• Journal of Plant Biotechnology
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• v.48 no.4
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• pp.255-263
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• 2021

Paederia foetida L. is an important medicinal plant that has been used for the treatment of various gastrointestinal related ailments by different tribal communities in India. This plant is also known for its use as a food. Due to overexploitation, P. foetida has been classified as a vulnerable plant in some states of India. The propagation of P. foetida by conventional methods is easy but very slow. Synthetic seed technology offers incredible potential for in vitro propagation of threatened and commercially valuable plants, and can also facilitate the storage and exchange of axenic plant material between laboratories. However, synthetic seed production for P. foetida has not yet been reported. Thus, to the best of our knowledge, the present study is the first attempt to produce synthetic seeds of P. foetida by calcium alginate encapsulation of in vitro regenerated axenic nodal segments. Sodium alginate (3%) and CaCl₂ (100 mM) were found to be the optimal materials for the preparation of ideal synthetic seeds, both in terms of morphology and germination ability. The synthetic seeds showed the best germination (formation of both shoot as well as root; 83.3%) on ½ MS medium augmented with 0.5 mg/L indole-3-acetic acid. The plantlets obtained from these synthetic seeds could be successfully acclimatized under field conditions. We also studied the storage of these synthetic seeds at low temperature and their subsequent sprouting/germination. The seeds showed a germination rate of 63.3% even after 21 days of storage at 4 ℃; thus, they could be useful for transfer and exchange of P. foetida germplasm.

• Journal of the Korean Chemical Society
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• v.25 no.1
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• pp.38-43
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• 1981

Pyrrolidone-anilide derivatives from L-glutamic acid and anilines were synthesized as follows: The products were identified by elementary analysis, IR, NMR and Mass spectra with (R)-2-pyrrolidone-5-carbox-anilide, (R)-2-pyrrolidone-5-cabox-p-chloroanilide, (R)-2-pyrrolidone-5-carbox-o-toluidide, (R)-2-pyrrolidone-5-carbox-m-toluidide and (R)-2-pyrrolidone-5-carbox-p-toluidide. The products were testes for their phytotoxicity on the germination and the seedling growth of radish and rice plants. Among them, (R)-2-pyrrolidone-5-carbox-anilide and (R)-2-pyrrolidone-5-carbox-p-chloroanilide derivatives were strongly inhibitory especially on the germination and the seedling growth of radish seeds. All the compounds also showed an inhibitory activity upon the germination of rice seeds. Additionally, the inhibiting rate of radish growth differs according to the isomeric position(ortho, meta and para) of the methyl group; (R)-2-pyrrolidone-5-carbox-m-toluidide derivative was more effective than both (R)-2-pyrrolidone-5-carbox-o-toluidide and (R)-2-pyrrolidone-5-carbox-p-toluidide derivatives.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.11 no.1
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• pp.63-68
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• 2013

To see if slaked lime and organic fertilizer applications to soil are useful as countermeasures for reducing $^{99}Tc$ concentrations in rice seeds after $^{99}Tc$ contamination of paddy fields, pot experiments were performed for two different paddy soils in a greenhouse. The upper soils for a depth of about 20 cm were treated with the agricultural materials and $^{99}Tc$ 15 d before transplanting. The effects were compared using the transfer factor (TF) defined as the ratio of the plant concentration to the soil concentration. In the case of control plants, TF values for brown rice in the two soils were $4.1{\times}10^{-4}$ and $4.3{\times}10^{-4}$. Of various types of the application, only the application of slaked lime at a lower dose (about 0.6 kg $m^{-2}$), which led to a 60% reduction in the TF value for one soil, seemed to be worth using as a countermeasure. Little effect of the same application was found in the other soil so it is important to determine the effect averaged for a number of soils. Organic fertilizer applications at both of two different doses increased the TF value. It is considered necessary to perform experiments for slake lime applications at doses lower than the above.

• Journal of Plant Biotechnology
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• v.38 no.4
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• pp.251-257
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• 2011

Rice is the most important crop as a model plant for functional genomics of monocotyledons. Rice is usually transformed using Agrobacterium tumefaciens. However, the transformation efficiency using previous method is still low. In this study, we established a new method by modifying the general Agrobacterium protocol especially in the inoculation and co-cultivation step. We directly inoculated Agrobacterium containing a CIPK15 gene under the control of CaMV 35S promoter and NOS terminator in the pCAM1300 vector into the pre-soaked seeds in N6D media for 24 hours. After 7 days of culture at $25^{\circ}C$, calli were formed on seeds cultured on the co-cultivation medium containing an antioxidant compound (1 mM dithiothreitol) and of Agrobacterium growth-inhibiting agent (3 mg/L silver nitrate). We obtained 35 and 22 transgenic plants in rice cultivars, Gopumbyeo and Ilpumbyeo, with increase of transformation efficiency by 30.4% and 22.6%, respectively compared to the general transformation method. The new method in this study would lead to reduction of substantial labor and time to generate transgenic plants.

• Journal of The Korean Society of Grassland and Forage Science
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• v.40 no.2
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• pp.73-79
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• 2020

This study was conducted to develop a technique for the stable production of Italian ryegrass(IRG) seeds in reclamation sites. Harvesting 35 days after heading in Autumn resulted in the highest production, an average of 2,232kg/ha. The production yield decreased due to seed loss as harvesting was delayed to 45 and 55 days post-heading. For the harvested 35 days post-heading, under growing rice sowing resulted in 80% seed yield of after rice sowing's; spring sowing resulted in 40%. After rice sowing using the domestic IRG seeds of 30kg/ha produced the highest seed yield of 2,507kg/ha. The highest straw yield has resulted when using the imported IRG seeds of 20kg/ha for after rice sowing 35 days post-heading fresh weight 36,667kg/ha, dry weight 14,500kg/ha, and TDN weight 7,895kg/ha.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.6
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• pp.766-777
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• 1997

The purpose of this study was to investigate inheritance modes of low temperature germinability(LTG) on rice. Two experiments were carried out: In experiment I, the seeds of 10 rice varieties yielded at the different cultivation conditions which were transplanted in a paddy field from May 20 to July 5 with 15-day interval, were tested at fixed temperature (13$^{\circ}C$) in-door and at field with natural conditions to find out the change of LTG according to different cultivating condition among the each variety, In experiment II, F$_1$ seeds of full diallal cross from six parents, were analyzed for combining ability and reciprocal differences of low temperature germinability. The results are summarized as follows. The seeds of 10 varieties showed significant differences of LTG at the different cultivation conditions, varieties and their interaction. And the relationship between LTG coefficient at 13$^{\circ}C$ and low temperature emergence(LTE) coefficient at field was highly significantly positive(r=0.511). From the analysis of the diallel cross, high significance was found at reciprocal difference and alpha inheritance. The mean squares of general combining ability(GCA) and specific combining ability of LTG were highly significant with higher mean squares of GCA. Seventeen out of 30 cross combinations showed transgressive segregation.

• Journal of Sasang Constitutional Medicine
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• v.13 no.3
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• pp.59-74
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• 2001

In oriental medicine, food are regarded as important one like medicine. Especially Sasang medicine put emphasis on diet, it regarded food as a important one by taking food according to their own constitution in the point of improving health condition and preventing diseases. In this report, the author try to find out the relationship between food preference and each suitable food according to Sasang constitution by using survey data. The results are like this. 1. In constitutional distribution patterns among 203 person(male : 118 person, female : 85 person) reveal Soyang Group 58person(28.6%), Taeum Group 61(30%) and Soeum Group 84(41.4%). And their were no significant difference in their height in all group according to gender. Compared with the other groups, When compared with the other groups on body weight and BMI, Taeum Group indicate significantly high body weight and BMI(Body Mass Index) in both gender. 2. Taeum Group show significantly high smoking rate. But alcohol drinking rates reveal no significant difference in all groups. 3. In the Survey about food preference according to the constitution, rice, Soju, watermelon show significantly high preference in case of suitable food to each group. But perilla seeds, coffee, ginger tea show insignificant results. 4. In the survey about food preference according to the constitution and sex, welsh onion, crab preserved with soysauce, beer show significantly high preference in case of suitable food to each group. But glutinous rice, cooked barley, water dropwort muchim, lettuce, dog meat, egg, yellow croaker, coffee, ginger tea, and Soju show insignificant results. 5. In the survey about food preference according to the constitution and age, rice, watermelon, onion, garlic, salt and Soju show significantly high preference in case of suitable food to each group. But sugar and perilla seeds show insignificant results. 6. In the survey about food preference according to constitution, sex and age, glutinous rice, soybean milk, banana, crab preserved with soysauce, sea cucumber, sea mustard, Soju, beer, onion, garlic, salt show high preference in case of suitable food to each group. But cooked barley, sugar, water dropwort muchim, dog meat, puffer soup and perilla seeds show insignificant results.

• Journal of Ginseng Research
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• v.11 no.1
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• pp.66-76
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• 1987

When rice seeds were rinsed in If) Korean ginseng tea (KG 722, Korean Ginseng Product Co. Ltd. made) for 28 hours prior to seeding, the early growth was found relatively fast and their resistance against blight and harmful insects was increased. Rice yield showed that control group, rinsing group and spraying group were 514 kg/10a, 562 kg/ 10a and 571 kg/ 10a respectively. However, rinsing and spraying groups were 590 kg/ 10a (leaf spraying) and 605 kg/ 10a (spray immediate after seeding) respectively.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.4
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• pp.348-355
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• 2006

Phenotypes of panicle, hull and seed of mutant rice (Oryza sativa L.) were characterized. Panicle mutants were classified in 4 groups with their internode length of main rachis, primary rachis, secondary rachis and pedicel. Hull and seed mutants were grouped into 12 based on their mutant characters in shape, size and color of seeds. These natural and spontaneous mutant collections showed distinct phenotypes to wild type rice. This might be useful for the identification of the functions of genetic factors in the Mendelian inheritance.

• Journal of Plant Biology
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• v.37 no.3
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• pp.293-299
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• 1994

Developing rice endosperm cells display two morphologically distinct rough endoplasmic reticulum (ER) membranes, the cisternae ER (C-ER) and theprotein body ER (PB-ER), the latter delimiting the prolamine protein bodies. We (Li et al., 1993) have recently shown that the storage protein mRNAs are not randomly distributed on these ER types; the C-ER is enriched for glutelin mRNAs, whereas the PB-ER harbors predominantly prolamine transcripts. To address whether these ER types have differnet capacities to translate these mRNAs and translocate their proteins into the lumen, a microsomal fraction enriched in C-ER vesicles was prepared from devleoping rice seeds. When present in an in vitro translatin system, the microsomes were able to proteolytically remove the signal peptide and internalize both preproglutelin and preprolamine within the microsomal vesicles. Of the two species, preprolamine was more effectively translocated and processed. These results suggest that the C-ER has the capacity to recognize and bind both storage protein mRNAs during protein synthesis. Moreover, efficient translocation and processing of glutelin requires additional factors that are deficient or absent in the in vitro system.