• Proceedings of the International Microelectronics And Packaging Society Conference
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• 2003.11a
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• pp.151-155
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• 2003

The requirements for harsh environment electronic controllers in automotive applications have been steadily becoming more and more stringent. Electronic substrate technologists have been responding to this challenge effectively in an effort to meet the performance, reliability and cost requirements. An effect of the plasma cleaning at the alumina substrate and the IMC layer between $Sn-37wt\%Pb$ solder and Ag-Pd thick film conductor after reflow soldering has been studied. Organic residual carbon layer was removed by the substrate plasma cleaning. So the interfacial adhesive strength was enhanced. As a result of AFM measurement, Ag-Pd conductor pad roughness were increased from 304nm to 330nm. $Cu_6Sn_5$ formed during initial ref]ow process at the interface between TiWN/Cu UBM and solder grew by the succeeding reflow process so the grains had a large diameter and dense interval. A cellular-shaped $Ag_3Sn$ was observed at the interface between Ag-Pd conductor pad and solder. The diameters of the $Ag_3Sn$ grains ranged from about $0.1\~0.6{\mu}m$. And a needle-shaped $Ag_3Sn$ was also observed at the inside of the solder.

• Proceedings of the KIPE Conference
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• 2018.07a
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• pp.132-134
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• 2018

본 논문은 태양광발전 시스템에서 P&O 방식 기반의 제어 기법인 duty 제어와 전류 제어 방식의 최대전력점 추종특성을 동일모드 조건에서 비교하였으며, 시뮬레이션 및 실험을 통해 검증하였다. Duty 제어 방식은 P-V 특성곡선을 이용하여 전력과 전압의 변화량을 통해 duty 증감을 결정함으로써 동작점을 결정한다. 이와 달리, 전류 제어 방식은 P-I 특성곡선을 이용하여 전력과 전류의 변화량을 통해 전류 지령치를 생성하고 비례-적분제어기를 통해 전류제어를 수행하여 동작점을 결정한다. 두 가지 제어 방식의 정확한 비교를 위해 등가의 ${\Delta}duty$ 및 ${\Delta}I_{ref}$의 값을 선정하여 모드 변화에 따른 ${\Delta}V$의 변화를 동일하게 맞추었다. DC - DC 부스트 컨버터를 이용하여 시뮬레이션과 실험에 두 제어 방식의 태양광발전 시스템을 구성하여 태양광 전압과 전류, 에너지 측면에서 특성을 비교하였다. 최대 전력점이 1.7kW인 특성곡선 조건 하에서, 시뮬레이션과 실험의 결과로 두 방식은 동일 MPPT 모드 시 동일한 동작점에 존재하며, 같은 값의 전력을 출력하여 동일한 성능의 효율을 보임을 검증하였다.

• Journal of the Korean Institute of Landscape Architecture
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• v.23 no.2
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• pp.93-103
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• 1995

The changes of rural landscape are currently spread everywhere and are rapidily being processed both in quality and in quantities of changes From such aspects, it is urgent to control the land scape change, and is required to know what is valued or preferred in rural landscape, This study was conducted to investigate preferences and differences of the responses between urban and rural residents to rural scenery. As a method, color-slide surrogates for rural landscape settings are shown to urban and rural residents, For a given 52 scenes of rural landscapes the respondents judged the familiarity, orality, and preference along a ten point scale. The following facts are found: 1) Refponses to certain types of landscape settings are influenced by the place of residence of the respondents. The urban residents highly rated natural components of the rural landscape. In contrast rural residents preferred the scenes with visible sign of development 2) The relations among the estimation of preference, familiarity and rurality in rural landscape were deeply correlated. When the rural lands(:ape is adequately ref resented as 'familiarity' or 'rurality' well the landscape is more preferred 3) It is found what is valued or preferred in rural landscape : Readjusted agricultural land, big tree, grassland hi dominated visually were preferred in rural landscape. The implication of this study is that successful planning of development or management in rural area will occur only when these different needs and values are acknowledged and accommodated.

• International Journal of Naval Architecture and Ocean Engineering
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• v.7 no.6
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• pp.939-950
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• 2015

Wave-induced Longshore Sediment Transport (LST) play an important role in the dynamics of the Dhanushkodi sandspit located southeast of Rameshwaram. The LST along the Dhanushkodi coast is studied based on data collected simultaneously in Gulf of Mannar (GoM) and Palk Bay (PB) using directional waverider buoys. The numerical model REF/DIF1 was used to calculate the nearshore waves and the LST rate was estimated using three different formulae. The model validation was done based on the measured nearshore waves using InterOcean S4DW. Numerical model LITPACK was also used for simulating non-cohesive sediment transport and the LITLINE module was used to study the shoreline evolution over 5 years. Low net annual LST along PB (${\sim}0.01{\times}10^6m^3$) compared to the GoM region ($0.3{\times}10^6m^3$) were due to the weak waves. Accretion in the region led to growth of the Dhanushkodi sandspit by 65 m during the period 2010-2015.

• Journal of the Korean Society of Industry Convergence
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• v.24 no.5
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• pp.635-639
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• 2021

As times goes by, a ton of electric devices have been developing. Nowadays, there are many personal electric goods that are connected each other and have important private information such as identification, account number, passwords, and so on. As many people own at least one electric device, security of the electric devices became significant. To prevent leakage of the information, study of Chaotic TRNG, "Chaotic True Random Number Generator", protecting the information by generating random numbers that are not able to be expected, is essential. In this paper, A chaotic TRNG is introduced is simulated. The proposed Chaotic TRNG is simulated with Virtuoso &, a circuit design program of Cadence that is a software company. For simulating the mentioned Chaotic TRNG, setting values, 0V low and 3V high on Vpulse, 1.2V on V-ref, 3.3V on VDD, and 0V on VSS, are used.

• Genomics & Informatics
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• v.21 no.4
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• pp.49.1-49.11
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• 2023

Recent advances in sequencing technologies and platforms have enabled to generate metagenomics sequences using different sequencing platforms. In this study, we analyzed and compared shotgun metagenomic sequences generated by HiSeq3000 and BGISEQ-500 platforms from 12 sediment samples collected across the Norwegian coast. Metagenomics DNA sequences were normalized to an equal number of bases for both platforms and further evaluated by using different taxonomic classifiers, reference databases, and assemblers. Normalized BGISEQ-500 sequences retained more reads and base counts after preprocessing, while a slightly higher fraction of HiSeq3000 sequences were taxonomically classified. Kaiju classified a higher percentage of reads relative to Kraken2 for both platforms, and comparison of reference database for taxonomic classification showed that MAR database outperformed RefSeq. Assembly using MEGAHIT produced longer assemblies and higher total contigs count in majority of HiSeq3000 samples than using metaSPAdes, but the assembly statistics notably improved with unprocessed or normalized reads. Our results indicate that both platforms perform comparably in terms of the percentage of taxonomically classified reads and assembled contig statistics for metagenomics samples. This study provides valuable insights for researchers in selecting an appropriate sequencing platform and bioinformatics pipeline for their metagenomics studies.

• Genomics & Informatics
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• v.9 no.3
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• pp.102-113
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• 2011

C-terminal SRC kinase (CSK) is a ubiquitously expressed, cytosolic enzyme that phosphorylates and inactivates several SRC family protein tyrosine kinases. Recent genomewide association studies have implicated CSK in the regulation of blood pressure. The current study aim is to determine the blood pressure association of the genes regulated by CSK down-regulation. The CSK mRNA expression was downregulated in vascular smooth muscle cells using small interfering RNA (siRNA). CSK mRNA levels fell by 90% in cells that were treated with CSK siRNA; the RNA from these cells was examined by microarray using the Illumina HumanRef-8 v3 platform, which comprises 24,526 reference mRNA probes. On treatment with CSK siRNA, 19 genes were downregulated by more than 2-fold and 13 genes were upregulated by more than 2-fold. Three (CANX, SLC30A7, and HMOX1) of them revealed more than 3 fold differential expression. Interestingly, the HMOX1 SNPs were associated with diastolic blood pressure in the 7551 Koreans using Korea Association REsource data, and the result was supported by the other reports that HMOX1 linked to blood vessel maintenance. Among the remaining 29 differentially expressed genes, seven (SSBP1, CDH2, YWHAE, ME2, PFTK1, G3BP2, and TUFT1) revealed association with both systolic and diastolic blood pressures. The CDH2 gene was linked to blood pressures. Conclusively, we identified 32 differentially expressed genes which were regulated by CSK reduction, and two (HOMX1 and CDH2) of them might influence the blood pressure regulation through CSK pathway.

• Journal of Life Science
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• v.23 no.1
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• pp.116-124
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• 2013

The aim was to examine resistance exercise-related genes after 8 weeks of resistance training. Thirty-two male Sprague-Dawley rats were divided into four groups: 4 weeks sedentary (4 wks CON, n=8), 8 weeks sedentary (8 wks CON, n=8), 4 weeks exercise training (4 wks REG, n=8), and 8 weeks exercise training (8 wks REG, n=8). The rats were trained to climb a 1-m vertical incline (85-degree), with weights secured to their tails. They climbed 10 times, 3 days per week, for 8 consecutive weeks. Skeletal muscle was taken from the flexor halucis longus after the exercise training. After separating the total RNA, large-scale gene expression was investigated by beadarray (Illumina RatRef-12 Expression BeadChip) analysis, and qPCR was used to inspect the beadarray data and to analyze the RNA quantitatively. The detection p-value for the genes was p<0.01, the M-value {M=$log_2$(condition)-$log_2$(reference)} was >1.0, and the DiffScore was >20. In total, the expression of 30 genes significantly increased 4 weeks after the exercise training, and the expression of six genes decreased. At 8 weeks, the expression of five genes significantly increased and that of 12 decreased. Several genes are potentially involved in resistance exercise and muscle hypertrophy, including 1) regulation of cell growth (IGFBP1, PLA2G2A, OKL38); 2) myogenesis (CSRP3); 3) tissue regeneration and muscle development (MUSTN1, MYBPH); 4) hypertrophy (CYR61, ATF3, NR4A3); and 5) glucose metabolism (G6PC, PCK1). These results may help to explain previously reported physiological changes of the skeletal muscle and suggest new avenues for further investigation.

• Microbiology and Biotechnology Letters
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• v.50 no.2
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• pp.283-292
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• 2022

Colibactins (clb) and Cytotoxic Necrotizing Factors (cnf) are virulence factors that impact cell cycle through cellular differentiation, proliferation, and apoptosis. Urinary tract infections (UTIs) are the most common among type of infection among outpatients, with a lifetime incidence of about 60-65% in adult females. Here, we sought to isolate uropathogenic Escherichia coli (UPCE) from urine specimens and investigates the prevalence of clb A, B and cnf 1, 2 genes among these isolates. A total of 110 E. coli isolates were collected from patients with UTIs. All the isolates were examined for their hemolytic activity and only 46 isolates showed a halo zone of hemolysis on blood agar. The collected UPEC isolates were screened for the existence of clb A, B and cnf genes. The results revealed that out of 110 isolates, 28 harbored the clbA gene, 40 harbored clb B, and 24 isolates harboured cnf1. 13 isolates harbored clbA, clbB, and cnf1 genes, while no cnf2 gene was detected among isolates. The molecular detection revealed that 8 out of 28 hemolytic isolates carrying the clbA, 11 out of 40 were carrying clbB, 1 out of 24 were carrying cnf 1, and 5 out of 9 carrying clbA+clbB. Furthermore, 7 out of 13 isolates were hemolytic and carrying clbA, clbB, and cnf1 genes. Finally, we investigated the cytotoxicity of E. coli harboring clb and cnf genes, eukaryotic REF cells were exposed to E. coli producing colibactin, which induces DNA damage and leads to cell cycle arrest, senescence and death.

• Toxicological Research
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• v.26 no.1
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• pp.21-28
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• 2010

As the frequency and the intensity of so called Asian dust (AD) events have increased, public concerns about the adverse health effects has spiked sharply over the last two decades. Despite the recent reports on the correlation between AD events and the risk for cardiovascular and respiratory disease, the nature of the toxicity and the degree of the risk are yet largely unknown. In the present study, we investigated the effects of the dichloromethane extract of AD (AD-X) and that of urban dust (NAD-X) collected during a non-AD period on gene expression in HL-60 cells using Illumina Sentrix HumanRef-8 Expression BeadChips. Global changes in gene expression were analyzed after 24 h of incubation with 50 or 100 ${\mu}g$/ml AD-X and NAD-X. By one-way analysis of variance (p < 0.05) and Benjamini-Hochberg multiple testing correction for false discovery rate of the results, 573 and 297 genes were identified as AD-X- and NAD-X-responsive, respectively. The genes were classified into three groups by Venn diagram analysis of their expression profile, i.e., 290 AD-X-specific, 14 NAD-X-specific, and 283 overlapping genes. Quantitative realtime PCR confirmed the changes in the expression levels of the selected genes. The expression patterns of five genes, namely SORL1, RABEPK, DDIT4, AZU1, and NUDT1 differed significantly between the two groups. Following rigorous validation process, these genes may provide information in developing biomarker for AD exposure.