• BMB Reports
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• 제33권2호
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• pp.155-161
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• 2000

The saccharomyces cerevisiae Rad27, a structure-specific endonuclease for the okazaski fragment maturation has been known to interact genetically and biochemically with Dna2, an essential enzyme for DNA replication. In an attempt to define the significance of the interaction between the two enzymes, we expressed and purified both Dna2 and Rad27 proteins. In this report, Rad27 could not form a complex with Dna2 in the three different analyses. The analyses included glycerol gradient sedimentation, protein-column chromatography, and coinfection of baculoviruses followed by affinity purification. This is in striking contrast to the previous results that used crude extracts. These results suggest that the interaction between the two proteins is not sufficiently stable or indirect, and thus requires an additional protein(s) in order for Rad27 and Dna2 to form a stable physical complex. This result is consistent with our genetic findings that Schizosaccharomyces pombe Dna2 is capable of interacting with several proteins that include two subunits of polymerase $\delta$, DNA ligase I, as well as Fen-1. In addition, we found that the N-terminal modification of Rad27 abolished its enzymatic activity. Thus, as suspected, we found that on the basis of the structure determination, N-terminal methionine indeed plays an important role in the nucleolytic cleavage reaction.

• 대한장애인치과학회지
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• 제4권2호
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• pp.77-81
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• 2008

골화석증(Osteopetrosis)은 전신의 뼈가 대리석 모양으로 골 경화를 일으키며 골수강의 폐쇄 및 골의 취약성을 보이는 드문 질환이다. 성인형(Adult form)은 우성 유전되며 경미한 증상을 나타낸다. 반면에 유아형(Infantile form)은 열성 유전되면서 심각한 증상을 나타내고, 조기에 사망에 이를 수도 있다. 골화석증은 골격계, 조혈계, 신경계에서 다양한 증상을 유발한다. 본 증례의 목적은 드물게 발생하는 유아형 골화석증을 가진 2 명의 환아를 치료한 후, 그 질환과 치과적 관리에 대해 고찰하고자 함이다. 첫 번째 증례는 골화석증으로 진단된 5세 8개월 남환이었다. 임상 및 방사선 검진 결과 악골 및 치아의 변형을 나타내었으나, 특별한 병적 소견이 없어 예방 치료 및 주기적인 검진 시행하였다. 두 번째 증례는 중증도의 치아우식증을 가진 환아로 의료진과의 협조 하에 수혈한 후 발치하였다. 유아형 골화석증은 다양한 구강 합병증을 유발하며, 질환의 심각성에 의해 치료 자체도 어렵다. 치과의사로서 골화석증 환아를 관리할 때에는 건전한 구강 상태를 유지하기 위한 지속적인 관심과 예방 치료가 필요하며, 아무리 간단한 발치나 치과 술식이더라도 의료진과 협조를 해야 한다.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2008년도 춘계학술대회 논문집 전기설비전문위원
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• pp.25-27
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• 2008

This paper discusses the implications of the MASTER SPEC project and its introduction to Korea in terms of social and political contexts. The motives for this report starts from both theoretical and empirical puzzles. Its theoretical motive is concerned to the right form of governance of electric-construction standardardization. Upon the experience of the United States and many European countries, we have acknowledged the role of government in the setting of industrial policy formats and standardization. However, the mechanisms of industrial governance have greatly changed these days, from the centralized one to the participatory one. We call the latter type governance. The form of governance implies the participation of both public and private actors in the policy-making of industrial matters. We have sought to find a better format in the case of electric-construction field. The answer is: the governance initiated by the private actors rather than the government-centered one. The project of MASTER SPEC, in this sense, has many probable implications toward the standard-setting of future industrial managements in Korea.

• International Journal of Safety
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• 제6권2호
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• pp.17-21
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• 2007

This paper aims at the statistical analysis of electrical fire and classification of electrical fire causes to collect electrical fires data efficiently. Electrical fire statistics are produced to monitor the number and characteristics of fires attended by fire fighters, including the causes and effects of fire so that action can be taken to reduce the human and financial cost of fire. Electrical fires make up the majority of fires in Korea(including nearly 30% of total fires according to recent figures), The incorrect and biased knowledge for electrical fires changed the classification of certain types of fires, from non-electrical to electrical. It is convenient and required to develop the standardized form that makes, in the assessment of the cause of electrical fires, the fire fighters directly ticking the appropriate box on the fire report form or making an assessment of a text description. Therefore, it is highly recommended to develop electrical fire cause classification and electrical fire assessment on the fire statistics in order to categorize and assess electrical fires exactly. In this paper newly developed electrical fire cause classification structure, which is well-defined hierarchical structure so that there are not any relationship or overlap between cause categories, is suggested. Also fire statistics systems of foreign countries are introduced and compared.

• 대한조선학회논문집
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• 제42권1호
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• pp.50-56
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• 2005

The performance of Traditional Korean Fishing Boat is surveyed based on Report on Investigation of Korean Fishing Boat. The characteristics of Traditional Korean fishing Boat of hull form, rudder, sail are studied from the view point of modern sailing boat design. As a result we find her hull form is an excellent candidate of mother ship for modern boat design and the great sized rudder of Traditional Korean Fishing Boat makes a role of the centerboard of modern sailing host. And also we find the sail of Traditional Korean fishing Boat is more useful for sailing against the wind direction than an ordinary Lug sail.

• 대한골관절종양학회지
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• 제7권2호
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• pp.64-67
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• 2001

유상피육종은 전형적인 형태인 경우에는 비교적 젊은 사람의 손이나 발과 같은 말단부에 호발하는 종양이다. 그러나 근위형의 경우는 주로 청장년층의 골반, 회음부 또는 생식기에 발생한다. 형태학적으로 종양세포는 뚜렷한 유상피 형태를 취하거나, rhabdoid형태를 보이며, 심한 세포학적 이형증과 함께, 다결절 형태로 성장한다. 면역조직화학적 검사에서는 특징적으로 cytokeratin, epithelial membrane antigen, vimentin이 양성이다. 본 보고는 32세 남자의 회음부에 발생한 근위형 유상피육종을 기술하였다. 형태학적으로 전형적인 근위형 유상피 육종의 조직학적 소견을 관찰하였으며, 병리조직학적 감별점과, 환자의 예후에 관하여 토론하였다.

• 대한한방내과학회지
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• 제41권2호
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• pp.150-158
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• 2020

Objectives: This study aimed to investigate the effect of Soeumin-Seonghyangjeongki-san on diarrhea with tube feeding causing mental deterioration induced by subdural hemorrhage (SDH) in an 83-year-old male patient. Methods: The patient was treated with Soeumin-Seonghyangjeongki-san. His symptoms were assessed using the Bristol stool form index, the King's Stool Chart, and records of stool state. The treatment was executed for one week from September 10, 2018 to September 16, 2018. The patient was observed during that same period. Results: After being treated with Soeumin-Seonghyangjeongki-san for seven days, the patient's diarrhea symptoms were evaluated using the Bristol stool form index, the King's Stool Chart, and records of stool state. After the treatment, the results of all three evaluation methods showed that the patient's condition had improved. Conclusions: The results suggest that Soeumin-Seonghyangjeongki-san can be a valuable option for treating diarrhea with tube feeding causing mental deterioration induced by SDH.

• 사상체질의학회지
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• 제21권3호
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• pp.107-121
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• 2009

1. Objectives: We aimed to know what are different in physiological and pathological symptoms associated with health state according to Sasang Constitution in women 2. Methods: We recruited 338 women between 10 and 80, excluding individuals who have physically or psychologically serious disease, or women in pregnancy among Korean population in Traditional Korea Clinics. We used CRF(Case Report Form, C-2009-002439) as measurement of physiological and pathological symptoms and SF-36 (Short Form-36) as measurement of health state We analyzed association between physiological and pathological symptoms and health state with Two-way ANOVA 3. Results: There are 9 different symptoms associated with health state, such as digestion state, uncomfortable problem in belly, indigestion in bad condition, abnormal sweat, night sweat, clean in urine, no problem in urine, feel heavy in chest according to Sasang constitution. 4. Conclusions: Physiological and pathological symptoms are associated with health state and some symptoms associated with health state are different according to Sasang Constitution in women.

• BMB Reports
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• 제41권2호
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• pp.132-138
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• 2008

AtbZIP16 and AtbZIP68 are two putative G group bZIP transcription factors in Arabidopsis thaliana, the other three members of G group bZIPs are GBF1-3 which can bind G-box. Members of G group have conservative protein structure: highly homological basic region and a proline-rich domain in the N-terminal region. Here, we report that AtbZIP16 and AtbZIP68 could bind cis elements with ACGT core, such as G-box, Hex, C-box and As-1, but with different binding affinities which from high to low were G-box > Hex > C-box > As-1; AtbZIP16 and AtbZIP68 could form homodimer and form heterodimer with other members of G group; N-terminal proline rich domain of AtbZIP16 had transactivation activity in yeast cells while that of AtbZIP68 did not; AtbZIP16 and AtbZIP68 GFP fusion protein localized in the nucleus of onion epidermal cells. These results indicated that AtbZIP16 and AtbZIP68 were two new members of GBFs. In Arabidopsis, AtbZIP16 and AtbZIP68 may also participate in light-responsive process in which GBF1-3 are involved.

• Neonatal Medicine
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• 제18권1호
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• pp.143-147
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• 2011

ASAuria는 요소회로이상증 중의 하나로 드물게 나타나는 상염색체 열성으로 유전되는 대사 질환이다. 체내에 ASA가 축적됨으로서 신생아 시기에 구토, 기면, 수유 곤란, 의식 장애를 보이며 적절한 조치를 하지 않으면 사망에 이르게 되는 치명적인 대사 질환이다. 이 질환은 혈중과 소변에 ASA가 증가하는 것으로 진단할 수 있다. 국내에서는 고전적 형태의 ASAuria가 아직 보고된 사례는 없으며, 이에 본 저자들은 고전적 ASAuria로 진단된 환아에서 유전자 검사를 통해 보인자 부모로부터 출생하였음을 진단한 신생아 환자 1예를 경험하였기에 보고하는 바이다.