• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.380-387
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• 2015

Aronia (Aronia melanocarpa, Black chokeberry) is an important cash crop in domestic agriculture. We investigated the effects of plant growth regulators on shoot proliferation and rooting using in vitro tissue culture. The most effective shoot multiplication was observed on WPM (woody plant medium) supplemented with 1.0 mg/L zeatin ($8.3{\pm}1.0$ shoots/explant), while the highest rooting rate was obtained from half-strength WPM with 3.0 mg/L IBA (8.8 roots/explant). The rooted plantlets all survived in the artificial soil mixture (with a mixture of peat moss : perlite : vermiculite, 1:1:1, v/v/v) and grew up relatively uniform, ranging from 14 to 16 leaves, 8 to 10 cm in stem height, and 2.3 to 2.8 mm in stem diameter. While experimenting with 5 different varieties of Aronia, we found out that each variety had different characteristics of shoot proliferation and rooting. The total numbers of proliferated shoots per variety is as follows: $17.4{\pm}0.8$ for Nero, 14 to 15 for Purple and Mackenzie, and 10 for both Viking and Odamamachiko. Rooting rates were also various depending on the variety: 88% of Odamamachiko, 80% of Viking and Purple, and 76% of Nero and 60% of Mackenzie shoots rooted. The survival rate of the rooted plantlets was from 92% to 100%, varying by type. Further growth appeared to be better in auxin-treated plantlets, compared to untreated ones. Our results showed the possibility of establishing an effective in vitro micropropagation system for Aronia melanocarpa.

• Proceedings of the Korean Society of Crop Science Conference
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• 2000.04a
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• pp.262-263
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• 2000

• Journal of Korean Society of Forest Science
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• v.66 no.1
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• pp.74-78
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• 1984

The highest cutting ratio and the best growth performance of the P. alba, P. alba ${\times}$ P. glandulosa and P. glandulosa were at -0.05 bars of soil water potential (${\Psi}_s$). Their growth and rooting ability of cuttings were better in suitable soil moisture contents, 19.650, but were worse in low soil moisture contents, 11.41% and 16.66%, respectively. Specially, P. alba ${\times}$ P. glandulosa appeared hybrid vigor in high soil water potential (${\Psi}_s$= -0.05 bars) but not appeared it below ${\Psi}_s$ = -0.5 bars. It is believed that P. glandulosa is a natural hybrid between P. davidiana and P. alba in view of morphological characteristics. Aspen, normally, does not have the rooting ability of cuttings, however, P. glandulosa showed the rooting ability, though the ratio was low, 23%. It is estimated that this rooting ability was originated from P. alba. In addition, its growth rate was between P. alba and P. davidiana, and the sensitivity of water requirement for the growth was also really equal to P. alba. This facts prove P. glandulosa to be natural hybrid between P. alba and P. davidiana.

• Korean Journal of Plant Resources
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• v.23 no.4
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• pp.368-373
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• 2010

Juniperus chinensis var. sargentii Henry is a short and creeping evergreen shrub which reaches about 60 cm in height and only occurs in the northeast Asia and in the top of high mountains over the Korea. The Korea Forest Service protects it strictly by law since J. chinensis var. sargentii is an eccentric plant and possibly may be exterminated soon in Korea. This study was carried out to develop the propagation technique by cutting for conservation of genetic resources of J. chinensis var. sargentii. The rooting responses of branch cuttings, obtained from hard(May) and semi-hard wood shoots (August) to four growth regulators, namely, IAA, IBA, NAA and Rooton(exceptionally powder method) applied at various concentrations(0, 100, 200, 500, 1000 and 2000 $mgL^{-1}$) were examined in mixed soil media. Rooting rate showed significant difference between cutting times, among kinds and among concentrations of growth regulators. The optimum cutting time was April to May in hardwood cutting. The application of IBA 1000 $mgL^{-1}$(rooting rate : 36.4%) was most effective in callus formation and rooting of cutting. Relatively, rooting of cutting of the control taken in May was 30.4%. Root characteristics such as number, length and diameter of root were not significantly affected by kinds and concentrations of growth regulators in hardwood cutting.

• Horticultural Science & Technology
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• v.30 no.2
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• pp.129-136
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• 2012

This study was conducted to develop an efficient mass propagation method for the mature $Prunus$ $yedoensis$ Matsumura (43 to 58 years old). Cutting was conducted depending on cutting time, auxin treatments (IBA and NAA treatments mixed with talc powder), and cuttings position on shoots in a plastic house equipped with a fog system without heating. Rooted cuttings were transplanted to a nursery bed, and their growth characteristics were investigated in order to check whether the cuttings are successful or not for roadside tree planting. The average rooting rate was highly significant ($P$ < 0.0001) in all treatments: cutting on June 1st (61.4%) was more than two times greater in rooting rate than that on August 1st (23.6%); IBA 1,000 $mg{\cdot}L^{-1}$ (90.8%) and IBA 500 $mg{\cdot}L^{-1}$ (89.2%) showed much greater rooting rates than those of the other treatments; upper part of the cuttings treated with IBA 1,000 $mg{\cdot}L^{-1}$ showed the highest rooting rate, 96.7%. The interactions among treatments in the average rooting rate were also significant. There were significant differences ($P$ < 0.0001) among the auxin treatments in the survival rate of leafed cuttings transplanted to a nursery bed. The average survival rate was 46.5%, and IBA 1,000 $mg{\cdot}L^{-1}$ treatment was the highest in leafed cuttings 79.2%, but most of leafless cuttings were dead. There were significant differences ($P$ < 0.0001) among the cuttings, grafts, and in the seedlings height, diameter at root collar, the number of roots, branches, and leaves, etc., and the cuttings was the best. We can expect a possibility of mass propagation of improved $P.$ $yedoensis$ Matsumura and a high planting survival rate through the transplanting of cuttings to a nursery bed in which the cuttings should be the following conditions: cutting in June to July, use of the upper part of cuttings, IBA treatment, and rooting in August in a cutting-greenhouse equipped with a fog system.

• Proceedings of the Korean Society of Plant Biotechnology Conference
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• 2004.10a
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• pp.177-177
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• 2004

• Journal of Bio-Environment Control
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• v.20 no.4
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• pp.346-351
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• 2011

This research was done to identify the mass propagation method in winter daphne (Daphne odora Thunb) using its softwood cuttings and to investigate its plant characters established at different light and soil conditions as an indoor plant. Cuttings from winter daphne were taken and grown in different treatment consisted of rooting media (perlite, vermiculite, perlite + vermiculite (1 : 1) and commercial horticulture media soil), indole butyric acid (IBA) hormone concentrations (0, 100, 500, 1000 ppm and Rootone) and date of cutting. Transplants were grown at different light intensities (100, 1000, 2500 lux and control) and growing media. Results showed that cuttings grown in perlite + vermiculite (1 : 1) gave higher percentage (100%) rooting. Cuttings treated with Rootone and IBA 100 ppm showed good rooting growth and cutting taken in June, 25 gave the highest rooting (96.7%). The best plant growth obtained at 1000 lux (56~60 ${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) followed by 2500 lux (125~130 ${\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$) and scoria mixed with commercial horticulture media soil showed better growth of transplants.

• Korean Journal of Breeding Science
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• v.41 no.4
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• pp.482-487
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• 2009

The apple rootstock M.26 (Malus pumila) is very popular apple rootstock with semi-dwarf habit and the trees on M.26 produce high quality fruit at a young age. Since it is prone to poor prop ability to soil, however, young trees require staking in windy locations. The rolC gene was introduced into M.26 by Agrobacterium tumefaciens LBA4404 harboring pBI121 to obtain its transformants with dwarfism and enhanced rooting ability. One regenerated transgenic line was confirmed by polymerase chain reaction (PCR) analysis and Southern blot analysis of genomic DNA for the existence of rolC gene. The characteristics of transgenic line in vitro were not significantly different from non-transgenic line except for the active root formation and lateral root number. The rolC transgenic line showed reduced stem length and increased root number in vitro. Rooting ability was examined in the isolated greenhouse after mound layering. Compared to non-transgenic M.26, rolC transgenic line showed significantly higher rooting ability. The transgenic line did not show any other observable variation in shoot phenotype compared with non-transgenic line excepting increased branching

• Asian Journal of Turfgrass Science
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• v.17 no.4
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• pp.129-146
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• 2003

Research was initiated to compare establishment vigor, uniformity, rooting potential and turf quality in sods of cool-season grasses (CSG). Several turfgrasses grown under pure sand soil were tested. Establishment vigor, uniformity, rooting potential and turf quality were evaluated in the study. Turfgrass entries were comprised of three blends from Kentucky bluegrass (KB, Poa pratensis L.), perennial ryegrass (PR, Lolium perenne L.), and tall fescue (TF, Festuca arundinacea Schreb.), respectively and three mixtures among them. Differences by treatments were significantly observed in establishment vigor, uniformity, rooting potential and turf quality. Early establishment vigor was mainly influenced by germination speed, being fastest with PR, intermediate with TF and slowest with KB. In a late stage of growth, however, it was affected more by growth habit, resulting in highest with KB and slowest with TF. There were considerable variations in sod uniformity among turfgrasses. Best uniformity among monostand sods was associated with KB, while poorest one with TF. PR sod produced intermediate uniformity between KB and TF. The uniformity of polystand sods of CSG mixtures was inferior to that of monostands of KB, PR and TF, due to characteristics of mixtures comprised of a variety of color, density, texture and growth habit. The greatest potential of sod rooting was found with PR and the poorest with KB. Intermediate potential between PR and KB was associated with TF. In CSG mixtures, it was variable, depending on turfgrass mixing rates. Generally, the higher the PR in mixtures, the greater the sod rooting potential. At the time of sod harvest, however, turfgrass quality of KB was superior to that of PR. because of its characteristics of uniform surface, high density and good mowing quality. These results suggest that a careful expertise based on turf quality as well as sod characteristics like establishment vigor, uniformity and rooting potential be strongly required for the success of golf course or athletic field in establishment.

• Korean Journal of Organic Agriculture
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• v.8 no.1
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• pp.89-99
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• 1999

To do mass multiplication of plug seedlings in potatoes, apical stem cuttings originated from virus-free microtubers were cut to one-two internodes and transplanted into the plug-tray. After 10days, we applied Chitosan and Wood Extraction on rooting and tuber formation of plug seedlings. To improve field adaptability of plug seedlings, rooted cuttings with a height of 20cm after 20days of cutting were transplanted ito the fields, We applied 500~2000ppm Chitosan on growth characteristics and tuber formation of that. The above and underground growths, such as plant height and number of leaves were significantly more vigorous after treatment with 500~1,000ppm Chitosan and 2,000ppm Wood Extraction, the spray treatment was carried out five times at intervals of four days after ten days of transplanting. T-N, K, P, Mg and Na, were higher as the concentrations of chitosan and Wood Extraction were higher. The growth and tuber yield in plug seedlings planting plot and seed potatoes planting plot were effectively highter as foliar application of Chitosan(500~2,000ppm) was done after planting the plot. T-N content in leaves and tuber was higher as the concentration of Chitosan was high. A similar tendency was shown in K, P and Mg. In the small tuber(under 30g), the number of tubers and tuber yield were relatively increased in the seed potatoes planting plot, but the large tubers(over 80g) yield was higher in the plug seedlings planting plot, and in order to increase tuber yield in plug seedlings it was necessary to add plant density to the field.