• Journal of Preventive Medicine and Public Health
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• 제23권3호
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• pp.285-295
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• 1990

The utilization of medical care services has been partly regionalized with the implementation of referral requirement by the government since July 1, 1989 when the health insurance coverage was extended to all the people. For the purpose of regionalization, the whole country has been primarily divided into tertiary care regions, and each of them again into secondary care regions. This study investigates the self-sufficiency for in-patient care services of secondary care regions focusing on why it varies among the regions. In doing so, analysis is performed to examine a model which embodies three sets of hypotheses as follows : 1) The regional self-sufficiency for medical care services would be subject to direct influences of regional characteristics, amount of available services and structural properties of regional medical care system ; 2) The regional characteristics would have indirect effects on the self-sufficiency which are mediated by medical care services ; and 3) The amount of available services would indirectly affect the self-sufficiency by influencing the structure of regional medical care system. The results of analysis were generally consistent with the model. The findings have some practical implications. The regional self-sufficiency for medical care services partly depends upon basic properties of each region which cannot be changed in a short period of time. Thus the self-sufficiency for medical care services can be improved mainly by health policy measures. In some of the regions the self-sufficiency for in-patient care services was much higher or lower than can be predicted from the bed-population ratio. Indication is that the allocation of health resources should be made considering a variety of factors bearing upon the supply of and demand for health care ; not on the basis of just a single criterion like the availability. The self-sufficiency of a certain region is related to not only its own characterstics but also the characteristics of neighboring regions. Therefore, attention should be also directed to the inter-regional relationships in health care when the needs for investment of health resources in a region are assessed. However, it should be noted that this study used the data collected before the referral requirement was imposed. A replication of this analysis using recent data would provide an evaluation of the impact on the self-sufficiency of the referral requirement as well as a confirmation of the findings of this study.

• 한국가금학회지
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• 제27권1호
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• pp.25-30
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• 2000

Present study was conducted to investigate effects of microbial phytase in laying hen diets on utilization of non-phytate phosphorus(NPP) whose levels were adjusted to be adequate or lower than that of NRC requirements. Birds of control roup were fed a diet containing 0.275% NPP and 3.4% Ca, satisfying the NRC(1994) feeding standard. bird on T1, T2 and T3 were allowed to eat diets containing NPP at 100, 80 and 60%, respectively, of Control group, and 4.0% Ca level along with a microbial phytase added at a level of 300 DPU. Three hundred and sixty, ISA Brown layers, 23-week-old, divided into four treatment groups with three replications per treatment and 30 layers per replication were fed the diets for 12 weeks. Levels of feed intake were not different among the groups, The egg mass/feed intake ratio appeared better in T2 group by about 8%, though without a statistical significance, compared to that of control. Egg production fate tended to be improved over the control group by feeding the 100%(T1) and 80%(T2) NPP diets added with phytase, with a significant difference for T2(p<0.05). Mean egg weight and egg shell quality, measured by breaking strength and thickness of the egg shell, of the T2 group tended to show numerically better, without a significance than those of control. Furthermore, birds of the T2 group showed higher calcium and phosphorus contents in tibia by about 9%(p<0.05) than the control. Overall performances of birds in T1 appeared better than those of control, but tended to be lower than those of the T2. The birds in T3 performed similar to the those of the other dietary groups except the relatively low tibia calcium level. In conclusion, the results of this study suggest that supplementation of microbial phytase at a level of 300 DPU was effective to spare about 20% of NPP in laying hen diets without any adverse effects on production performances and bone quality.

• Asian-Australasian Journal of Animal Sciences
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• 제21권10호
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• pp.1486-1494
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• 2008

The objective of this experiment was to compare the effects of green tea by-product and green tea probiotics on the growth performance, meat quality and immune response of finishing pigs. A total of 72 crossbred "Landrace$\times$Yorkshire" finishing pigs with an average of 76 kg body weight were assigned to 4 dietary treatments in a completely randomized design. Each treatment had 3 replications with 6 pigs per replication. The four dietary treatments were control, antibiotics (control diet with 0.003% chlortetracycline added), and diets containing 0.5% green tea by-product or 0.5% green tea probiotic supplementation. Weight gain was increased in 0.5% green tea probiotics treatment compared to others, but there was no significant difference (p>0.05). The incorporation of 0.5% green tea probiotics to diets reduced the feed conversion ratio in finishing pigs (p>0.05). The incorporation of 0.5% green tea by-product into the pig diet reduced the crude protein and fat contents of the meat (p>0.05). Pigs fed diets containing 0.5% green tea probiotic supplementation had lowered meat TBA values compared to those fed 0.5% green tea by-product (p<0.05). The proliferation of spleen cells stimulated with Con A (concanavalin: 0.1, 0.3, and $1.0{\mu}g/ml$) significantly increased with 0.5% green tea by-product treatment compared to antibiotic treatment (p<0.05), but was significantly decreased in 0.5% green tea probiotics treatment compared to the antibiotic treatment (p<0.05). When stimulated with $1.0{\mu}g/ml$ Con A, splenocyte production of IL-6 from pigs treated with 0.5% green tea by-product or green tea probiotics was significantly increased compared to the antibiotic treatment group (p<0.05). Splenocyte production of TNF-${\alpha}$ after treatment with $1.0{\mu}g/ml$ Con A was significantly higher following 0.5% green tea probiotics treatment (p<0.05), while TNF-${\alpha}$ production after $10.0{\mu}g/ml$ LPS (lipopolysaccharide) was significantly higher in the 0.5% antibiotic treatment group (p<0.05).

• Asian-Australasian Journal of Animal Sciences
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• 제21권9호
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• pp.1339-1347
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• 2008

The objective of this study was to determine the effects of green tea probiotics on growth performance, meat quality and immune response in finishing pigs, and to assess the possibility of substituting green tea probiotics for antibiotics in diets of finishing pigs. This green tea probiotics is made by mixing green tea powder and excipients (defatted rice bran and wheat bran) and fermenting the mixture with beneficial bacteria. A total of 90 crossbreed "Landrace$\times$Yorkshire" finishing pigs with an average body weight of $72.5{\pm}2.5kg$ were assigned to 5 dietary treatments in a completely randomized design. Each treatment had 3 replications with 6 pigs per replication. The five dietary treatments were control, antibiotic (0.003% chlortetracycline added) and 0.1, 0.5 and 1.0% of green tea probiotics. There were no significant differences in final body weight, daily weight gain, daily feed intake and feed conversion ratio in the green tea probiotics and antibiotic treatments (p>0.05). Crude protein content was significantly increased in the 0.1 and 1.0% green tea probiotics treatment groups (p<0.05) and there was no significant difference in crude fat content of the meat among the treatments. The TBA value of meat was significantly lowered with 0.5 and 1.0% green tea probiotics treatments compared to that of controls and statistically similar to the antibiotic treatment after 3 weeks of storage (p<0.05). The growth of spleen cells stimulated with Con A (0.1 and $1.0{\mu}g/ml$) was significantly increased with 1.0% green tea probiotics treatment compared to that of the control treatment (p<0.05). The growth of spleen cells stimulated with LPS (1.0, 3.0 and $10{\mu}g/ml$) was significantly increased in the 0.5% green tea probiotics group compared to the antibiotic group (p<0.05). In Con A ($1.0{\mu}g/ml$) medium, IL-6 production of spleen cells was significantly increased with 1.0% green tea probiotics treatment compared to that of the control (p<0.05). In LPS ($10.0{\mu}g/ml$) medium, TNF-${\alpha}$ production of spleen cells increased significantly in all green tea probiotics treatment groups compared to that of the control (p<0.05). Finally it can be summarized that addition of green tea probiotic has a positive effect similar to antibiotic and 0.5% is the suitable dietary supplementation dose for finishing pig production.

• 유기물자원화
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• 제14권4호
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• pp.121-131
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• 2006

본 연구는 산란계에서 남은 음식물과 녹차 부산물이 산란계의 산란성적과 계란품질에 미치는 영향을 연구하였다. 공시동물은 50주령 Tetran Brown종 산란계 210수를 7처리 5반복 (반복당 6수)으로 임의 배치하였다. 시험설계는 대조구와 옥수수 곡류와 대두박을 대용한 건조된 남은음식물 10, 20, 30 및 40% 첨가구 (10% DLF, 20% DLF, 30% DLF 및 40% DLF)와 1.0%의 녹차 부산물 첨가구 (1.0% GTB)와 30% DLF와 1.0% GTB를 첨가한 혼합 첨가구를 두어 사양실험을 8주 동안 실시하였다. 산란율은 대조구에 비하여 10, 20 및 40% DLF 처리구에서 유의적으로 증가하였다 (P<0.05). 난중은 대조구에 비하여 10% DLF 처리구에서 유의적으로 감소하였다 (P<0.05). 사료 섭취량은 대조구보다 20% DLF 처리구와 30% DLF와 1.0% GTB의 혼합 첨가구에서 높게 나타났다 (P<0.05). 사료 요구율은 대조구에 비하여 10% DLF 처리구와 1.0% GTB 처리구에서 유의적으로 감소하였다 (P<0.05). 난황 콜레스테롤은 DLF 처리구와 대조구 사이에서 변화가 없었다 (P>0.05). 난황의 리놀렌산은 DLF 처리구와 1.0% GTB 첨가한 처리구에서 유의적으로 증가하였다.

• Molecules and Cells
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• 제27권3호
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• pp.329-336
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• 2009

To evaluate the involvement of translation initiation factors eIF4E and eIFiso4E in Chilli veinal mottle virus (ChiVMV) infection in pepper, we conducted a genetic analysis using a segregating population derived from a cross between Capsicum annuum 'Dempsey' containing an elF4E mutation ($pvr1^2$) and C. annuum 'Perennial' containing an elFiso4E mutation (pvr6). C. annuum 'Dempsey' was susceptible and C. annuum 'Perennial' was resistant to ChiVMV. All $F_1$ plants showed resistance, and $F_2$ individuals segregated in a resistant-susceptible ratio of 166:21, indicating that many resistance loci were involved. Seventy-five $F_2$ and 329 $F_3$ plants of 17 families were genotyped with $pvr1^2$ and pvr6 allele-specific markers, and the genotype data were compared with observed resistance to viral infection. All plants containing homozygous genotypes of both $pvr1^2$ and pvr6 were resistant to ChiVMV, demonstrating that simultaneous mutations in elF4E and eIFiso4E confer resistance to ChiVMV in pepper. Genotype analysis of $F_2$ plants revealed that all plants containing homozygous genotypes of both $pvr1^2$ and pvr6 showed resistance to ChiVMV. In protein-protein interaction experiments, ChiVMV viral genome-linked protein (VPg) interacted with both eIF4E and eIFiso4E. Silencing of elF4E and eIFiso4E in the VIGS experiment showed reduction in ChiVMV accumulation. These results demonstrated that ChiVMV can use both eIF4E and eIFiso4E for replication, making simultaneous mutations in eIF4E and eIFiso4E necessary to prevent ChiVMV infection in pepper.

• Journal of Korean Medical Science
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• 제33권47호
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• pp.303.1-303.10
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• 2018

Background: Cell division cycle 6 (CDC6) is an essential regulator of DNA replication and plays important roles in the activation and maintenance of the checkpoint mechanisms in the cell cycle. CDC6 has been associated with oncogenic activities in human cancers; however, the clinical significance of CDC6 in prostate cancer (PCa) remains unclear. Therefore, we investigated whether the CDC6 mRNA expression level is a diagnostic and prognostic marker in PCa. Methods: The study subjects included 121 PCa patients and 66 age-matched benign prostatic hyperplasia (BPH) patients. CDC6 expression was evaluated using real-time polymerase chain reaction and immunohistochemical (IH) staining, and then compared according to the clinicopathological characteristics of PCa. Results: CDC6 mRNA expression was significantly higher in PCa tissues than in BPH control tissues (P = 0.005). In addition, CDC6 expression was significantly higher in patients with elevated prostate-specific antigen (PSA) levels (> 20 ng/mL), a high Gleason score, and advanced stage than in those with low PSA levels, a low Gleason score, and earlier stage, respectively. Multivariate logistic regression analysis showed that high expression of CDC6 was significantly associated with advanced stage (${\geq}T3b$) (odds ratio [OR], 3.005; confidence interval [CI], 1.212-7.450; P = 0.018) and metastasis (OR, 4.192; CI, 1.079-16.286; P = 0.038). Intense IH staining for CDC6 was significantly associated with a high Gleason score and advanced tumor stage including lymph node metastasis stage (linear-by-linear association, P = 0.044 and P = 0.003, respectively). Conclusion: CDC6 expression is associated with aggressive clinicopathological characteristics in PCa. CDC6 may be a potential diagnostic and prognostic marker in PCa patients.

• 한국산학기술학회논문지
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• 제22권5호
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• pp.509-516
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• 2021

본 연구는 Cysteamine hydrochloride (CSH)의 사료 내 첨가 급여가 육용계의 생산성 및 소화율에 미치는 영향을 조사하기 위해 수행되었다. 1 일령의 Ross broiler 수컷 180 수를 공시하여 3 개의 실험그룹에 각각 3 반복으로 반복 당 20 수씩 배치하였다. 실험설계는 Control (Basal diets), CSH-1 (Basal diets+250 mg CSH/kg feed), CSH-2 (Basal diets+500 mg CSH/kg feed)으로 디자인하였다. 육성 초기 동안의 일당 사료섭취량, 체중, 일당 증체량, 사료요구율, 육성률은 모든 실험그룹들 사이에서 차이가 나타나지 않았다. 육성 후기와 전체 실험기간 동안의 결과를 종합적으로 평가한 경우, CSH-1과 CSH-2 그룹은 Control 그룹과 비교하여 사료요구율이 유의하게 개선되는 것으로 나타났다(p<0.05). 한편, CSH의 사료 내 첨가는 육용계에 있어서 도체율과 정육률(가슴근육, 다리근육)에는 영향을 미치지 않는 것으로 나타났다. 유기물의 소화율은 CSH-2 그룹이 대조구에 비하여 높은 경향으로 나타났으나, 통계적인 유의차는 인정되지 않았다(p<0.1). 본 연구의 결과는 사료 내 250-500 mg/kg의 CSH 첨가 급여는 육용계의 사료요구율 및 유기물소화율을 개선시킬 수 있음을 시사한다.

• 한국초지조사료학회지
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• 제42권4호
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• pp.264-269
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• 2022

본 연구에서는 미생물 첨가에 따라 거세한우 비육우 분의 이화학적 특성, 미생물 성상, 가스발생량 및 퇴비 부숙도에 미치는 영향을 규명하고자 수행하였다. 이상의 결과를 종합하면, 4주 후, 미생물 첨가구에서 수분, 유기물, 총질소 함량 및 pH가 낮았으며, 나머지 이화학적 특성에서는 차이가 나타나지 않았다. 유산균과 효모균 수는 증가하였으며, 대장균 수는 감소하였다. 12주 후, 미생물 첨가구에서 유산균과 고초균 수는 높았으나, 수분, 효모 및 대장균 수는 낮게 낮았다. 하지만 암모니아, 황화수소 발생량과 퇴비 부숙도는 미생물 첨가에 의한 효과가 나타나지 않았다. 따라서, 거세한우 분에 미생물을 첨가하면 유익균은 증가하고, 병원성 미생물은 감소하여, 비육우의 생산성은 증진될 것으로 사료되지만, 가스 발생량 및 퇴비 부숙도에 대한 추가적인 연구는 지속적으로 수행되어야 할 것으로 사료된다.

• 한국가금학회지
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• 제38권4호
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• pp.331-338
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• 2011

본 시험은 산란형 토종닭 실용계의 산란능력을 보고자 실시하였다. 공시계는 국립축산과학원에서 생산된 종란을 인수하여 4가지 교배 조합으로 생산된 교잡종 360수로서, 20주령부터 64주령까지 사양 시험을 실시하면서 산란 능력을 조사하였다. 교배 조합은 A) C계통${\times}$Y계통${\times}$C계통, B) C계통${\times}$L계통${\times}$C계통, C) C계통${\times}$G계통${\times}$C계통, D) C계통${\times}$W계통${\times}$C계통으로 4교배 조합, 교배 조합당 6반복, 반복당 15수씩 완전 임의배치하였다. 시산 일령과 시산 난중은 교배 조합간 유의차는 없었다. 시산시의 체중은 D 교배종이 1,319 g으로 다른 교배종들에 비해 낮게 나타났다(P<0.05). 주령별 체중은 B 교배종이 20, 30 및 40주령에서 가장 높았고, D 교배종이 가장 낮게 나타났다(P<0.05). 평균 사료 섭취량은 B교배종이 주로 높게 나타났으며, 20~24주령, 32~44주령 및 52~60주령에서 높았다(P<0.05). 44주령 이후로는 D 교배종의평균사료섭취량이가장낮게나타났다(P<0.05). 24주령 이후부터60주령까지의평균난중은B 교배종이높았다(P<0.05). 산란율은 C 교배종이 시험기간 동안 다른 계통에 비해 높았다(P<0.05). 64주령까지의 산란수는 A, B, C, D 교배종이 각각 241.4, 235.6, 232.3 및 227.0개로서 차이를 나타내었으나(P<0.05), 28주령 이후부터는 교배 조합간 차이를 보이지 않았다. 20~24주령의 사료 요구율은 A와 C 교배종이 낮았다(P<0.05). 산란 말기인 60~64주령에서는 C 교배종의 사료 요구율이 가장 낮았다. 본 시험의 결과는 유정란 생산용 및 관상용 산란형 토종닭 생산을 위한 기초 자료를 제공함으로써 토종 실용계의 보급에 도움이 되리라 사료된다.