• Imaging Science in Dentistry
• /
• v.52 no.3
• /
• pp.239-244
• /
• 2022

Purpose: Despite the proliferation of numerous morphometric and anthropometric methods for sex identification based on linear, angular, and regional measurements of various parts of the body, these methods are subject to error due to the observer's knowledge and expertise. This study aimed to explore the possibility of automated sex determination using convolutional neural networks(CNNs) based on lateral cephalometric radiographs. Materials and Methods: Lateral cephalometric radiographs of 1,476 Iranian subjects (794 women and 682 men) from 18 to 49 years of age were included. Lateral cephalometric radiographs were considered as a network input and output layer including 2 classes(male and female). Eighty percent of the data was used as a training set and the rest as a test set. Hyperparameter tuning of each network was done after preprocessing and data augmentation steps. The predictive performance of different architectures (DenseNet, ResNet, and VGG) was evaluated based on their accuracy in test sets. Results: The CNN based on the DenseNet121 architecture, with an overall accuracy of 90%, had the best predictive power in sex determination. The prediction accuracy of this model was almost equal for men and women. Furthermore, with all architectures, the use of transfer learning improved predictive performance. Conclusion: The results confirmed that a CNN could predict a person's sex with high accuracy. This prediction was independent of human bias because feature extraction was done automatically. However, for more accurate sex determination on a wider scale, further studies with larger sample sizes are desirable.

• Korean journal of applied entomology
• /
• v.61 no.1
• /
• pp.35-50
• /
• 2022

In this review, the compositions of the sex pheromones of 22 lepidopteran species were analyzed and briefly arranged by comparing the research results conducted in different locations worldwide. These compositions can be used as monitoring tools for pests of rice, maize, leguminous crops, perilla, and sesame in Korea. The sex pheromone compounds and those compositions of four species were successfully identified within Korean populations. The optimal compositions of sex pheromones of three other species were selected through behavioral studies using synthetic chemicals of the known pheromone compounds in laboratories and fields, without chemical identification processes in the sex pheromone compounds of Korean populations. For a few species, the roles of the minor components of the sex pheromone volatiles should be re-assessed for use in the development of optimal monitoring lures.

• Asian-Australasian Journal of Animal Sciences
• /
• v.16 no.5
• /
• pp.650-654
• /
• 2003

The accuracy of Polymerase chain reaction (PCR) assay in sexing of sheep embryos was assessed in this study. A total of 174 ovine embryos produced in vitro at different stages of development (2, 4-8 cell stages, morula and blastocyst) were sexed. The universal primers (P1-5EZ and P2-3EZ) used in this assay amplified ZFY/ZFX-specific sequences and yielded a 445 bp fragment in both sexes. Restriction enzyme analysis of ZFY/ZFX-amplified fragments with Sac I exhibited polymorphism between sexes, three and two fragments in males and in females, respectively. For verification of accuracy, blood samples of known sex were utilized as positive controls in each test. The mean percentages of sex identification by this method at 2 cell, 4-8 cell, morula and blastocyst were $73.00{\pm}5.72$, $89.77{\pm}3.79$, $3.33{\pm}8.08$ and $79.6{\pm}9.09$, espectively with the over all male to female ratio of 1:0.87. It is concluded that the ZFY/ZFX based method is highly reliable for the sexing of sheep embryos.

• Journal of Oral Medicine and Pain
• /
• v.20 no.2
• /
• pp.497-513
• /
• 1995

Human genomic Deoxyribonucleic acid(DNA) was extracted from teeth by boiling, salting-out, phenol, boiling-phenol methods. The author compared DNA concentration and its purity, the accuracy of sex determination and the results of the D1S80 locus detection among above 4 methods. The following results were obtained : 1. DNA concentration was the highest in pulp with salting-out method and DNA purity was higher in pulp with salting-out and phenol methods than other 2 methods. 2. Sex determination was possible using of the pulp and the dentin of the teeth with four methods but, it was impossible in the enamel and some pulp with boiling method. 3. Amplification of D1S80 locus occurred from pulp and dentin with salting-out, phenol, and boiling-phenol methods. 4. There are no differences among the amplification of X-Y homologus amelogenin gene by application of 4 methods and salting-out, phenol methods efficiently makes available to amplification of D1S80 locus. From the investigation DNA extraction, sex determination, amplification of D1S80 locus was successfully accomplished with salting-out, phenol, boiling-phenol methods Therefore above 3 methods are available and applicable as forensic odontology for individual identification.

• Journal of Life Science
• /
• v.15 no.1 s.68
• /
• pp.45-48
• /
• 2005

The aim of this study was to construct a correct pedigree of miniature horses (MH). The sex of MH was detected by PCR amplification of the sex determining region of the Y chromosome gene (SRY) prior to parentage testing. Ten random MH samples for parentage testing were genotyped by using 16 micro satellite markers. Since the SRY band (430 bp) was detected in horses No.1, 2, 6, 7, 8, 9, 10, these are male. However, the DNA segment was not identified in horses No.3, 4, and 5, which therefore are female. After genotyping, parentage testing was performed according to Mendelian fashion and International Society for Animal Genetics (ISAG) guideline. Of the 10 MH, 3 were qualified by the compatibility of 16 markers according to Mendelian fashion in the present DNA typing for parentage verification. These results can provide basic information for developing parentage verification and an individual identification system in MH.

• Journal of Oral Medicine and Pain
• /
• v.44 no.4
• /
• pp.169-173
• /
• 2019

Purpose: To determine whether the morphological features of the palatal rugae are associated with sex and age in children and adolescents. Methods: A total of 300 diagnostic models of the palatal rugae of children and adolescents were collected. The models were classified into male and female and<13- and ≥13-year-old groups. The palatal rugae pattern, and the number and length of palatal rugae plicae, were analyzed. Results: The number of palatal rugae plicae was higher in females than in males, however, the difference was negligible. In the group aged 13 years or more, the number was higher in the male group on the left side. There was no association between the number of palatal rugae plicae and age group. The type I pattern was the most common in both males and females. The length of palatal rugae plicae was greater in males than in females. There was no association between the length of palatal rugae plicae and age group. Conclusions: The number and length of palatal rugae were associated with sex, but the morphological features of the palatal rugae could not distinguish between children and adolescents. These findings suggest that the palatal rugae have limited value for identification of individuals.

• Korean Journal of Plant Resources
• /
• v.27 no.3
• /
• pp.236-241
• /
• 2014

A sex-linked random amplified polymorphic DNA (RAPD) marker was identified from Asparagus officinalis L. and was converted into a sequence-characterized amplified regions (SCAR) marker for the large-scale screening of male and female plants. A total of 100 arbitrary decamer oligonucleotide primers were used for the RAPD analysis. Among them, the primer UBC347 amplified one female-specific 400 base pair DNA. Subsequently, the amplified RAPD fragment was cloned and sequenced. The fragment was abundant in AT and shared sequence homology with retrotransposon elements. On the basis of the sequence obtained, a pair of SCAR primer was designed. The amplification product, named F400, was the same size as the respective RAPD fragment from which it was derived. The F400 SCAR marker resulted to be female-specific in the three asparagus varieties tested in this study. This SCAR marker can be used for an early and rapid identification of female and male plants during breeding programs of asparagus.

• Journal of the Korean Society of Clothing and Textiles
• /
• v.6 no.2
• /
• pp.41-48
• /
• 1982

The purposes of this study were (1) to investigate the relationships among the variables of unisex attitudes toward clothing, sex-role identification, hobbies and preferences, age, and educational level and (2) to find out any significant differences in the acceptance of unisex clothing between rural and urban residents and between males and females. The following results were obtained by interviewing 127 females and 117 males. (1) The acceptance of unisex clothing for females was generally higher than for males. (2) Younger and more educated persons had higher tendency in the acceptance of unisex clothing. (3) Persons with higher unisex attitude toward sex-role identification showed higher acceptance of unisex clothing and persons with higher unisex attitude toward hobbies and preferences seemed to have higher acceptance of unisex clothing for females. (4) Females showed higher tendency in the acceptance of unisex clothing for females than for males. (5) No significant differences were found in the acceptance of unisex clothing between rural and urban residents.

• The Journal of the Society of Korean Medicine Diagnostics
• /
• v.17 no.1
• /
• pp.29-44
• /
• 2013

Objectives This study is aimed at assessing the reliability of the Pattern identification questionnaire (PIQ) developed by Korea Institute of Oriental Medicine and examining the validity of the PIQ by comparing the pattern identification scores of different groups. Methods We conducted a survey of 258 participants (79 teachers and 179 graduate students at one School of Korean Medicine) using self-reported questionnaire and all the samples were retested. The test-retest reliability was assessed by Kappa coefficient(${\kappa}$) and Pearson correlation coefficient. Also we compared the differences in pattern identification scores according to sex, age and occupation. Results 1. One of 116 questions are impossible to calculate; 22 of them (18.97%) scored under 0.4 in ${\kappa}$; 90(77.59%) ranged from 0.4 to 0.8 in ${\kappa}$; and three questions (3.58%) scored 0.8 or over in ${\kappa}$. 2. Pearson correlation coefficients between test score and retest score of all pattern identification items are 0.4 or over. 3. The mean score for pattern identification in women was generally higher than that in men, particularly in patterns of blood-deficiency, blood-stasis, yang-deficiency and kidney disease. 4. The mean score for pattern identification in the graduate student group was generally higher than that in the teacher group. Conclusion In test-retest reliability, the PIQ showed relatively high reliability. The mean pattern identification score showed differences in regards to retaining knowledge about Korean medicine. Therefore, future research involving modification of questionnaire items and confirming the validity of this questionnaire is required.

• Journal of dental hygiene science
• /
• v.22 no.4
• /
• pp.249-255
• /
• 2022

Background: Sexual dimorphism is important for sex determination in the field of forensics. However, sexual dimorphism is commonly assessed using cone beam computed tomography (CBCT) rather than three-dimensional (3D) modeling software; therefore, studies using a more accurate measurement approach are necessary. This study assessed the sexual dimorphism of the MS using a 3D modeling program to obtain information that could contribute to the fields of surgery and forensics. Methods: The CBCT data of 60 patients (age, 20~29 y; 30 males and 30 females) admitted to the Department of Orthodontics at the Dankook University School of Dentistry were provided in Digital Imaging and Communications in Medicine (DICOM) format. The left MS and right MS were modeled based on the DICOM files using the Mimics (version 22; Materialise, Leuven, Belgium) 3D program and converted to stereolithography (STL) files used to measure the width, length, and height of the MS, infraorbital foramen (IOF), right MS, and left MS. The average of three repeated measurements was calculated, and a reliability test was performed to ensure data reliability (Cronbach's α=0.618). A canonical discriminant analysis was performed using a standard approach (left: Box's M=0.096; right: Box's M=0.115). Results: Males had greater values for all parameters (MS width, MS length, MS height, IOF, right MS, left MS) than females. The discriminant analysis identified six independent variables (MS width, MS height, MS length, IOF, right MS, left MS) that could identify sex. The left MS and right MS correctly identified the sex of 81.7% and 71.7% of the patients, respectively, with the left MS having higher accuracy. Conclusion: This study confirmed that, for Korean individuals, the left MS has a better ability to identify sex than the right MS. These results may contribute to sex identification in the fields of surgery and forensics.