• Title/Summary/Keyword: Repeat System

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Development of Reusable Split URA3-Marked Knockout Vectors for Saccharomyces cerevisiae

  • Lee Tae-Hee;Kim Myoung-Dong;Seo Jin-Ho
    • Journal of Microbiology and Biotechnology
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    • v.16 no.6
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    • pp.979-982
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    • 2006
  • Two knockout vectors, in which the truncated Kluyveromyces lactis URAS gene is flanked by a direct repeat, were developed for Saccharomyces cerevisiae. Each vector was designed to harbor 5'- and 3'-end homology regions for integration. Two knockout fragments were devised to integrate into the correct locus in a complementary manner to disrupt a gene of interest and. concomitantly to make functional Kl URA3 for transfomant selection. The use of dual complementary knockout cassettes was expected to dramatically reduce integration into unwanted loci in the genome. The knockout system developed in this study was successfully used for disruption of the GAL1 gene in S. cerevisiae.

A Study on the Optimum Design using Finite Element Analysis and Automatic Design of Lachet Wheel (라체트 휠의 자동설계와 유한요소해석을 이용한 최적화에 관한 연구)

  • 박진형;이승수;김민주;김태호;전언찬
    • Proceedings of the Korean Society of Machine Tool Engineers Conference
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    • 2001.04a
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    • pp.3-8
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    • 2001
  • This study is an investigation for the ADS optimum design by using FEA. We write out program which express ADS perfectly and reduce the required time for correcting of model to the minimum in solution and manufacture result. We complete algorithm which can plan optimum forming of model by feedback error information in CAE. Then we correct model by feedback date obtaining in solution process, repeat course following stress solution again and do modeling rachet wheel for optimum forming. That is our aim. In rachet wheel, greatest equivalence stress originates in key groove corner and KS standard is proved the design for security.

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A Study on the Selection of Optimum Auto-design Data using FEA (유한요소해석을 이용한 최적자동설계 데이터 선정에 관한 연구)

  • 박진형;이승수;김민주;김순경;전언찬
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2001.04a
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    • pp.406-409
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    • 2001
  • This study is an investigation for the ADS optimum design by using FEA. We write out program which express ADS perfectly and reduce the required time for correcting of model to the minimum in solution and manufacture result. We complete algorithm which can plan optimum forming of model by feedback error information in CAE. Then we correct model by feedback date obtaining in solution process, repeat course following stress solution again and do modeling rachet wheel for optimum forming. That is our aim. In rachet wheel, greatest equivalence stress originates in key groove corner and KS standard is proved the design for security.

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Design and Implementation of Multimedia Teaching Aids for the Effective English Learning (효과적인 영어학습을 위한 멀티미디어 학습 도구의 설계 및 구현)

  • Kim, Jee-Won;Lee, Jung-Sun;Ahn, Sung-Eun;Choi, Hwang-Kyu
    • Journal of Industrial Technology
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    • v.21 no.A
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    • pp.135-139
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    • 2001
  • There has been a study about the effective multimedia education using a computer following the appearance of a virtual space. Also, there has been an effort to connect the information & communication technology with education. The popular on-line lecture systems are mostly on English lecture sites. However, they just offer the VOD(Video On Demand) services ignoring students' convenience. To improve these week points, we design and implement the multimedia leaching system focusing on an efficient repeat-effect in order that students can control the Media Player by clicking a sentence on a web page. This paper presents the Editor and Player considering students' interest and the effective learning fruits. So users can easily make multimedia materials and use them to improve their English listening skill.

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An Algorithm for Iterative Detection and Decoding MIMO-OFDM HARQ with Antenna Scheduling

  • Kim, Kyoo-Hyun;Kang, Seung-Won;Mohaisen, Manar;Chang, Kyung-Hi
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.2 no.4
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    • pp.194-208
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    • 2008
  • In this paper, a multiple-input-multiple-output (MIMO) hybrid-automatic repeat request (HARQ) algorithm with antenna scheduling is proposed. It retransmits the packet using scheduled transmit antennas according to the state of the communication link, instead of retransmitting the packet via the same antennas. As a result, a combination of conventional HARQ systems, viz. chase combining (CC) and incremental redundancy (IR) are used to achieve better performance and lower redundancy. The proposed MIMO-OFDM HARQ system with antenna scheduling is shown to be superior to conventional MIMO HARQ systems, due to its spatial diversity gain.

Fingerprint Classification and Identification Using Wavelet Transform and Correlation (웨이블릿변환과 상관관계를 이용한 지문의 분류 및 인식)

  • 이석원;남부희
    • Journal of Institute of Control, Robotics and Systems
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    • v.6 no.5
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    • pp.390-395
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    • 2000
  • We present a fingerprint identification algorithm using the wavelet transform and correlation. The wavelet transform is used because of its simple operation to extract fingerprint minutiaes features for fingerprint classification. We perform the rowwise 1-D wavelet transform for a $256\times256$ fingerprint image to get a $1\times256$ column vector using the Haar wavelet and repeat 1-D wavelet transform for a 1$\times$256 column vector to get a $1\times4$ feature vector. Using PNN(Probabilistic Neural Network), we select the possible candidates from the stored feature vectors for fingerprint images. For those candidates, we compute the correlation between the input binary image and the target binary image to find the most similar fingerprint image. The proposed algorithm may be the key to a low cost fingerprint identification system that can be operated on a small computer because it does not need a large memory size and much computation.

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New Approach to the Analysis of Palindromic Structure in Genome Sequences

  • Kim, Seok-Won;Lee, Yong-Seok;Choi, Sang-Haeng;Chae, Sung-Hwa;Kim, Dae-Won;Park, Hong-Seog
    • Genomics & Informatics
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    • v.4 no.4
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    • pp.167-169
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    • 2006
  • PABAP (Palindrome Analysis by BLAST Program) is an analysis system that identifies palindromic sequences from a large genome sequence up to several megabases long. It uses NCBI BLAST as a searching engine, and data processing such as alignment filtration and detection of inverted repeats which satisfy user-defined parameters is performed by manipulating data after populating into a MySQL database. PABAP outperforms publicly available palindrome search program in that it can detect large palindrome with internal spacer at a faster speed from bacterial genomes. It is a standalone application and is freely available for noncommercial users.

A Study on the Verification Using Finite Element Analysis and Automatic Design of Ratchet Wheel (래칫 휠의 자동설계와 유한요소해석을 이용한 검증에 관한 연구)

  • 김민주;이승수;전언찬
    • Transactions of the Korean Society of Machine Tool Engineers
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    • v.11 no.3
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    • pp.45-50
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    • 2002
  • This study is an investigation far the Am optimum design using FEA. We write out program which express ADS perfectly and reduce the required time far correcting of model to the minion in solution md manufacture result. We complete algorithm which can plan optimum forming of model by feedback error information in CAE. Then we contract model by feedbback date obtaining in solution process, repeat course following stress solution again iud do modeling rachet wheel for optimum forming. That is our aim. In cachet wheel, greatest equivalence strss originates in key groove comer and KS standard is proved the design far security.

Surface Damage Accumulation in Alumina under the Repeated Normal-Tangential Contact Forces

  • Lee, Kwon-Yong;Choi, Sung-Jong;Youn, Ja-Woong
    • KSTLE International Journal
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    • v.1 no.1
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    • pp.48-51
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    • 2000
  • Surface damage accumulation of alumina ceramics under the cyclic stress state was analyzed. The alternating stress state in repeat pass sliding contact was simulated by a synchronized biaxial (normal and tangential) repeated indentation technique. Wear debris formation mechanism through damage accumulation and fatigue grain failure in both alumina ceramic balls and flat disks was confirmed, and the contact induced surface degradation due to fatigue cracking accumulation was quantified by measuring vertical contact displacement. Variation of structural compliance (slope of load-displacement curve) of two contacting bodies was expressed as a variation of the apparent elastic property, called pseudo-elastic constant, of the contact system.

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Genetic Polymorphisms of t-PA and PAI-1 Genes in the Korean Population

  • Kang, Byung-Yong;Lee, Kang-Oh
    • Animal cells and systems
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    • v.7 no.3
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    • pp.249-253
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    • 2003
  • Abnormalities in fibrinolysis system is associated with risk of hypertension. In this report, the Alu repeat insertion/deletion (I/D) polymorphism of tissue plasminogen activator (t-PA) and the Hind III RFLP of plasminogen activator inhibitor-1 (PAI-1) genes were investigated in 115 normotensives and 83 patients with hypertension, and their association with anthropometrical data and plasma biochemical parameters were analyzed. There were no significant differences in the gene frequencies of the two candidate genes between normotensives and hypertensives, respectively. Our results indicate lack of associations between the two polymorph isms in t-PA and PAI-1 genes and risk of hypertension in the population under study. However, the Hind III RFLP of PAI-1 gene was significantly associated with plasma glucose level, suggesting its role in glucose metabolism. It needs to be tested whether this RFLP of PAI-1 gene is associated with insulin resistance syndrome or non-insulin dependent diabetes mellitus (NIDDM) in the Korean population.