• Journal of KIISE:Information Networking
• /
• v.37 no.3
• /
• pp.167-174
• /
• 2010

Recently the distributed topology control algorithm for constructing the Recrystallization Topology in the unstructured peer-to-peer network was proposed. In this paper, we prove that such a hierarchical topology is a scale-free power-law network. We present a model of a construction process of the distributed protocol, and analyze it based on a mean-field approximation and the continuum theory, so that we show that the constructed Recrystallization Topology is a scale-free network. In the proposed model, all nodes are born with some initial attractiveness and the system incorporates the rewiring of some links at every time step. Some old links are removed with the anti-preferential probability, and some new links are added with preferential probability. In other words, according to the distributed algorithm, each node makes connections to the more-preferential nodes having higher hit-ratio than other nodes, while it disconnects the anti-preferential nodes having lesser hit-ratio. This gives a realistic description of the local processes forming the recrystallization topology in unstructured peer-to-peer network. We calculate analytically the degree distribution. The analytic result indicates that the constructed network is a scale-free network, of which the scaling exponent is 3.

• Molecules and Cells
• /
• v.38 no.4
• /
• pp.373-379
• /
• 2015

Pyruvate kinase M2 isoform (PKM2), a rate-limiting enzyme in the final step of glycolysis, is known to be associated with the metabolic rewiring of cancer cells, and considered an important cancer therapeutic target. Herein, we report a novel PKM2 activator, PA-12, which was identified via the molecular docking-based virtual screening. We demonstrate that PA-12 stimulates the pyruvate kinase activity of recombinant PKM2 in vitro, with a half-maximal activity concentration of $4.92{\mu}M$, and effectively suppresses both anchorage-dependent and -independent growth of lung cancer cells in non-essential amino acid-depleted medium. In addition, PA-12 blocked the nuclear translocalization of PKM2 in lung cancer cells, resulting in the inhibition of hypoxia response element (HRE)-mediated reporter activity as well as hypoxia-inducible factor 1 (HIF-1) target gene expression, eventually leading to the suppression of cell viability under hypoxia. We also verified that the effects of PA-12 were dependent on PKM2 expression in cancer cells, demonstrating the specificity of PA-12 for PKM2 protein. Taken together, our data suggest that PA-12 is a novel and potent PKM2 activator that has therapeutic implications for lung cancer.

• Journal of Chest Surgery
• /
• v.43 no.4
• /
• pp.381-386
• /
• 2010

Background: Sternal wound infection (SWI) is an important complication after cardiac surgery. The aim of this study was to investigate the predictors affecting sternal wound infection and preventive factors including short term Vancomycin therapy in patients who underwent coronary artery bypass grafting (CABG). Material and Method: A retrospective study was done using data collected from January 2001 through December 2007. This included 219 patients who had isolated CABG. The definition of SWI was documentation from a microbiological study and a requirement for simple closure or other surgical revision. Result: The overall incidence of SWI was 7.8% (n=17). The causative organisms were methicillin resistant Staphylococcus aureus (MRSA, n=13), methicillin resistant Staphylococcus epidermidis (n=2), Pseudomonas aeruginosa (n=1), and Candida albicans (n=1). Ten cases had deep sternal wound infection with mediastinitis; 7 cases had a superficial wound infection. Infection-related mortality was low (1/17; 6%). Diabetes mellitus (p=0.006) and smoking history (p=0.020) were factors that predicted high risk. Short term use of vancomycin decreased the incidence of MRSA-associated SWI (p=0.009). For treatment, curettage and rewiring or flap were needed in most cases (88%, n=14). Conclusion: Patients who had diabetes mellitus and a smoking history need careful management. Short term use of vancomycin is effective for prevention of SWI with MRSA.