• Journal of Microbiology and Biotechnology
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• v.29 no.4
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• pp.651-657
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• 2019

Although smallpox was eradicated in 1980, it is still considered a potential agent of biowarfare and bioterrorism. Smallpox has the potential for high mortality rates along with a major public health impact, eventually causing public panic and social disruption. Passive administration of neutralizing monoclonal antibodies (mAbs) is an effective intervention for various adverse reactions caused by vaccination and the unpredictable nature of emerging and bioterrorist-related infections. Currently, vaccinia immune globulin (VIG) is manufactured from vaccinia vaccine-boosted plasma; however, this production method is not ideal because of its limited availability, low specific activity, and risk of contamination with blood-borne infectious agents. To overcome the limitations of VIG production from human plasma, we isolated two human single-chain variable fragments (scFvs), (SC34 and SC212), bound to vaccinia virus (VACV), from a scFv phage library constructed from the B cells of VACV vaccine-boosted volunteers. The scFvs were converted to human IgG1 (VC34 and VC212). These two anti-VACV mAbs were produced in Chinese Hamster Ovary (CHO) DG44 cells. The binding affinities of VC34 and VC212 were estimated by competition ELISA to $IC_{50}$ values of $2{\mu}g/ml$ (13.33 nM) and $22{\mu}g/ml$ (146.67 nM), respectively. Only the VC212 mAb was proven to neutralize the VACV, as evidenced by the plaque reduction neutralization test (PRNT) result with a $PRNT_{50}$ of ~0.16 mg/ml (${\sim}1.07{\mu}M$). This VC212 could serve as a valuable starting material for further development of VACV-neutralizing human immunoglobulin for a prophylactic measure against post-vaccination complications and for post-exposure treatment against smallpox.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.30 no.6D
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• pp.541-552
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• 2010

The light rail transit (LRT) having bus lines as subsystem is being constructed or planned in the suburban area of metropolitans and medium size cities. However, there is difficulty in establishing the service coverage (SC) of the LRT because the LRT is a completely new transit mode in Korea. The purpose of this study is to provide the basic data and techniques to be used for establishing the SC of the future LRT by understanding the SC characteristics of buses and subways and building models to estimate the walking distances of their users. Busan City is selected as the study city and the SC's of buses and subways are surveyed simultaneously. A total of 9 variables for 82 stations are collected and the cluster analysis is conducted about the variables. The station areas are divided to three types of CBD (Central Business District), sub-CBD and regional center based on the analysis. A station in each area is selected as the study station. At the walking distance (WD) analysis for each mode, the 80 percentile WD of the subway is 672 m and that of the bus is 472 m. In comparing the SC's of both modes by the type of station areas, there are not significant differences between the SC's of sub-CBD and regional center except CBD. At analysis of the relationship between the personal attributes and the WD, for subway users the WD of female is longer than that of male and apartment residents use subway more positively than single house residents do. For the models to estimate the walking distances, the simple regression models were built employing the income as independent variable by dividing the stations into CBD abd non-CBD stations.

• Korean Journal of Food Science and Technology
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• v.38 no.5
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• pp.642-647
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• 2006

Yeast selection and the sterilization methods of strawberry juice were optimized for making strawberry wine. In addition, changes in the physicochemical characteristics of the wine during its fermentation were estimated. Maehyang and Yukbo cultivars of strawberry were tested for wine making; they contained 9.8 and 9.3% soluble solids and 0.59 and 0.58% titratable acidities, respectively. Among six yeasts tested, the Wg-15 and Sc-51 strains were selected based on the alcohol yield in the strawberry wine. Alcohol and soluble solid contents following heat treatment ($85^{\circ}C$, 10 min) or $K_2S_2O_5$ (200 ppm) treatment for sterilization were 7.10-7.20% and 5.60-5.80%, respectively, and no differences were observed between the Wg-15 and the Sc-51 strains. However, the flavor of wine produced following heat treatment was slightly better than that following $K_2S_2O_5$ treatment. The greatest amounts of alcohol were produced after 2 days of fermentation at $26^{\circ}C$. The alcohol content in wines produced with 12, 14, and 16% sugar content in the initial stages were 5.1, 6.0-6.2, and 7.5-7.7%, and the soluble solid contents were 3.9-4.3, 4.1-4.3, and 5.0-5.3%, respectively; no significant differences were observed between the Wg-15 and the Sc-51 yeast strains. For making strawberry wine, we proposed that the sugar content of Maehyang or Yukbo cultivars be adjusted to 16% soluble solids in the initial stages with heat treated at $85^{\circ}C$ for 10 min and fermentation with the Wg-15 or Sc-51 yeast strains at $26^{\circ}C$ for 8 days.

• Tunnel and Underground Space
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• v.22 no.4
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• pp.266-275
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• 2012

In this study, a method is devised to implement a supercritical $CO_2$ ($scCO_2$) injection environment on a laboratory scale and to investigate the effects of $scCO_2$ on the properties of rock specimens. Specimens of shale and sandstone normally constituting the cap rock and reservoir rock, respectively, were kept in a laboratory reactor chamber with $scCO_2$ for two weeks. From this stage, a chemical reaction between rock surface and the $scCO_2$ was induced. The effect of saline water was also investigated by comparing three conditions ($scCO_2$-rock, $scCO_2-H_2O$-rock and $scCO_2$-brine(1M)-rock). Finally, we checked the changes in the properties before and after the reaction by destructive and nondestructive testing procedures. The swelling of shale was a main concern in this case. The experimental results suggested that $scCO_2$ has a greater effect on the swelling of the shale than pure water and brine. It was also observed that the largest swelling displacement of shale occurred after a reaction with the $H_2O-scCO_2$ solution. The results of a series of the destructive and nondestructive tests indicate that although each of the property changes of the rock differed depending on the reaction conditions, the $H_2O-scCO_2$ solution had the greatest effect. In this study, shale was highly sensitive to the reaction conditions. These results provide fundamental information pertaining to the stability of $CO_2$ storage sites due to physical and chemical reactions between the rocks in these sites and $scCO_2$.

• Journal of Oriental Neuropsychiatry
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• v.3 no.1
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• pp.46-55
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• 1992

This clinical study aims to find out the effect of the fingertip's temperature and beverage-taking on the personality test by MMPI (Minnesota Multiphasic Personality Inventory). This is based on the sample survey on the 5 groups ; two or more-veined-handed group (15 persons), one-veined-handed group (22 persons), non-veined-hanede group(13 persons), beverage-taking group (41 persons) and beverage-non-taking group (14 persons). The result was obtained as follows ; 1. In the comparative examination on the two or mire-veined-haned group and the non-veined-handed group, there was significant difference of the temperature between the scale D and the scales of Mf, Pa, Pt, and Sc. 2. For the scales of Hs, D, Hy and the scales of Pd, Pa, Pt, Sc, the one-veined-handed group showed higher T-scores and lower fingertip's temperature than the non-veined-handed group without singnificant difference between them. 3. For the scales of D, Hy and the scales of Pd, Pa, Pt, Sc, the beverage-taking group showed higher T-scores and lower fingertip's temperature than the beverage-non-taking group without significant difference between them. As a result, I could find that low temperature on the fingertip, protruded vein on three knuckles(三關) of the finger and beverage-taking would cause the blood-extravasation(血瘀) and the lack of the transports in the spleen(脾不運化) and have an influence on Neurosis and Psychosis.

• Earthquakes and Structures
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• v.12 no.1
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• pp.109-118
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• 2017

A novel post-tensioned self-centering (SC) concrete beam-column connection with web friction devices has been proposed for concrete moment-resisting frames. This paper presents a probabilistic performance evaluation procedure to evaluate the performance of the self-centering concrete frame with the proposed post-tensioned beam-column connections. Two performance limit states, i.e., immediate occupancy (IO) and repairable (RE) limit states, are defined based on peak and residual story drift ratios. Statistical analyses of seismic demands revealed that the dispersion of residual drifts is larger than that of peak drifts. Due to self-centering feature of post-tensioning connections, the SC frame was found to have high probabilities to be recentered under the design basis earthquake (DBE) and maximum considered earthquake (MCE) ground motions. Seismic risk analysis was performed to determine the annual (50-year) probability of exceedance for IO and RE performance limit states, and the results revealed that the design objectives of the SC frame would be met under the proposed performance-based design approach.

• Journal of the Korean Society of Clothing and Textiles
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• v.42 no.1
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• pp.121-132
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• 2018

Recent beauty trends focus on suitability to individual features. A personal color system is a recent aesthetic concept that influences color make up and coordination. However, a personal color concept has several weaknesses. For example, type classification is qualitative and not quantitative because its measuring system is a sensory test with no industry standard of personal color system. A quantitative personal color type classification model is the purpose of this study, which can be a solution to above problems. This model is a kind of mapping system in a 3D Cartesian coordinate system which has own axes, Value, Saturation, and Yellowness. The cheek color of the individual sample is also independent variable and personal color type is a dependent variable. In order to construct the model, this study conducted a colorimetric survey on a 993 sampling frequency of Korean women in their 20s and 30s. The significance of this study is as follows. First, through this study, personal color system is established on quantitative color space; in addition, the model has flexibility and scalability because it consisted of independent axis that allows for the inclusion of any other critical variable in the form of variable axis.

• Food Science and Biotechnology
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• v.18 no.2
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• pp.312-316
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• 2009

Antioxidant-rich oils were extracted from different parts of sea buckthorn berry with supercritical $CO_2$ (SC-$CO_2$) and n-hexane. The functional components were analyzed and the extracts were screened for their potential as radical scavengers in 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), 2,2'-azinobis-3-ethylbenzotiazoline-6-sulphonic acid (ABTS), galvinoxyl systems. Minor differences were found in fatty acid composition of oils extracted by the two methods. Seed oil contains the highest content of tocopherols while pulp oil and whole berry oil possessed higher concentration of carotenoids. Whole berry oil, pulp oil, and seed oil extracted by SC-$CO_2$ showed 91.7, 90.9, and 93.5% radical scavenging activity (RSA) at 6 mg/mL towards DPPH and 74.3, 54.3, and 74.8% towards galvanoxyl radical at 10 mg/mL. The hexane-extracted oils showed similar scavenging ability. However, the oils obtained by hexane showed significantly higher RSA (p<0.05) than those obtained by SC-$CO_2$ while whole berry oil has the highest RSA towards ABTS among 3 oil samples.

• Food Science and Biotechnology
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• v.14 no.1
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• pp.6-10
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• 2005

Optimal extraction conditions such as pressures, temperatures, and modifiers on glycyrrhizin extraction from licorice were investigated using supercritical $CO_2\;(SC-CO_2)$ at 3 mL/min flow rate. Morphology of licorice tissue, after glycyrrhizin extraction, was examined by SEM, and absolute density ($g/cm^3$) measurement and glycyrrhizin content were determined by HPLC. Pure $SC-CO_2$ had no effect on glycyrrhizin extraction, but recovery of glycyrrhizin ($32.66{\pm}0.77%$) was enhanced when water was used as modifier. The highest recovery was $97.22{\pm}2.17%$ when 70% (v/v) aqueous methanol was added to 15% (v/v) $SC-CO_2$ at 50 MPa and $60^{\circ}C$. Under optimal extraction conditions, 30 MPa pressure and $60^{\circ}C$ heating temperature, glycyrrhizin recovery reached maximum ($102.67{\pm}1.13%$) within 60 min. Licorice tissue was severely damaged by excessive swelling, and absolute density of licorice residues was highest when aqueous methanol was used as a modifier.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.35 no.1A
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• pp.1-9
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• 2010

This paper offers performance analysis of selection decode and forward (DF) networks with differential modulation/demodulation for an arbitrary number of relays in independent but not identically distributed Rayleigh fading channels. We have shown that the selection DF protocol with differential modulation can achieve full diversity in both independent identically distributed (i.i.d.) and independent but not identically distributed (i.n.d.) Rayleigh fading channels, and the performance loss due to using non-coherent detection is not substantial. Furthermore, we study the impact of combining techniques on the performance of the system by comparing a system that uses selection combining (SC) to one that uses maximum ratio combining (MRC). Simulations are performed and show that they match exactly with analytic ones in high SNR regime.