• 제목/요약/키워드: Research synthesize

검색결과 498건 처리시간 0.03초

Affinity Immobilization of Dextransucrase on Dextran-based Support and the Production of Leucrose

  • Han, Nam-Soo;Kang, Seung-Yeon;Lee, Soo-Bok;Robyt, John F.
    • Food Science and Biotechnology
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    • 제14권3호
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    • pp.317-322
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    • 2005
  • A simple and convenient method of immobilizing dextransucrase via an affinity interaction is described, along with the use of this system to synthesize leucrose. Dextransucrase was produced in sucrose-free medium by fermenting a constitutive mutant of Leuconostoc mesenteroides NRRL B-512F and was separated using an ultrafiltration membrane. The purified enzyme was free of dextran polymer, which previously was always found with the sucrose-induced enzyme. Therefore, it was possible to immobilize the enzyme on dextran-based resins using an affinity interaction. Sephadex G-200 was the best resin for immobilizing the dextransucrase and gave a fast flow rate through the packed column. The immobilized dextransucrase retained more than 80% of its specific activity after immobilization ($K_m\;=\;18.1\;mM$ and $k_{cat}\;=\;450\;sec^{-1}$ vs. 13.1 mM and $640\;sec^{-1}$, respectively, for the free enzyme). The immobilized dextransucrase showed improved stability over a pH range of 4.0 to 6.5 and at moderately high temperatures over $40^{\circ}C$. When immobilized dextransucrase was used to synthesize leucrose via the transfer reaction with sucrose and fructose, about 74% of the sucrose was converted into leucrose after one day, and the half-life of the enzyme activity was 15 days. Regeneration of the resin by supplementation with dextransucrase enabled the recovery of the initial activity of the system, but both the reaction and the flow rate were lower, probably owing to the accumulation of dextran inside the resin.

M&A 협상성과에 기업간 문화차이가 미치는 영향에 관한 연구 (An Approach on the Global Commerce Activation Regarding Culture Collision by the Use of M&A)

  • 박종돈
    • 통상정보연구
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    • 제8권2호
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    • pp.229-247
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    • 2006
  • The company culture is becoming usual term in business management field. That organization culture of these phenomenon becomes resource of company competitive power indeed, it is basing to logic for important leading person for elevation with systemicity. also, great many scholars do research to subject that is change management of company administration and there are not much example that companies succeed in change actually despite had announced treatise etc. This research tries to synthesize several research subjects by single model regarding confrontation(culture collision) phenomenon between culture that can happen in company through literature research and example analysis basically. Specially, try to classify and divide this by specific developing and arrange in each type with using much this culture analysis in negotiation analytics. Through this problem and suggestion point by this confrontation way in this culture negotiation side and culture risk management side of administration make try to.

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Purification and Properties of Extracellular Esterases of Aspergillus oryzae which synthesize Ethyl Caproate

  • Lee, Jong-Hoon;Sato, Toshitsugu;Kawai, Yuri;Enei, Hitoshi
    • Journal of Microbiology and Biotechnology
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    • 제5권5호
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    • pp.274-279
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    • 1995
  • Ethyl caproate, one of the major flavor compounds deciding the quality of sake (Japanese wine), is produced during the brewing by the action of alcohol acyltransferase and esterases of sake yeast and koji mold. Extracellular esterases of Aspergillus oryzae required for ethyl caproate synthesis were purified partially. The enzymes had different optimum pH and affinity toward substrates. Substrate preferences and inhibition features showed the three enzymes to be B-type esterases or carboxylesterases (EC 3.1.1.1).

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The Novel Functional Chromophores Based on Squarylium Dyes

  • Park, Soo-Youl;Jun, Kun;Oh, Sea-Wha
    • Bulletin of the Korean Chemical Society
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    • 제26권3호
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    • pp.428-432
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    • 2005
  • Squarylium or squaraine dyes are derived from 1,2-dihydroxycyclobuten-3,4-dione, otherwise known as squaric acids. They are two principal types: the 1,2-bisdonorsubstituted derivatives, and the 1,3-bisdonorsubstituted derivatives. The former are essentially merocyanines and have no distinctive properties, whereas the latter represent a unique type of chromophore, which is neither a merocyanines nor cyanine and has exceptional light absorption characteristics. They also have many functional applications based on their special properties. Thus it was the objective of this research project to synthesize a range of 1,3-squarylium dyes of widely differing structural types, and to investigate their light absorption and fluorescence properties in general, and the color change properties of appropriate examples in particular. Also in this study, the various pHinduced colour change processes were examined.

Protective Coatings for Accident Tolerant Fuel Claddings - A Review

  • Rofida Hamad Khlifa;Nicolay N. Nikitenkov
    • 방사성폐기물학회지
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    • 제21권1호
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    • pp.115-147
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    • 2023
  • The Fukushima accident in 2011 revealed some major flaws in traditional nuclear fuel materials under accidental conditions. Thus, the focus of research has shifted toward "accident tolerant fuel" (ATF). The aim of this approach is to develop fuel material solutions that lead to improved reactor safety. The application of protective coatings on the surface of nuclear fuel cladding has been proposed as a near-term solution within the ATF framework. Many coating materials are being developed and evaluated. In this article, an overview of different zirconium-based alloys currently in use in the nuclear industry is provided, and their performances in normal and accidental conditions are discussed. Coating materials proposed by different institutions and organizations, their performances under different conditions simulating nuclear reactor environments are reviewed. The strengths and weaknesses of these coatings are highlighted, and the challenges addressed by different studies are summarized, providing a basis for future research. Finally, technologies and methods used to synthesize thin-film coatings are outlined.

Knowledge Management Research Based on Social Network Theories: A Review with Future Directions

  • Tae Hun Kim
    • Asia pacific journal of information systems
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    • 제32권1호
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    • pp.168-190
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    • 2022
  • This review aims to synthesize social network theories by drawing on the importance of social network perspectives in understanding knowledge management with technology in organizations. I provide an overview of prior social network research with the following core ideas: the primacy of relations between organizational actors, the utility of actors' embeddedness in social fields, the social utility of network connections, and the structural patterning of social life. On top of that, I summarize critical social perspectives (the social capital theory, the structural hole theory, the embeddedness perspective, the social exchange theory, the organizational learning theory, and the innovation diffusion theory) to suggest potential research questions for future studies in social network research in the knowledge management discipline.

수학 학습에 미치는 주요 영향 요인 분석: 선행 연구로부터의 통찰 (Identifying Key Influences on Mathematics Learning: Insights from Prior Research)

  • 김홍겸;고호경
    • East Asian mathematical journal
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    • 제40권2호
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    • pp.231-265
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    • 2024
  • Achieving something in learning is a very important task. Due to its significance, extensive research has been conducted over a long period to determine what factors influence learning. In the field of mathematics, such research has been continuously carried out, and as a result, it has been revealed that cognitive, affective, and socio-environmental factors influence mathematics learning. However, most of these studies were based on one or two variables, and thus, they did not comprehensively examine the factors affecting mathematics learning. Therefore, this study aims to synthesize the existing research to comprehensively derive the factors influencing mathematics learning.

A convenient synthesis of 2′ or 3′-amino-2′(or 3′)-deoxyadenosine and 5′-chloro-2′(or 3′)-amino-deoxyadenosine analogues

  • Kim, Beom-Tae;Kim, Seung-Ki;Hwang, Ki-Jun
    • 대한약학회:학술대회논문집
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    • 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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    • pp.365.3-366
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    • 2002
  • New and improved preparations of structurally modified nucleosides are important goals in synthetic organic chemistry because of the potential utility of these compounds as synthetic precursors of many biologically active molecules in cells. In our program to synthesize the bioactive nucleosides, such as AdoHcy hydrolase inhibitors and cyclic adenosine diphosphoribose(cADPR) analogues. (omitted)

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Solution-Phase Synthesis of Shape-Controlled Metal Nanocrystals

  • 임병권
    • 한국재료학회:학술대회논문집
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    • 한국재료학회 2011년도 춘계학술발표대회
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    • pp.21.1-21.1
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    • 2011
  • Controlling the morphology of a metal nanocrystal is critical to modern materials chemistry because its physical and chemical properties can be easily and widely tuned by tailoring the size and shape. Combined with ease of synthesis and processing, metal nanocrystals with desired morphologies and thus properties are promising candidates for a wide variety of applications in catalysis, sensing, imaging, electronics, and photonics, and medicine. In this talk, I would like to introduce my recent research results on the shape-controlled synthesis of metal nanocrystals using a simple aqueous method. This water-based system provides a number of merits such as simplicity, convenience, and the potential for large-scale production and enables us to synthesize metal nanocrystals with a rich variety of shapes such as truncated octahedron, cubes, bars, octahedrons, and thin plates. The ability to control the shape of metal nanocrystals provides a great opportunity to systematically investigate their catalytic and optical properties.

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유연·신축성 전자 소자 개발을 위한 은 나노와이어 기반 투명전극 기술 (Recent Trends in Development of Ag Nanowire-based Transparent Electrodes for Flexible·Stretchable Electronics)

  • 김대곤;김영민;김종웅
    • 마이크로전자및패키징학회지
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    • 제22권1호
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    • pp.7-14
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    • 2015
  • Recently, advances in nano-material researches have opened the door for various transparent conductive materials, which include carbon nanotube, graphene, Ag and Cu nanowire, and printable metal grids. Among them, Ag nanowires are particularly interesting to synthesize because bulk Ag exhibits the highest electrical conductivity among all metals. Here we reviewed recently-published research works introducing various devices from organic light emitting diode to tactile sensing devices, all of which are employing AgNW for a conducting material. They proposed methods to enhance the stretchability and reversibility of the transparent electrodes, and apply them to make various flexible and stretchable electronics. It is expected that Ag nanowires are applicable to a wide range of high-performance, low-cost, stretchable electronic devices.