• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.549-553
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• 2003

SMB(simulated moving bed) chromatography system has been developed to realize continuous separation and save solvent consumption for binary mixture such as chiral compounds in especial. The parameters of SMB chromatography system can be calculated from mass balance equations of true moving bed chromatography, and they are used in design of 6-column SMB chromatography. We can separate 1'R-25 and 1'S-2S enantiomers as a raffinate product in 95% of purity using assembled SMB chromatography system.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.506-510
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• 2003

SMB(simulated moving bed) chromatography system has been developed to realize continuous separation and save solvent consumption for binary mixture in especial. The parameters of SMB chromatography system can be calculated from mass balance equations of true moving bed chromatography, and they are used in design of 6-column SMB chromatography. We can separate S-ketoprofen enantiomer as a raffinate product in 85% of purity and 0.3mg/ml using assembled SMB chromatography system.

• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.4
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• pp.285-291
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• 2004

A simulated moving bed (SMB) chromatography system is a powerful tool for preparative scale separation, which can be applied to the separation of chiral compound. We have de-signed our own lab-scale SMB chromatography using 5 HPLC pumps, 6 stainless steel columns and 4 multi-position valves, to separate a racemic mixture of ketoprofen in to its enantiomers. Our design has the characteristics of the low cost for assembly for the SMB chromatography and easy repair of the unit, which differs from the designs suggested by other investigators. It is possible for the flow path through each column to be independently changed by computer control, using 4 multi-position rotary valves and 5 HPLC solvent delivery pumps. In order to prove the operability of our SMB system, attempts were made to separate the (S)-ketoprofen enantiomer from a ketoprofen racemic mixture. The operating parameters of the SMB chromatography were calculated for ketoprofen separation from a batch chromatography experiment as well as by the triangle theory. With a feed concentration of 1 mg/mL, (S)-ketoprofen was obtained with a purity of 96% under the calculated operating conditions.

• KSBB Journal
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• v.15 no.6
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• pp.541-547
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• 2000

Simulated moving bed (SMB) chromatography has been investigated in order to separate chiral compounds for pharmaceutical use. SMB utilizes the principle of true moving bed (TMB) chromatography, and has the advantages of low solvent usage, flexible configuration of columns and hardwares, and high productivity of the chiral compounds over the TMB. Large scale separation of xylene isomers and saccharides has been conducted since 1960s. However, the application of SMB in the fine chemical industries is still in the infant stage. The study of SMB for the chiral compounds production was initiated in the mid 1990s and further researches are actively undergoing. This review summarizes the principle of SMB as well as the chemistry of chiral stationary phase and chiral compounds.

• KSBB Journal
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• v.23 no.2
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• pp.135-141
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• 2008

SMB (simulated moving bed) chromatography is a very useful utility for the separation of binary system. We simulated the separation of L-arabinose and L-ribose from the mixture by using lab-scale 4(1-1-1-1)-zone SMB chromatography. Preliminary experiments of PIM (pulse input method) were performed to measure adsorption isotherms of L-ribose and L-arabinose in $NH_2$ HPLC column, and experimental and simulated results from ASPEN chromatography were compared. To find the most suitable separation condition in SMB, we carried out a simulation in $m_2-m_3$ plane base on the triangle theory and calculated operating parameters (flow rate of four zone, switching time and feed concentration and so on) using ASPEN chromatography under the conditions of linear isotherms obtained from PIM.

• Korean Chemical Engineering Research
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• v.49 no.6
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• pp.798-803
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• 2011

IgY(Immunoglobulin Yolk) is a specific antibody in egg yolk, and it protects human body from virus and antigen. There are a lot of egg yolk components such as lipoprotein and protein. To separate IgY, HPLC(High Performance Liquid Chromatography) and precipitation were used in a batch mode and SMB(Simulated Moving Bed) was adopted for continuous purification of yolk proteins. IgY and other proteins in yolk were separated by using three-zone SMB chromatography. Before performing SMB experiments, batch chromatography and PIM(pulse input method) were performed to find operation parameters and adsorption isotherms. The results of batch chromatography were compared with simulated results using Aspen chromatography. To find the most suitable separation condition in SMB chromatography, simulations in $m_2$-$m_3$ plane on the triangle theory were carried out. $m_2$ = 0.18, $m_3$ = 1.0 and ${\Delta}$t = 419 s are the best conditions for the highest purity of IgY. With this operating parameters(flow rate in three zone and switching time), the purity of raffinate results in 98.39% from Aspen chromatography simulation. Most of the simulation reached steadystate within second recycle.

• Korean Chemical Engineering Research
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• v.50 no.5
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• pp.866-873
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• 2012

IgY (Immunoglobulin Yolk) is a specific antibody in egg yolk, and it protects human body from virus and antigen. There are a lot of egg yolk components such as lipoprotein and protein. To separate IgY, HPLC (High Performance Liquid Chromatography) and precipitation were used in a batch mode and SMB (Simulated Moving Bed) was adopted for continuous purification of yolk proteins. IgY and other proteins in yolk were separated by using three-zone and four-zone SMB chromatography. Before performing SMB experiments, batch chromatography simulation parameters and adsorption isotherms were obtained. The parameters of batch chromatography were used to simulate SMB using Aspen chromatography. To compare three-zone and four-zone SMB chromatography, simulations in $m_2-m_3$ plane on the triangle theory were carried out. In terms of concentration and purity of both IgY and other lipoproteins, 3-zone SMB process is considered as ideal at the vertex of triangle ($m_2$, $m_3$=0.1, 1.1). 4-zone SMB yields the highest IgY purity at the coordinate ($m_2$, $m_3$=0.06, 0.5), which is the pure raffinate region. In 3-zone SMB without recycle, other lipoproteins in extract are largely affected in purity by small shift from the vertex of triangle ($m_2$, $m_3$=0.1, 1.1).

• KSBB Journal
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• v.20 no.3
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• pp.177-182
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• 2005

Simulated moving bed (SMB) chromatography was used in the petrochemical industry in the 1960s and its use has been widened to separate chiral compounds in the 1990s. Chiral stationary phase (CSP) is the key component in SMB for the separation of the chiral compounds. CSP has been developed for the analytical use in HPLC, however, its development successfully adapted for the preparative use in SMB chromatography. This review covers the present state-art technology of CSP for SMB chromatography in terms of selectivity, stability, and capacity.

• Biotechnology and Bioprocess Engineering:BBE
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• v.6 no.6
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• pp.363-375
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• 2001

Chromatography has been method of choice for the separation complex biologi-cal mixtures fro analytical purpose, particularly for the last fifty years. Its use has recently been extended to preparative separation where the productivity relative to the amount of resin and sol-vent used is a matter of concern. To overcome the inherent thermodynamic inefficiency of batch chromatography, as exemplified by the partial temporal usage of the resin and dilution of the product with the solvent, chromatography has been continually modified by separation engineers. Column switching and recycling represnet some of the process modifications that have brought high productivity to chromatography. Recently, the simulated moving bed (SMB) method, which claims a high separation efficiency based on counter-current moving bed chromatography. has be-come the mainstay of preparative separation, especially in chiral separation. Accordingly, this pa-per reviews the current status of SMB along with several chromatographic modification, which may be helpful in routine laboratory and industrial chromatographic practices.

• Korean Chemical Engineering Research
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• v.49 no.5
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• pp.623-627
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• 2011

Simulated moving bed(SMB) chromatography was simulated for separating loxoprofen racemates. Aspen chromatography simulator was utilized with Henry's constants of loxoprofen racemates which were obtained by batch chromatography experiments. Raffinate stream concentrations as well as purities were calculated with various $m_2$ and $m_3$ values in the triangle diagram obtained from two Henry's constants 7.9 and 10.1. Purity values are high under the conditions that the $m_2-m^3$ coordinates are near the left central region in the diagram and feed flow rates are lower. Concentration profiles of raffinate and extract streams along SMB columns explain the purity change in the case of increasing the column numbers installed at SMB.