• 한국통신학회:학술대회논문집
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• 한국통신학회 1983년도 추계학술발표회논문집
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• pp.102-104
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• 1983

본 논문은 광대역에서 임의로 발생하는 TONE BURST 파형의 패턴을 여러파라미터로 분석하여 이를 잡음과 섞여있는 경우에 적용하였다. 그결과 특정의 REFERENCE 신호가 없는 경우에는 AUTOCORRELATION 향수가 TONEBURST파형분석에 최적 파라미터 임을 입증한다.

• Journal of Animal Science and Technology
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• 제57권5호
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• pp.18.1-18.8
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• 2015

Background: Quantitative real time reverse transcription PCR (qRT-PCR) is one of the most important techniques for gene-expression analysis in molecular based studies. Selecting a proper internal control gene for normalizing data is a crucial step in gene expression analysis via this method. The expression levels of reference genes should be remained constant among cells in different tissues. However, it seems that the location of cells in different tissues might influence their expression. The purpose of this study was to determine whether the source of mesenchymal stem cells (MSCs) has any effect on expression level of three common reference genes (GAPDH, ${\beta}$-actin and ${\beta}2$-microglobulin) in equine marrow- and adipose-derived undifferentiated MSCs and consequently their reliability for comparative qRT-PCR. Materials and methods: Adipose tissue (AT) and bone marrow (BM) samples were harvested from 3 mares. MSCs were isolated and cultured until passage 3 (P3). Total RNA of P3 cells was extracted for cDNA synthesis. The generated cDNAs were analyzed by quantitative real-time PCR. The PCR reactions were ended with a melting curve analysis to verify the specificity of amplicon. Results: The expression levels of GAPDH were significantly different between AT- and BM-derived MSCs (p < 0.05). Differences in expression level of ${\beta}$-actin (P < 0.001) and B2M (P < 0.006.) between MSCs derived from AT and BM were substantially higher than GAPDH. In addition, the fold change in expression levels of GAPDH, ${\beta}$-actin and B2M in AT-derived MSCs compared to BM-derived MSCs were 2.38, 6.76 and 7.76, respectively. Conclusion: This study demonstrated that GAPDH and especially ${\beta}$-actin and B2M express in different levels in equine AT- and BM-derived MSCs. Thus they cannot be considered as reliable reference genes for comparative quantitative gene expression analysis in MSCs derived from equine bone marrow and adipose tissue.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.4102-4102
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• 2001

In multivariate analysis, absorbance spectrum is measured over a band of wavelengths. One does not often pay attention to the size of this wavelength band. However, it is desirable that spectrum is measured at only necessary wavelengths as long as the acceptable accuracy of prediction can be met. In this paper, the method of selecting an optimal band of wavelengths based on the loading vector analysis was proposed and applied for determining total protein in human serum using near-infrared transmission spectroscopy and PLSR. Loading vectors in the full spectrum PLSR were used as reference in selecting wavelengths, but only the first loading vector was used since it explains the spectrum best. Absorbance spectra of sera from 97 outpatients were measured at 1530∼1850 nm with an interval of 2 nm. Total protein concentrations of sera were ranged from 5.1 to 7.7 g/㎗. Spectra were measured by Cary 5E spectrophotometer (Varian, Australia). Serum in the 5 mm-pathlength cuvette was put in the sample beam and air in the reference beam. Full spectrum PLSR was applied to determine total protein from sera. Next, the wavelength region of 1672∼1754 nm was selected based on the first loading vector analysis. Standard Error of Cross Validation (SECV) of full spectrum (1530∼l850 nm) PLSR and selected wavelength PLSR (1672∼1754 nm) was respectively 0.28 and 0.27 g/㎗. The prediction accuracy between the two bands was equal. Wavelength selection based on loading vector in PLSR seemed to be simple and robust in comparison to other methods based on correlation plot, regression vector and genetic algorithm. As a reference of wavelength selection for PLSR, the loading vector has the advantage over the correlation plot since the former is based on multivariate model whereas the latter, on univariate model. Wavelength selection by the first loading vector analysis requires shorter computation time than that by genetic algorithm and needs not smoothing.

• Nuclear Engineering and Technology
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• 제48권3호
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• pp.624-634
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• 2016

Nuclear criticality safety analyses (NCSAs) considering burnup credit were performed for the GBC-32 cask. The used nuclear fuel assemblies (UNFAs) discharged from Hanbit Nuclear Power Plant Unit 3 Cycle 6 were loaded into the cask. Their axial burnup distributions and average discharge burnups were evaluated using the DeCART and Multi-purpose Analyzer for Static and Transient Effects of Reactors (MASTER) codes, and NCSAs were performed using SCALE 6.1/STandardized Analysis of Reactivity for Burnup Credit using SCALE (STARBUCS) and Monte Carlo N-Particle transport code, version 6 (MCNP 6). The axial burnup distributions were determined for 20 UNFAs with various initial enrichments and burnups, which were applied to the criticality analysis for the cask system. The UNFAs for 20- and 30-year cooling times were assumed to be stored in the cask. The criticality analyses indicated that $k_{eff}$ values for UNFAs with nonuniform axial burnup distributions were larger than those with a uniform distribution, that is, the end effects were positive but much smaller than those with the reference distribution. The axial burnup distributions for 20 UNFAs had shapes that were more symmetrical with a less steep gradient in the upper region than the reference ones of the United States Department of Energy. These differences in the axial burnup distributions resulted in a significant reduction in end effects compared with the reference.

• International Journal of Industrial Entomology and Biomaterials
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• 제33권2호
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• pp.121-131
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• 2016

Ten microsporidian isolates from Samia cynthia ricini, and Antheraea assamensis in India along with a Nosema reference strain (NIK-1s_mys) from B. mori India were characterised morphologically and molecular based tools. The test isolates observed elongated oval in shape while reference strain was oval and ranging from 3.80 to 4.90 m in length and 2.60 to 3.05 m in width. The ribosomal DNA region 'IGS' of test isolates assessed by PCR amplification, followed by cloning and sequencing. IGS sequence and phylogenetic analysis of test microsporidian isolates showed very close relationship with three Nosema references species: N. philosamia, N. antheraea isolated from Philosamia cynthia ricini and Antheraea perny in China respectively and N. disstriae from Malacosma disstriae in Canada. The clustering pattern of dendogram reveals all test isolates appear distinct from Nosema std. (NIK-1s_mys) India used as reference strain in the study. The result suggests IGS indeed a suitable and highly applicable molecular tool for identifying and characterise the microsporidian isolates in similar population.

• 한국지반공학회지:지반
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• 제6권4호
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• pp.33-42
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• 1990

정적관입저항 또는 선단지지말뚝의 선단지지력을 해석함에 있어서는 유효수직응력이 기준음력으로 사용되어 왔다. 그러나 정적관입저항은 유효수직응력보다 원위치 유효수평응력에 관계된다는 것이 많은 실험연구결과로부터 보고 되었다. 이와같은 문제점을 규명하기 위하여 기 보고된 실험결과를 다시 분석하였으며 실험실에서의 관입시험이 실시되었다. 연구결과 정적관입저항은 유효수직 응력과 유효수평응력 모두에 영향을 받는 것으로 나타나고 있으며 해석시에는 평균주응력이 기준응력으로 사용되어야 함을 결론 지을 수 있었다.

• 대한기계학회논문집A
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• 제26권3호
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• pp.505-513
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• 2002

During heatup and cooldown of pressurized water reactor, thermal stress was generated in the reactor pressure vessel (RPV) because of the temperature gradient. To prevent potential failure of RPV, pressure was required to be maintained below the P-T limit curves. In this paper, several methods for constructing the P-T limit curves including the ASME Sec. XI, App. G method were explained and the results were compared. Then, the effects of the various parameters such as flaw size, flaw orientation, cooldown rate, existence of chad, and reference fracture toughness, were evaluated. It was found that the current ASME Sec. XI App. G method resulted in the most conservative P-T limit curve. As the more accurate fracture mechanics analysis results were used, some of the conservatism can be removed. Among the parameters analysed, reference flaw orientation and reference fracture toughness curve had the greatest effect on the resulting P-T limit curves.

• 대한기계학회논문집A
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• 제26권6호
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• pp.1127-1134
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• 2002

The present paper provides an engineering J estimation equation for surface cracked plates under combined bending and tension. The proposed equation is based on the reference stress approach, and the most relevant normalising loads to define the reference stress for accurate J estimations are given for surface cracked plates under combined bending and tension. Comparisons with J results from extensive 3-D FE analyses, covering a wide range of crack geometry, plate geometry and loading combination, show overall good agreement not only at the deepest point but also at arbitrary points along the crack front. for pure tension, agreement between the estimated J and the FE results is excellent, even at the surface point. On the other hand, for pure bending and combined bending and tension, the estimated J values become less accurate for locations close to the surface point. Thus the results in this paper will be useful to assess short-term fracture or low cycle fatigue of surface defects in plates under combined bending and tension.

• 한국정밀공학회지
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• 제18권6호
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• pp.67-73
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• 2001

Three dimensional, elastic-plastic finite element(FE) analyses for circumferential through-wall cracked pipes under combined tension and bending are performed using actual tensile data of stainless steel, for two purposes. The first one is to validate the recently-proposed enhanced reference stress (ERS) method to estimate the J-integral and COD for circumferential through-wall cracked pipes under combined tension and bending. The second one is to compare those results with the GE/EPRI estimations. The FE results of the J-integral and the COD, resulting from six cases of proportional and non-proportional combined tension and bending, compare very well with those estimated from the proposed method. Excellent agreements of the proposed method with the detailed FE results provide sufficient confidence in the use of the proposed method to the Leak-Before-Break(LBB) analysis of through-wall cracked pipes under combined tension and bending.

• 한국비블리아학회지
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• 제24권4호
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• pp.23-51
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• 2013

이 연구는 정부기능분류체계가 기록분류에 어떻게 적용되고 있는지, 그 가능성과 한계는 무엇인지 밝히기 위한 것이다. 자료 수집을 위해 6개 중앙행정기관의 기록관리전문직 6명을 대상으로 3회에 걸친 집단면담을 실시하였다. 우선 공공기록물관리법률 분석을 통해 기록물분류제도를 살펴본 후, 정부기능분류체계를 기록분류에 적용함으로써 얻을 수 있는 편익의 유형을 조사하였다. 면담 자료를 토대로 단위과제를 활용한 기록물철 분류의 실태와 문제점을 구조 및 운용 측면에서 분석하였다.