• 제목/요약/키워드: SEPT6

검색결과 143건 처리시간 0.024초

SEPT12 Interacts with SEPT6 and This Interaction Alters the Filament Structure of SEPT6 in Hela Cells

  • Ding, Xiangming;Yu, Wenbo;Liu, Ming;Shen, Suqin;Chen, Fang;Wan, Bo;Yu, Long
    • BMB Reports
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    • 제40권6호
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    • pp.973-978
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    • 2007
  • Septins are a family of conserved cytoskeletal GTPase forming heteropolymeric filamentous structure in interphase cells, however, the mechanism of assembly are largely unknown. Here we described the characterization of SEPT12, sharing closest homology to SEPT3 and SEPT9. It was revealed that subcelluar localization of SEPT12 varied at interphase and mitotic phase. While SEPT12 formed filamentous structures at interphase, it was localized to the central spindle and to midbody during anaphase and cytokinesis, respectively. In addition, we found that SEPT12 can interact with SEPT6 in vitro and in vivo, and this interaction was independent of the coiled coil domain of SEPT6. Further, co-expression of SEPT12 altered the filamentous structure of SEPT6 in Hela cells. Therefore, our result showed that the interaction between different septins may affect the septin filament structure.

한국의 동물과 사람에서 분리한 Salmonella enteritidis의 phage types (Phage types of Salmonella enteritidis isolated from animal and human in Korea)

  • 우용구;박미선;우승룡;김봉환;김재학
    • 대한수의학회지
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    • 제40권3호
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    • pp.515-524
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    • 2000
  • The present study was conducted to investigate the phage types(PT) of Salmonella enteritidis(SE) isolated from animals and human patients in Korea during the period 1993-1999. SE were isolated from chickens including the layer and broiler, duck, rat, swine and human patients in nationwide scale. A total of 231 non-human and 14 human SE strains were phage typed and 80.4%(197/245) of SE isolates tested could discriminate as a ten SEPTs including the RDNC by the 10 standard phages, which were brought from the Central Public Health Laboratory in England. In analysing of phage types both animal and human SE isolates, SEPT 4(55.4%) was the most prevalent in domestic animals including the layer(39.3%) and broiler(64.5%), duck(55.5%), and swine(75.0%) since 1993. About 57% of the S enteritidis from human was SEPT 4. Along with SEPT 4, SEPT 1, SEPT 6a, SEPT 7 and SEPT 7a var, SEPT 9b, SEPT 15, SEPT 21b and SEPT 22 were also found from animal and human SE isolates. SEPT 4(40.0%) was isolated from frozen chicken meats imported from China along with SEPT 7 and 7a var strains in this study. In conclusion, SEPT 4 was the most predominant SEPT in our country regardless of animal or human hosts including the Che-Ju island and almost exclusively found in domestic poultry(layer and broiler). During the review period, SEPT 4 has already been spreading continually among the animal hosts since 1993.

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SEPT6 in Rat Hippocampal Neurons

  • Cho, Sun-Jung;Walikonis, Randall S.;Moon, Il-Soo
    • 한국생명과학회:학술대회논문집
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    • 한국생명과학회 2006년도 제47회 학술심포지움 및 추계국제학술대회
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    • pp.43.2-43.2
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    • 2006
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Analysis of polychlorinated biphenyls in shellfish collected from a market

  • Lee, Ga-Jeong;Moon, Ji Yong;Lee, Eun-Hwa;Ha, Hye-Sook;Jeong, Gi Ho
    • 분석과학
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    • 제18권6호
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    • pp.529-534
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    • 2005
  • We collected seven species of shellfish; all originating from the southern coastal areas of Korea, from a market every three months from Dec. 2001 to Sept. 2002, and determined the total polychlorinated biphenyl(PCB) levels by the sum of 26 individual congener levels. A GC-ECD system was applied for identification and quantification of these PCB congeners. Mussel showed the highest level in Sept. 2002 at 34.5 ng/g dry weight(d.w.). All species except mussel showed the lowest total PCB level in Dec. 2001 and their levels in tissue ranged from 0.6 to 5.5 ng/g d.w. The total PCB levels ranged from 0.8 to 17.3 ng/g in Mar. 2002, 2.2 to 9.5 ng/g in June 2002, and 1.8 to 34.5 ng/g d.w. in Sept. 2002. The principal congener group was penta-CBs, which accounted for 32% of the total PCBs, followed by hexa-CBs at 23%, and tetra-CBs at 21%.

우리나라의 가금과 환경에서 분리한 Salmonella species의 특성 (Characteristics of Salmonella species isolated from domestic poultry and environmental samples in Korea)

  • 우용구;이희수;이영주;강민수;김봉환;김재학
    • 대한수의학회지
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    • 제40권3호
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    • pp.505-514
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    • 2000
  • This study was conducted to investigate the isolation prequency, serotypes, and related epidemiological properties of 341 Salmonella spp from domestic poultry and environmental samples during the period 1993-1995. A total of 1,918 samples was collected during the three years period in nationwide. Most of Salmonella spp were isolated from the intestinal contents of poultry, especially cecal(46.0%) and rectal(35.8%) contents. Among the tested samples, rat(28.5 %) was the most predominant Salmonella reservoirs and followed by duck(24.8%), broiler(18.8%), layer(14.8%) and feed(7.1%), in order. More than twelve Salmonella serovars were identified among the 341 Salmonella isolates. The most prevalent serotypes isolated from non-human sources were S enteritidis (22.3%) and S pullorum (21.9%), S muenchen (13.9%), S typhimurium (12.6%), S gallinarum, S meleagridis, S heidelberg, and S senftenberg were followed, in order. In layer chickens, S pullorum (26.0%) was the most predominant serotype but S muenchen (33.0%) was in broiler chickens, S enteritidis (28.4%) was in ducks, and S typhimurium (60.0%) was in rats, respectively. As a results, S enteritidis was identified as the most prevalent serotype in non-human Salmonella isolates in Korea during the period 1993-1995. A preliminary study on the phage typing of 19 S enteritidis selected from the nationwide scale was shown that S enteritidis phage type(PT) 4 was the most predominant PT, and SEPT 1, SEPT 6a, SEPT 7 and SEPT 7a variant were also found in the same period.

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시판 살오징어(Todarodes pacificus) 간장 및 생식소의 일반성분과 지방산 조성 (The Proximate and Fatty Acid Compositions of the Liver and Gonads of Commercial Common Squid Todarodes pacificus)

  • 문수경;박혜진;정보영;김인수
    • 한국수산과학회지
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    • 제51권6호
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    • pp.656-666
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    • 2018
  • To evaluate the optimal time for extracting lipids from the viscera of the common squid Todarodes pacificus the proximate and fatty acid compositions of the liver and gonads of commercial squid were analyzed according to sex for 6 months (Jul. to Sept. and Nov. to Jan.). The body and liver weights of the squid were larger in females than in males, and were larger from Nov. to Jan. than from Jul. to Sept. in both sexes. The average lipid contents in the livers of female and male squid were also higher from Nov. to Jan (22.9% and 24.9%, respectively) than from Jul. to Sept. (11.7% and 17.5%, respectively). The average lipid contents of the ovaries and testes were 5.16% and 1.76%, respectively, and changed little over the 6 months. The percentage of n-3 polyunsaturated fatty acids (eicosapentaenoic acid + docosahexaenoic acid) was higher in the gonads (44.8-49.1%) than in the liver (36.3-37.6%). These results suggest that Nov. to Jan. is the best time to extract lipids from the liver, whereas there was no difference in the efficacy of lipid extraction from the gonads between the two seasons.

Changes in Oil, Tannin, Total Sugar Contents and Yield after Flowering in Peanut

  • Lee, Sung-Woo;Park, Chang-Hwan;Kang, Chul-Whan;Kim, Sok-Dong
    • 한국작물학회지
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    • 제44권2호
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    • pp.159-162
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    • 1999
  • This study was carried out to determine the optimum harvest date of unshelled immature peanuts and dried kernels from 60 to 120 days after flowering. Fresh pod yield of spanish-type variety, 'Shaedl-tangkong(SD)' reached a peak of 9,140kg/ ha at 70 (days after flowering(Aug. 13) while that of virginia-type variety, 'Daepoongtangkong(DP)' reached a peak of 8,820kg/ha at 90 days(Sept. 11) after flowering. SD and DP showed maximum fresh kernel yield of 6,090 and 6,470kg/ha at 90 days after flowering (Sept. 11), respectively, while dry kernel yield reached a peak of 3,300 and 3,720kg/ha at 110 days(Oct. 1), respectively. Oil content of SD and DP were the highest at 90 days and 100 days after flowering, respectively and the oil content of two varieties increased rapidly from 60 to 90 days. Tannin content of the seed hull of SD increased continuously until 110 days after flowering while that of DP maximized at 100 days. The tannin content of the two varieties increased rapidly from 60 to 100 days. Total sugar of SD and DP showed highest content at 60 days and 70 days after flowering, respectively and suger content decreased very rapidly until 80 days and after that sugar content kept nearly constant. Oil, tannin and total sugar content of spanish-type SD were higher than those of virginia-type DP.

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주요(主要) 과수(果樹)에서 발생(發生)하는 진딧물의 종류(種類)와 발생소장(發生消長)에 관(關)한 연구(硏究) (Colonizing Aphid Species and Their Seasonal Fluctuations on Some Fruit Trees in Suweon)

  • 김석환;이순원;김인수;이문홍
    • 한국응용곤충학회지
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    • 제25권4호
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    • pp.209-213
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    • 1986
  • 사과, 복숭아, 배나무등을 가해(加害)하는 진딧물의 종류(種類)와 우점종(優占種) 및 발생소장(發生消長)을 $'82{\sim}'84$년(年)까지 농기연(農技硏)과 원예시험장(園藝試驗場)의 살충제(殺蟲劑) 무살포(無撤布) 과수원(果樹園)에서 조사(調査)한 결과(結果)는 다음과 같다. 1. 사과나무를 가해(加害)하는 진딧물은 사과혹진딧물과 조팝나무진딧물로 우점종(優占種)은 $4{\sim}7$월(月)에 사과혹진딧물, $8{\sim}10$월(月)에 조팝나무진딧물로 시기(時期)에 따라서 변화(變化)하였고, 사과혹진딧물은 6월상순(月上旬), 8월상순(月上旬), 10월상순(月上旬)에, 조팝나무진딧물은 6월상순(月上旬), 8월중순(月中旬), 9월하순(月下旬)에 최대밀도(最大密度)를 형성(形成)하였다. 그 이후(以後)에는 복숭아가루진딧물진딧물이었으며, 복숭아혹진딧물은 5월하순(月下旬)$\sim$6월상순(月上旬), 복숭아가루진딧물은 6월중순(月中旬)과 10월중순(月中旬)에 최대밀도(最大密度)를 형성(形成)하였다. 3. 배나무를 가해(加害)하는 진딧물은 배나무동글밑진딧물, 자주동글진딧물, 조팝나무진딧물등으로 우점종은 4월(月)$\sim$6월(月)과 10월(月)에는 배나무동글밑진 2. 복숭아나무를 가해(加害)하는 진딧물은 복숭아혹진딧물, 복숭아가루진딧물, 복숭아잎혹진딧물등으로 우점종(優占種)은 초봄에는 복숭아혹진딧물이었고 딧물, $7{\sim}9$월(月)에는 조팝나무진딧물이였으며, 배나무동글밑진딧물은 5월하순(月下旬)과 10월하순(月下旬)에 조팝나무진딧물은 6월상순(月上旬)과 9월상순(月上旬)에 최대밀도(最大密度)를 형성(形成)하였다.

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체질정보은행 구축에 관한 연구 (Study on the Construction for Constitution Information Bank)

  • 이시우;이수경;주종천;유현희;장은수
    • 동의생리병리학회지
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    • 제21권6호
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    • pp.1586-1589
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    • 2007
  • This study aims to construct Constitution Information Bank to objectification of Sasang Constitution Diagnosis and Treatment. 422 cases clinically confirmed their Constitution by experts are collected retrospectively and 219 cases for constitution-family study are also collected in Constitution Information Bank from Nov. 2006 to Sept. 2007. The information include their physique, appearance, character, temper, symptom and treatment records. Their DNA cell lines also included in Constitution Information Bank for constitution-genome study. Constitution Information Bank is composed of constitution-clinical information -physique, appearance, character, temper, symptom and treatment records- and biological information -DNA genotyping data-. Total of 550 cases are collected in Constitution Information Bank from Nov. 2006 to Sept. 2007. Among these, 219 cases are for constitution-family study, especially, 74 cases are collected from Han's large family.

맥후작 참깨의 개화와 종실등숙 특성 (Flowering and Seed Maturation of Sesame Cropped After Winter Barley)

  • 이호진;윤진일;권용웅
    • 한국작물학회지
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    • 제25권1호
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    • pp.66-71
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    • 1980
  • 참깨의 만기재배시 개화 및 등숙특성을 알고자 품종 $\ulcorner$수원 9호$\lrcorner$를 6월 19일과 7월 3일 2회에 걸쳐 1kg/10a의 파종량으로 휴입조파하였다. 개화기에 각 파종익별로 5주를 선정하여 절위별 개화진전 및 성숙과정과 엽의 황화 및 삭의 열개를 조사하였으며, 7/3 파종구에서는 개화시부터 5일 간격으로 매회40주씩 개화절위에 표식하여 이들로부터 5주씩 선정 5일 간격으로 표식된 절위의 삭에 대하여 길이, 굵기, 종실수, 삭 및 종실의 건물중 및 삭의 수분함량을 조사하였으며, 그 결과는 다음과 같다. 1. 최초 개화는 파종후 37~40일 부터 시작되었고 절위별 개화완료 소요 일수는 6/19 파종구에서 1.78일, 7/3 파종구에서 1.56일로 파종이 늦어짐에 따라 단축되었다. 2. 삭의 길이 및 굵기는 개화후 10 일경에 완성되었으며 종실수는 15 일경에 결정되었으나, 종실중은 개화후 15일부터 증가하기 시작하여 35 일경에 최대에 달하였다. 3. 평균기온 $20^{\circ}C$ 이하 및 엽의 50%가 황화되었을 때부터 종실중의 증가가 중지되었다.

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