root to penetrate hardpan layer when the soil penetration resistance at hardpan layer reduced. In addition, using the rootbox with artificial hardpan layer ($1.7g\;cm^{-3}$, heavily compacted), SL39 showed greater root system development than Sasanishiki under SMF, which was expressed in its significantly higher TRL, total nodal RL, and total lateral RL at hardpan layer as well as at below the hardpan layer. These results prove that SL39 has plasticity that enables its root systems to penetrate hardpan layer in response to rewatering. Under SMF, such root plasticity contributed to its higher gs and Pn.

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Isolation and Identification of Streptomyces aburabiensis Producing Cathepsin B Inhibitor (Cathepsin B 저해물질을 생산하는 Streptomyces aburabiensis의 분리 및 동정)

  • 박상진;이현숙;김인섭;김형태;윤성준;이계준
    • YAKHAK HOEJI
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    • v.39 no.3
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    • pp.297-305
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    • 1995
  • The aim of the present study was to develop strains of actinomycetes producing low molecular weight cathepsin B inhibitor. Among 700 isolates from soil samples, a strain of Streptomyces sp. SMF30 producing cathepsin B inhibitor showing specificity and heat stability was selected by an economical and effective screening method. 50 units characteristics for major cluster analysis and 34 units characteristics for minor cluster were tested and the data were analyzed numerically using the TAXON program. The Isolate SMF30 was identified as a strain of Streptomyces aburabiensis

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Regulation of extracellular alkaline protease biosynthesis in a strain of streptomyces sp. (Streptomyces sp. 일주에서 균체외 호염기성 단백질분해 효소의 생합성 조절)

  • 신현승;이계준
    • Korean Journal of Microbiology
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    • v.24 no.1
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    • pp.32-37
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    • 1986
  • In fermentation studies it revealed that Streptomyces sp. SMF 3001 started to synthesize extracellular alkaline protease from early exponential phase of cell growth. The biosynthesis of the alkaline protease was greatly induced by skim milk as a sola nitrogen source and further stimulation was observed under inorganic sulphur limited culture. However, it was found that the biosynthesis was apparently repressed by $NH_4^+$ and free amino acids, specially by cysteine. It was considered that the strain SMF 301 of Streptomyces sp. would produce the alkaline protease for the uptake of sulphur compounds from protein contained in the culture broth.

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Empirical Equations for Checking Validity of Access Hole Parameters for WUF-W Connections (WUF-W 접합부의 액세스 홀 형상변수 타당성 평가를 위한 경험식 제안)

  • Han, Sang Whan;Yoon, Yong;Kim, Y. Debbie
    • Journal of Korean Society of Steel Construction
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    • v.29 no.4
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    • pp.303-310
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    • 2017
  • The welded unreinforced flange-welded web(WUF-W) connection is an all-welded moment connection, which is qualified for special moment frame(SMF) connections. However, previous studies reported that some WUF-W connections did not meet the drift requirement specified for SMF connections and such unexpected connection performance was resulted from weld access hole geometry. The objective of this study is to determine the permissible ranges of major access hole parameters such as access hole slope and the length of flat portion that lead to the satisfactory performance of WUF-W connections using Nonlinear finite element analyses are conducted. This study also proposes simple empirical equations to check the validity of access hole parameters to be selected in design process.

Spot-size converter design of an $1.3\mu{m}$ SSC-FP-LD for optical subscriber network (광가입자용 $1.3\mu{m}$ SSC-FP-LD의 모드변환기 구조 설계)

  • 심종인;진재현;어영선
    • Korean Journal of Optics and Photonics
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    • v.11 no.6
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    • pp.411-417
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    • 2000
  • The waveguide structure effects of a spot-size converter (SSe) of a $1.3\mu{m}$ FP(Fabry-Perot)-LD(Laser Diode) were investigated. Its coupling efficiency and alignment tolerance with a single-mode fiber (SMF) were carefully examined by using a 3dimensional BPM (Beam Propagation Method). It was shown that the fOlmation of enough length of straightened waveguide around the end of the sse region can substantially improve the optical coupling efficiency for a vertically tapered sse. In contrast, a down-taper structure for a laterally tapered sse has superior characteristics to an up-tapered one. We suggested good sse structures which can provide a high coupling efficiency as well as a large alignment tolerance with an .SMF. .SMF.

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Biological Effects of Static Magnetic Fields and ELF-Electromagnetic Field on Microcirculation in Animals

  • Ohkubo, Chiyoji;Okano, Hidyuki;Xu, Shenzhi;Gmitrov, Jraj
    • Proceedings of the Korea Electromagnetic Engineering Society Conference
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    • 1999.07a
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    • pp.117-129
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    • 1999
  • Acute effects of locally applied of static magnetic field (SMF) and extremely low frequency electromagnetic field(ELF-EMF) to the cutaneous tissue within a rabbit ear chamber (REC)were evaluated under conscious conditions. Rabbits with the REC were subjected to intravital microscopical investigation by use of microphotoelectric plethysmography(MPPG). There was no dose-response relationship between the extent of vasomotion changes and frequencies(0,20,50, 100Hz)or power levels (1, 5, 10, 25, 50, 100, 200 mT). Under low vascular tone the both fields induce vasodilatation. The effects of SMF (1 mT) on the cutaneous microcirculatory system induced the vasodilatation with enhanced vasomotion under nor-adrenaline-induced high vascular tone as well as the vasoconstriction with reduced vasomotion under acetylcholine-induced low vascular tone. This suggests that the SMF can modulate vascular tone due to the modification of vasomotion biphasically in the cutaneous tissue.

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Study on the Mechanical Properties of Power Metallurgy Spline Hub for Clutch Disc (클러치 디스크용 분말야금 스플라인 허브의 기계적성질에 관한 연구)

  • 최문일;장진호;강성수
    • Transactions of the Korean Society of Automotive Engineers
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    • v.6 no.5
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    • pp.104-110
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    • 1998
  • In automotive industries, various processes for the cost reduction have been investigated lively. As one of them, powder metallurgy becomes influential. Compared to other methods used for he manufacture of steel components the Powder metallurgy process is competitive primarily due to the small number of production steps to reach the final geometry and thereby also the energy-efficiency. In this paper, to alter present forging process into powder metallurgy process by which the automotive clutch disc spline hub is manufactured machining process, the mechanical properties of sintered materials is investigated by specimen test. Selecting the 3 kinds of materials-SMF 4040, SMF 9060 and DHP-1, their properties according to heat treatment such as carburizing -tempering and plasma-nitrodizing are compared. By result of specimen test - tensile test, compression ring test, Impacting test, measurment of hardness, and microstructure analysis - we concluded that SMF 9060 and carburizing-tempering heat treatment is an optimal material and heat treatment method for the spline hub. It will be able to reduce manufacturing cost and weight.

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Cloning and Expression of the Extracellular $\beta$-lactamase gene from streptomyces sp. SMF13 in streptomyces lividans

  • Rak, Choi-Sang;Lee, Kye-Joon
    • Korean Journal of Microbiology
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    • v.30 no.3
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    • pp.149-153
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    • 1992
  • Cloning of the gene encoding extracellular .betha.-lactamase from Streptomyces sp. SMF13 in a plasmid pIJ702 and expression of the gene in Streptomyces invidans were carried out. Optimal conditions for the formation of protoplasts of S.lividans and the regeneration of the protoplasts were evaluated. Streptomyces sp. SMF-13 was selected as a donor strain of .betha.-lactamase gene and totla DNA of the strain was partially digested with Sau3A I. DNA fragments ranged from 4kb to 10 kb were ligated to pIJ702 AT Bgl II site and then the ligated DNAs were transformed to the protoplasts of S, livivans. The transformation efficiency was $2 *10^{3}$ .$\mu$g DNA for the ligated DNA mixture. One colony among a thousand colonies regenerated showed extracellular .betha.-lactamase and the size of the inserted DNA fragment was estimated to be 3.94 kb. The .betha.-lactamase activity in the culture broth of the recombinant strain was maximum at 3 days culture to be 1.0 unit/ml.

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Dispersion Distributed Fiber Optic Cable for Large-Capacity DWDM System (대용량 DWDM 시스템을 위한 분산 분포 광케이블)

  • Park, Euy-Don;Lee, Dong-Uk;Park, Hae-Young;Kim, Dae-Won;Chung, Yun-C;Son, Hyun;Cho, Yung-Ki
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.26 no.10B
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    • pp.1343-1352
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    • 2001
  • DWDM 전송 시스템에서 전송 용량 증대를 위하여 bit rate를 고속화하고 채널 간격을 줄이기 위해서는 전송로로 사용되는 광케이블의 분산특성이 상반되는 값을 갖도록 요구된다. 즉, 분산으로 인한 신호 왜곡을 줄이기 위해서는 광케이블의 분산이 작아야 하고, 비선형 현상을 억제하기 위해서는 분산이 커야 한다. 이러한 두 가지 상반된 분산 penalty를 동시에 해결하기 위한 방안으로 양과 음의 분산 값이 이산적으로 분포된 광섬유 케이블을 최초로 설계 및 제작하였다. 이러한 분산 분포 케이블을 위해 일반 단일 모드 광섬유(SMF)와 대응되는 음분산 값을 갖는 새로운 광섬유(NDF)를 설계 및 제조한 후, SMF의 +17 ps/km/nm 및 NDF의 -15 ps/km/nm의 분산영역이 6km의 케이블 내에 분포되도록 케이블을 제작하였다. 새롭게 개발된 케이블의 분산 값을 국지적으로는 높은 값을 가지면서도 전체적으로는 1.0 ps/km/nm의 평균 분산 값으로 NZSDF 대비 30% 이하를 가질 수 있음을 확인하였다. 또한, 제안된 케이블은 SMF 케이블과 동일한 케이블 시공 운용 환경에서도 정합함을 확인하였다.

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Effect of a Static Magnetic Field on Susceptibility to Ethanol-Induced Hepatic Dysfunction in Rats

  • Park, Kap-Joo;Kim, Eun-Jung;Cho, Myung-Hwan;Lee, Jae-Seok
    • Food Science and Biotechnology
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    • v.15 no.6
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    • pp.833-837
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    • 2006
  • To determine whether alcohol-treated rat liver cells can be protected by a static magnetic field (SMF), we analyzed the blood chemistry and histology of hepatic tissue removed from alcohol-exposed rats that had been exposed to a static magnetic field. The rats were exposed to a 0.3 tesla (3,000 gauss) magnetic field (MF) for 24 hr daily for 5 weeks with appropriate controls. Glutamic pyruvic transaminase activity and the triglyceride levels in animals exposed to the north (N) or south (S) pole of the MF decreased significantly (p<0.01 and p<0.05, respectively) compared with negative control animals with alcohol exposure. A histological examination of hepatic tissue revealed a moderate to severe accumulation of fat vacuoles of various sizes in the cytoplasm of the hepatocytes of animals in the negative control group throughout the study; whereas in groups exposed to the MF poles, fewer fat vacuoles were seen compared with the negative control group. Electron microscopic observations showed that exposure to the N or S pole protected organelles, including the nucleus, from damage during exposure to this toxic agent, as indicated by the fact that the nucleus and the mithochondria virtually retained their shape throughout this study. These results suggest that exposure to a SMF could be an excellent way of protecting against alcohol-induced damage to the rat liver cell.