• Proceedings of the Korea Society for Simulation Conference
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• 2002.05a
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• pp.21-32
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• 2002

In accordance with the development plans of Ulsan harbor, Ulsan new harbor will be contructed considering supporting Ulsan harbor as a safe berthing and departure at single buoy mooring(SBM). In this study, we used a full-mission ship handling simulator adopting 300,000 DWT VLCC manoeuvered at the planned Ulsan SBM. Five masters who have had a long experience of ship maneuvering were called to carry out the simulations, of which each scenario were tried one, completed total of 68 times. The marine traffic safety was assessed in terms of 1) the closest point of approach(CPA) to other SBM and breakwater in the vicinity and the probability of crossing the restricted area of the closest SBM and fairway limit, 2) subjective evaluation such as the mental burden and the maneuvering difficulty of shiphanders, and 3) the opinions of shiphandlers. From the result of this simulation, we have a conclusion as follows; First, because crude oil berthing angle is so small by current S-OiL Co. crude oil buoy by SK Co. No 3 crude oil buoy different view SK Co. No 3 crude oil buoy and interference of current KNOC crude oil buoy, Berthing is impossible, and Emergency departing is very dangerous too operation impossible. Second it is desirable that SK Co. No 2 and No 3 Single buoy Mooring that do different view controls position so that to be not put in straight line each other. Third, SK Co. No 1 and 2 single buoy mooring that do different view to Onsanhang berthing and departing is seized by single buoy mooring by external force ship that set sail does faith control need.

• Journal of the Korean Magnetics Society
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• v.13 no.2
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• pp.59-63
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• 2003

Superparamagnetic properties of self-aggregated cobalt nanoparticles in the perfluorinated ion-exchange polymeric membrane (MF-4SK) prepared by ion-exchange and recovery methods were investigated by transmission electron microscopy (TEM) and superconducting quantum interference device (SQUID) magnetometer at various temperatures. Our experimental results show that cobalt nanoparticles in MF-4SK for the concentration of $7.8{\times}10^{19}$ atoms per 1 g of polymer membrane exhibit superparamagnetic properties above the average blocking temperature ($T_{B}$), which is determined to be around 185 K at applied field of 500 Oe. The average particle radius of 4.0 nm achieved from Langevin function fit is in good agreement with TEM observations. This experimental evidence suggests that cobalt nanoparticles in polymer film obey a single domain theory. The results are discussed in the light of current theory for the superparamagnetic behavior of magnetic nanoparticles.

• Biomolecules & Therapeutics
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• v.15 no.4
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• pp.205-211
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• 2007

The fruits and stems of Opuntia ficus-indica var. saboten have been reported to exhibit a variety of pharmacological actions, including antioxidant, analgesic, anti-inflammatory, and anti-ulcer effects. In the present study, we evaluated effects of the butanol fraction (SK OFB901) prepared from the 50% ethanol extract of the stems on various types of neuronal injuries induced by oxidative stress, excitotoxins, and amyloid ${\beta}\;(A_{\beta})$ in primary cultured rat cortical cells. Its antioxidant and radical scavenging activities were also evaluated by cell-free bioassays. We found that SK OFB901 strongly inhibited the oxidative neuronal damage induced by $H_2O_2$ or xanthine/xanthine oxidase. In addition, it exhibited marked inhibition of the excitotoxic neuronal damage induced by glutamate, N-methyl-D-aspartic acid, or kainate. Furthermore, the $A_{\beta(25-35)}$-induced neurotoxicity was also significantly attenuated by SK OFB901. It was found to inhibit lipid peroxidation initiated by $Fe^{2+}$ and L-ascorbic acid in rat brain homogenates and scavenge 1,1-diphenyl-2-picrylhydrazyl free radicals. These results indicate that the butanol fraction prepared from the stems of Opuntia ficus-indica var. saboten exerts potent antioxidant and neuroprotective effects through multiple mechanisms, implying its potential applications for the prevention or management of neurodegenerative disorders associated with oxidative stress, excitotoxicity, and $A{\beta}$.

• Journal of Plant Biotechnology
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• v.30 no.4
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• pp.335-339
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• 2003

Several experiments were carried out to transfer LEAFY gene to Dendranthema grandiflora 'Shuho-no-chikara' by Agrobacterium LBA4404 carrying pSK109 encoding LEAFY gene. Kanamycin 10mg/L was used in first selection medium, and 20mg/L in the second one. Co-culture for 3 days was more effective in increasing transformation efficiency than that for 7 days. The transformation efficiency by Agrobacterium LBA4404 carrying pSK109 encoding LEAFY gene was about 2.8％ until the second selection, but only 0.13％ of shoots (two plants) was confirmed as a transgenic plants in Southern analysis. The escape of putative transformants was occured seriously in the process of selections, PCR analysis for confirming of neomycin phosphotransferaseII (npt II), and Southern analysis for LEAFY gene. One transgenic plant appeared 7 days'early flowering in field.

• Information and Communications Magazine
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• v.17 no.1
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• pp.58-63
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• 2000

새 천년을 맞이하여 국내 이동전화 시장은 규모와 기술적 측면에서 놀랄만한 변화 를 겪게 될 것이다. 음성중심의 2세대 이동전화 서비스는 보급율이 50%를 넘어서며 포화수 준에 근접하고 있으며, 앞으로 무선데이타 통화량이 크게 증가하여 3~4년 후에는 총 통화량 의 50% 이상을 점유할 것으로 예측된다. 통신사업자들은 통신망의 광대역화와 글로벌화, IP Network 화를 더욱 강력하게 추진하고 있으며, 올 연말로 예정된 IMT-2000 사업자 선정은 이러한 추세를 더욱 가속화시킬 것으로 전망된다. 특히, 인터넷과 이동전화의 융화는 인간의 삶과 문화를 한순간에 변모시킬 혁명적 위력을 내포하고 있다. 정보화 시대를 향한 정보통 신업계의 대변혁이 그 절정을 향해 가속도를 붙이기 시작한 것이다. 신세기통신은 이러한 변화와 혼돈의 거친 물결 속에서 21세기 '정보통신 업계의 강자'로 도약하기 위한 힘찬 항해 를 진행하고 있다. 2000년 상반기까지 IS-2000시스템을 구축하여 최대 144kbps 수준의 고속 데이타 서비스를 제공하는 한편 i-TOUCH 017 컨텐츠 (Contents)를 한층 다양화, 첨단화 하는 등 본격적인 이동인터넷 사업을 전개해 나갈 것이다. 또 CDMA 국제 자동로밍 서비스 를 일본, 미국, 유럽 등 전세계로 확대하여 017 서비스의 글로벌화를 이룩할 계획이다. 이러 한 통신망 진화와 이동 인터넷 사업의 상당 부분을 SK텔레콤과 함께 추진하게 될 것이며 이러한 양사간의 전략적 제휴에 따른 시너지 효과는 오는 연말 IMT-2000 사업권의 성공적 확보로 이어질 것으로 기대된다. 21세기 정보통신산업은 광대역 통신망으로 대변되는 하드 웨어와 인터넷 컨텐츠로 대변되는 소프트웨어가 서로 융화되는 방향으로 발전할 것이다. 이에 따라 고객의 서비스 만족도나 사업자 선택 기준은 기존 통화물질 중심에서, 사업자가 제공하는 컨텐츠의 다양성이나 편리성으로 변화하는 것이 국적을 바꾸는 것보다 어려운 시 대가 멀지 않은 미래에 도래할 것이다. 신세기 통신 과 SK 텔레콤에는 현재 1,300만명이 넘 는 고객이 있으며. 이들 고객은 어 이상 음성통화 중심의 이동전화 고객이 아니라 신세기 통신과 SK텔레콤이 함께 구축해 나갈 거대란 무선 네트워크 사회에서 정보화 시대를 살아 갈 회원들이다. '컨텐츠의 시대'가 개막되는 것이며, 신세기통신과 SK텔레콤은 선의의 경쟁 과 협력을 통해 이동인터넷 서비스의 컨텐츠를 개발해 나가게 될 것이다.

• KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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• v.4 no.1
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• pp.3-10
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• 1997

The purpose of this research was to complete basic study about standardization of corrugated fibreboard containers for apples. Various corrugated fibreboard containers are studied and the results are shown below. 1. WLK, SK, and KA are usually used for outer liner boards, S and $K_2$ are for medium or inner liner boards. 2. According to the study, about twenty different liner compositions are currently used for apple packaging. However, for the containers with white liner board, the liner composition is WLK210/M200/S120/$M200/K_{2}200$, for the containers with colored liner board, SK180/M200/S120/$M200/K_{2}200$ are mostly used. 3. Sixteen percents of cost saving are expected when subsitute white outer liner boards by those of colored.

• Archives of Pharmacal Research
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• v.29 no.2
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• pp.131-134
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• 2006

A new compound 2 and two known guaiane-type sesquiterpenoids were isolated from the methylene chloride-soluble fraction of the methanolic extract of the fruits of Torilis japonica (Umbelliferae) through repeated silica gel and Sephadex LH-20 column chromatography. Their chemical structures were elucidated as torilin (1), 11-acetoxy-8-angeloyloxy-$1{\beta}$-hydroxy-4-guaien-3-one ($1{\beta}$-hydroxytorilin, 2), and 11-acetoxy-8-angeloyloxy-$1{\alpha}$-hydroxy-4-guaien-3-one ($1{\alpha}$-hydroxytorilin, 3) by spectroscopic analysis. Compounds 1-3 exhibited cytotoxicity against human A549, SK-OV-3, SK-MEL-2, and HCT15 tumor cells.

• Archives of Pharmacal Research
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• v.24 no.4
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• pp.312-315
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• 2001

Repeated column chromatographic separation of the $CH_{2}Cl_{2}$ extract of Artemisia stolonofera (Asteraceae) led to the isolation of a triterpene (I), a sesquiterpene (II), two aromatic compounds (III and IV) and a benzoquinone (V). Their structures were determined by spectroscopic means to be simiarenol (I), (1S,7S)-1 $\beta$-hydroxygermacra-4(15),5, 10(14)-triene (II), 3'-methoxy-4'-hydroxy-trans-cinnamaldehyde (III), vanillin(IV) and 2,6-dimethoxy-1,4-benzoquinone (V), respectively. Among these products, compound V showed significant cytotoxicity against five human tumor cell lines in vitro, A549 (non small cell lung adenocarcinoma), SK-OV-3 (ovarian), SK-MEL-2 (skin melanoma), XF498 (CNS) and HCT15 (colon) with ED_{50}$ values ranging from 1.33~4.22${\mu}g/ml$.

• The Korean Journal of Physiology and Pharmacology
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• v.12 no.4
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• pp.143-148
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• 2008

Caveolin-1 (CAV1) is an integral membrane protein that may function as a scaffold for plasma membrane proteins and acts as a tumor suppressor protein. One causative factor of chemotherapy-resistant cancers is P-plycoprotein (P-gp), the product of the multidrug resistance-1 gene (MDR1), which is localized in the caveolar structure. Currently, the interactive roles of CAV1 and MDR1 expression in the death of cancer cells remain controversial. In this study, we investigated the effects of indomethacin on the cell viability and the expression levels of MDR1 mRNA and protein in a CAV1-siRNA-mediated gene knockdown hepatoma cell line (SK-Hep1). Cell viability was significantly decreased in CAV1-siRNA-transfected cells compared with that of control-siRNA-transfected cells. Furthermore, the viability of cells pretreated with CAV1 siRNA was markedly decreased by treatment with indomethacin (400${\mu}$M for 24 h). However, the protein and mRNA levels of MDR1 were unchanged in CAV1-siRNA-transfected cells. These results suggest that CAV1 plays an important role as a major survival enzyme in cancer cells, and indomethacin can sensitively induce cell death under conditions of reduced CAV1 expression, independent of MDR1 expression.

• Journal of Life Science
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• v.24 no.3
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• pp.242-251
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• 2014

The objective of this study was to isolate a photosensitizer from Pueraria thunbergiana leaves that induces apoptosis in SK-HEP-1 cells. Column chromatography and thin layer chromatography were used to isolate active compounds from extracts of P. thunbergiana leaves. The structures of the isolated compounds were determined by 1D-NMR, 2D-NMR, and FAB-mass spectroscopy. A substance, named M4-3, was purified from the leaves of P. thunbergiana using various chromatography methods, and the absorbance of the substance was measured. The absorbance was highest at 410 nm, suggesting that the M4-3 substance was a different compound from chlorophyll a and b, which absorb at 410, 502, 533, and 607 nm. Further analyses revealed that the M4-3 compound was a $13^2$-hydoxy pheophorbide, a methyl ester with a molecular weight of 662. M4-3 was identified as a derivative compound of pheophorbide, with a structure that magnesium comes away from the porphyrin ring. The results of the analysis of the cytotoxicity of the M4-3 substance against the SK-HEP-1 cells revealed that it inhibited rates of cell growth by 40% and 80% at a concentration of 0.04 ${\mu}M$ and 0.08 ${\mu}M$, respectively. The M4-3 compound was found to be a photosensitizer for cytotoxicity because it was appeared only in light condition as examining activity in different irradiation conditions (light condition and nonlight condition) under the same concentration. Analysis of morphological changes in the cells following cell death induced by exposure to the M4-3 substance reveled representative phenomena of apoptosis (nuclear condensation, vesicle formation, and fragmentation of DNA). The induction of apoptosis was attributed to the compound's photodynamic activity.