• Journal of Acupuncture Research
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• 제20권5호
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• pp.50-62
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• 2003

Objective: Bee Venom(BV) has been used for various kinds of inflammatory or painful conditions in Oriental Medicine clinics, and there publishes reports on its therapeutic effects and the probable mechanism of those therapeutic effects, where CDs and cytokines plays important role. This study investigated the influences of bee venom on the expressions of CDs and cytokines of HMC cell line Methods: In this study we analysed the expression profile of HMC cell line treated with BV of 10-2ug/ml in relation to that of HMC cell line treated with vehicle by way of CD/cytokine microarray hybridization with 342 genes on it. Results: There were no upregulated genes by more than 3 fold, while there showed some downregulated genes by less than 1/3 fold as follows: colony stimulating factor 2, CD122, IL-7, CD112, TNF-alpha, CD138, CD166, TGFbetaR2, CD42b, CD62L, CD111, interleukin 10 receptor alpha, colony stimulating factor 1(macrophage), CD38 antigen(p45), CD121a, CD33 antigen(gp67), colony stimulating factor 1 receptor, B cell linker protein (SLP65) mRNA, CD94, alanyl(membrane) aminopeptidase, immunoglobulin(CD79A) binding protein 1, CD205, CD241, CD207, CDw121b, integrin alpha L(CD11a), integrin beta 1(CD29), CD91, CD42b. Conclusions: Bee venom treatment induced downregulation of some CDs or cytokines including $TNF-{\alpha}$. IL-1R with its possible implication in an antiinflammatory action of BV. Further research on expression profile changes induced by BV treatment is expected.

• 대한후두음성언어의학회지
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• 제17권1호
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• pp.43-48
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• 2006

Background and Objectives: Countertenors who can produce higher vocal pitch like female classical singer's voice and use both modal and falsetto register. This study was conducted to study phonatory characteristics between modal and falsetto register of the countertenor. Materials and Methods: A male countertenor who had 8 years of experience was examined using a videostroboscopy and his voice was analyzed using aerodynamic measures; fundamental frequency(F0), Mean air flow rate(MFR), intensity(SLP), subglottal air pressure(Psub) with phonatory function analyzer(Nagashima) and acoustic measures; jitter, shimmer, HNR, closed quotient(CQ) using a Electro-glottography(EGG) of Lx. Speech Studio(Laryngoscope, Ltd, UK) and voice range profile of CSL(Kay elemetrics). Results: In the stroboscopy finding, the longitudinal length of vocal folds was increased at the falsetto register and the upper margin of vocal folds vibrated with incomplete closure of true vocal folds. In aerodynamic analysis, intensity was same at the modal and falsetto register. However, MFR, Psub, MPT were higher at the falsetto register. In the electroglottographic analysis, closed quotient(CQ) at the modal register was high and also much higher at the high-pitch falsetto than at the loud falsetto. In the VRP, intensity was similar though F0 was different between modal and falsetto register. Conclusion: It implied that countertenor could produce powerful voice quality by increasing of respiratory pressure and respiratory volume though glottal closure was incomplete. In addition, no change of EGG waveform, similar voice range with alto was observed.

• 한국항행학회논문지
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• 제18권5호
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• pp.508-512
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• 2014

본 논문에서는 RF시스템을 이용하여 도로 가로등의 자기제어와 수동제어에 대한 알고리즘과 제어시스템을 제안하였다. 자기제어 알고리즘에는 그룹제어와 개별제어가 있다. 그룹제어의 경우, 도로 가로등에 설치된 시스템의 물체감지센서를 통해 차량이 감지되면 RF신호를 송신하여 그룹별로 도로 가로등이 점등되며, 개별제어의 경우 도로의 갓길에 주차된 차량이나 사람을 도로 가로등이 인식하면 개별적으로 점등된다. 또한, 관리자가 RF신호와 각종센서를 통해 도로 가로등의 상태와 주변 환경도 점검 할 수 있는 수동제어 알고리즘도 있다. 따라서, 제안된 시스템을 통해 무의미하게 소모되는 에너지를 절감할 수 있으며, 도로 가로등의 유지 관리에 있어서도 편의성을 제공한다.

• 한국자동차공학회논문집
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• 제12권3호
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• pp.101-108
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• 2004

A FEM-based efficient method is developed for the shape optimization of 2-D structures. The combined SLP and Simplex method are coupled with finite element analysis. Selected set of master nodes on the design boundaries are employed as design variables and assigned to move towards their normal directions. The other nodes along the design boundaries are grouped into the master node. By interpolating the repositioned master nodes, the B-spline curves are formed so that the rest mid-nodes efficiently settle down on the B-spline curves. Mesh smoothing scheme is also applied for the nodes on the design boundary to maintain most finite elements in good quality. Finally, a numerical implementation of optimum design of an automobile torque converter piston subjected to pressure and centrifugal loads is presented. The results shows additional weight up to 13% may be saved after the shape optimization.

• 한국항공우주학회지
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• 제31권8호
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• pp.27-35
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• 2003

쉘의 기하 모델링과 해석은 각각 다른 배경과 목적을 가지고 발전되어 왔다. 따라서 기하 모델링과 해석을 통합한 설계 도구를 만들기에 기존의 방법은 적절하지 않다. 본 연구에서는 기하 모델링과 해석, 최적 설계를 통합한 개념을 제시한다. 이것은 B-스플라인 곡면의 표현방법에 기초를 두고 있다. 기하학적으로 정확한 쉘 유한요소를 도입하였으며, 최적 설계 부분에서는 곡면의 조정점을 설계변수로 택하였다. 또한 설계 민감도를 계산하기 위해서 준해석적 방법을 사용했고, 이를 바탕으로 순차적 선형계획법을 이용해 곡면의 형상 최적화를 수행하였다. 이렇게 개발된 통합설계 개념은 곡면의 모델링과 해석에 적합한 도구로 이용될 수 있다.

• 한국미생물·생명공학회지
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• 제29권1호
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• pp.1-11
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• 2001

Lactic acid bacteria (LAB) are widely used for various food fermentation. With the recent advances in modern biotechnology, a variety of bio-products with the high economic values have been produced using microorganisms. For molecular cloning and expression studies on the gene of interest, E. coli has been widely used mainly because vector systems are fully developed. Most plasmid vectors currently used for E, coli carry antibiotic-resistant markers. As it is generally believed that the antibiotic resistance markers are potentially transferred to other bacteria, application of the plasmid vectors carrying antibiotic resistance genes as selection markers should be avoided, especially for human consump-tion. By contrast, as LAB have some desirable traits such that the they are GRAS(generally recognized as safe), able to secrete gene products out of cell, and their low protease activities, they are regarded as an ideal organism for the genetic manipulation, including cloning and expression of homologous and heterologous genes. However, the vec-tor systems established for LAB are stil insufficient to over-produce gene products, stably, limiting the use of these organisms for industrial applications. For a past decade, the two popular plasmid vectors, pAM$\beta$1 of Streptococcus faecalis and pGK12 theB. subtilis-E. coli shuttle vector derived from pWV01 of Lactococcus lactis ssp. cremoris wg 2, were most widely used to construct efficient chimeric vectors to be stably maintained in many industrial strains of LAB. Currently, non-antibiotic markers such as nisin resistance($Nis^{r}$ ) are explored for selecting recombi-nant clone. In addition, a gene encoding S-layer protein, slp/A, on bacterial cell wall was successfully recombined with the proper LAB vectors LAB vectors for excretion of the heterologous gene product from LAB Many food-grade host vec-tor systems were successfully developed, which allowed stable integration of multiple plasmid copies in the vec-mosome of LAB. More recently, an integration vector system based on the site-specific integration apparatus of temperate lactococcal bacteriophage, containing the integrase gene(int) and phage attachment site(attP), was pub-lished. In conclusion, when various vector system, which are maintain stably and expressed strongly in LAB, are developed, lost of such food products as enzymes, pharmaceuticals, bioactive food ingredients for human consump-tion would be produced at a full scale in LAB.

• 한국공간구조학회:학술대회논문집
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• 한국공간구조학회 2008년도 춘계 학술발표회 논문집
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• pp.101-106
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• 2008

본 연구에서는 서로 상이한 최적화기법인 유전알고리듬과 수학적 프로그래밍기법을 이용하여 지오데식 돔 구조물을 최적화하고 그 결과를 분석하였다. 본 연구에서 도입한 유전알고리즘은 유전연산자인 선택, 교배, 돌연변이 이외에도 재생기법을 도입하여 최적해의 검색성능을 높였다. 그리고 수학적인 프로그래밍기법은 유한차분법을 이용하여 목적함수의 설계민감도를 계산하였으며 세 가지의 다른 검색기법을 이용하여 돔의 크기최적화를 수행하였다. 지오데식 돔의 중앙에 작용하는 집중하중에 저항하는 돔의 각 부재의 크기패턴을 자체 개발된 $ISADO-GA{\alpha}$와 ISADO-OPT를 이용하여 최적 설계하였다. 본 연구를 통하여 제시된 최적결과는 정해가 존재하지 않는 실제 구조물의 최적 값에 대한 유용한 정보를 제공할 뿐만이 아니라 향후 대공간구조의 새로운 구조시스템 개발의 밑거름이 될 것으로 판단된다.

• 대한토목학회논문집
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• 제5권1호
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• pp.1-12
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• 1985

본(本) 연구(硏究)는 직접설계법(直接設計法)에 의한 다층(多層), 다경간(多徑間)을 갖는 철근(鐵筋)콘크리트 슬래브형(型) 구조체(構造體)의 최적화(最適化)에 관한 것이다. 설계제약조건(設計制約條件)으로는 슬래브, 보, 기둥의 휨강도(强度), 전단강도(剪斷强度), 단면치수, 철근비(鐵筋比) 등을 고려했고 목적함수(目的函數)로는 공비함수(工費函數)를 취했는데 이들은 고차(高次)의 비선형계획문제(非線型計劃問題)가 된다. 본(本) 연구(硏究)에서는 축차선형계획기법(逐次線型計劃技法)을 사용하여 전체(全體) 구조(構造)를 종합적(綜合的)으로 최적화(最適化) 할 수 있는 최적설계(最適設計) 프로그램을 개발하였다. 개발된 알고리즘의 타당성(妥當性), 경제성(經濟性), 수렴성(收斂性) 등을 확인하기 위해 5층(層)의 구조체(構造體)로서 각층(各層), 각 경우에 대해 직접 적용(適用)하였다. 그 결과 3~5회 반복시행(反復試行)으로 최적해(最適解)에 수렴(收斂)했고 재래(在來)의 설계(設計)에 비(比)해 경제적(經濟的)임이 입증(立證)되었다.

• International journal of advanced smart convergence
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• 제9권2호
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• pp.8-19
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• 2020

We see an energy fucused smart city evolution of the UK along with the project of "Smart London Plan (SLP)." A theoretical logic of business model innovation has been discussed and a research framework of evolving energy focused smart city is formulated. The starting point is the silo system. In the second stage, the private investment in smart meters establishes a basement for next stages. As results, the UK's smart energy sector has evolved from smart meter installation through smart grid to new business models such as water-energy nexus and microgrid. Before smart meter installation of the government, the electricity system was centralized. However, after consumer engagement plan has been set to make them understand benefits that they can secure through smart meters, the customer behavior has been changed. The data analytics firm enables greater understanding of consumer behavior and it helps energy industry to be smart via controlling, securing and using that data to improve the energy system. In the third stage, distribution network operators (DNOs)' access to smart meter data has been allowed and the segmentation starts. In the fourth stage, with collaboration of Ofwat and Ofgem, it is possible to eliminate unnecessary duplication of works and reduce interest conflict between water and electricity. In the fifth stage, smart meter and grid has been integrated as an "adaptive" system and a transition from DNO to DSO is accomplished for the integrated operation. Microgrid is a prototype for an "adaptive" smart grid. Previous steps enable London to accomplish a platform leadership to support the increasing electrification of the heating and transport sector and smart home.

• 대한토목학회논문집
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• 제9권3호
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• pp.57-64
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• 1989

철근콘크리트 뼈대구조의 신뢰성 최적설계에 관한 연구를 제시한 것으로 파괴확률의 산정에서 AFOSM방법과 SOSM방법을 적용하였고, SLP 알고리즘을 바탕으로 하는 시스템 최적화 기법을 사용하였다. 신뢰성 최적설계를 예시할 목적으로 1층 2경간 철근콘크리트 뼈대에 대한 수치예를 제시하였다. 결과적으로 여기서 적용된 신뢰성 최적화 과정은 최적수준의 안전률은 비교적 정확하게 예측할 수 있게하며 어느정도 복잡한 실제 구조물에도 사용될 수 있다는 사실을 알수 있었다. 아울러 기본적인 변수의 확률분포 및 작용하중과 재료의 불확실성에 관한 민감도는 최적설계에 큰 영향을 미칠 수 있으며 AFOSM방법과 SOSM방법은 설계결과의 영향에 별 차이가 없으므로 본 연구 경우로 보아 시간적으로 경제성이 있는 AFOSM방법이 더 실용적이라고 볼 수 있다.