• Title/Summary/Keyword: S1009A

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Functional Recovery Following the Transplantation of Olfactory Ensheathing Cells in Rat Spinal Cord Injury Model

  • Muniswami, Durai Murugan;Tharion, George
    • Asian Spine Journal
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    • v.12 no.6
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    • pp.998-1009
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    • 2018
  • Study Design: Olfactory ensheathing cells (OECs) from rat olfactory mucosa were cultured, characterized, and transplanted into a rat model of spinal cord injury (SCI). Purpose: To evaluate different doses of OECs in a rat model of SCI. Overview of Literature: SCI causes permanent functional deficit because the central nervous system lacks the ability to perform spontaneous repair. Cell therapy strategies are being explored globally. The clinical use of human embryonic stem cell is hampered by ethical controversies. Alternatively, OECs are a promising cell source for neurotransplantation. This study aimed to evaluate the efficacy of different doses of allogenic OEC transplantation in a rat model of SCI. Methods: OECs were cultured from the olfactory mucosa of Albino Wistar rats; these cells were characterized using immunohistochemistry and flow cytometry. Rats were divided into five groups (n=6 rats each). In each group, different dosage ($2{\times}10^5$, $5{\times}10^5$, $10{\times}10^5$, and >$10{\times}10^5$) of cultured cells were transplanted into experimentally injured spinal cords of rat models. However, in the SCI group, only DMEM (Dulbecco's modified Eagle's medium) was injected. Rats were followed up upto 8 weeks post-transplantation. The outcome of transplantation was assessed using the Basso, Beattie, Bresnahan (BBB) scale; motor-evoked potential studies; and histological examination. Results: Cultured cells expressed 41% of p75NTR, a marker for OEC, and 35% of anti-fibronectin, a marker for olfactory nerve fibroblast. These cells also expressed $S100{\beta}$ and glial fibrillary acid protein of approximately 75% and 83%, respectively. All the transplanted groups showed promising BBB scores for hind-limb motor recovery compared with the SCI group (p<0.05). A motor-evoked potential study showed increased amplitude in all the treated groups compared with the SCI. Green fluorescent protein-labeled cells survived in the injured cord, suggesting their role in the transplantation-mediated repair. Transplantation of $5{\times}10^5$ cells showed the best motor outcomes among all the doses. Conclusions: OECs demonstrated a therapeutic effect in rat models with the potential for future clinical applications.

Impact of Review Characteristics on Female Consumer Perceptions of Review Usefulness and Patronage Intent of Online Stores Hosting the Reviews

  • Hong, Heesook;Kim, Hye-Shin
    • Journal of the Korean Society of Clothing and Textiles
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    • v.40 no.6
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    • pp.994-1009
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    • 2016
  • Applying the S-O-R Model within an online context, a hypothesized model incorporates three review characteristics (perceived concreteness, exaggeration, and sufficient quantity of reviews) for apparel products in order to present their impact on consumer perceptions of review usefulness and consumer attitude toward and patronage intent for the online stores hosting the reviews. An online survey of Korean women (N=299) reported their experiences in purchasing apparel products online and reading apparel reviews on a regular basis. Testing of the hypothesized model showed the usefulness of reviews were determined by two review characteristics (S: perceived concreteness and sufficient quantity of reviews); however, the negative effect of exaggerated reviews were insignificant. In addition, the perceived usefulness of reviews (O-cognitive) hosted by an online store influenced online store attitude (O-affective) which subsequently led to online store patronage intent (R). This study systemically advances online retail literature by showing how the characteristics of online reviews (as a part of the online store environment) can influence attitude toward online stores and patronage intent for online stores. Long term relationships with consumers can be achieved through the building of mechanisms to enhance the perceived usefulness of reviews by employing the strategies of hosting concrete reviews and offering a sufficient quantity of reviews. This study addresses removes research gaps by testing an adapted the S-O-R Model that frames review information as an element of an online store environment using a large sample.

Analysis and Selection of Microsatellites Markers for Individual Traceability System in Hanwoo (한우 생산이력제에 활용 가능한 Microsatellite의 분석과 선발)

  • Lim, H.T.;Min, H.S.;Moon, W.G.;Lee, J.B.;Kim, J.H.;Cho, I.C.;Lee, H.K.;Lee, Y.W.;Lee, J.G.;Jeon, J.T.
    • Journal of Animal Science and Technology
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    • v.47 no.4
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    • pp.491-500
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    • 2005
  • To test applicability to the Hanwoo traceability system, twenty microsatellite markers were selected and analyzed. MSA, CERVUS, FSTAT, GENEPOP, API_CALC and PHYLIP software was employed serially to estimate heterozygosity, polymorphic information content, F-statistics, identity probability, exclusion probability and genetic distance. Eleven microsatellite markers(TGLA53, TGLA227, ETH185, TGLA122, BM4305, INRA23, ILSTS013, BMS1747, BM2113, BL1009, and ETH3) were selected based on their high heterozygosity values. Identity probability using these markers is one hundred times higher than when using StockMakersTM of Applied Biosystems. This indicates the selected microsatellite markers are appropriate and effective for use in the Hanwoo traceability system. Additionally, estimates of DA genetic distance and pairwise-FST can be utilized to identify genetic relationships between adjacent farms.

Comparison of the Vertical Data between Eulerian and Lagrangian Method (오일러와 라그랑주 관측방식의 연직 자료 비교)

  • Hyeok-Jin Bae;Byung Hyuk Kwon;Sang Jin Kim;Kyung-Hun Lee;Geon-Myeong Lee;Yu-Jin Kim;Ji-Woo Seo;Yu-Jung Koo
    • The Journal of the Korea institute of electronic communication sciences
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    • v.18 no.6
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    • pp.1009-1014
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    • 2023
  • Comprehensive observations of the Euler method and the Lagrangian method were performed in order to obtain high-resolution observation data in space and time for the complex environment of new city. The two radiosondes, which measure meteorological parameters using Lagrangian methods, produced air pressure, wind speed and wind direction. They were generally consistent with each other even if the observation points or times were different. The temperature measured by the sensor exposed to the air during the day was relatively high as the altitude increased due to the influence of solar radiation. The temporal difference in wind direction and speed was found in the comparison of Euler's wind profiler data with radiosonde data. When the wind field is horizontally in homogeneous, this result implies the need to consider the advection component to compare the data of the two observation methods. In this study, a method of using observation data at different times for each altitude section depending on the observation period of the Euler method is proposed to effectively compare the data of the two observation methods.

The unbalance compensation methode of PWM converter in a Utility interactive Photovoltaic Generation System (계통연계형 태양광발전 시스템의 불평형 보상기법)

  • Lee, J.H.;Shin, C.J.;Jeon, K.Y.;Bak, C.W.;Chun, J.Y.;Lee, S.C.;Oh, B.H.;Chung, C.B.;Han, K.H.
    • Proceedings of the KIEE Conference
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    • 2006.07b
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    • pp.1008-1009
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    • 2006
  • There is an application which uses smoothing reacter connecting each of the phases in order to make the system smaller comparison with an application using the insulating transformer in the utility interactive photovoltaic generation system using the PWM converter. In this case, there might be the occurence of the unbalance of output voltage, by the difference of smoothing reactor, connected on each of the lines. Therefore this study proposes that the method which maintains the balance and compensates the unblance which occured by the different volume of the smoothing reactor connected on each of the lines.

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A Study on the Propogation Velocity of Biological Action Potential (생체의 자극 전도속도에 관한 연구)

  • Che, Gyu-Shik;Kwon, Yong-Chan
    • Journal of Advanced Navigation Technology
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    • v.14 no.6
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    • pp.1002-1009
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    • 2010
  • I made the axon to the electrical transmission model and then constructed electrical equivalent model using Kirhhoff's current law and voltage law in this paper. I calculated various axon parameters in order to analyze the electrical potential hehavior versus minute distance chang of axon. The transmission velocity of unmyelinated nerve is proportional to square root of axon diameter, while that of myelinated nerve is directly proportional to its diameter. Because the transmission of myelin sheath is independent of voltage unlike unmyelinated sheath, the Hodgkin-Huxley model across the membrane is not so precise.

LC-MS/MS Analysis of Surface Layer Proteins as a Useful Method for the Identification of Lactobacilli from the Lactobacillus acidophilus Group

  • Podlesny, Marcin;Jarocki, Piotr;Komon, Elwira;Glibowska, Agnieszka;Targonski, Zdzislaw
    • Journal of Microbiology and Biotechnology
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    • v.21 no.4
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    • pp.421-429
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    • 2011
  • For precise identification of a Lactobacillus K1 isolate, LC-MS/MS analysis of the putative surface layer protein was performed. The results obtained from LTQ-FT-ICR mass spectrometry confirmed that the analyzed protein spot is the surface layer protein originating from Lb. helveticus species. Moreover, the identified protein has the highest similarity with the surface layer protein from Lb. helveticus R0052. To evaluate the proteomic study, multilocus sequence analysis of selected housekeeping gene sequences was performed. Combination of 16S rRNA sequencing with partial sequences for the genes encoding the RNA polymerase alpha subunit (rpoA), phenylalanyl-tRNA synthase alpha subunit (pheS), translational elongation factor Tu (tuf), and Hsp60 chaperonins (groEL) also allowed to classify the analyzed isolate as Lb. helveticus. Further classification at the strain level was achieved by sequencing of the slp gene. This gene showed 99.8% identity with the corresponding slp gene of Lb. helveticus R0052, which is in good agreement with data obtained by nano-HPLC coupled to an LTQ-FT-ICR mass spectrometer. Finally, LC-MS/MS analysis of surface layer proteins extracted from three other Lactobacillus strains proved that the proposed method is the appropriate molecular tool for the identification of S-layer-possessing lactobacilli at the species and even strain levels.

Consumer Preferred Formats of Nutrition Labels - Housewives of Daejon City - (소비자가 선호하는 영양표시 형태 - 대전 지역 주부를 중심으로 -)

  • 최지현;정영지
    • Korean Journal of Community Nutrition
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    • v.8 no.2
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    • pp.220-230
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    • 2003
  • The purpose of this study was to investigate consumer preferred formats of nutrition labels: the type of nutritional information provided on the labels. A survey was conducted on 222 housewives between the age of 20 and 65 in Daejeon city in 1998, using an interview questionnaire showing illustrated nutritional label formats. Among twelve nutrients and caloric values listed on the nutrition labels, the housewives ranked their interest in the items in the following order of calorie (58.56%), calcium (56.76%), cholesterol (54.05%), protein (39.19%) and vitamin C (36.04%). The majority of the subjects (69.37%) checked labels when they bought milk or dairy products, whereas only 8.11% looked at labels when they bought Ramyon or noodles. Most of the subjects (90.99%) preferred products with labels with nutritional information as compared with products without labels. this was especially so true of the younger individuals and of pc-ople who checked food labels more frequently. The subjects preferred labeling which described the nutrient content per Package (55.41%), sewing size (32.88%) and food weight of 1009 (11.71%) as a standard nit. Generally, they prefer-red a simplified list to a detailed list of the number of nutrients, a figure graph to bar graph in nutrition claim, vertical bar graph to horizontal bar graph showing the nutritional content of foods and a web-type graphic format to a radiation-format when describing the item's percentage of the Korean RDA's nutrient content. The subjects who checked labels frequently especially preferred detailed list, while housewives with job or with unhealthy family members preferred a simplified list and graphic, rather than a numerical display. From this result, it is suggested that providing consumers with more nutrients information in a more easily understood graphic format would encourage the consumers to check nutritional labels. Therefore this should be considered when developing the regulations far nutrition labeling or when educating the Public about nutrition. (Korean J Community Nutrition 8(2) : 220∼230, 2003)

Comparative Effect of Spectacles and Orthokeratology Lenses on Axial Elongation in Children with Mild-to-Moderate Myopia (중등도 이하 소아 근시에서 안경과 각막굴절교정렌즈 착용이 안축장 성장에 미치는 영향 비교)

  • Kim, Tae Hee;Kim, Myung Hun;Kwag, Joo Young;Choi, Jin Seok;Pak, Kyu Hong;Lee, Kyeongwook;Park, Taeseong;Chung, Sung Kun;Hyun, Joo
    • Journal of The Korean Ophthalmological Society
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    • v.59 no.11
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    • pp.1009-1016
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    • 2018
  • Purpose: To assess the effect on axial elongation and associated factors between spectacles and of orthokeratology lens (OK) wearing in children with mild to moderate myopia. Methods: A total of one hundred subjects, ranging in age from 6 to 13 years, and with mild to moderate myopia no more than -4.50 diopters in spherical equivalent, visited our clinic from 2013 to 2015. The OK group (75 eyes) and the spectacles group (64 eyes) were compared and analyzed on the axial elongation and associated factors. Results: In the OK group, axial length was elongated in 1 year period with a mean increase of $0.24{\pm}0.29mm$. In spectacles group, axial length was elongated in 1 year period with a mean increase of $0.42{\pm}0.20mm$. The statistically significant suppression of axial elongation was observed in OK group compared to the spectacles group (Mann-Whitney U test, p < 0.05). For OK group, the age of starting OK (Pearson's correlation, r = -0.481, p < 0.05) was the only influencing factor on axial elongation, which had negative correlation with axial elongation. In spectacles group, the age of starting spectacles had negative correlation with axial elongation (Pearson's correlation, r = -0.462, p < 0.05) and baseline spherical equivalent, spherical diopter, cylindrical diopter from manifest refraction had positive correlation with axial elongation. Comparison of axial elongation in orthokeratology lens group and spectacles group by age groups (6 to 9 years [28 eyes], 9 to 13 years [47 eyes]), 9 to 13 years of orthokeratology lens group had the stronger suppression of axial elongation (Mann-Whitney U test, p < 0.05). Conclusions: The OK effectively suppresses axial elongation compared to the spectacles. Although the patients are in age from 9 to 13 years, the axial elongation was effectively suppressed.

The Treatment Characteristics of Intermittent Aeration and Conventional Activated Sludge Processes According to the Changes of Temperature and pH (온도 및 pH 변화에 따른 연속 및 간헐 포기식 활성슬러지법의 처리 특성)

  • Lee, Jeoung-Su;Lee, Tae-Kyoo
    • Journal of Korean Society of Environmental Engineers
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    • v.22 no.6
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    • pp.1001-1009
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    • 2000
  • This study is to find the utilization of intermittent aeration system, around S-COD, T-COD, SS, timewise changes of treatment performance, sludge conversion yield, changes of temperature and pH, etc. In consequence of this study, factors of temperature correction showed 1.052 on continuous aeration, and 1.056 on intermittent aeration which is more sensitive to temperature through a minute degree. Meanwhile, sludge conversion yield on intermittent aeration showed lower and more economical than that on continuous aeration. In case of changing pH, treatment water of both reactors worsened slightly in acid but improved in alkali. In general. considering the quality of effluent water, variation pH of effluent water, etc. the case of intermittent aeration was more favorable than that of continuous aeration.

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