• Title/Summary/Keyword: Research Diversity

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Bacterial communities in the feces of insectivorous bats in South Korea

  • Injung An;Byeori Kim;Sungbae Joo;Kihyun Kim;Taek-Woo Lee
    • Journal of Ecology and Environment
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    • v.48 no.2
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    • pp.120-127
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    • 2024
  • Bats serve as vectors and natural reservoir hosts for various infectious viruses, bacteria, and fungi. These pathogens have also been detected in bat feces and can cause severe illnesses in hosts, other animals, and humans. Because pathogens can easily spread into the environment through bat feces, determining the bacterial communities in bat guano is crucial to mitigate potential disease transmission and outbreaks. This study primarily aimed to examine bacterial communities in the feces of insectivorous bats living in South Korea. Fecal samples were collected after capturing 84 individuals of four different bat species in two regions of South Korea, and the bacterial microbiota was assessed through next generation sequencing of the 16S rRNA gene. The results revealed that, with respect to the relative abundance at the phylum level, Myotis bombinus was dominated by Firmicutes (47.24%) and Proteobacteria (42.66%) whereas Miniopterus fuliginosus (82.78%), Rhinolophus ferrumequinum (63.46%), and Myotis macrodactylus (78.04%) were dominated by Proteobacteria. Alpha diversity analysis showed no difference in abundance between species and a significant difference (p < 0.05) between M. bombinus and M. fuliginosus. Beta-diversity analysis revealed that Clostridium, Asaia, and Enterobacteriaceae_g were clustered as major factors at the genus level using principal component analysis. Additionally, linear discriminant analysis effect size was conducted based on relative expression information to select bacterial markers for each bat species. Clostridium was relatively abundant in M. bombinus, whereas Mycoplasma_g10 was relatively abundant in R. ferrumequinum. Our results provide an overview of bat guano microbiota diversity and the significance of pathogenic taxa for humans and the environment, highlighting a better understanding of preventing emerging diseases. We anticipate that this research will yield bioinformatic data to advance our knowledge of overall microbial genetic diversity and clustering characteristics in insectivorous bat feces in South Korea.

A Study on the Construction of Database, Online Management System, and Analysis Instrument for Biological Diversity Data (생물다양성 자료의 데이터베이스화와 온라인 관리시스템 및 분석도구 구축에 관한 연구)

  • Bec Kee-Yul;Jung Jong-Chul;Park Seon-Joo;Lee Jong-Wook
    • Journal of Environmental Science International
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    • v.14 no.12
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    • pp.1119-1127
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    • 2005
  • The management of data on biological diversity is presently complex and confusing. This study was initiated to construct a database so that such data could be stored in a data management, and analysis instrument to correct the problems inherent in the current incoherent storage methods. MySQL was used in DBMS(DataBase Management System), and the program was basically produced using Java technology Also, the program was developed so people could adapt to the requirements that are changing every minute. We hope this was accomplished by modifying easily and quickly the advanced programming technology and patterns. To this end, an effective and flexible database schema was devised to store and analyze diversity databases. Even users with no knowledge of databases should be able to access this management instrument and easily manage the database through the World Wide Web. On a basis of databases stored in this manner, it could become routinely used for various databases using this analysis instrument supplied on the World Wide Web. Supplying the derived results by using a simple table and making results visible using simple charts, researchers could easily adapt these methods to various data analyses. As the diversity data was stored in a database, not in a general file, this study makes the precise, error-free and high -quality storage in a consistent manner. The methods proposed here should also minimize the errors that might appear in each data search, data movement, or data conversion by supplying management instrumentation on the Web. Also, this study was to deduce the various results to the level we required and execute the comparative analysis without the lengthy time necessary to supply the analytical instrument with similar results as provided by various other methods of analysis. The results of this research may be summerized as follows: 1)This study suggests methods of storage by giving consistency to diversity data. 2)This study prepared a suggested foundation for comparative analysis of various data. 3)It may suggest further research, which could lead to more and better standardization of diversity data and to better methods for predicting changes in species diversity.

Evaluating genetic diversity and identifying priority conservation for seven Tibetan pig populations in China based on the mtDNA D-loop

  • Ge, Qianyun;Gao, Caixia;Cai, Yuan;Jiao, Ting;Quan, Jinqiang;Guo, Yongbo;Zheng, Wangshan;Zhao, Shengguo
    • Asian-Australasian Journal of Animal Sciences
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    • v.33 no.12
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    • pp.1905-1911
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    • 2020
  • Objective: Tibetan pigs, an excellent species unique to China, face serious threats, which in turn affects the development and utilization of the outstanding advantages of plateau hypoxia adaptability and reduces their genetic diversity. Therefore, a discussion of measures to conserve this genetic resource is necessary. The method, based on genetic diversity, genetic divergence and total genetic contribution rate of population, reflects the priority conservation order and varies depending on the three different purposes of conservation. Methods: We analyzed mitochondrial DNA control region (D-loop) variation in 1,201 individuals from nine Tibetan pig populations across five provinces and downloaded 564 mtDNA D-loop sequences from three indigenous pig breeds in Qinghai, Sichuan, and Yunnan Provinces distributed near the Tibetan pigs. Results: We analyzed three different aspects: Changdu Tibetan pigs have the highest genetic diversity, and from the perspective of genetic diversity, the priority conservation is Changdu Tibetan pigs. Hezuo Tibetan pigs have the highest genetic contribution, so the priority conservation is Hezuo Tibetan pigs in the genetic contribution aspect. Rkaze Tibetan pigs were severely affected by indigenous pig breeds, so if considering from the perspective of introgression, the priority conservation is Rkaze Tibetan pigs. Conclusion: This study evaluated genetic diversity and comprehensively assessed conservation priority from three different aspects in nine Tibetan pig populations.

Genetic Diversity and Phylogenetic Relationship of Genus Phyllostachys by RAPD Markers (RAPD분자 마커를 이용한 왕대속 대나무의 유전적 다양성 및 계통 관계)

  • Lee, Song-Jin;Huh, Man-Kyu;Shin, Hyun-Cheol;Huh, Hong-Wook
    • Journal of Life Science
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    • v.20 no.6
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    • pp.819-824
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    • 2010
  • Genus Phyllostachys is a long-lived woody species primarily distributed throughout South East Asia. Many species of this genus has been regarded as medically and ecologically important in the world. We evaluated representative samples of the four taxa with RAPD to estimate genetic relationships within the genus Phyllostachys. The percentages of polymorphic loci were 8.9-33.3% at the species level. P. bambusoides was found to show lower genetic diversity (H=0.018) than other species. Total genetic diversity ($H_T$) was 0.315, genetic diversity within populations ($H_S$) was 0.043, the proportion of total genetic diversity partitioned among populations ($G_{ST}$) was 0.659 and the gene flow (Nm) was 0.0263. As some Korean populations were isolated and patchily distributed, they exhibited low levels of genetic diversity. The four taxa of the genus Phyllostachys analyzed were distinctly related to a monophyletic. P. nigra var. henonis. Stapf was found to be more closely related to P. pubescens than to P. nigra. P. bambusoides was quite distinct from the remaining species.

Successional changes in plant composition over 15 years in a created wetland in South Korea

  • Son, Deokjoo;Lee, Hyohyemi;Cho, Kang-Hyun;Bang, Jeong Hwan;Kwon, Oh-Byung;Lee, Eun Ju
    • Journal of Ecology and Environment
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    • v.42 no.4
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    • pp.183-190
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    • 2018
  • Backgrounds: The main purpose of this research was to assess changes in vegetation structure, wetland index, and diversity index for a 15-year-old created wetland in Jincheon, South Korea. The created wetland consists of four sub-wetlands: a kidney-shaped wetland, a ditch, an ecological pond, and a square wetland. Vegetation and water depth data were collected at each site in 1999 and 2013, and Shannon diversity and wetland indices were calculated. Results: The total number of plant species increased from 18 in 1999 to 50 in 2013, and the ecological pond in 1999 and the ditch in 2013 presented the highest diversity indices (2.5 and 3.2, respectively). Plant species were less diverse in 1999 than in 2013, presumably because these initial wetlands were managed periodically for water purification and installation of test beds. The proportion of wetland plants, including obligate wetland and facultative wetland species, decreased from 83 to 56%, whereas upland plants, including obligate upland and facultative upland species, increased from 17 to 44%. After ceasing water supply, water depth in all four sub-wetlands declined in 2013. Thus, upland plants established more readily at these sites, resulting in higher diversity and lower wetland indices than in 1999. Conclusions: The major floristic differences between 1999 and 2013 were an increase in the number of upland plants and a decrease in wetland species. Although wetland indices were lower in 2013, the created wetland performed important ecosystem functions by providing habitats for wetland and upland plants, and the overall species diversity was high.

Seasonal Variation of Bacterial Community Composition in Sediments and Overlying Waters of the South East Sea (동해 남부 해역 퇴적물과 저층 해수 세균 군집 조성의 계절적 변화 연구)

  • Choi, Dong Han;Gim, Byeong-Mo;Choi, Tae Seob;Lee, Jung-Suk;Noh, Jae Hoon;Park, Young-Gyu;Kang, Seong-Gil
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.19 no.2
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    • pp.147-154
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    • 2014
  • Bacteria play an important role in biogeochemical cycles in marine environments and their functional attributes in ecosystems depend primarily on species composition. In this study, seasonal variation of bacterial diversity was investigated by pyrosequencing of 16S rDNA in surface sediment and overlying seawater collected in the south East Sea, planned for the site of $CO_2$ sequestration by the carbon capture and storage (CCS) project. Gammaproteobacteria was dominant in the sediment in most seasons, whereas Alphaproteobacteria was a most dominant group in the overlying water. Thus, the bacterial diversity greatly differ between sediment and seawater samples. On the genus level, bacterial diversity between two habitats was also different. However, the number of genera found over 5% were less than 10 in both habitats and the bacterial community was composed of a number of diverse minor or rare genera. Elevation of $CO_2$ concentration during a $CO_2$ storage process, could result in change of bacterial diversity. Thus, this study will be very useful to access the effect of $CO_2$ on bacterial diversity and to predict functional change of the ecosystem during the process of CCS project.

The Effect of Team Characteristics of Technology-based Startup Programs on Patent Performance: Focusing on Team Diversity (기술기반 창업 프로그램의 팀 특성이 특허 성과에 미치는 효과 분석: 팀 다양성을 중심으로)

  • Lee, Jai Ho;Sohn, Youngwoo;Han, Jung Wha;Lee, Sang-Myung
    • Knowledge Management Research
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    • v.25 no.1
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    • pp.21-41
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    • 2024
  • The global Industry has been shaped by start-ups that originated with knowledge-based innovative strategies or technologies in the 21st century. Specifically, laboratory start-ups that rely on research papers or patents for new technology development are recognized for their high survival rate and the creation of employment opportunities. Our study concentrated on 'I-Corps', which also introduced in Korea, standing for innovation corps is a laboratory startup program launched in 2011 by the NSF(National Research Foundation) to commercialize R&D results and foster entrepreneurship as part of the policy to build a start-up system at the national innovation level. In this study, we proposed and empirically tested a research model focusing on teams participating in the I-Corps program to determine how startup team diversity, among the team characteristics of laboratory startups, affected patent performance. As a result of the analysis, among the proposed variables, age diversity, educational background diversity, and value diversity had a significant impact on patent performance. The results of this study are expected to further strengthen the theoretical and practical foundations of researchers or practitioners of the I-Corps program, as well as related areas involving technology & laboratory startups, intellectual property and knowledge management fields in the future.

Studies on β-diversity for high plant community turnover in flood plain restoration (수변 복원 시 식물종 다양성 증진을 위한 β-diversity 연구)

  • Han, Young-Sub;Kim, Hae-Ran;Han, Seung-Ju;Jeong, Jung-Kyu;Lee, Seung-Hyuk;Jang, Rae-Ha;Cho, Kyu-Tae;Kang, Tay-Gyoon;You, Young-Han
    • Journal of Wetlands Research
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    • v.15 no.4
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    • pp.501-508
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    • 2013
  • We have researched heterogeneity of naturalized river plant community by ${\beta}$-diversity for restoration of river community which has high diversity plant species. As a result the average of heterogeneity was 0.32(range 0.23~0.37) from the river to the inland. This value shows community turnover of species composition of plant communities 6 times. The ${\beta}$-diversity was no difference among water system of Seomjin river, Han river, Nakdong river and Geum river. The upper-river valley(0.36) was higher than lower-river valley(0.23) in each water system(p level<0.05). Multiple regressing analysis was used for look the relationship with Environmental factors as a result, it shows ${\beta}$-diversity significant on a slope. River mimetic diagram with dominant species that appear through Belt-transect painted. Dominant plant species turned 6 time in upper-river and turned about 5 time in lower-river. The result of this study suggested practical basis of planting species and planting pattern. To improve species diversity of river plant community, slope degree raise is the most important.