• 한국동물번식학회:학술대회논문집
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• 한국동물번식학회 2003년도 학술발표대회 발표논문초록집
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• pp.78-78
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• 2003

Embryonic stem (ES) cells, derived from preimplantation embryo, are able to differentiate into various types of cells consisting the whole body, or pluripotency. In contrast, terminally differentiated cells do not usually alter their nature but frequently die or transform if they are exposed to inappropriate external stimulations. In addition to the plasticity, ES cells are expected to be different from terminally differentiated cells in very many ways, such as patterns of gene expressions, ability and response of the cells in confronting environmental stimulations, metabolism, and growth rate. As a model system to differentiate these two types of cells, human ES cells (MB03) and terminally differentiated cells (HeLa), we examined the ability of these two types of cells in confronting a severe oxidative insult, that is $H_2O$$_2$. Approximately 1$\times$10$^4$ cells were plated in 96 well plate and serum starved for overnight. The conditioned cells were exposed to a various concentration of $H_2O$$_2$ fur 24 hrs and loaded with neutral red (50$\mu\textrm{g}$/ml) for 4 hrs, washed with PBS for 2 min three times, and entrapped dye was dissolved out using acetic ethanol. Cytotoxicity was determined by reading the amount of dye in the medium using microplate reader. equipped with 575 nm filter. Relative amount of the dye entrapped within MB03 or HeLa were not significantly different when cells were exposed up to 0.4 mM $H_2O$$_2$. However, this sharply decreased down to 0.12% in HeLa cells when the cells were exposed to 0.8 mM $H_2O$$_2$, while it was approximately 54% in MB03 suggesting that this concentration of $H_2O$$_2$ is the defensive threshold for HeLa cells. The resistance to oxidative stimulation reversed, however, when cells were co-treated with BSO (L-buthionine- 〔S, R〕-sulfoximine) which chelates intracellular GSH. This result suggests that cellular GSH is the major defensive mechanism of human ES cells. Induction of enzymes involved in GSH metabolism and type of cell death is currently being studied.

• Journal of Astronomy and Space Sciences
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• 제25권3호
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• pp.249-254
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• 2008

We report the distance modulus of nearby dwarf irregular galaxy NGC 6822 estimated from the so-called Tip of Red-giant Branch (TRGB) method. To detect the apparent magnitudes of the TRGB we use the color-magnitude diagrams (CMDs) and luminosity functions (LFs) in the near-infrared JHK bands. Foreground stars, main-sequence stars, and supergiant stars have been classified on the (g - K, g) plane and removed on the near-infrared CMDs, from which only RGB and AGB stars are remained on the CMDs and LFs. By applying the Savitzky-Golay filter to the obtained LFs and detecting the peak in the second derivative of the observed LFs, we determined the apparent magnitudes of the TRGB. Theoretical absolute magnitudes of the TRGB are estimated from Yonsei-Yale isochrones with the age of 12Gyr and the metallicity range of -2.0 <[Fe/H]< -0.5. The derived values of distance modulus to NGC 6822 are (m - M) = $23.35{\pm}0.26$, $23.20{\pm}0.42$, and $23.27{\pm}0.50$ for J, H, and K bands, respectively. Distance modulus in bolometric magnitude is also derived as (m - M) = $23.41{\pm}0.17$. We compare the derived values of the TRGB distance modulus to NGC 6822 in the near-infrared bands with the previous results in other bands.

• 천문학회지
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• 제40권3호
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• pp.67-82
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• 2007

The basic building block of solar filaments/prominences is thin threads of cool plasma. We have studied the spectral properties of velocity threads, clusters of thinner density threads moving together, by analyzing a sequence of $H{\alpha}$ images of a quiescent filament. The images were taken at Big Bear Solar Observatory with the Lyot filter being successively tuned to wavelengths of -0.6, -0.3, 0.0, +0.3, and +0.6 ${\AA}$ from the centerline. The spectra of contrast constructed from the image data at each spatial point were analyzed using cloud models with a single velocity component, or three velocity components. As a result, we have identified a couple of velocity threads that are characterized by a narrow Doppler width($\Delta\lambda_D=0.27{\AA}$), a moderate value of optical thickness at the $H{\alpha}$ absorption peak($\tau_0=0.3$), and a spatial width(FWHM) of about 1". It has also been inferred that there exist 4-6 velocity threads along the line of sight at each spatial resolution element inside the filament. In about half of the threads, matter moves fast with a line-of-sight speed of $15{\pm}3km\;s^{-1}$, but in the other half it is either at rest or slowly moving with a line-of-sight velocity of $0{\pm}3km\;s^{-1}$. It is found that a statistical balance approximately holds between the numbers of blue-shifted threads and red-shifted threads, and any imbalance between the two numbers is responsible for the non-zero line-of-sight velocity determined using a single-component model fit. Our results support the existence not only of high speed counter-streaming flows, but also of a significant amount of cool matter either being at rest or moving slowly inside the filament.

• 한국연초학회지
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• 제32권1호
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• pp.19-27
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• 2010

The aim of this study is to compare the sensitivity of both two NCI-H292 and A549 cell types to acute inflammatory responses induced by cigarette smoke. For this, we treated two kinds of smoke fractions derived from 2R4F reference cigarettes: total particulate matter(TPM) collected onto a Cambridge filter pad and gas/vapor phase(GVP) prepared by bubbling through in buffer solution. When we measured cellular cytotoxicity by neutral red uptake assay after treatment for 24 hours, TPM and GVP induced cytotoxic effect in a dose-dependent manner in the range of 10-$100{\mu}g$/mL and 60-$300 {\mu}g$/mL., respectively, in both cell types without any cellular difference. Additionally, when we examined acute inflammatory responses by analyzing cytokines secreted into culture media including tumor necrosis factor-$\alpha$ (TNF-$\alpha$), interleukin-8(IL-8), and transforming growth factor-$\alpha$(TGF-$\alpha$) as well as matrix metalloproteinase-1(MMP-1), the treatment with smoke fractions increased those marker proteins in a dose-dependent manner in NCI-H292. Meanwhile, in A549 cells only MMP-1 was observed to be increased in a dose-dependent fashion. Collectively, our data indicate that NCI-H292 cell type is more sensitive to cigarette smoke-induced inflammatory response than A549 cells. This suggests that NCI-H292 could be useful as an in vitro evaluation tool to assess harmful effects of cigarette smoke.

• 전자공학회논문지SC
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• 제44권2호
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• pp.10-17
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• 2007

의료용 초음파 시스템으로 혈류 속도를 측정할 때, 순수한 혈류 신호의 검출이 필요하다. 초음파 트랜스듀서를 통해 들어오는 반사 신호는 체세포 조직(tissue), 혈관 벽(blood wall), 적혈구(red blood cell), 잡음(noise) 등이 혼합된 신호이다. 혼합된 신호에서 체세포 조직과 혈관 벽 신호를 클러터(clutter)라고 한다. 본 논문에서는 ICA(independent component analysis)를 적용하여 클러터 신호와 잡음을 효과적으로 제거하는 방법을 제시하였다. Field II 초음파 시뮬레이션 프로그램을 이용하여 초음파 반사 신호를 생성하고, ICA를 사용하여 각 독립 신호들을 분리, 클러터 신호를 제거하여 혈류 신호를 추출했다. 추출전 혈류신호를 2D 자기상관(autocorrelation) 방법으로 혈류 속도를 측정했다. 그리고 PCA(principal component analysis)방법을 적용한 고유 필터(autocorrelation) 방법으로 클러터를 제거한 결과와 비교하였다. 그 결과 잡음 환경에서의 혈류 속도 측정에 ICA 방법이 우수한 적용 결과를 보였다.

• 한국습지학회지
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• 제9권2호
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• pp.71-84
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• 2007

마산만은 한국의 대표적인 반 폐쇄성 내만으로 유속이 느리며, 해수의 순환이 제한되어 입자물질의 침강이 주로 일어나고 있다. 특히, 육상기원 오염물질 유입은 환경오염문제를 일으켜, 부영양화, 적조, 저층 빈산소 현상 등의 문제가 빈번히 발생하고 있다. 본 연구에서는 생태계 모델을 이용하여, 이매패류를 포함하는 생태계 시스템에서 물질순환 과정의 계산을 수행하였으며, 여과식자인 이매패류에 의한 COD와 식물플랑크톤 같은 입자유기물질의 제거 효과도 계산하였다. 또한, 이매패류 양식장의 입식위치와 입식량을 산정하여, 생물학적 수질관리에 적용하고자 하였다. 모델결과에서, 최적 입식위치는 해양 물리적 과정을 참고로 하여 식물플랑크톤의 집적이 일어나는 곳을 선정하였으며, 최적 입식밀도는 35 개체/$m^{-3}$였고, 최적 입식 면적은 약 500 ha로 나타났다. 이매패류가 최적의 성장을 보인다고 가정하면, 육상부하의 삭감에 관한 노력 없이도 수중의 COD 농도의 18%의 개선효과가 나타났다.

• 한국산업융합학회 논문집
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• 제8권1호
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• pp.47-55
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• 2005

Infrared heating has been traditionally used in industrial applications for processes such as dehydration of food industrial. This heating method involves the application of radiation in the wavelength range of 2 to 50 micrometers. In this work, simultaneous heat balance equations were developed to simulate the infrared radiation heating of agricultural products. The equations assume that moisture diffuses to the outer boundaries of the material in liquid form and evaporation occurs at the surface of the agricultural products. Energy for moisture evaporation is supplied by the infrared radiant energy. The optimum temperature and drying time for the best drying conditions of changing the red peppers with the moisture content of 18% and the restore rate of 80~85% are $80^{\circ}C$ and 44 hours. The performance of radiation tubes coating with the radiation paint developed in this research has the energy of $2.27{\times}103W/m^2{\mu}m$, $150^{\circ}C$ within the scope of radiation wave length of $2{\sim}30{\mu}m$ and has the radiation 0.92~0.93, which is superior to the general radiation tubes. The extinction coefficient according to the band pass filter using the 4 flux theory ha higher dependability on wave length, accounting for $2{\sim}17{\mu}m$ and $5{\times}105{\sim}106m-1$. A comparison between the theoretical energy transfer whose figures are interpreted according to 4 flux theory and the experimental energy transfer of far infrared dryer leads to the findings of the agreement less than 5%.

• Fisheries and Aquatic Sciences
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• 제9권4호
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• pp.153-159
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• 2006

The ark shell (or 'blood clam') Scapharca broughtonii is a filter-feeding bivalve that has red blood and inhabits waters approximately 10m in depth off the southern coast of South Korea. This study was part of a larger research project investigating the causes of death and restoration of shellfish resources, which are important aquaculture products in South Korea. We examined physiological responses related to survival, respiration, excretion, and amino acid changes as a result of changes in salinity. The 9-day median lethal salinity ($LS_{50}$) was 16.5 psu with confidence limits of 14.9-18.1 psu. At $25^{\circ}C$, the oxygen consumption and ammonia-nitrogen excretion rates were increased with decreases in salinity. Although the osmolality of individuals was acclimated within 2 h at 26.4 psu and 12 h at 19.8 psu, it took more than 5 days at a salinity of 13.2 psu, whereas no individuals acclimated and all died at a salinity of 6.6 psu. Of the amino acids present in the blood, taurine and alanine increased in response to decreased salinity. Tissues of the gill and the mid-gut gland were affected by decreasing salinity. These data will provide important fundamental information for examining the causes of mass mortality of shellfish in the summer.

• 농업과학연구
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• 제40권2호
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• pp.163-167
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• 2013

Objective of the study was to compare reflectance spectrum in the blade and the vein parts of cabbage and kale leaves. A total 6 cabbage and kale leaves were taken from a plant factory in Chungnam National University, Korea. Spectra data were collected with a UV/VIS/NIR spectrometer (model: USB2000, Ocean Optics, FL, USA) in the wavelength region of 190 - 1130 nm. Median filter smoothing method was selected to preprocess the obtained spectra data. We computed reflectance difference by subtraction of averaged spectrum from individual spectrum. To estimate correlation at different parts of cabbage and kale leaves, cross - correlation method was used. Differences between cabbage and kale leaves are clearly manifested in the green, red and near - infrared ranges. The percent reflectance of cabbage leaves in the NIR wavelength band was higher than that of kale leaves. Reflectance in the blade part was higher than in the vein part by 18%. Reflectance difference in the different parts of cabbage and kale leaves were clear in all of the wavelength bands. Standard deviation of reflectance difference in the vein part was greater for kale, while the value in the blade part was greater for cabbage leaves. Standard deviation of cross - correlation increased from 0.092 in the first sensor (UV/VIS) and 0.007 in the second sensor (NIR) to 0.099 and 0.015, respectively.

• 천문학논총
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• 제30권2호
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• pp.405-407
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• 2015

We describe a survey of quasars in the early universe, beyond z ~ 5, which is one of the main science goals of the Infrared Medium-deep Survey (IMS) conducted by the Center for the Exploration of the Origin of the Universe (CEOU). We use multi-wavelength archival data from SDSS, CFHTLS, UKIDSS, WISE, and SWIRE, which provide deep images over wide areas suitable for searching for high redshift quasars. In addition, we carried out a J-band imaging survey at the United Kingdom InfraRed Telescope with a depth of ~23 AB mag and survey area of ${\sim}120deg^2$, which makes IMS a suitable survey for finding faint, high redshift quasars at z ~ 7. In addition, for the quasar candidates at z ~ 5.5, we are conducting observations with the Camera for QUasars in EArly uNiverse (CQUEAN) on the 2.1m telescope at McDonald Observatory, which has a custom-designed filter set installed to enhance the efficiency of selecting robust quasar candidate samples in this redshift range. We used various color-color diagrams suitable for the specific redshift ranges, which can reduce contaminating sources such as M/L/T dwarfs, low redshift galaxies, and instrumental defects. The high redshift quasars we are confirming can provide us with clues to the growth of supermassive black holes since z ~ 7. By expanding the quasar sample at 5 < z < 7, the final stage of the hydrogen reionization in the intergalactic medium (IGM) can also be fully understood. Moreover, we can make useful constraints on the quasar luminosity function to study the contribution of quasars to the IGM reionization.