• Clinical and Experimental Reproductive Medicine
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• 제30권1호
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• pp.105-109
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• 2003

Objective s: To assess the fertilizing capacity using sperm penetration assay (SPA) to predict the outcome of the in vitro fertilization-embryo transfer (IVF-ET) outcome. Materials and Methods: Semen samples were provided by 129 patients undergoing IVF. We attempted to correlate the extent of sperm penetration under enhanced SPA protocol with the results of fertilization, cleavage, preimplantation embryo development, and pregnancy. Results: Univariate analysis demonstrated a statistically significant correlation between fertilizing capacity and motility, kinetics, fertilization, cleavage and embryo development, and pregnancy rate. By logistic regression analysis, fertilizing capacity was found to be the only variable that was statistically significant with respect to pregnancy rate. Fertilizing capacity, cleavage rate and pregnant rate were significantly higher in pregnant group. However, the fertilization rates was comparable with both group. Conclusions: Lower fertilizing capacity could denote a poorer prognosis for establishing a pregnancy, even after satisfactory fertilization rate is achieved.

• Asian-Australasian Journal of Animal Sciences
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• 제31권12호
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• pp.1980-1990
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• 2018

Objective: The aim of this study was to discover the functional impact of non-synonymous single nucleotide polymorphisms (nsSNPs) that were found in selective sweep regions of the Landrace genome Methods: Whole-genome re-sequencing data were obtained from 40 pigs, including 14 Landrace, 16 Yorkshire, and 10 wild boars, which were generated with the Illumina HiSeq 2000 platform. The nsSNPs in the selective sweep regions of the Landrace genome were identified, and the impacts of these variations on protein function were predicted to reveal their potential association with traits of the Landrace breed, such as reproductive capacity. Results: Total of 53,998 nsSNPs in the mapped regions of pigs were identified, and among them, 345 nsSNPs were found in the selective sweep regions of the Landrace genome which were reported previously. The genes featuring these nsSNPs fell into various functional categories, such as reproductive capacity or growth and development during the perinatal period. The impacts of amino acid sequence changes by nsSNPs on protein function were predicted using two in silico SNP prediction algorithms, i.e., sorting intolerant from tolerant and polymorphism phenotyping v2, to reveal their potential roles in biological processes that might be associated with the reproductive capacity of the Landrace breed. Conclusion: The findings elucidated the domestication history of the Landrace breed and illustrated how Landrace domestication led to patterns of genetic variation related to superior reproductive capacity. Our novel findings will help understand the process of Landrace domestication at the genome level and provide SNPs that are informative for breeding.

• Clinical and Experimental Reproductive Medicine
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• 제25권1호
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• pp.1-8
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• 1998

항정자항체의 종류 및 존재부위가 정액성상 및 수정능력에 미치는 영향을 조사하였다. 항정자항체의 종류 및 존재부위는 immunobead binding test에 의하여 시행하였으며, 정자와 수정능력은 투명대제거 햄스터 난자 침입법에 의하여 시행하였다. 항정자항체는 정자수, 운동성 및 운동지수에 악영향을 끼쳤으며, 수정능력에도 악영향을 끼쳤다. 항정자항체의 존재부위에 따른 차이는 보이지 않았다. 항정자항체 IgG가 정자두부 혹은 정자미부에 존재할 경우 및 항정자항체 IgA가 정자미부에 존재할 경우 수정능력을 크게 감소시켰다.

• 한국발생생물학회지:발생과생식
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• 제23권2호
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• pp.101-110
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• 2019

Melatonin is a pineal hormone that is synthesized and released at night under the light and dark cycles of a day. Its effects on the reproductive activities have well been established by the administration through various routes in photoperiodic animals. It was also identified in plants and named phytomelatonin. The capacity of the phytomelatonin was investigated in this investigation whether it affects the reproductive function in male golden hamster. As expected, animals housed in long photoperiod (long photoperiod, LP>12.5 hours of lights in a day) had large testes and animals kept in short photoperiod ($$SP{\leq_-}12.5$$ hours of lights in a day) showed remarkably reduced testes. The dietary supplement with melatonin itself induced the complete involution of testes. Pistachios that were reported to contain a large amount of melatonin demonstrated no effects at all in male golden hamsters. These results suggest that dietary supplement containing melatonin-rich foodstuff used in this investigation may not be enough to affect the reproductive endocrine system in male golden hamsters.

• Clinical and Experimental Reproductive Medicine
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• 제13권1호
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• pp.29-38
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• 1986

In order to study the mechanism of follicular atresia, follicles were classified into the normal groups and the atretic ones, according to the criteria with or without corpus luteum, size of follicles, vascularization, status of granulose cells and the hypertrophy of theca layers in the porcine ovary. To estimate the binding capacity of human chorionic gonadotropin (hCG) receptor on the granulosa cells during atresia, hCG were iodinated by the chloramine-T method and then purified through the column chromatography. The concentration" of hCG receptor in each group were measured by the hCG receptor binding assay. Binding capacity in large normal follicles were 1.16%, but atretic ones were 0.45%. But in medium and small follicles (below 6mm in diameter), the binding capacity in normal follicles were 0.09%, but atretic ones were 0.05%, which was lower than those of large follicles. The present ( ) that the concentrations of hCG receptors on granulosa cells is decreased when the follicles become atretic and be used as a sort of creteria for the identification of follicles atresia.

• 한국산림과학회지
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• 제21권1호
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• pp.35-38
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• 1974

싸리류(類)에 대(對)하여 매년(每年) 베어서 이용(利用) 하였을 때 재생능력(再生能力)을 조사(調査)검토 하고저 싸리 조록싸리, 풀싸리, 늦싸리, 참싸리 종자(種子)를 1969년(年) 봄 $m^2$당 4g씩 난괴법에 의하여 6반복 파종하고 당년 10월말(月末) 성적을 조사(調査)한 후 지상부(地上部)를 베어 주고 1970년(年) 봄에 $m^2$당 6주(株)씩 난괴법에 의하여 6반복 식재(植栽)하였다. 1970년(年)부터 1972년(年)까지 10월말(月末) 건장(乾長) 근원경(根元徑) 및 맹아본수(萌芽本數)를 조사(調査)하고 매년(每年) 11월(月) 지상부(地上部)를 베어 주고 근주(根株)만 남겨 놓았다. 1. 건장(乾長) 근원경(根元徑)의 재생능력(再生能力)은 품종별(品種別) 주령별(株齡別) 1%가 넘는 고도(高度)의 유의성(有意性)이 있었으며 싸리, 풀싸리, 참싸리, 조록싸리, 늦싸리의 순위로 좋았다. 2. 맹아본수(萌芽本數)의 재생력(再生力)은 조록싸리, 늦싸리, 풀싸리, 싸리, 참싸리의 순위로 나타났으며 특(特)히 참싸리가 저조한 맹아력(萌芽力)을 나타내고 있음은 건장(乾長) 근원경(根元徑)의 순위(順位)와 차이(差異)가 있었다. 3. 싸리류(類)는 주령(株齡) 4년(年)까지는 매년(每年)베어서 이용(利用) 하드라도 퇴화하지 않고 재생력(再生力)을 가지고 주령(株齡)이 증가(增加)함에 따라 건장(乾長) 근원경(根元徑) 주당(株當) 맹아(萌芽) 본수(本數)가 증가(增加)하였다.

• 한국산림과학회지
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• 제35권1호
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• pp.15-18
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• 1977

싸리류(類)에 대(對)하여 매년(每年) 베어서 이용(利用)하였을 때 재생능력(再生能力)을 조사(調査)하고저 싸리, 조록싸리, 풀싸리, 늦싸리, 참싸리 종자(種子)를 1969년(年) 봄 $m^2$당(當) 4g씩 난괴법에 의하여 6반복 파종(播種)하고 당년(當年) 10월말(月末) 성적(成績)을 조사(調査)한 후(後) 지상부(地上部)를 베어주고 1970년 봄에 $m^2$당(當) 6주(株)씩 난괴법에 의(依)하여 6반복(反復) 식재(植栽)하였다. 1970년(年)부터 1976년(年)까지 10월말(月末) 간장(幹長) 근원경(根元徑) 및 맹아본수(萌芽本數)를 조사(調査)하고 매년(每年) 11월(月) 지상부(地上部)를 베어주고 근주(根株)만 남겨 놓았다. 1. 간장(幹長) 주당맹아본수(株當萌芽本數)의 재생능력(再生能力)은 품종별(品種別) 주기별(株畿別) 통계적(統計的) 유의성(有意性)을 인정(認定)할 수 있었으며 간장(幹長)의 재생능력(再生能力)은 싸리가 주당맹아본수(株當萌芽本數)은 조록싸리가 가장 좋았다. 2. 싸리류(類)를 매년(每年) 벌채(伐採)하였을 때 간장(幹長) 주당맹아본수(株當萌芽本數)의 재생능력(再生能力)의 극대치(極大値)는 주령(株齡) 5년(年)이었다.

• Animal Bioscience
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• 제36권11호
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• pp.1619-1631
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• 2023

The objective of this review was to provide an overview of the effects of dietary fiber (DF) on reproductive performance in gestating sows. Dietary fibers have been suggested to modulate microbiota in the intestine and the immune system of gestating sows and to improve gut health. Thus, DF may help alleviate the adverse effects of the stressful production cycle of gestating sows. These benefits may subsequently result in improved reproductive performance of sows. Previous studies have reported changes in microbiota by providing gestating sows with DF, and the responses of microbiota varied depending on the source of DF. The responses by providing DF to gestating sows were inconsistent for antioxidative capacity, hormonal response, and inflammatory response among the studies. The effects of DF on reproductive performance were also inconsistent among the previous studies. Potential reasons contributing to these inconsistent results would include variability in reproductive performance data, insufficient replication, influence of other nutrients contained in the DF diets, characteristics of DF, and experimental periods. The present meta-analysis suggests that increasing the total DF concentration by 10 percentage units (e.g., 12% to 22% as-fed basis) in gestating sow diets compared to the control group improves the litter born alive by 0.49 pigs per litter. However, based on the present review, questions remain regarding the benefits of fibers in gestating sow diets. Further research is warranted to clarify the mode of action of fibers and the association with subsequent reproductive performance in gestating sows.

• Clinical and Experimental Reproductive Medicine
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• 제23권1호
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• pp.81-86
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• 1996

The assessment of acrosomal status is important in evaluating the ability of sperm to fertilize the egg. The acrosomal status of sperm from 47 normal volunteers with proven fertility and 167 subfertile men with not to achieve pregnancy for at least 1 year were evaluated with Acrobeads test(FUSO Pharmaceutical Industries, Ltd, Japan) using immunobeads coated with MH61 monoclonal antibody, which is specific for acrosome-reacted sperm. The mean${\pm}$SD of acrobeads score in 47 volunteer group was $2.8{\pm}0.7$, of which 46(97.9%)cases were ${\geq}$ 2. The mean${\pm}$SD of acrobeads score in 167 subfertile group was $1.7{\pm}0.8$, of which 73(79.3%)cases were ${\leq}$ 1. The aerobe ads score in subfertile group were significantly lower(r=0.294, p<0.05) than those in volunteer group. In subfertile group, acrobeads score were well correlated with the sperm density and motility(r=0.275, r=0.281, p<0.01), but not with semen volume(r=0.16) and serum hormone level(FSH r=0.084, LH r=0.036, testosterone r=0.058, prolactin r=0.006 and estradiol r=0.060)(p>0.05). Of 63 subfertile cases with normozoospermia, 22(34.9%)cases showed 0 or 1 of acrobeads score, which means to accompany with a functional defect in spite of normal morphology. As a results, Acrobeads test is not only a technically simple sensitive procedure with good reproducibility in evaluating the sperm fertilizing capacity but also an useful in the evaluation of effectiveness in the treatment of infertility and the separation of acrosome-reacted sperm in the assisted reproductive technique.

• Asian-Australasian Journal of Animal Sciences
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• 제24권9호
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• pp.1199-1203
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• 2011

The objective of this study was to examine the binding potential of sperm to the epithelium of the sperm storage tubules (SST) in vitro and in vivo to assess the functional maturation of fowl sperm. Sperm from the testis, epididymis, as well as the proximal, middle and distal vas deferens were incubated in vitro with either the uterovaginal junction (UVJ)- or infundibular tissue containing SST at $39^{\circ}C$ for 30 min. Aliquots of sperm were also artificially inseminated into the uteri of hens, and the UVJ and infundibulum were collected 24 h post artificial insemination (AI). After incubation and AI, tissues were washed to remove loosely adhered sperm and subjected to fluorescence staining with 4', 6-diamidino-2-phenylindole, dihydrochloride (DAPI) for counting the number of bound sperm per 0.25 mm2 of surface area. Sperm from the testis, epididymis, and the three segments of the vas deferens exhibited their differential (p<0.05) binding capacity, which increased gradually from the testicular to distal vas deferens sperm under both in vitro and in vivo conditions. Existing similar trend, sperm, regardless of their source had a lesser affinity to bind to the epithelium of the infundibular SST than to the UVJ-SST. These experimental results suggested that fowl sperm may undergo gradual changes in the process of functional maturation, whereby they gain the ability to bind to the epithelium of the SST during their passage through the male reproductive tract (MRT).