Horticulture, Environment, and Biotechnology : HEB

Korean Society of Horticultural Science (한국원예학회)
권호 표지
DOI QR코드

DOI QR Code

Identification of chromosomal translocation causing inactivation of the gene encoding anthocyanidin synthase in white pomegranate (Punica granatum L.) and development of a molecular marker for genotypic selection of fruit colors

  • Jeong, Hyeon-ju (Jeollanamdo Agricultural Research and Extension Service) ;
  • Park, Moon-Young (Jeollanamdo Agricultural Research and Extension Service) ;
  • Kim, Sunggil (Department of Plant Biotechnology, Biotechnology Research Institute, Chonnam National University)
  • Received : 2018.02.06
  • Accepted : 2018.04.24
  • Published : 2018.12.31
⟨ Previous Next ⟩

Abstract

Previous studies have not detected transcripts of the gene encoding anthocyanidin synthase (ANS) in white pomegranates (Punica granatum L.) and suggest that a large-sized insertion in the coding region of the ANS gene might be the causal mutation. To elucidate the identity of the putative insertion, 3887-bp 5' and 3392-bp 3' partial sequences of the insertion site were obtained by genome walking and a gene coding for an expansin-like protein was identified in these genome-walked sequences. An identical protein (GenBank accession OWM71963) isolated from pomegranate was identified from BLAST search. Based on information of OWM71963, a 5.8-Mb scaffold sequence with genes coding for the expansin-like protein and ANS were identified. The scaffold sequence assembled from a red pomegranate cultivar also contained all genome-walked sequences. Analysis of positions and orientations of these genes and genome-walked sequences revealed that the 27,786-bp region, including the 88-bp 5' partial sequences of the ANS gene, might be translocated into an approximately 22-kb upstream region in an inverted orientation. Borders of the translocated region were confirmed by PCR amplification and sequencing. Based on the translocation mutation, a simple PCR codominant marker was developed for efficient genotyping of the ANS gene. This molecular marker could serve as a useful tool for selecting desirable plants at young seedling stages in pomegranate breeding programs.

Keywords

Acknowledgement

Grant : Center for Horticultural Seed Development, Plant Molecular Breeding Center

Supported by : Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry (IPET)

References

  1. Andersen JR, Lubberstedt T (2003) Functional markers in plants. Trends Plant Sci 11:554-560
  2. Ben-Simhon Z, Judeinstein S, Nadler-Hassar T, Trainin T, Bar-Ya'akov I, Borochov-Neori H, Holland D (2011) A pomegranate (Punica granatum L.) WD40-repeat gene is a functional homologue of Arabidopsis TTG1 and is involved in the regulation of anthocyanin biosynthesis during pomegranate fruit development. Planta 234:865-881 https://doi.org/10.1007/s00425-011-1438-4
  3. Ben-Simhon Z, Judeinstein S, Trainin T, Harel-Beja R, Bar-Ya'akov I, Borochov-Neori H, Holland D (2015) A "White" anthocyaninless pomegranate (Punica granatum L.) caused by an insertion in the coding region of the leucoanthocyanidin dioxygenase (LDOX; ANS ) gene. PLoS ONE 10:e0142777 https://doi.org/10.1371/journal.pone.0142777
  4. Bonchev GN (2016) Useful parasites: the evolutionary biology and biotechnology applications of transposable elements. J Genet 95:1039-1052 https://doi.org/10.1007/s12041-016-0702-6
  5. Chung SJ, Lee G, Lee HJ, Kim J, Kim DS, Kang S (2010) Isolation of a leucoanthocyanidin dioxygenase (LDOX) gene from a spray-type chrysanthemum (Dendranthema $\times$ grandiflorum ) and its colored mutants. Korean J Hort Sci Technol 28:818-827
  6. Czemmel S, Heppel SC, Bogs J (2012) R2R3 MYB transcription factors: key regulators of the flavonoid biosynthetic pathway in grapevine. Protoplasma 249:S109-S118 https://doi.org/10.1007/s00709-012-0380-z
  7. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem Bull 19:11-15
  8. Ferrer JL, Austin MB, Stewart C Jr, Noel JP (2008) Structure and function of enzymes involved in the biosynthesis of phenylpropanoids. Plant Physiol Biochem 46:356-370 https://doi.org/10.1016/j.plaphy.2007.12.009
  9. Fini A, Brunetti C, Di Ferdinando M, Ferrini F, Tattini M (2011) Stress-induced flavonoid biosynthesis and the antioxidant machinery of plants. Plant Signal Behav 6:709-711 https://doi.org/10.4161/psb.6.5.15069
  10. Goodrich J, Carpenter R, Coen ES (1992) A common gene regulates pigmentation pattern in diverse plant species. Cell 68:955-964 https://doi.org/10.1016/0092-8674(92)90038-E
  11. Guo C, Yang J, Wei J, Li Y, Xu J, Jiang Y (2003) Antioxidant activities of peel, pulp and seed fractions of common fruits as determined by FRAP assay. Nutr Res 23:1719-1726 https://doi.org/10.1016/j.nutres.2003.08.005
  12. Holland D, Hatib K, Bar-Ya'akov I (2009) Pomegranate: botany, horticulture, breeding. Hortic Rev 35:127-191
  13. Holton TA, Cornish EC (1995) Genetics and biochemistry of anthocyanin biosynthesis. Plant Cell 7:1070-1083
  14. Kalaycioglu Z, Erim FB (2017) Total phenolic contents, antioxidant activities, and bioactive ingredients of juices from pomegranate cultivars worldwide. Food Chem 221:496-507 https://doi.org/10.1016/j.foodchem.2016.10.084
  15. Lee SS, Lee EM, An BC, Cho J, Kim T, Choi YO, Chung BY (2009) Isolation and characterization of flavanone 3-hydroxylase genes from a Korean raspberry. Korean J Hort Sci Technol 27:432-440
  16. Opara LU, Al-Ani MR, Al-Shuaibi YS (2009) Physico-chemical properties, vitamin C content, and antimicrobial properties of pomegranate fruit (Punica granatum L.). Food Bioprocess Technol 2:315-321 https://doi.org/10.1007/s11947-008-0095-5
  17. Panth N, Manandhar B, Paudel KR (2017) Anticancer activity of Punica granatum (Pomegranate): a review. Phytother Res 31:568-578 https://doi.org/10.1002/ptr.5784
  18. Passeri V, Koes R, Quattrocchio FM (2016) New challenges for the design of high value plant products: stabilization of anthocyanins in plant vacuoles. Front Plant Sci 7:153
  19. Petroni K, Tonelli C (2011) Recent advances on the regulation of anthocyanin synthesis in reproductive organs. Plant Sci 181:219-229 https://doi.org/10.1016/j.plantsci.2011.05.009
  20. Qin G, Xu C, Ming R, Tang H, Guyot R, Kramer EM, Hu Y, Yi X, Qi Y, Xu X, Gao Z, Pan H, Jian J, Tian Y, Yue Z, Xu Y (2017) The pomegranate (Punica granatum L.) genome and the genomics of punicalagin biosynthesis. Plant J 91:1108-1128 https://doi.org/10.1111/tpj.13625
  21. Quattrocchio F, Wing JF, Leppen HTC, Mol JN, Koes RE (1993) Regulatory genes controlling anthocyanin pigmentation are functionally conserved among plant species and have distinct sets of target genes. Plant Cell 5:1497-1512 https://doi.org/10.2307/3869734
  22. Ramsay NA, Glover BJ (2005) MYB-bHLH-WD40 protein complex and the evolution of cellular diversity. Trends Plant Sci 10:63-70
  23. Sarkhosh A, Zamani Z, Fatahi R, Ebadi A (2006) RAPD markers reveal polymorphism among some Iranian pomegranate (Punica granatum L.) genotypes. Sci Hortic 111:24-29 https://doi.org/10.1016/j.scienta.2006.07.033
  24. Shaygannia E, Bahmani M, Zamanzad B, Rafieian-Kopaei M (2016) A review study on Punica granatum L. J Evid Based Complement Altern Med 21:221-227 https://doi.org/10.1177/2156587215598039
  25. Shirley BW (1996) Flavonoid biosynthesis: 'new' functions for an 'old' pathway. Trends Plant Sci 1:377-382
  26. Spelt C, Quattrocchio F, Mol JN, Koes RE (2000) Anthocyanin1 of Petunia encodes a basic helix-loop-helix protein that directly activates transcription of structural anthocyanin genes. Plant Cell 12:1619-1631 https://doi.org/10.1105/tpc.12.9.1619
  27. Sreekumar S, Sithul H, Muraleedharan P, Azeez JM, Sreeharshan S (2014) Pomegranate fruit as a rich source of biologically active compounds. Biomed Res Int 2014:686921
  28. Veitch NC, Grayer RJ (2011) Flavonoids and their glycosides, including anthocyanins. Nat Prod Rep 28:1626-1695 https://doi.org/10.1039/c1np00044f
  29. Vogt T (2010) Phenylpropanoid biosynthesis. Mol Plant 3:2-20 https://doi.org/10.1093/mp/ssp106
  30. Wicker T, Sabot F, Hua-Van A, Bennetzen JL, Capy P, Chalhoub B, Flavell A, Leroy P, Morgante M, Panaud O, Paux E, SanMiguel P, Schulman AH (2007) A unified classification system for eukaryotic transposable elements. Nat Rev Genet 8:973-982 https://doi.org/10.1038/nrg2165
  31. Wu S, Tian L (2017) Diverse phytochemicals and bioactivities in the ancient fruit and modern functional food pomegranate (Punica granatum). Molecules 22:1606 https://doi.org/10.3390/molecules22101606
  32. Xu Z, Feng K, Que F, Wang F, Xiong A (2017) A MYB transcription factor, DcMYB6, is involved in regulating anthocyanin biosynthesis in purple carrot taproots. Sci Rep 7:45324 https://doi.org/10.1038/srep45324
  33. Yamazaki M, Makita Y, Springob K, Saito K (2003) Regulatory mechanisms for anthocyanin biosynthesis in chemotypes of Perilla frutescens var. crispa. Biochem Eng J 14:191-197 https://doi.org/10.1016/S1369-703X(02)00222-X
  34. Zhao X, Yuan Z, Feng L, Fang Y (2015) Cloning and expression of anthocyanin biosynthetic genes in red and white pomegranate. J Plant Res 128:687-696 https://doi.org/10.1007/s10265-015-0717-8