• Journal of Microbiology and Biotechnology
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• 제25권7호
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• pp.1015-1025
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• 2015

The development of diverse polymyxin derivatives is needed to solve the toxicity and resistance problems of polymyxins. However, no platform has generated polymyxin derivatives by genetically engineering a polymyxin synthetase, which is a nonribosomal peptide synthetase. In this study, we present a two-step approach for the construction of engineered polymyxin synthetases by substituting the adenylation (A) domains of polymyxin A synthetase, which is encoded by the pmxABCDE gene cluster of Paenibacillus polymyxa E681. First, the seventh L-threonine-specific A-domain region in pmxA was substituted with the L-leucine-specific A-domain region obtained from P. polymyxa ATCC21830 to make polymyxin E synthetase, and then the sixth D-leucine-specific A-domain region (A_6-D-Leu-domain) was substituted with the D-phenylalanine-specific A-domain region (A_6-D-Phe-domain) obtained from P. polymyxa F4 to make polymyxin B synthetase. This step was performed in Escherichia coli on a pmxA-containing fosmid, using the lambda Red recombination system and the sacB gene as a counter-selectable marker. Next, the modified pmxA gene was fused to pmxBCDE on the chromosome of Bacillus subtilis BSK4dA, and the resulting recombinant strains BSK4-PB and BSK4-PE were confirmed to produce polymyxins B and E, respectively. We also succeeded in constructing the B. subtilis BSK4-PP strain, which produces polymyxin P, by singly substituting the A_6-D-Leu-domain with the A_6-D-Phe-domain. This is the first report in which polymyxin derivatives were generated by genetically engineering polymyxin synthetases. The two recombinant B. subtilis strains will be useful for improving the commercial production of polymyxins B and E, and they will facilitate the generation of novel polymyxin derivatives.

• ETRI Journal
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• 제36권3호
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• pp.429-438
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• 2014

Semantic role labeling (SRL) is a task in natural-language processing with the aim of detecting predicates in the text, choosing their correct senses, identifying their associated arguments, and predicting the semantic roles of the arguments. Developing a high-performance SRL system for a domain requires manually annotated training data of large size in the same domain. However, such SRL training data of sufficient size is available only for a few domains. Constructing SRL training data for a new domain is very expensive. Therefore, domain adaptation in SRL can be regarded as an important problem. In this paper, we show that domain adaptation for SRL systems can achieve state-of-the-art performance when based on structural learning and exploiting a prior model approach. We provide experimental results with three different target domains showing that our method is effective even if training data of small size is available for the target domains. According to experimentations, our proposed method outperforms those of other research works by about 2% to 5% in F-score.

• East Asian mathematical journal
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• 제32권4호
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• pp.465-482
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• 2016

This study aims to investigate research trends relevant to mathematics teachers and discuss implications on professional development of mathematics teachers. For the purpose of this study, we review 156 articles written between 2006 and 2015 related to mathematics teachers. The articles are analyzed using the model of professional growth suggested by Goldsmith, Doerr, Lewis(2014). The model discusses personal domain, external domain, domain of practice, and domain of consequence. In the results of this study, articles focusing on the personal domain of mathematics teachers appear a lot in reviewed ones, whereas studies relevant to external domain or domain of practice of mathematics teachers are relatively small.

• 한국IT서비스학회:학술대회논문집
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• 한국IT서비스학회 2002년도 추계학술대회
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• pp.425-430
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• 2002

Over the years, several different approaches have been proposed to enhance the practice of software reuse. These approaches vary in reuse abstraction levels or application domains they focus on. However, it seems that they do not adequately meet the needs of software designers in planning and managing reuse. One reason may be the lack of conceptually well-established framework supporting work domain analysis and modeling. As a new viable solution to this problem, Sutcliffe and his colleagues developed Domain Theory , which is introduced in this paper. Utilizing multidisciplinary perspective, such as cognitive psychology, management science, human-computer interaction, and software engineering. it provides a schematic framework that defines a comprehensive library of generic and reusable models of domain knowledge in terms of generic tasks and meta-domains. It also provides useful methods and guidelines for software reuse, emphasizing the concept of abstraction process in a designer s mind. This paper firstly gives a brief overview of fundamentals of software reuse. Next, it explains the foundation of domain theory and discusses its applicability to software reuse. In particular, the taxonomy of meta-domains and the types of generic tasks are described in more detail. Finally, the future research framework, which primarily addresses the problem of how to apply the domain theory to various work domains, is proposed.

• Molecules and Cells
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• 제19권2호
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• pp.223-227
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• 2005

We have identified TMPRSS6, a novel type 2 transmembrane serine protease. TMPRSS6 possesses all the signature motifs of the family of transmembrane serine proteases (TMPRSSs), including a transmembrane domain, an LDL receptor class A (LDLRA) domain, a scavenger receptor cysteine-rich (SRCR) domain, and a serine protease domain. The substrate specificity of TMPRSS6 is slightly different from those of other TMPRSS family members. Combined with the finding that TMPRSS6 is expressed strongly in the thyroid and weakly in the trachea, this may indicate that TMPRSS6 has a specialized role.

• 영재교육연구
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• 제18권2호
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• pp.201-237
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• 2008

본 연구는 과학 및 시각예술 영역의 창의적 성취사례를 통해 두 영역의 영역보편성과 영역특정성에 대해 살펴보는 데 그 목적을 두었다. 연구를 위해 각각의 영역에서 세계적인 성취를 이룬 한국의 창의적 인물들(과학자: 10인, 시각예술가: 9인)에 대한 인터뷰를 실시하였고, 이로부터 수집한 질적 자료를 토대로 창의적 성취에서 영역보편적으로 드러나는 특성과 영역특정적으로 드러나는 특성을 요약, 기술하였다. 자료분석에는 본 연구의 근간이 되는 총괄적이고 체계적인 개념틀 '지식진화시스템(Knowledge-Evolving Systems: KES)'을 사용하였다. 분석 결과를 보면, 실재시스템에서 두 영역의 성취는 공통적으로 요동, 탐색, 산물을 통해 드러났다. 반면, 과학은 전문지식, 어려운 과제, 세계 최초의 객관적인 지식이 주요한 요인으로, 예술에서는 일상적인 지식, 다양한 주제, 새로운 변화에 대한 인간의 감성 전달이 주된 요인으로 드러났다. 개인시스템에서 두 영역은 모두 분명한 목표를 향한 집중과 독자적인 노력을 보편적으로 요구하였다. 반면 과학은 창조의지, 확산적 및 분석적 사고, 직관 및 통찰, 그리고 도전이 대표적인 요인으로, 시각예술은 즐거움, 민감성, 통합적 사고, 완벽성, 그리고 자유로움이 주요한 요인으로 산출되었다. 마지막으로 사회시스템에서 두 영역의 성취는 전문가의 승인에 의해 결정되었다. 그리고 과학자들의 성취에는 인간네트워크, 기관의 지원, 생존경쟁과 운이 큰 영향을 미쳤고, 시대적 요구와 민족성이 발전의 토대가 되었다. 반면, 시각예술가들은 주변의 반대와 부적인 예술문화 풍토로 인해 고독한 삶을 살아왔다.

• Molecules and Cells
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• 제27권4호
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• pp.493-496
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• 2009

Hypoxia-inducible factor $1{\alpha}$ ($HIF1{\alpha}$) is a transcription factor that plays a key role in the adaptation of cells to low oxygen stress and oxygen homeostasis. The oxygen-dependent degradation (ODD) domain of $HIF1{\alpha}$ responsible for the negative regulation of $HIF1{\alpha}$ in normoxia is intrinsically unfolded. Here, we carried out the backbone $^1H$, $^{15}N$, and $^{13}C$ resonance assignment of a proteolysis-resistant fragment (residues 404-477) in the $HIF1{\alpha}$ ODD domain using NMR spectroscopy. About 98% (344/352) of all the $^1HN$, $^{15}N$, $^{13}C{\alpha}$, $^{13}C{\beta}$, and $^{13}CO$ resonances were unambiguously assigned. The results will be useful for further investigation of the structural and dynamic states of the $HIF1{\alpha}$ ODD domain and its interaction with binding partners.

• Child Health Nursing Research
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• 제10권2호
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• pp.165-172
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• 2004

The main purpose of this study is to examine the concepts appeared on research and provide future research directions in field of child health nursing. 205 studies of the total 318 studies had been analyzed first for the concepts of the client domain and this time 113 studies were analyzed for the practice, the client-nurse and the environmental domain in nursing. The practice domain includes mentalistitic, enactment, knowledge utilization, role related phenomenon, the client-nurse domain includes touch, communication, interaction phenomenon, and the environmental domain includes physical, social, symbolic environment. All were originally published between 1990 and 2000 in Korea. An analysis of concepts for this study was used the metaparadigm framework for nursing proposed by H. S. Kim(2000). 1. 103 studies belonged to the practice domain. Among them, 56(54％) studies used concepts related to enactment phenomenon like education(21.4％), giving information(7.1％), breast feeding(5.4％), caring(5.4％), airway suction(5.4％), nonnutritive sucking(5.4％). 44(43％) studies used concepts related to knowledge utilization like program development and evaluation of smoking, mother-infant interaction, home health nursing, obesity management. And only 3(3％) studies used role related concepts like quality of nursing, direction of health education, contents of child health nursing education. 2. Only 2(0.006％) studies belonged to the client-nurse domain. One concept is empathy in communication phenomenon and the other concept is role conflict in interaction phenomenon. 3. 8(0.02％) studies belonged to the environmental domain. Among them, 3 studies related to physical environment like space, noise and 5 studies related to social environment like social support, home environment. But the concept of symbolic environment was not used. The findings of this study provide the evidence that research related to the client-nurse domain and the environmental domain should be conducted actively to improve the practice of child health nursing. So that the research in field of child health nursing should be dealt with the concepts of four domains to develop knowledge systematically.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2008년도 제39회 하계학술대회
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• pp.625-626
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• 2008

The magnetic domain-refining techniques such as ball scratching, laser irradiation and plasma have been developed to reduce the domain wall spacing and thus iron losses in Fe-3%Si grain-oriented silicon steels. In view point of magnetic properties, it was supposed that the locally residual stresses change the magnetoelastic energy of the material and thus the spacing between $180^{\circ}$ domain walls decreases in order to reduce the magnetostatic energy. The effect of laser irradiation on iron loss and magnetostriction reduction for Fe-3%Si grain-oriented steel were investigated. Since the local tensile stresses were induced at the surface of Fe-3%Si steel by the laser irradiation, the minimum iron loss caused by reducing eddy current loss was obtained in spiete of the decrease of permeability by hindering eddy current loss was obtained in spite of the decrease of permeability by hindering the domain wall movement around the induced stress field. Furthermore, the laser treated 3%Si steel has lower magnetostriction as compared to non laser-treated steel and is less sensitive to applying pre-stresses due to the volume reduction of $90^{\circ}$ domain in materials.

• BMB Reports
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• 제42권8호
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• pp.506-510
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• 2009

The Toll signalling pathway in invertebrates is responsible for defense against Gram-positive bacteria and fungi, leading to the expression of antimicrobial peptides via NF-$\kappa$B-like transcription factors. Gram-negative binding protein 3 (GNBP3) detects beta-1,3-glucan, a fungal cell wall component, and activates a three step serine protease cascade for activation of the Toll signalling pathway. Here, we showed that the recombinant N-terminal domain of Tenebrio molitor GNBP3 bound to beta-1,3-glucan, but did not activate down-stream serine protease cascade in vitro. Reversely, the N-terminal domain blocked GNBP3-mediated serine protease cascade activation in vitro and also inhibited beta-1,3-glucan-mediated antimicrobial peptide induction in Tenebrio molitor larvae. These results suggest that the N-terminal GNBP homology domain of GNBP3 functions as a beta-1,3-glucan binding domain and the C-terminal domain of GNBP3 may be required for the recruitment of immediate down-stream serine protease zymogen during Toll signalling pathway activation.