Korean Journal of Microbiology (미생물학회지)
- Volume 30 Issue 3
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- Pages.149-153
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- 1992
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- 0440-2413(pISSN)
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- 2383-9902(eISSN)
Cloning and Expression of the Extracellular $\beta$ -lactamase gene from streptomyces sp. SMF13 in streptomyces lividans
- Rak, Choi-Sang (Department of Microbiology and Research Center for Molecular Microbiology Collage of Natural Science,Seoul National University) ;
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Lee, Kye-Joon
(Department of Microbiology and Research Center for Molecular Microbiology Collage of Natural Science,Seoul National University)
- Published : 1992.06.01
Abstract
Cloning of the gene encoding extracellular .betha.-lactamase from Streptomyces sp. SMF13 in a plasmid pIJ702 and expression of the gene in Streptomyces invidans were carried out. Optimal conditions for the formation of protoplasts of S.lividans and the regeneration of the protoplasts were evaluated. Streptomyces sp. SMF-13 was selected as a donor strain of .betha.-lactamase gene and totla DNA of the strain was partially digested with Sau3A I. DNA fragments ranged from 4kb to 10 kb were ligated to pIJ702 AT Bgl II site and then the ligated DNAs were transformed to the protoplasts of S, livivans. The transformation efficiency was
Keywords
- Streptomyces lividans;
- $\beta$-lactamase;
- cloning;
- protoplasts;
- $\beta$-lactam antibioties;
- $\beta$-lactamase gene