• Journal of Life Science
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• v.9 no.4
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• pp.341-347
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• 1999

Insects use chitinolytic enzyme to digest chitin in the exoskelton during the molting process. We have isolated and sequenced a chitinase-encoding cDNA from the silkworm, Bombyx mandarina, compared its sequenced with genes encoding chitinolytic enzymes from other sources. The insert DNA in the clone is 2,675 nucleotides long with an open reading frame of 1,695 uncletides that encodes a protein of 565 amino acids with a molecuar weight of 63.4 kDa. The 3' -untranslated region of 889 nucleotides is AT-rich and contains two putative polyadenylation signals. The N-terminal sequence of the encoded protein contains numerous hydrophobic residues characteristic of a leader peptide. The amino acid alignment revealed that the endo-$\beta$-N-acetylglucosaminidase had 83% and 97% homology with M. sexta and B. mori, respectively. The deduced amino acid had two highly conserved region at the amino acid residues 97-111 and 139-148 that were related to the existing chitinase.

• Journal of KIISE:Computer Systems and Theory
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• v.29 no.3
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• pp.117-124
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• 2002

This paper proposes a fault-tolerant multicasting algorithm employing the region encoding scheme in multistage interconnection networks (MIN's) containing multiple faulty switching elements. After classifying all switching elements into two subsets with equal sizes in MIN, the proposed algorithm can tolerate the faulty pattern where every fault is contained in the same subset. In order to send a multicast message to its destinations detouring faults, the proposed algorithm uses the recursive scheme that recirculates it through MIN, We prove that this algorithm can route any multicast message in only two passes through the faulty MIN.

• Journal of Microbiology
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• v.37 no.4
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• pp.229-233
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• 1999

The mitochondrial plasmid pMLP1 from a white-rot fungus, Pleurotus ostreatus, is a double-stranded DNA containing 381 bp terminal inverted repeat (TIR) whose 5'-ends are covalently bound by terminal proteins. The plasmid contains two major open reading frames (ORFs), encoding putative DNA and RNA polymerases, and a minor ORF encoding a small, highly basic protein. To identify the DNA binding activity that recognizes the TIR region of pMLP1, gel retardation assays were performed with mitochondrial extracts. A specific protein binding to a region between 123 and 248 nt within TIR was observed. We examined whether the gene product of mORF1 bindes to this region specifically. E. coli cell extract which contains an overproduced mORF1 protein formed a complex specific to the region between 123 and 248 nt. Inclusion of mORF1 protein in the specific complex formed between P. ostreatus mitochondrial extract and TIR was confirmed by a supershift assay using polyclonal antibodies against the mORF1 protein. Our result suggest that the product of mORF1 may function as a terminal region recognition factor (TRF), recognizing an internal region in TIR.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.27 no.6A
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• pp.575-583
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• 2002

JBIG2 is an International Standard fur compression of Bi-level images and documents. JBIG2 supports three encoding modes for high compression according to region features of documents. One of which is generic region coding for bitmap coding. The basic bitmap coder is either MMR or arithmetic coding. Pattern matching coding method is used for text region, and halftone pattern coding is used for halftone region. In this paper, a document is segmented into line-art, halftone and text region for JBIG2 encoding and JBIG2 CODEC is implemented. For efficient region segmentation of documents, region segmentation method using wavelet coefficient is applied with existing boundary extraction technique. In case of facsimile test image(IEEE-167a), there is improvement in compression ratio of about 2% and enhancement of subjective quality. Also, we propose arbitrary shape halftone region coding, which improves subjective quality in talc neighboring text of halftone region.

• Journal of Korea Multimedia Society
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• v.13 no.2
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• pp.185-195
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• 2010

In this paper, we propose an efficient inter and intra prediction algorithms for fast encoding of H.264/AVC. First, inter prediction mode decision method decides early using temporal/spatial correlation information and pixel direction information. Second, intra prediction mode decision method selects block size judging smoothness degree with inner/outer pixel value variation and decides prediction mode using representative pixel and reference pixel. Lastly, adaptive motion search region restriction sets search region using mode information of neighboring block and predicted motion vector. The experimental results show that proposed method can achieve about 18~53% reduction compared with the existing JM 14.1 in the encoding time. In RD performance, the proposed method does not cause significant PSNR value losses while increasing bitrates slightly.

• Proceedings of the Korea Institute of Convergence Signal Processing
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• 2000.12a
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• pp.125-128
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• 2000

The conventional fractal decoding was required a vast amount computational complexity. Since every range blocks was implemented to IFS(iterated function system). In order to improve this, it has been suggested to that each range block was classified to iterated and non-iterated regions. If IFS region is contractive, then it can be performed a fast decoding. In this paper, a searched region of the domain in the encoding is limited to the range region that is similar with the domain block, and IFS region is a minimum. So, it can be performed a fast decoding by reducing the computational complexity for IFS in fractal image decoding.

• Horticulture, Environment, and Biotechnology : HEB
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• v.59 no.6
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• pp.857-864
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• 2018

Previous studies have not detected transcripts of the gene encoding anthocyanidin synthase (ANS) in white pomegranates (Punica granatum L.) and suggest that a large-sized insertion in the coding region of the ANS gene might be the causal mutation. To elucidate the identity of the putative insertion, 3887-bp 5' and 3392-bp 3' partial sequences of the insertion site were obtained by genome walking and a gene coding for an expansin-like protein was identified in these genome-walked sequences. An identical protein (GenBank accession OWM71963) isolated from pomegranate was identified from BLAST search. Based on information of OWM71963, a 5.8-Mb scaffold sequence with genes coding for the expansin-like protein and ANS were identified. The scaffold sequence assembled from a red pomegranate cultivar also contained all genome-walked sequences. Analysis of positions and orientations of these genes and genome-walked sequences revealed that the 27,786-bp region, including the 88-bp 5' partial sequences of the ANS gene, might be translocated into an approximately 22-kb upstream region in an inverted orientation. Borders of the translocated region were confirmed by PCR amplification and sequencing. Based on the translocation mutation, a simple PCR codominant marker was developed for efficient genotyping of the ANS gene. This molecular marker could serve as a useful tool for selecting desirable plants at young seedling stages in pomegranate breeding programs.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1986.12a
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• pp.512.1-512
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• 1986

The cdd gene of Bacillus subtilis, encoding the deoxycytidinecytidine deaminase of pyrimidine nucleotide biosynthesis has been cloned into the EcoRl site of pBR322. The recombinant plasmid, pSol, promoted the synthesis of 100-140 fold elevated levels of the enzyme. A comparison of the polypeptides encoded by cdd complementing and non-complementing plasmids in the mini cell showed the gene product to have a molecular mass of approximately 14 kDa. The nucleotide sequence of the gene and 460 base pairs upstream from the coding region was determined. An open-reading frame, encoding a protein with a calculated molecular mass of 14337 Da, was deduced to be the coding region for cdd. However, the enzyme has an apparent molecular mass of 54 kDa as determined by gel filteration, whereas sucrose density gradient centrifugation shows 58 kDa. It means that the enzyme could be forming a tetramer in a physiological state. About 28 amino acids of the N-tetramer in a physiological state. About 28 amino acids of the N-terminal presumably form a signal for membrane translocation and six cystein residues are contained in the structure. S1 nuclease mapping indicated that transcription of cdd is initiated 17 base pairs upstream from the translational start. The structural characterization of the odd gene was performed.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.17 no.3
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• pp.213-224
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• 1992

An encoding technique based on region splitting and vector quantization is proposed for the lower level Laplacian pyramid images. The lower level Laplacian pyramid images have lower variance than higher levels but a great influence on compression ration due to large spatial area. And so from data compression viewpoint, we subdivide them with variance thresholding into two regions such as one called : flat region” and the other “edge region”, and encode the flat region with its mean value and the edge region as vector quantization method. The edge region can be reproduced faithfully and significant improvement on compression ratio can be accomplished with a little degradation of PSNR in spite of the effect of large flat region since the codebook used is generated from the edge region only on from the entire image including the flat region. It can be verified by computer simulation results that proposed method is more efficient in compression ratio and processing time than the conventional encoding technique of vector quantization.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 1986.12a
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• pp.512.2-512
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• 1986

A mutant of bacillus subtilis with a defective cdd gene encoding deoxycytidine-cytidine deaminase(EC 3.5.4.5.) has been characterized genetically. The genetic lesion causing the altered deoxycytidine-cytidine deaminase, cdd, was mapped at 225 min on the linkage map of B.subtilis by AR9 transduction Transductional analysis of the cdd region established the gene order as trp-lys-dnaE-cdd-aroD. The cdd gene was linked 72% with the aroD and 20% with the lys.