• Animal Bioscience
• /
• v.36 no.2_spc
• /
• pp.333-338
• /
• 2023

Genetic modification enables modification of target genes or genome structure in livestock and experimental animals. These technologies have not only advanced bioscience but also improved agricultural productivity. To introduce a foreign transgene, the piggyBac transposon element/transposase system could be used for production of transgenic animals and specific target protein-expressing animal cells. In addition, the clustered regularly interspaced short palindromic repeat-CRISPR associated protein 9 (CRISPR-Cas9) system have been utilized to generate chickens with knockout of G0/G1 switch gene 2 (G0S2) and myostatin, which are related to lipid deposition and muscle growth, respectively. These experimental chickens could be the invaluable genetic resources to investigate the regulatory pathways and mechanisms of improvement of economic traits such as fat quantity and growth. The gene-edited animals could also be applicable to the livestock industry.

• Journal of Biosystems Engineering
• /
• v.29 no.2
• /
• pp.159-166
• /
• 2004

In this study, we have developed a web-based management system for greenhouse teleoperation. The remote control system consisted of a database, a web-server, a controller in greenhouse, and clients. The database in the server stored user's information and greenhouse conditions was used to manage user's login and conditioning data. The management system developed by using Java applet, which was a client program for effective and easy management of greenhouse, monitored the greenhouse in real time. Master and driver boards were installed in the greenhouse control unit. Database on flowering to collect and analyze data exchanged data with the server. The master board could be managed effectively by timer routine, repeat control within setting time, and algorithm of setting points. Also, the greenhouse conditions could be controlled by manual or remote controller(PC) through a web browser in internet. Furthermore, all of the control devices of the greenhouse were managed by remote control of using PC and checked via camera installed in greenhouse. Finally, we showed the experimental results of the system which was installed in Pusan Horticultural Experiment Station.

• Journal of Radiation Protection and Research
• /
• v.22 no.3
• /
• pp.161-170
• /
• 1997

Theoretical and experimental determination of the lower limits of detection (LLD) of C-300-A $CaSO_4$:Dy TL dosimeters which are currently used for the personnel monitoring in Korea Atomic Energy Research Institute(KAERI) are described with a critical level which is defined as the signal level above which a result has a probability of being due to a fluctuation of the background. The personnel monitoring processors can derived easily the LLD of their system using this method with the background readings of their service interval and the irradiation readings of the known doses. Experimental studies were also conducted for the fading rates of the dosimeters with the temperatures and humidities for 3 months. Finally sensitivity changes in repeat uses were measured for 40 times consecutive uses of the dosimeters. The applications of the experimental results of fading rates and sensitivity changes in real personnel monitoring services are discussed briefly.

• Investigative and Clinical Urology
• /
• v.59 no.6
• /
• pp.363-370
• /
• 2018

Purpose: The aim of this study is to describe the technique and to report early results of transperineal magnetic resonance imaging and ultrasonography (MRI-US) fusion biopsy. Materials and Methods: A total of 75 patients underwent MRI-US fusion transperineal biopsy. Targeted biopsy via MRI-US fusion imaging was carried out for cancer-suspicious lesions with additional systematic biopsy. Detection rates for overall and clinically significant prostate cancer (csPCa) were evaluated and compared between systematic and targeted biopsy. In addition, further investigation into the detection rate according to prostate imaging reporting and data system (PI-RADS) score was done. Results of repeat biopsies were also evaluated. Results: Overall cancer detection rate was 61.3% (46 patients) and the detection rate for csPCa was 42.7% (32 patients). Overall detection rates for systematic and targeted biopsy were 41.3% and 57.3% (p<0.05), respectively. Detection rates for csPCa were 26.7% and 41.3%, respectively (p<0.05). The cancer detection rates via MRI fusion target biopsy were 30.8% in PI-RADS 3, 62.1% in PI-RADS 4 and 89.4% in PI-RADS 5. Rates of csPCa missed by targeted biopsy and systematic biopsy were 0.0% and 25.0%, respectively. The cancer detection rate in repeat biopsies was 61.1% (11 among 18 patients) in which 55.5% of cancer suspected lesions were located in the anterior portion. Conclusions: Transperineal MRI-US fusion biopsy is useful for improving overall cancer detection rate and especially detection of csPCa. Transperineal MRI-US targeted biopsy show potential benefits to improve cancer detection rate in patients with high PIRADS score, tumor located at the anterior portion and in repeat biopsies.

• Journal of Korean Society for Quality Management
• /
• v.46 no.3
• /
• pp.411-424
• /
• 2018

Purpose: The purpose of this study is to improve submarine TACM launcher system safety device performance. Methods: In this study, EPLD(Electrically Programmable Logic Device) control and time sharing method to the safety device actuator motor and discrete signal processor in launch control panel were used to resolve unusual performance of safety system. Results: The result of this study are as follows; First, sporadic stopping of safety device actuator motor due to insufficient In-Rush current was resolved. Second, repeat of safety device condition as lock & release due to chattering for motor activating was resolved. Third, simultaneous release function for safety device actuator was available. Conclusion: The unusual performance of function for submarine TACM launcher system was overcame by applying EPLD control and time sharing method. The suggestions were proved by performance test in the pressure chamber. The results of this study enhanced survivability of ${\bigcirc}{\bigcirc}{\bigcirc}$ class submarine from enemy torpedo.

• Atmosphere
• /
• v.30 no.2
• /
• pp.103-113
• /
• 2020

In this study, we analyzed and developed the monitoring system in order to confirm the effect of observations on forecast sensitivity on ensemble-based data assimilation. For this purpose, we developed the Ensemble Forecast Sensitivity to observation (EFSO) monitoring system based on Local Ensemble Transform Kalman Filter (LETKF) system coupled with Korean Integrated Model (KIM). We calculated 24 h error variance of each of observations and then classified as beneficial or detrimental effects. In details, the relative rankings were according to their magnitude and analyzed the forecast sensitivity by region for north, south hemisphere and tropics. We performed cycle experiment in order to confirm the EFSO result whether reliable or not. According to the evaluation of the EFSO monitoring, GPSRO was classified as detrimental observation during the specified period and reanalyzed by data-denial experiment. Data-denial experiment means that we detect detrimental observation using the EFSO and then repeat the analysis and forecast without using the detrimental observations. The accuracy of forecast in the denial of detrimental GPSRO observation is better than that in the default experiment using all of the GPSRO observation. It means that forecast skill score can be improved by not assimilating observation classified as detrimental one by the EFSO monitoring system.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.24 no.9B
• /
• pp.1616-1623
• /
• 1999

This paper describes error resilient coding scheme is added in WLL system and its application for robust low-bit rate still image transmission over power controlled W-CDA system Rayleigh fading channels. The baseline JPEG compressing methods are uses in image coding over wireless channel. The channel uses Reed-Solomon(RS) outer codes concatenated with convolutional inner codes, and truncated type I hybrid ARQ protocol based on the selective repeat strategy and the RS error detection capability. Simulation results are proved for the statistics of the frame-error bursts of the proposed system in comparison with conventional WLL system. it gains the 2 dB of the Eb/No in same BER.

• BMB Reports
• /
• v.40 no.5
• /
• pp.740-748
• /
• 2007

To gain insight into the structure and function of repressor proteins of bacteriophages of gram-positive bacteria, repressor of temperate Staphylococcus aureus phage ${\phi}11$ was undertaken as a model system here and purified as an N-terminal histidine-tagged variant (His-CI) by affinity chromatography. A ~19 kDa protein copurified with intact His-CI (~ 30 kDa) at low level was resulted most possibly due to partial cleavage at its Ala-Gly site. At ~10 nM and higher concentrations, His-CI forms significant amount of dimers in solution. There are two repressor binding sites in ${\phi}11$ cI-cro intergenic region and binding to two sites occurs possibly by a cooperative manner. Two sites dissected by HincII digestion were designated operators $O_L$ and $O_R$, respectively. Equilibrium binding studies indicate that His-CI binds to $O_R$ with a little more strongly than $O_L$ and binding species is probably dimeric in nature. Interestingly His-CI binding affinity reduces drastically at elevated temperatures ($32-42^{\circ}C$). Both $O_L$ and $O_R$ harbor a nearly identical inverted repeat and studies show that ${\phi}11$ repressor binds to each repeat efficiently. Additional analyses indicate that ${\phi}11$ repressor, like $\lambda$ repressor, harbors an N-terminal domain and a C-terminal domain which are separated by a hinge region. Secondary structure of ${\phi}11$ CI even nearly resembles to that of $\lambda$ phage repressor though they differ at sequence level. The putative N-terminal HTH (helix-turn-helix) motif of ${\phi}11$ repressor belongs to the HTH -XRE-family of proteins and shows significant identity to the HTH motifs of some proteins of evolutionary distant organisms but not to HTH motifs of most S. aureus phage repressors.

• Korean Journal of Medicinal Crop Science
• /
• v.27 no.6
• /
• pp.376-382
• /
• 2019

Background: Angelica gigas Nakai has been used as an herbal medicine in Eastern Asia for treating disorders in women for a long time. To date there are no studies on the genetic diversity of A. gigas. The present study aimed to study the genetic diversity of A. gigas variants using genome-wide simple sequence repeat (SSR) markers. Methods and Results: The genetic diversity of 199 variants of A. gigas cultivated in of different regions, was analyzed using 5 genome-wide SSR markers. The results revealed that the genetic variants were very diverse, and genetic analysis using the 5 SSR markers revealed high diversity among the variants. Conclusions: It is expected that the development of the true Angleical cultivar, by studying the system and group selection, can be achieved by genetic analysis using the developed markers, for generating a genetically fixed lineage and group selection.

• Journal of Microbiology and Biotechnology
• /
• v.24 no.9
• /
• pp.1189-1195
• /
• 2014

A strain-specific identification method is required to secure Chlorella strains with useful genetic traits, such as a fast growth rate or high lipid productivity, for application in biofuels, functional foods, and pharmaceuticals. Microsatellite markers based on simple sequence repeats can be a useful tool for this purpose. Therefore, this study developed five novel microsatellite markers (mChl-001, mChl-002, mChl-005, mChl-011, and mChl-012) using specific loci along the chloroplast genome of Chlorella vulgaris. The microsatellite markers were characterized based on their allelic diversities among nine strains of C. vulgaris with the same 18S rRNA sequence similarity. Each microsatellite marker exhibited 2~5 polymorphic allele types, and their combinations allowed discrimination between seven of the C. vulgaris strains. The two remaining strains were distinguished using one specific interspace region between the mChl-001 and mChl-005 loci, which was composed of about 27 single nucleotide polymorphisms, 13~15 specific sequence sites, and (T)n repeat sites. Thus, the polymorphic combination of the five microsatellite markers and one specific locus facilitated a clear distinction of C. vulgaris at the strain level, suggesting that the proposed microsatellite marker system can be useful for the accurate identification and classification of C. vulgaris.