Applied Biological Chemistry
- Volume 32 Issue 2
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- Pages.85-90
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- 1989
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
Two-Dimensional Electrophoretic Analysis of Rice Seed Proteins
쌀 종자 단백질의 2차원 전기영동적 분석
- Yoon, Hye-Hyun (Korea Food Research Institute) ;
- Kim, Seung-Ho (Korea Food Research Institute)
- Published : 1989.06.30
Abstract
High resolution two-dimensional (2-D) electrophoresis with isoelectrofocusing in the first dimension and electrophoresis in sodium dodecyl sulfate in acrylamide gradient gels in the second dimension has been used to produce maps of proteins, extracted from rice seeds with 2% sodium dodecyl sulfate/5% 2-mercaptoethanol. Six rice cultivars-three Japonica types and three Tongil(high-yielding) types-at six maturities were studied. Composite map was constructed and more than 300 polypeptide spots were counted in the pH range of
다수확계(통일벼) 및 일반계 각각 3가지 품종의 각 출수후 6시기의 쌀종자에서 2% sodium dodecyl sulfate/5%
Keywords