• BMB Reports
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• v.47 no.10
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• pp.581-586
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• 2014

Epidermal growth factor (EGF) is known to play key roles in skin regeneration and wound-healing. Here, we demonstrate that Pep2-YAC, a tripeptide covering residues 29-31 in the B loop of EGF, promotes the proliferation of HaCaT keratinocytes with activity comparable to EGF. The treatment of HaCaT cells with Pep2-YAC induced phosphorylation, internalization, and degradation of EGFR and organization of signaling complexes, which consist of Grb2, Gab1, SHP2, and PI3K. In addition, it stimulated the phosphorylation of ERK1/2 at Thr 202/Tyr 204 and of Akt1 at Ser 473 and the nuclear translocation of EGFR, STAT3, c-Jun, and c-Fos. These results suggest that Pep2-YAC may be useful as a therapeutic agent for skin regeneration and wound-healing as an EGFR agonist.

• Journal of Life Science
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• v.21 no.1
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• pp.141-145
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• 2011

Actinomycin D is a natural antibiotic that is used in anti-cancer chemotherapy and is known as a transcription inhibitor. Interestingly, actinomycin D induces phosphorylation of signal transducers and activators of transcription 3 (STAT3) in renal cancer Caki cells. In this study, we examined the molecular mechanism of actinomycin D-induced STAT3 phosphorylation. Treatment with actinomycin D induced phosphorylation of STAT3 (Tyr705) in a dose- and time-dependent manner. However, actinomycin D did not induce phosphorylation of STAT3 (Ser727), STAT1 (Tyr701) and STAT1 (Ser727). Moreover, actinomycin D-induced STAT3 phosphorylation was caused by decreased protein and mRNA levels of SOCS3, but not by JAK2 and SHP-1. In addition, other transcription inhibitor (5,6-dichloro-1-b-D-ribofuranosyl benzimidazole; DRB) also induced phosphorylation of STAT3 (Tyr705). Taken together, the present study demonstrates that transcriptional inhibitors (actinomycin D and DRB) induce phosphorylation of STAT3 (Tyr705) in Caki cells by down-regulation of SOCS3.

• Membrane Journal
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• v.32 no.6
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• pp.427-441
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• 2022

Polymer electrolyte membrane (PEM) serving as a separator that can prevent the permeation of unreacted fuels as well as an electrolyte that selectively transports protons from the anode to the cathode has been considered a key component of polymer electrolyte membrane fuel cell (PEMFC). The perfluorinated sulfonic acid-based PEMs, represented by Nafion®, have been commercialized in PEMFC systems due to their high proton conductivity and chemical stability. Nevertheless, these PEMs have several inherent drawbacks including high manufacturing costs by the complex synthetic processes and environmental problems caused by producing the toxic gases. Although numerous studies are underway to address these drawbacks including the development of sulfonated hydrocarbon polymer-based PEMs (SHP-PEMs), which can easily control the polymer structures, further improvement of PEM performances and durability is necessary for practical PEMFC applications. Therefore, this study focused on the various strategies for the development of SHP-PEMs with outstanding performance and durability by 1) introducing cross-linked structures, 2) incorporating organic/inorganic composites, and 3) fabricating reinforced-composite membranes using porous substrates.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.52 no.2
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• pp.127-133
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• 2019

Seahorses have long been used as ornamental and medicinal products. The sea horse Hippocampus abdominalis has a beautiful color and unique shape and is also used for ornamental purposes and as a traditional medicine in China. This study examined the value of H. abdominalis as a health functional food or food additive. H. abdominalis was hydrolyzed using seven proteases: flavourzyme, neutrase, alcalase, trypsin, kojizyme, pepsin and protamex. The yields of all of the enzyme hydrolysates were higher than that of the aqueous extract. Of the enzymatic hydrolysates, seahorse Protamex hydrolysate (SHP) gave the highest yield and had excellent antioxidant and angiotensin-I converting enzyme inhibitory activities. It protected Vero cells against oxidative by 2,2-azobis-(2-amidinopropane) dihydrochloride (AAPH) and antihypertension in Spontaneously Hypertensive Rats. This study attempted to demonstrate H. abdominalis as a health functional food or food additive in the future.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.11 no.8
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• pp.1392-1398
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• 2000

In this paper, Sub Harmonic Ring Mixer using Anti-Parallel Diode Pair is studied. Conventional mixers mix LO signal with RF signal and, obtain IF signal from the difference between LO and RF. Sub harmonic ring mixers using APDP mix RF signal with the second harmonic of LO signal, LO frequency needed for conventional receiver is reduced by 1/2.The produced mixer showed 12 dB conversion loss, and 1 dB compression point of IF signal, in respect to RF signal, was found at the 0 dBm RF signal. Isolation LO/IF and LO/RF is 24.6 dB and 22.5 dB respectively. Isolation RF/LO and LO/RF is 32.6 dB and 22.5 dB respectively.

• Korean Journal of Agricultural and Forest Meteorology
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• v.17 no.3
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• pp.261-269
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• 2015

The purpose of this research is to identify the significance of climate factors related to the significance of change of dry matter yield (DMY) of whole crop maize (WCM) by year through the exploratory data analysis. The data (124 varieties; n=993 in 7 provinces) was prepared after deletion and modification of the insufficient and repetitive data from the results (124 varieties; n=1027 in 7 provinces) of import adaptation experiment done by National Agricultural Cooperation Federation. WCM was classified into early-maturity (25 varieties, n=200), mid-maturity (40 varieties, n=409), late-maturity (27 varieties, n=234) and others (32 varieties, n=150) based on relative maturity and days to silking. For determining climate factors, 6 weather variables were generated using weather data. For detecting DMY and climate factors, SPSS21.0 was used for operating descriptive statistics and Shapiro-Wilk test. Mean DMY by year was classified into upper and lower groups, and a statistically significant difference in DMY was found between two groups (p<0.05). To find the reasons of significant difference between two groups, after statistics analysis of the climate variables, it was found that Seeding-Harvesting Accumulated Growing Degree Days (SHAGDD), Seeding-Harvesting Precipitation (SHP) and Seeding-Harvesting Hour of sunshine (SHH) were significantly different between two groups (p<0.05), whereas Seeding-Harvesting number of Days with Precipitation (SHDP) had no significant effects on DMY (p>0.05). These results indicate that the SHAGDD, SHP and SHH are related to DMY of WCM, but the comparison of R2 among three variables (SHAGDD, SHP and SHH) couldn't be obtained which is needed to be done by regression analysis as well as the prediction model of DMY in the future study.

• Spatial Information Research
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• v.12 no.2
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• pp.151-163
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• 2004

This study aims to develop a standard protocol by which users can exchange data information efficiently between KOSDIC and GIS. The ultimate goal from this study is to propose a data exchange format using KOSIC. The background of this study is that GIS industry sector is vigorously developing linked to spatial information construction such as UIS, LIS, EIS, AM/FM through supporting by regional government institution. According to do, the format of data transformation becomes major issue. GIS data can easily transfer to CAD version but when CAD data convert to GIS format (DWG->DXF->SHP) cannot get high success rate due to errors and problems. In addition, CAD data feature is made from oriental structure which is made of point and line faeture so that GIS system is impossible to data transformation. In the reason, the biggest problem is discordance of symbolization in integration and standard of data format. By investigation the usability of this study, conclusion are drawn efficient alternative plan in data transformation. Though this wort we will be able to provide a better transformation of data conversion between CAD format and GIS format. Accordingly, it is of important to enrich the integrated construction information infrastructure, which accommodates ever-changing constructions environment.

• Journal of the Korea Institute of Military Science and Technology
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• v.14 no.2
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• pp.313-320
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• 2011

SPH(Smoothed Particle Hydrodynamics) is a gridless Lagrangian technique that is useful as an alternative numerical analysis method used to analyze high deformation problems as well as astrophysical and cosmological problems. In SPH, all points within the support of the kernel are taken as neighbours. The accuracy of the SHP is highly influenced by the method for choosing neighbours from all particle points considered. Typically a linked-list method or tree search method has been used as an effective tool because of its conceptual simplicity, but these methods have some liability in anisotropy situations. In this study, convex hull algorithm is presented as an improved method to eliminate this artifact. A convex hull is the smallest convex set that contains a certain set of points or a polygon. The selected candidate neighbours set are mapped into the new space by an inverse square mapping, and extract a convex hull. The neighbours are selected from the shell of the convex hull. These algorithms are proved by Fortran programs. The programs are expected to use as a searching algorithm in the future SPH program.

• Proceedings of the KSRS Conference
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• v.2
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• pp.896-898
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• 2006

Geological symbols are used for describing geological information. But it's not ease to represent them in commercial GIS s/w, because of their complexity and diversity. This study aims at developing the geological symbol mapping tool for representing geological symbol on user's geological information. Geological symbol mapping too is a web application which can handle SHP format and map geological symbols based on user's requests. It manages geological symbols and mapping codes and symbols are mapped within the geological boundary according to the corresponding non-spatial field that is a mapping code. The system has functions to upload a user's GIS file, and download the converted image file which is mapped geological patterns. The system displays converted images to be check mapping results. Because the symbols are simple bitmap files, user(system manager) can design and apply them rapidly without considering specific commercial S/W. Thus, it is expected that this system plays an important role to disseminate geological standards such as geological symbols. And the results of this study can be used for developing global geological symbols and applying them easily

• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2003.05a
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• pp.37-42
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• 2003

Fundamental Study for optimizing ship hull form using SQP(sequential quadratic programming) method in a resistance point of view is presented. The Wigley hull is used as an initial hull and numerical calculations are carried out according to various froude numbers. To obtain the ship resistance the wave resistance is evaluated by a Rankine source panel method with nonlinear free surface conditions and the ITTC 1957 friction line is used to predict the frictional resistance coefficient. The geometry of a hull surface is represented and modified by B-spline surface patch. The displacement and the waterplane transverse 2nd moment of inertia of the hull is fixed during the optimization process. And the shp design program called EzHULL is used to draw the lines of the optimized hull form to perform the model test.