• 한국세라믹학회지
• /
• 제30권9호
• /
• pp.731-739
• /
• 1993

R-curve measurements were performed on Al2O3(matrix)-ZrO2-SiC whisker composite and Al2O3-ZrO2, Al2O3-SiC whisker composites in the favor of comparing the effect of ZrO2 and SiC whisker, as a second phase, to Al2O3 matrix. Al2O3-SiC whisker and Al2O3-ZrO2-SiC whisker were fabricated by hot pressing at 1$700^{\circ}C$, 15MPa and Al2O3-ZrO2 by pressureless sintering at 1$600^{\circ}C$. A controlled flaw/strength technique was utilized to determine fracture resistance as a function of crack extension and R-curve behaviour was determined from the relationship which is KR=K0(Δa)m. R-curveresults were KR=6.173$\times$Δa0.031 for Al2O3-ZrO2, KR=18.796$\times$Δa0.172 for Al2O3-SiC whisker and KR=11.96$\times$Δa0.110 for Al2O3-ZrO2-SiC whisker composite. From the analysis of R-curve and expeirmental data above three composites, it is found that R-curve behaviour of Al2O3-ZrO2-SiC whisker composite was dominated initially by the strengthening effect of ZrO2 and after, some extended crack were influenced by the effect of SiC whisker. Analysis of SEM and X-ray data revealed that whisker bridging in the crack wake and whisker pull-out mechanisms were the main mechanism for the R-curve behaviour.

• 전자공학회논문지
• /
• 제51권10호
• /
• pp.204-209
• /
• 2014

본 논문은 맥파전달현상을 유지하며 맥압을 조절할 수 있는 시뮬레이터를 제작하는 것이 목적이다. 이를 위해 탄성튜브와 컴플라이언스 챔버를 융합하였다. 본 시뮬레이터는 슬라이더-크랭크로 구성된 압력발생부, 저항조절부를 포함한 혈관부, 수조 그리고 측정부로 구성되어있다. 챔버의 위치선정을 위해 챔버의 위치에 따른 맥파의 변화를 실험하였다. 또한 챔버가 맥압에 미치는 영향을 보기 위해 챔버의 유무에 따른 맥압의 변화를 비교하였다. 챔버의 유무가 맥파전달현상에 미치는 영향을 조사하였다. 실험결과, 튜브의 상류지점에 챔버를 설치할 때가 하류지점에 설치할 때 보다 인체와 더 유사한 압력파형을 나타냈다. 챔버를 설치하였을 때가 설치하지 않았을 때보다 인체와 유사한 맥압을 생성하였다. 챔버의 설치여부에 따른 맥파 전달 속도는 큰 변화가 없었다.

• BMB Reports
• /
• 제51권6호
• /
• pp.296-301
• /
• 2018

Mitochondrial DNA (mtDNA) mutations are often observed in various cancer types. Although the correlation between mitochondrial dysfunction and cancer malignancy has been demonstrated by several studies, further research is required to elucidate the molecular mechanisms underlying accelerated tumor development and progression due to mitochondrial mutations. We generated an mtDNA-depleted cell line, ${\rho}^0$, via long-term ethidium bromide treatment to define the molecular mechanisms of tumor malignancy induced by mitochondrial dysfunction. Mitochondrial dysfunction in ${\rho}^0$ cells reduced drug-induced cell death and decreased the expression of pro-apoptotic proteins including p53. The p53 expression was reduced by activation of nuclear $factor-{\kappa}B$ that depended on elevated levels of free calcium in $HCT116/{\rho}^0$ cells. Overall, these data provide a novel mechanism for tumor development and drug resistance due to mitochondrial dysfunction.

• 한국공간정보시스템학회:학술대회논문집
• /
• 한국공간정보시스템학회 2004년도 춘계 학술발표회 논문집 제1권1호(통권1호)
• /
• pp.75-82
• /
• 2004

Many researcher's efforts have made a significant advancement of space frame structure with various portion, and it becomes the most outsanding one of space structures. However, with the characteristics of thin and long term of spacing, the unstable behavior of space structure is shown by initial imperfection, erection procedure or joint, especially space frame structure represents more. This kind of unstable problem could not be set up clearly and there is a huge difference between theory and experiment. Moreover, the discrete structure such as space frame has more complex solution, this it is not easy to derive the formulation of design about space structure. In this space frame structure, the character of rise-span ratio or load mode is represented by the instability of space frame structure with initial imperfection, and snap-through or bifurcation might be the main phenomenon. Therefore, in this study, space frame structure which has a lot of aesthetic effect and profitable for large space covering single layer is dealt. And because that the unstable behavior due to variation of inner force resistance in the elastic range is very important collapse mechanism, I would like to investigate unstable character as a nonlinear behavior with a geometric nonlinear. In order to study the instability. I derive tangent stiffness matrix using finite element method and with displacement incremental method perform nonlinear analysis of unit space structure, star dome and 3-ring star dome considering rise-span $ratio(\mu}$ and load $ratio(R_L)$ for analyzing unstable phenomenon.

• 생명과학회지
• /
• 제15권1호
• /
• pp.112-117
• /
• 2005

하천변에 사는 식물들은 침수 저항성을 갖추고 있는데 그중 Ranunculus속이나 Rumex속에 속하는 일부 반수생 식물들에서 볼 수 있는 것처럼, 엽병이 급격한 생장을 일으켜 잎이 수표면에 이르게 함으로써 호흡할 수 있는 조건을 확보하는 현상은 식물체내에 축적된 에틸렌에 의하여 매개되는 현상이다. 침수 조건에서는 식물 조직 내에 에틸렌이 축적되며, 축적된 에틸렌은 옥신 등 다른 식물호르몬에 대한 조직의 감수성을 증가시켜 생장을 촉진한다. 증가된 생장으로 잎이 수표면에 이르게 되면 다시 공기를 접촉하여 호흡할 수 있게 되고, 식물체내에 축적되었던 에틸렌은 대기 중으로 확산하여 나가게 되므로 체내 에틸렌 농도가 감소하여 생장률이 침수 이전 수준으로 되돌아간다. 에틸렌에 의한 옥신의 감수성 증가는 선량 반응 곡선에 비추어 수용체의 친화도 증가가 수반되는 것으로 생각되는데, 아직 옥신 수용체가 밝혀지지 않아 분자생물학적 기작은 연구되어야 할 과제로 남아있다. 최근 옥신 수용체 발견의 가능성이 높아지고 있으며, 따라서 에틸렌에 의한 옥신 수용체의 옥신에 대한 감수성 조절기작을 탐구할 수 있는 길이 열릴 것으로 기대된다.

• Asian Pacific Journal of Cancer Prevention
• /
• 제17권3호
• /
• pp.1175-1179
• /
• 2016

The results of this study demonstrate the potential prognostic and predictive values of KRAS and BRAF gene mutations in patients with colorectal cancer (CRC). It has been proven that KRAS and BRAF mutations are predictive biomarkers for resistance to anti-EGFR monoclonal antibody treatment in patients with metastatic CRC (mCRC). We demonstrated the distribution of KRAS (codons 12, 13 and 61) and BRAF (codon 600) gene mutations in 50 mCRCs using direct sequencing and compared the results with clinicopathological data. KRAS and BRAF mutations were identified in 15 (30%) and 1 (2%) patients, respectively. We identified KRAS mutations in codon 12, 13 and 61 in 73.3% (11/15), 20% (3/15) and 6.67% (1/15) of the positive patients, respectively. The KRAS mutation frequency was significantly higher in tumors located in the ascending colon (p=0.043). Thus, we found that approximately 1/3 of the patients with mCRC had KRAS mutations and the only clinicopathological factor related to this mutation was tumor location. Future studies with larger patient groups should yield more accurate data regarding the molecular mechanism of CRC and the association between KRAS and BRAF mutations and clinicopathological features.

• Asian Pacific Journal of Cancer Prevention
• /
• 제16권9호
• /
• pp.3907-3912
• /
• 2015

Purpose: To investigate the antitumor activity and mechanism of chloroquine (CQ) in combination with cisplatin (DDP) in nude mice xenografted with gastric cancer SGC7901 cells. Materials and Methods: 35 cases of gastric cancer patients with malignant ascites were enrolled and intraperitoneal cisplatin injection was performed. Ascites were collected before and 5 days after perfusion for assessment of autophagy levels in cancer cells. In addition, 24 tumor-bearing mice were randomly divided into control, DDP, CQ and CQ + DDP groups. Results: In 54.3% (19/35) of patients the treatment was therapeutically effective (OR), 5 days after peritoneal chemotherapy, 13 patients had the decreased ascites Beclin-1 mRNA levels. In 16 patients who had NR, only 2 cases had decreased Beclin-1 (P=0.001). Compared with the control group, the xenograft growth in nude mice in the DDP group was low, and the inhibition rate was 47.6%. In combination with chloroquine, the inhibition rate increased to 84.7% (P<0.01). The LC3-II/I ratio, and Beclin1 and MDR1/P-gp expression were decreased, while caspase 3 protein levels increased (P<0.05). Conclusions: Antitumor ability of cisplatin was associated with autophagy activity and chloroquine can enhance chemosensitivity to cisplatin in gastric cancer xenografts nude mice.

• Asian Pacific Journal of Cancer Prevention
• /
• 제17권3호
• /
• pp.1285-1292
• /
• 2016

Background: It is well established that mutations in the BRCA1 gene are a major risk factor for breast cancer. Induction of cancer cell death and inhibition of survival are the main principles of cancer therapy. In this context, autophagy may have dual roles in cancer, acting on the one hand as a tumor suppressor and on the other as a mechanism of cell survival that can promote the growth of established tumors. Therefore, understanding the role of autophagy in cancer treatment is critical. Moreover, defects in apoptosis, programmed cell death, may lead to increased resistance to chemotherapy. Purpose: The aim of the present study was to detect BRCA1 gene mutations in order to throw more light on their roles as risk factors for breast cancer in Egypt. Secondly the role of autophagy and apoptosis in determining response to a fluorouracil, doxorubicin, cyclophosphamide (FAC) regimen was investigated. Materials and Methods: Forty-five female breast cancer cases and thirty apparently healthy females were enrolled in the present study. Serum levels of autophagic biomarkers, Beclin 1 and LC3 as well as the serum levels of apoptosis biomarkers Bcl-2 and Caspase-3 were measured before and after chemotherapy. Results: BRCA1 mutations were found in 5 (16.7%) and 44 (99.8%) of the controls and cancer patients, the most frequent being 5382insC followed by C61G and 185 delAG. The results revealed that chemotherapy caused elevation in serum concentration levels of the autophagic biomarkers (Beclin 1 and LC3). This elevation was associated with a significant decrease in serum concentration levels of Bcl-2 and significant increase in caspase-3 concentration levels (apoptotic markers). Conclusions: The results of the present study indicate a very high level of BRCA mutations in breast cancer cases in Egypt and point to involvement of autophagic and apoptotic machinery activation in response to FAC chemotherapy.

• 전자공학회논문지
• /
• 제50권6호
• /
• pp.167-172
• /
• 2013

최근 Biswas등은 신원기반의 대리서명을 사용한 차량통신망 메시지 인증 프로토콜을 제안하였다. 저자들은 제안된 인증기법이 대리 서명키에 대한 안전성, 메시지 위조 및 재전송 공격에 대한 안전성, 부인방지 서비스, 대리 서명키 노출에 대한 저항성 등에 대한 안전성을 제공한다고 주장하였다. 본 논문에서는 Biswas등이 제안한 프로토콜에서 위임 받지 않은 임의의 공격자가 원 서명자의 비밀키에 대한 정보 없이 메시지 전달자의 대리 서명키를 계산할 수 있음을 보인다. 이것은 Biswas등이 제안한 메시지 인증 프로토콜은 저자들의 주장과는 달리 안전하지 않음을 의미한다. 또한, 본 논문에서는 이를 해결할 수 있는 개선된 프로토콜을 제시한다.

• 한국인터넷방송통신학회논문지
• /
• 제12권6호
• /
• pp.83-89
• /
• 2012

본 논문에서는 RFID 시스템에서 개인 정보보호를 위해 보안성이 강화된 인증 프로토콜의 설계를 제안한다. 제안된 방법에서는 AES(Advanced Encryption Standard) 암호 알고리듬을 기반으로 하여 3중 CRA(challenge response authentication) 방식을 사용한다. 또한, 개선된 인증 메카니즘의 실현을 위해 기존의 ISO/IEC 18000-3 표준을 수정한 3종류의 프로토콜 프레임 패킷 형식을 제안한다. 보안성 비교를 통하여 제안한 알고리듬이 보다 보안성이 강인함을 제시하였으며, 제안한 프로토콜의 검증을 위해 RFID Tag을 위한 디지털 Codec을 설계하였다. 설계된 Codec은 Verilog HDL을 사용하였고, Xilinx Virtex XCV400E device를 사용하여 합성을 수행하였다. 시뮬레이션 결과를 통하여 제안한 프로토콜이 안정성 향상과 함께 정확히 동작함을 보였다.