• Journal of KIISE:Software and Applications
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• v.36 no.12
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• pp.1009-1015
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• 2009

Handwriting verification is a technique of distinguishing the same person's handwriting specimen from imitations with any two or more texts using one's handwriting individuality. This paper suggests an effective verification method for the handwritten signature or text on the off-line environment using pattern recognition technology. The core processes of the method which has been researched in this paper are extraction of letter area, extraction of features employing structural characteristics of handwritten text, feature analysis employing DTW(Dynamic Time Warping) algorithm and PCA(Principal Component Analysis). The experimental results show a superior performance of the suggested method.

• The Korean Journal of Oral and Maxillofacial Pathology
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• v.41 no.1
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• pp.9-16
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• 2017

Porphyromonas gingivalis is a gram-negative bacteria of rod shape, and grown in an anerobic condition. It colonizes in subgingival crevice and is known as a major pathogen causing chronic periodontitis. It possesses an invasive property and replicative potential within various cell types, presumably playing an important role in modulating biological behaviors of oral cancer. However, the pathophysiology of P. gingivalis in the malignant transformation of oral cancer has not been fully understood. In this study, we aimed to investigate molecular changes of oral squamous cell carcinoma cells induced by repetitive P. gingivalis infection that clinically resembles chronic periodontitis.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.5
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• pp.1009-1019
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• 2009

In this research, various characteristics of South Korea's design flood have been examined by deriving appropriate design flood, using data obtained from careful observation of actual floods occurring in selected main watersheds of the nation. 19 watersheds were selected for research in Korea. The various characteristics of annual rainfall were analyzed by using a moving average method. The frequency analysis was decided to be performed on the annual maximum flood of succeeding one year as a reference year. For the 19 watersheds, tests of basic statistics, independent, homogeneity, and outlier were calculated per period of annual maximum flood series. By performing a test using the LH-moment ratio diagram and the Kolmogorov-Smirnov (K-S) test, among applied distributions of Gumbel (GUM), Generalized Extreme Value (GEV), Generalized Logistic (GLO) and Generalized Pareto (GPA) distribution was found to be adequate compared with other probability distributions. Parameters of GEV distribution were estimated by L, L1, L2, L3 and L4-moment method based on the change in the order of probability weighted moments. Design floods per watershed and the periods of annual maximum flood series were derived by GEV distribution. According to the result of the analysis performed by using variation rate used in this research, it has been concluded that the time for changing the design conditions to ensure the proper hydraulic structure that considers recent climate changes of the nation brought about by global warming should be around the year 2002.

• Fashion & Textile Research Journal
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• v.15 no.6
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• pp.1000-1009
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• 2013

Well-fitting riding breeches provide a comfortable ride. Horse-riding breeches should fit the lower body with patches located on the inside of hip and knees to prevent tears and slips. This study provides information about the fit of women's breeches using 3D human body scan data wearing commercially available two breeches according to posture. To get information about breeches fit, we measured the angle of waist line, the length, and the area of the breeches fit on four 20's women. This research showed the problem which was down for waist back line in the case of the horseback riding pose. The back waistline of the riding breeches should be raised compared to the front waistline; subsequently, an increased waistline angle results in less back waistline decrease. The breeches have plenty of length from back waist to crotch so the breech fit can be improved. The thigh circumference increased when riding in the front; therefore, good elasticity of the weft direction of the fabric is recommended. The length increase and the peripheral increase of the front knee significantly increased the surface area of the knee; consequently, knee patch material should be a two-way elastic fabric in all directions to enhance comfort according to riding motion.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.58 no.5
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• pp.1004-1009
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• 2009

Recently, mass spectrometers are widely used for in-situ chemical analysis. It has rapid response and high sensitivity. In this paper, we present the fabrication and test of a cold cathode emitter for micro mass spectrometer using CNTs(Carbon nano tubes). The CNTs have good mechanical, electrical and chemical characteristics. So they have a long life time and strong robustness. The micro mass spectrometer is composed of the glass substrate and the silicon substrate. The glass substrate is constructed by electrodes for TOF(Time-of-flight) which analyze an ion with mass to charge ratio as ion separator. The silicon substrate is highly doped wafer which is patterned for gate electrode and then 100 11m dry etching to grow the CNTs as the electron emitter. The CNTs are grown by HFCVD(Hot filament chemical vapor deposition) with sputtering the catalyst. We successfully attained to grow the CNTs and to test the characteristics.

• ALGAE
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• v.38 no.2
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• pp.111-126
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• 2023

Mixotrophic dinoflagellates act as primary producers, prey, and predators in marine planktonic food webs, whereas exclusively autotrophic dinoflagellates are primary producers and prey. Species of the dinoflagellate genus Scrippsiella are commonly found in marine ecosystems and sometimes cause harmful red tides. Among the 28 formally described Scrippsiella species, S. acuminata has been found to be mixotrophic and two unidentified species have been found to be mixotrophic. To determine whether the other species in this genus are similarly mixotrophic, the mixotrophic ability of S. donghaiensis SDGJ1703, S. lachrymosa SLBS1703, S. masanensis SSMS0908, S. plana SSSH1009A, and S. ramonii VGO1053 was explored using 15 potential prey items, including 2-㎛ fluorescently labeled microspheres (FLM) and heterotrophic bacteria (FLB), the cyanobacterium Synechococcus sp., and various microalgal prey species. The ability of S. acuminata to feed on FLM and FLB was also investigated. We found that S. donghaiensis, S. lachrymosa, S. masanensis, S. plana, and S. ramonii did not feed on any potential prey tested in this study, indicating a lack of mixotrophy. However, S. acuminata fed on both FLM and FLB, confirming its mixotrophic ability. These results lowered the proportion of mixotrophic species relative to the total number of tested Scrippsiella species for mixotrophy from 100% to 29-38%. Owing to its mixotrophic ability, S. acuminata occupies an ecological niche that is distinct from that of S. donghaiensis, S. lachrymosa, S. masanensis, S. plana, and S. ramonii.

• Journal of Microbiology and Biotechnology
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• v.21 no.2
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• pp.147-153
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• 2011

Bacillus species have been involved in metal association as biosorbents, but there is not a clear understanding of this chelating property. In order to evaluate this metal chelating capacity, cultures and spores from Grampositive bacteria of species either able or unable to produce surface layer proteins (S-layers) were analyzed for their capacity of copper biosorption. Only those endowed of S-layers, like Bacillus sphaericus and B. thuringiensis, showed a significant biosorption capacity. This capacity (nearly 50%) was retained after heating of cultures, thus supporting that structural elements of the envelopes are responsible for such activity. Purified S-layers from two Bacillus sphaericus strains had the ability to biosorb copper. Copper biosorption parameters were determined for strain B. sphaericus 2362, and after analyses by means of the Langmuir model, the affinity and capacity were shown to be comparable to other bacterial biosorbents. A competitive effect of $Ca^{2+}$ and $Zn^{2+}$, but not of $Cd^{2+}$, was also observed, thus indicating that other cations may be biosorbed by this protein. Spores that have been shown to be proficient for copper biosorption were further analyzed for the presence of S-layer content. The retention of S-layers by these spores was clearly observed, and after extensive treatment to eliminate the S-layers, the biosorption capacity of these spores was significantly reduced. For the first time, a direct correlation between S-layer protein content and metal biosorption capacity is shown. This capacity is linked to the retention of S-layer proteins attached to Bacillus spores and cells.

• Journal of Microbiology and Biotechnology
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• v.21 no.3
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• pp.243-251
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• 2011

The gene bolA was discovered in the 80's, but unraveling its function in the cell has proven to be a complex task. The BolA protein has pleiotropic effects over cell physiology, altering growth and morphology, inducing biofilm formation, and regulating the balance of several membrane proteins. Recently, BolA was shown to be a transcription factor by repressing the expression of the mreB gene. The present report shows that BolA is a transcriptional regulator of the dacA and dacC genes, thus regulating both DD-carboxypeptidases PBP5 and PBP6 and thereby demonstrating the versatility of BolA as a cellular regulator. In this work, we also demonstrate that reduction of cell growth and survival can be connected to the overexpression of the bolA gene in different E. coli backgrounds, particularly in the exponential growth phase. The most interesting finding is that overproduction of BolA affects bacterial growth differently depending on whether the cells were inoculated directly from a plate culture or from an overnight batch culture. This strengthens the idea that BolA can be engaged in the coordination of genes that adapt the cell physiology in order to enhance cell adaptation and survival under stress conditions.

• The Research Journal of the Costume Culture
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• v.17 no.6
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• pp.1009-1020
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• 2009

This study aims to express the androgynous image via shirts as a fashion item. Shirts are widely worn as a fashion item regardless of sex and age, with growing importance as a casual outer, with the increase in leisure activities driven by the recent implementation of the 5-Day Work Week system in Korea. As for the theoretical background, the study was reviewed previous studies of books, thesis, a series of publication, and the Internet sites on this topic. Through a careful analysis of these previous studies, it designed and made shirts that inspired by androgynous image. Conclusions of this study are as follows: First, the study found that meanings of symbolism in clothing continue to change, not fixed at all, depending on historic and cultural environments, and so does symbolism for femininity and masculinity of clothing. Second, shirts are widely worn as a fashion item regardless of sex and age, with growing importance as a casual outer, with the increase in leisure activities driven by the recent implementation of the 5-Day Work Week system in Korea. Third, two patters were used for the work in this study in order to emphasize its form, along with mono color white and stripe patterns. For materials, cotton and blend as a most basic material for a shirt were used with unique variations in the form. Fourth, decorative details or trimming such as ribbon tying methods, shirring, attaching in layers, and irregular pleading widely used for women's wear were applied, and silhouettes with strong drape feelings were used to add feminine feature to men' shirts, in an effort to propose a fashion design of the androgynous look. Fifth, clothes proposed in this study are different from feminine clothing item blouse, because they are androgynous shirts mixing masculinity and femininity. Stiff pads were used in collars and cuffs characteristics of men's traditional shirts to maintain masculinity of a shirt, and design was developed by adding feminine decorative elements, which is different from women's blouse.

• Journal of Microbiology and Biotechnology
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• v.21 no.3
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• pp.284-292
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• 2011

An endocellulase-free multienzyme complex was produced by a thermophilic anaerobic bacterium, Thermoanaerobacterium thermosaccharolyticum strain NOI-1, when grown on xylan. The temperature and pH optima for growth were $60^{\circ}C$ and 6.0, respectively. The bacterial cells were found to adhere to insoluble xylan and Avicel. A scanning electron microscopy analysis showed the adhesion of xylan to the cells. An endocellulase-free multienzyme complex was isolated from the crude enzyme of strain NOI-1 by affinity purification on cellulose and Sephacryl S-300 gel filtration. The molecular mass of the multienzyme complex was estimated to be about 1,200 kDa. The multienzyme complex showed one protein on native PAGE, one xylanase on a native zymogram, 21 proteins on SDS-PAGE, and 5 xylanases on a SDS zymogram. The multienzyme complex consisted of xylanase, ${\beta}$-xylosidase, ${\alpha}$-L-arabinofuranosidase, ${\beta}$-glucosidase, and cellobiohydrolase. The multienzyme complex was effective in hydrolyzing xylan and corn hulls. This is the first report of an endocellulase-free multienzyme complex produced by a thermophilic anaerobic bacterium, T. thermosaccharolyticum strain NOI-1.