• Title/Summary/Keyword: Rice seeds

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Studies on black rot of rice seedlings (벼 검은빛 모썩음병에 관한 연구)

  • Cho Y. S.
    • Korean journal of applied entomology
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    • v.4
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    • pp.25-28
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    • 1965
  • The study was carried on a new disease of rice seedling which named 'Black rot of rice seedling'. None of papers on this disease was reported recently. The symptom of this disease was quite different from other seedling rot of rice plant. Submerged mycelial growth in water was black colored and it was surrounded whole surface of seed. Soil on which diseased seedlings were placed was black colored by this disease and the seedlings, finally stopped to grow within 3 em in height, and appeared with black rot symptom. Sporulation of the pathogen does not occurred under both of natural and artificial conditions. The mycelium appeared in dark color and with distinguished septation under the microscope. With those results it is easily recognized that the pathogen does not belong to those which causal organism:; of other seedling rot of rice plant like as Phycomycetes or Fusarium species. The study should be continued on identification of the causal organism. Favorable environments for the disease did not differ from other seedling rot of rice plant, and varietal resistance also did not significantly differ among the varieties used in this experiment. Dissemination of the disease was carried out not by planted seeds but by infected soil.

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Evaluation for anaerobic germinability of rice germplasm for direct-seeding cultivation under submerged conditions

  • Rauf, Muhammad;Choi, Yu-Mi;Lee, Sukyeung;Lee, Myung-Chul;Oh, Sejong;Hyun, Do Yoon
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.71-71
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    • 2017
  • Stable stand establishment is pre-requisite in direct rice seeding system for obtaining optimal yield of rice crop in rain-fed and waterlogged areas. Anaerobic condition on waterlogged soil causes low germination which significantly reduces crop yield. Due to low availability of tolerant genetic material for anaerobic germination, there is urgent need to evaluate rice germplasm for better germinability under anaerobic conditions. Seeds of the 185 rice accessions were evaluated for germination vigor and coleoptile length under anaerobic conditions. The variation among germplasm was tested for significance using analysis of variance and various multivariate components. Significant level of variation was observed among all accessions for germination vigor and coleoptiles length. Although highest mean values for coleoptiles length (2.1cm) and germination rate (60%) were observed in japonica accessions but maximum coleoptile length (4.68cm) and germination rate (96%) was found in indica genotype CO18. A highly significant and positive correlation was also observed between germination vigor and coleoptiles length, which signify the importance of elongated coleoptile under anaerobic conditions. The PCA analysis illustrated that 97.24% variation was accounted by PC1 while PC2 and PC3 explained 2.54% and 0.24% variation for germination vigor and coleoptile length. PCA scattered plot divided the accessions in four various groups. All AG tolerant accessions were included in group I. Likewise in the case of cluster analysis, two major clades (I and II) were formed. All accessions showing >40% germination rate were included in clade I, whereas all other accessions with <40% germination rate were grouped in clade II. Further more highly tolerant accessions (>80% germination) were grouped in sub-cluster IA.

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Variation of Tocopherol Composition and Morphology in Soybean [Glycine max (L.) Merr.] Germplasms

  • Kim, Young-Jin;Oh, Young-Jin;Cho, Sang-Kyun;Lee, Mi-Ja;Lee, Kwang-Won;Kim, Kee-Jong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.57 no.1
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    • pp.83-88
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    • 2012
  • In order to get information on tocopherol content and composition in fifty-six soybean germplasms were evaluated by HPLC. From the principle component analysis, the first three components accounted for 71.6% of the total variance of tocopherol content in the germplasms. Principal component 1 showed significant correlations with all the morphological markers except 100-seed weight. Soybean germplasms were divided into three groups by the first two principal components. The highest content of ${\alpha}$-tocopherol was $38.6{\mu}g$ per g in Tanner, while that of ${\gamma}$-tocopherol was $195.6{\mu}g$ in PI 91073. In case of ${\delta}$-tocopherol, IT 105622 showed the highest value as $29.8{\mu}g$. The contents of tocopherol were gradually increased from the late August to late September, which was 10 days before maturity, in Alchankong and PI 96322. The tocopherol content was higher in seeds from the plants sown early than those sown late. Total tocopherol contents of PI 96188, Geomjeongkong 2, and Suwon 183 grown in Gimje were higher than those grown in Iksan, but the difference was not observed in PI 96322. These results suggest that the contents of tocopherol in soybean were affected not only by the genotypes but also by environment.

A TILLING Rice Population Induced by Gamma-ray Irradiation and its Genetic Diversity

  • Cho, Hyun Yong;Park, Seo Jung;Kim, Dong Sub;Jang, Cheol Seong
    • Korean Journal of Breeding Science
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    • v.42 no.4
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    • pp.365-373
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    • 2010
  • TILLING (Targeting Induced Local Lesions IN Genomes) is broadly regarded as an excellent methodology for reverse genetics applications. Approximately 15,000 $M_3$ TILLING lines have been developed via the application of gamma-ray irradiation to rice seeds (cv. Donganbyeo), followed by subsequent selections. In an effort to evaluate the genetic diversity of the TILLING population, we have employed the AFLP multiple dominant marker technique. A total of 96 (0.64%) TILLING lines as well as Donganbyeo were selected randomly and their genetic diversity was assessed based on AFLP marker polymorphisms using 5 primer combinations. An average of 100.4 loci in a range of 97 to 106 was detected using these primer combinations, yielding a total of 158 (31.4%) polymorphic loci between Donganbyeo and each of the 96 lines. A broad range of similarity from 80% to 96% with an average of 89.4% between Donganbyeo and each of the 96 lines was also observed, reflecting the genetic diversity of the TILLING population. Approximately 28 polymorphic loci have been cloned and their sequences were BLAST-searched against rice whole genome sequences, resulting in 20 matches to each of the gene bodies including exon, intron, 1 kb upstream and 1 kb downstream regions. Six polymorphic loci evidenced changes in the coding regions of genes as compared to the rice pseudomolecules, 4 loci of which exhibited missense mutations and 2 loci of which exhibited silent mutations. Therefore, the results of our study show that the TILLING rice population should prove to be a useful genetic material pool for functional genomics as well as mutation breeding applications.

Pre-harvest Sprouting Tolerance Test in Rice with Floury Endosperm

  • Su Kyung Ha;Seo Ho Shin;Hyun-Sook Lee;Chang-Min Lee;Seung Young Lee;Jae-Ryoung Park;Ji-Ung Jeung
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.213-213
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    • 2022
  • Pre-harvest sprouting(PHS) refers to germinating seeds in the mother plant before harvesting under low dormancy and humid climate, deteriorating grain quality, and rice yield. Rice varieties with floury endosperm(RFE) have been developed to boost domestic rice consumption by invigorating the processed rice industry, reducing milling and environmental cost. However, the PHS tolerance of RFE is relatively low in the rice varieties with transparent endosperm(RTE) since they soak moisture rapidly due to soft endosperm. In this study, Baromi2(BR2), floury endosperm, and Jomyeong1(JM1), PHS tolerance donor, were crossed to improve PHS tolerance. Major agronomic traits and PHS tolerance test of ten F7(BR2/JM1) lines were conducted in NICS, 2022. The evaluations of PHS were carried out according to the method of RDA(2012) with slight modifications. Briefly, three panicles were treated and incubated 25℃ in a growth chamber 35 days after the heading date. Ten PHS tolerance promising lines demonstrated floury endosperm. The heading date of BR2 and JM1 was 7/27 and 8/5, respectively. The heading date of promising lines was 7/23~8/10. The PHS rate of BR2 and JM1 exhibited 56.3% and 10.7%, respectively. However, the PHS rate often promising lines demonstrated 2.4%~52.4%, 3 lines significantly lower than BR2. Further studies such as ABA contents are necessary to elucidate the mechanism of PHS tolerance in BR2/JM1. These results may contribute to developing elite RFE lines with improved PHS tolerance.

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Production of Exopolysaccharides and İndole Acetic Acid (IAA) by Rhizobacteria and Their Potential against Drought Stress in Upland Rice

  • Tetty Marta Linda;Jusinta Aliska;Nita Feronika;Ineiga Melisa;Erwina Juliantari
    • Journal of Microbiology and Biotechnology
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    • v.34 no.6
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    • pp.1239-1248
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    • 2024
  • Peatlands are marginal agricultural lands due to highly acidic soil conditions and poor drainage systems. Drought stress is a big problem in peatlands as it can affect plants through poor root development, so technological innovations are needed to increase the productivity and sustainability of upland rice on peatlands. Rhizobacteria can overcome the effects of drought stress by altering root morphology, regulating stress-responsive genes, and producing exopolysaccharides and indole acetic acid (IAA). This study aimed to determine the ability of rhizobacteria in upland rice to produce exopolysaccharides and IAA, identify potential isolates using molecular markers, and prove the effect of rhizobacteria on viability and vigor index in upland rice. Rhizobacterial isolates were grown on yeast extract mannitol broth (YEMB) medium for exopolysaccharides production testing and Nutrient Broth (NB)+L-tryptophan medium for IAA production testing. The selected isolates identify using sequence 16S rRNA. The variables observed in testing the effect of rhizobacteria were germination ability, vigour index, and growth uniformity. EPS-1 isolate is the best production of exopolysaccharides (41.6 mg/ml) and IAA (60.83 ppm). The isolate EPS-1 was identified as Klebsiella variicola using 16S rRNA sequencing and phylogenetic analysis. The isolate EPS-1 can increase the viability and vigor of upland rice seeds. K. variicola is more adaptive and has several functional properties that can be developed as a potential bioagent or biofertilizer to improve soil nutrition, moisture and enhance plant growth. The use of rhizobacteria can reduce dependence on the use of synthetic materials with sustainable agriculture.

Investigation of Main Dishes on Literatures before the 17th Century (17세기 이전 주식류의 문헌적 고찰)

  • Kim, Eun-Mi;Cho, Shin-Ho;Chung, Rak-Won;Choi, Young-Jin;Won, Sun-Im;Cha, Gyung-Hee;Kim, Hyun-Sook;Lee, Hyo-Gee
    • Korean journal of food and cookery science
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    • v.22 no.3 s.93
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    • pp.314-336
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    • 2006
  • In this treatise, we investigated the Korean literature of main dishes before the 17th century : ${\ulcorner}Sangayorock{\lrcorner}$, ${\ulcorner}Sasichanyocho{\lrcorner}$, ${\ulcorner}Soowonjabbang{\lrcorner}$, ${\ulcorner}Yongjechongwha{\lrcorner}$, ${\ulcorner}Dongyoebogam{\lrcorner}$, ${\ulcorner}Domoondaejak{\lrcorner}$, ${\ulcorner}Geebongyouseul{\lrcorner}$, ${\ulcorner}New-Guwhangchalyo{\lrcorner}$, ${\ulcorner}Eumshickdimibang{\lrcorner}$,, ${\ulcorner}joobangmoon{\lrcorner}$, and ${\ulcorner}Yorock{\lrcorner}$. Main dishes were classified 19 kinds of rice, 64 kinds of gruel, 6 kinds of thin gruel, 1 kind of Moori, 1 kind of rice-cake soup, 37 kinds of noodles and 20 kinds of dumpling. Rice varieties were brown rice, rice, hulled rice, millet rice, foxtail millet rice, barley rice, sesame rice and flax seed rice. Gruel ingredients were cereal, beans, vegetables, nuts or seeds, medical plants or hardy wild plants, poultry, pork's kidney, dog's liver or oyster, clam and/or milk Rice-cake soup was 'Tangbyun' in ${\ulcorner}Domoondaejak{\lrcorner}$. Noodles were divided by main flours: wheat flour, buckwheat flour, mung bean flour and other flours. Noodles were made by pressing or cutting method. Pressing method used a mold with a hole. The husk of dumpling was made by wheat flour, buckwheat flour, mixed flour of wheat and buckwheat, sliced fish or meat, and/or a gray mullet. Another special method was 'Sangwha' made by fermentation. Bun stuffing of dumpling used fishes, meat, vegetables, beans, flours and seasonings. Cooking methods of dumpling were boiling, baking, steaming and pan-frying. From this investigation of the Korean main dishes described in the Korean literature before the 17th century, we expect to develop useful recipes for those who are concerned about health and want longevity, and thereby to advance the Korean food culture.

Studies on Sheath Rot of Rice Caused by Acrocylindrium oryzae Sawada -Testing Varietal Reactions and Culture Filtrates of the Causal Fungus - (벼 잎집썩음병에 관한 연구 -품종저항성검정, 병균의 배양여액-)

  • Chung Hoo Sup
    • Korean journal of applied entomology
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    • v.14 no.1 s.22
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    • pp.23-27
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    • 1975
  • Occurrence of sheath rot rice caused by Acrocylindrium oryzae on indica varieties, IR lines and IR varieties was light to moderate in the field, whereas generally none of the recommended japonica varieties showed the disease. In general, percentage infection in the field agreed with that obtained from seed inoculation tests made in the laboratory. Culture filtrates of the fungus inhibited the sprouting of seeds of rice, barley, wheat, rye and rape. A new variety, Tongil obtained from a hybrid between japonica and indica was inhibited significantly more than was Jinheung. Conidial germination of Pyricularia oryzae was inhibited by the filtrate while no inhibitory effects were found on conidia of Cochliobolus miyabeanus.

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Two-Dimensional Electrophoretic Analysis of Rice Seed Proteins (쌀 종자 단백질의 2차원 전기영동적 분석)

  • Yoon, Hye-Hyun;Kim, Seung-Ho
    • Applied Biological Chemistry
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    • v.32 no.2
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    • pp.85-90
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    • 1989
  • High resolution two-dimensional (2-D) electrophoresis with isoelectrofocusing in the first dimension and electrophoresis in sodium dodecyl sulfate in acrylamide gradient gels in the second dimension has been used to produce maps of proteins, extracted from rice seeds with 2% sodium dodecyl sulfate/5% 2-mercaptoethanol. Six rice cultivars-three Japonica types and three Tongil(high-yielding) types-at six maturities were studied. Composite map was constructed and more than 300 polypeptide spots were counted in the pH range of $5.2{\sim}8.3$ and molecular range of $20,000{\sim}100,000$. Vast differences were observed between varieties and between maturities in the maps.

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Studies on the Induction of Transformation in Cereal Plants. III. Cultures and Regeneration of Rice Protoplasts Transferred Foreign Genes. (곡물류의 형질전환 유도에 관한 연구 III. 외래 유전자가 도입된 벼 원형질체의 배양 및 재분화)

  • Hwang, Baik;Hwang, Sung-Jin;Im, Hyong-Tak;Kang, Young-Hee
    • KSBB Journal
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    • v.8 no.1
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    • pp.62-68
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    • 1993
  • Transformed rice plantlet were recovered from protoplasts by electroporation with the plasmld pB 1121, which contain the plant expressible NPT-II and GUS genes. Embryonic cell suspension culture was established with embryonic callus induced from mature seeds of rice (Oryza sativa L. cv. Dong-jin) on the MS medium supplemented with 2.0 mg/l 2,4-D, 0.5 mg/l kinetin, 3% sucrose. Protoplasts isolated from embryonic cell suspensions were electroplated and then poterltialty-transformed tissues were selected by growth on the medium containing 200 mg/l kanamycin sulfate. When subjected to GUS assay, they stained blue, indicating the expression of the inserted GUS genes. Plantlets were regenerated from electroplated protoplasts on the hormone free MS medium. Transferred foreign genes in the plants were confirmed by southern hybridization. These results support use of electroporation for transformation of these important cereal plants.

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