• Journal of Life Science
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• v.24 no.3
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• pp.234-241
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• 2014

To identify and isolate anticancer active compounds from Solanum nigrum, S. nigrum was extracted with MeOH and then fractionated with various organic solvents ($CH_2Cl_2$, EtOAc, n-BuOH, and $H_2O$). The cytotoxic effects of the MeOH extracts from S. nigrum and its organic solvent-soluble fractions were also tested in HT29 cells. All the MeOH extracts of S. nigrum and its organic-solvent extracts induced cytotoxicity in the HT29 cells. Among the extracts, $H_2O$ was the most effective. The $H_2O$ extract was purified further by repeated silica gel, Sephadex LH-20, Diaion HP- 20, and RP-18 column chromatography. An active anticancer compound, Des-N-26-methylene-dihydrotomatidine, was isolated with a molecular weight of 416 and a molecular formula of $C_{28}H_{48}O_2$. Analysis of the cytotoxic effects of Des-N-26-methylene-dihydrotomatidine on the HT29 cells compared to those of tomatine and tomatidine are similar in its structure, is higher than tomatidine above the 40 ${\mu}g/ml$ concentration, but lower than tomatine. This is the first study to describe the anticancer activity of Des-N-26-methylene-dihydrotomatidin, isolated from S. nigrum. Des-N-26- methylene-dihydrotomatidine seems to have potential as a natural bioactive compound.

• Journal of muscle and joint health
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• v.7 no.2
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• pp.281-293
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• 2000

This study was conducted to identify the relationships among self-care agency, family support, qualify of life in patients with rheumatoid arthritis. The subjects were 120 rheumatoid arthritis patients who attended RA O.P.D. at a university hospital, located in Daegu city, from 10th of May in 1998 to 30th of July in 1998. Three structure questionnaires were administered for explore three main research variables; 소향숙's self-care agency scale, 강현숙's family support scale, and 김종임's quality of life scale of RA patien. Data analysis was conducted with SPSS program including percentage, mean, standard deviation, mean score, Pearson Correlation Coefficient, multiple regression, and Cronbach's Alpha. Results are summarized as follows; 1. The mean of RA patient's self-care agency was 136.62, the mean of family support 37.38, and the mean of qualify of life 134.41. 2. Subject's self-care agency was positively correlated with family support(r=.2446) and with quality of life(r=.4341). Subject's family support was positively correlated with quality of life(r=.2630). 3. Stepwise multiple regression was used to determine the predictors of subject's quality of life. Significant predictors for subject's quality of life were self-care agency(t=4.873 p=0.0000), family support(t=4.480 p=0.0000) and the severity of arthralgia(t=-3.838, p=0.0002). The number of joints involved and the periods of illness did not show significant contribution to subject's quality of life. Self-care agency, family support, and family support explained 40. 39% of the variance in RA patient's quality of life. Given this results, it is suggested that a repeated study to measure RA patient's self-care practice in their own life may be needed to develope and validate an optimum level of nursing intervention for RA patient with which family support will be encouraged and patient's self-care agency will be facilitated.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.2
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• pp.155-161
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• 2002

Tetraselmis suecica (T. suecica) of Prasinophyta was selected because the growth rate is comparatively higher and the culturing is also easy. In order to investigate antioxidative activity, the soluble elements of T. suecica were fractionated using water and organic solvents such as methanol, hexane, chloroform, ethylacetate and butanol. The chloroform fraction of T. suecica showed strong antioxidative activity. The potential antioxidative activity was detected in hexane: ethylacetate (1:5) once used the fractions by different mixtures of organic solvents. This fraction was further purified by preparative thin layer chromatography (PTLC) and repeated reverse-phase HPLC. On the basis of chemical and spectroscopic evidences obtained by UV, FT-IR, FAB-MS and NMR, the compound purified from T. suecica was identified as pheophorbide-a. The antioxidative activity of the compound was comparable to that of $\alpha$-tocopherol and could be act as an antioxidant in foods.

• Korean Journal of Plant Taxonomy
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• v.41 no.4
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• pp.299-314
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• 2011

The choice of molecular markers is the first step when selecting experimental plans in the field of population genetics. The popular molecular markers in population genetic studies are mainly allozyme, RAPD, RFLP, AFLP, microsatellite, SNP and ISSR. Among these, microsatellites are frequently found in nuclear, chloroplast and mitochondrial genome, showing a high level of polymorphism and nuclear microsatellites are codominant. Thus, it is a favorable molecular marker for population structure analyses and genetic diversity studies. Microsatellites are composed of tandem repeated 1~6 base pair nucleotide motifs and can be easily amplified by PCR reactions using locus specific primers. Because microsatellites have low cross-species transferability, however, they are only applicable between phylogenetically close species. In wild plants, the lack of genomic information and the high development cost of the microsatellite obstruct the wider use of microsatellites in plant population genetics research. In this review, we introduce the basis for microsatellite markers, the development process, and analytical methods as well as evolutionary models and their applications. In addition, possible genotyping errors which lead to erroneous conclusions are discussed.

• Physical Therapy Korea
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• v.23 no.3
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• pp.11-20
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• 2016

Background: A forward head posture (FHP) is one of the most common types of poor head posture in patients with neck disorder. A prolonged FHP might increase pressure on the posterior cranio-cervical structure and exhibit reduced performance on a cranio-cervical flexion test (CCFT). CCFT is included to activate deep cervical flexor muscles and inhibit excessive activation of superficial cervical flexor muscles. Therefore, the selective activation of deep cervical flexors is needed for effective exercise for FHP. Objects: The purpose of this study was to compare muscle thickness between longus colli (Lco) and sternocleidomastoid (SCM) using ultrasonography in subjects with FHP depending on head support. Methods: This was a cross-sectional, case-control research design study. The ultrasonographic images of Lco and SCM were taken in 17 subjects with FHP during the 5 phases of the CCFT with and without a head support. Towel was used for supporting head to make the neutral head position in supine. Changes in muscle thickness during the test were calculated to infer muscle activation. Data were analyzed using repeated measures of two-way analysis of variance with the significance level of .05. Results: When subjects performed the CCFT with head support, there was a significant difference in muscle thickness of Lco and SCM (p<.05). According to a post hoc paired t-test, change of thickness of Lco was greater at all phases, and change of thickness of SCM muscle was less at phase 4 and 5 in condition with head support (p<.01) compared to condition without head support (p<.01). Conclusion: The result of this study suggest that applying head support for neutral head position during CCFT could be a useful method for activating Lco muscle without excessive activation of SCM muscle.

• Journal of the Korea Concrete Institute
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• v.24 no.3
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• pp.259-266
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• 2012

A general earthquake resistant design philosophy of ductile frame buildings allows beams to form plastic hinges adjacent to beam-column connections. In order to carry out this design philosophy, the ultimate bond or shear strength of the beam should be greater than the flexural yielding force and should not degrade before reaching its required ductility. The behavior of RC members dominated by bond or shear action reveals a dramatic reduction of energy dissipation in the hysteretic response due to the severe pinching effects. In this study, a method was proposed to predict the deformability of reinforced concrete members with short-span-to-depth-ratios, which would result in bond failure after flexural yielding. Repeated or cyclic loading produces a progressive deterioration of bond that may lead to failure at lower cyclic bond stress levels. Accumulation of bond damage is caused by the propagation of micro-cracks and progressive crushing of concrete in front of the lugs. The proposed method takes into account bond deterioration due to the degradation of concrete in the post yield range. In order to verify bond deformability of the proposed method, the predicted results were compared with the experimental results of RC members reported in the technical literature. Comparisons between the observed and calculated bond deformability of the tested RC members showed reasonably good agreement.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.33 no.2
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• pp.81-93
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• 2020

The fatigue characteristics of glass fiber reinforced plastic (GFRP) composites were studied under repeated loads using the finite element method (FEM). To realize the material characteristics of GFRP composites, Digimat, a mean-field homogenization tool, was employed. Additionally, the micro-structures and material models of GFRP composites were defined with it to predict the fatigue behavior of composites more realistically. Specifically, the fatigue characteristics of polybutylene terephthalate with short fiber fractions of 30wt% were investigated with respect to fiber orientation, stress ratio, and thickness. The injection analysis was conducted using Moldflow software to obtain the information on fiber orientations. It was mapped over FEM concerned with fatigue specimens. LS-DYNA, a typical finite element commercial software, was used in the coupled analysis of Digimat to calculate the stress amplitude of composites. FEMFAT software consisting of various numerical material models was used to predict the fatigue life. The results of coupled analysis of linear and nonlinear material models of Digimat were analyzed to identify the fatigue characteristics of GFRP composites using FEMFAT. Neuber's rule was applied to the linear material model to analyze the fatigue behavior in LCF regimen. Additionally, to evaluate the morphological and mechanical structure of GFRP composites, the coupled and fatigue analysis were conducted in terms of thickness.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.5
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• pp.503-509
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• 2016

To identify causes for drastic decrease in yield of tomato with repeated culturing number of the bag culture substrate of greenhouse tomatoes we investigated the physical and chemical properties of a coir used as the bag culture substrate to grow tomato at the Booyeo tomato experimental institute located in Booyeo, Chungnam Province for three years from 2012 to 2014. The results showed that total porosity ranged from 65.4 to 73.1% for the bulk densities of coir ranging from 0.12 to $0.14g\;cm^{-3}$. The volumetric water contents measured at 0.01 bar as air entry point were 25% (before), 33% ($1^{st}yr$), 45% ($2^{nd}yr$), and 37% ($3^{rd}yr$). Organic matter contents ranged from 82.0 to 96.2% (highest in $1^{st}yr$). pH and EC ranged from 4.47 to 6.47 (highest in $2^{nd}yr$), and from 22.2 to $53.5dS\;m^{-1}$ (highest in $1^{st}yr$) and cation exchange capacity ranged from 71.0 to $191.7cmol\;kg^{-1}$ (highest in $3^{rd}yr$). The surface structure observed with electrical microscope showed that the number of large pores decreased with increasing cultivating time while the proportion of smaller pores increased, indicating that the coir was consistently decomposed. Therefore, we could conclude that these changes of all physical and chemical properties of the coir may influence the holding capacities of water and nutrients, resulting in deterioration of quality of culture substrate of greenhouse tomatoes.

• Journal of the Korean Society for Railway
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• v.19 no.2
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• pp.135-144
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• 2016

It is essential to lighten the weight of switch girders in order to reduce their costs of manufacturing and make it easier to use them in construction. Lightening the weight of switch is also important to the Maglev 3-way switches system, however, the design variables should be considered very carefully if lightening is to be applied to the system, because these variables are vitally related to the levitation stability. Because Urban Maglev trains have a structure in which train bogie wraps around the guiderail, the adjustment of a girder's height is a possible way to reduce the weight. The safety of the application of this concept is ensured by repeated experiments in a test bed, however, due to a lack of space and budget limits, the design parametric study for the system model can substitute for actual application. The purpose of this paper is to study the design parameters that are concerned with levitation stability while a Maglev train is running on the Maglev 3-way system depending on the weight of the switch girders. In this study, switch girder weight is reduced by adjustment of girder height and girders are and modeled as a flexible body. The effect of the adjustment of girder height on the levitation stability can be analyzed by comparing the velocity of the train when it passes the switch girders, with the lateral gap, and the levitation gap which are obtained from the co-simulation of the Maglev train's dynamics model and flexible switching system. The results of this research will be used to design a Maglev switch.

• Journal of the Korean Electrochemical Society
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• v.14 no.1
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• pp.16-21
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• 2011

$Li_4Sn_xTi_{5-x}O_{12}$ was manufactured by high energy ball milling (HEBM) and used as an anode material for lithium ion battery. Various amount of $SnO_2$was added to $Li_4Ti_5O_{12}$ and heated at different temperatures. The purpose of this research was to see the effect of $SnO_2$ addition into $Li_4Ti_5O_{12}$. Manufactured samples were analyzed by TGA, XRD, SEM, PSA. Battery cycler was used to test the charge/discharge properties of active materials. Heat treatment temperature of $800^{\circ}C$ was needed to make a stable structure of $Li_4Sn_xTi_{5-x}O_{12}$ and the particle size distribution was $0.2{\sim}0.6\;{\mu}m$. Charge/discharge process was repeated for 50 cycles at room temperature. The initial capacity was 168mAh/g and the voltage plateau was observed at 1.55V(Li/$Li^+$).