• Journal of Haehwa Medicine
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• v.19 no.2
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• pp.145-151
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• 2011

Backgrounds: Multidisciplinary approaches including surgery, chemotherapy, and radiation therapy are currently being performed to target various cancers in Western Medicine. However, some cancers still remain difficult to battle, which has long attracted many scientists for the discovery of new agents to fight cancers. Ginseng is one of the herbs used in Oriental Medicine including Korea, China and Japan. We have further investigated ginseng for its anticancer effect. Objective: This is a comprehensive review summary of anticancer effect of ginseng and ginsenoids as a possible agent for future cancer treatment. Methods: Data were retrieved from two web sites; www.pubmed.com and www.riss.kr, and authorized texts concerning anticancer effects of ginseng. From collected data, information on anticancer effect of ginseng was thoroughly sorted, restructured, then assessed. Results: Panax Ginseng C.A. Meyer belongs to Araliaceae Panax family, a perennial prairie plant with its root known as Ginseng Radix. Ginseng induces anticancer effect through cell cycle arrest, acceleration of apoptosis, anti-angiogenesis, and suppression of metastasis. Anticancer effect of ginseng may be due to single compound or multi-compound actions. Many studies report involvement of immune mechanisms of cytokines, Natural Killer (NK) cells, macrophages and some antibodies in enhancing anticancer effect of ginseng. In near future, possibility of applying these mechanisms into clinical trials is convinced. There were some important findings on saponin in ginsenoids in reviewing for this article; First, eradication of metastatic tumors were influenced by macrophage activation. Second, suppression of malignant melanoma cell metastasis to lung were induced by macrophage and NK cell activation in spleen with red ginseng acidic polysaccharide (RGAP). Third, final metabolites of M1, M4 had exerted anticancer effect of ginseng. Conclusion: Unknown anticancer mechanisms of ginseng have been studied for many years up until now. Ginseng is comprised of multiple bio-chemical compounds that create complex pharmaceutical interactions. Therefore, for its proper usage and safe prescription, studies on different types of ginseng and patients' susceptibility to ginseng according to their constitution and stages of the disease should be further pursued. More efforts are needed to understand the anticancer mechanisms of ginseng as well.

• Korean Journal of Veterinary Research
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• v.35 no.2
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• pp.317-326
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• 1995

The purpose of this study was to establish a method for in vitro culture of C parvum isolated in Korea by determination of suitable cell model to complete development of this parasite. The result obtained were summerized as follows: 1. To determine the most suitable cell line, six types of cell line were examined by microscopy. All cell lines were infected with C parvum and showed the highest infection score in HmLu cells. 2. The staining methods including DMSO-modified acid-fast(A-F) stain, hematoxylin-eosin(H & E) stain and immunofluorescence antibody(IFA) stain were applied to examine the infection of C parvum in cell culture. These staining methods were possible to examine the infection of C parvum in cell culture. The most sensitive one was IFA staining technique. 3. Developmental stages of C parvum in HmLu cell were observed. After the initial 8 hour incubation period, some trophozoites were observed. The meronts and gametes were appeared at 24-48 hour post inoculation(PI), and oocysts were observed firstly at 48-72 hour PI. 4. In H & E stain, the parasite appeared as basophilic within parasitophorous vacuole membrane(PVM) and lying in cytoplasm at near the nucleus of the host cells. It was able to distinguish the type I, type II meronts and gametes. 5. In DMSO-modified acid-fast stain, specific stained parasites were appeared firstly after 48 hour PI. The parasites were showed with different degrees of staining bright red color within PVM. 6. The endogenous stages of parasites in HmLu cell recovered at 48, 96, 120 and 144 hour after inoculation were reacted with rabbit immunized serum in immunofluorescence antibody and avidin-biotin complex peroxidase staining technique.

• Journal of the Korean Society of Costume
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• v.59 no.4
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• pp.1-13
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• 2009

The purpose of this study is to examine the types of and figurative characteristics of in women's embroidered handicrafts of Korea. It also aims to revive Korean traditional beauty, to examine the applicability of Korean traditional design that has both modern beauty and practicality, and to develop Korean women's embroidered handicrafts. The types of women's embroidered of Korea include Jumoni (pouches), Bojagi (wrapping cloths), and other accessories made by sewing. Jumoni consisting of 55.51% of them was the most frequently used handicraft followed by Bojagi (8.66%), and other accessories (35.83%). In terms of the patterns used, animal pattern and plant pattern were used the most frequently. In addition, complex patterns using two or three patterns together(70.49) were more frequent than singular patterns (29.53%). Pictorial type of pattern arrangement (58.24%) was used the most frequently and dense type (39.76%) and sparse type (2.00%) were followed. In terms of the materials on which embroidery was used, Dahn (85.82%) was used the most frequently followed by wool (5.67%), cotton (4.26%), silk (3.55%), and Sa (0.70%). Various techniques of stitches were used such as outline stitch (40.56%), satin and running stitch (29.32%), French nut stitch (28.11%), long and short stitch (21.28%), double leaf stitch (20.88%), and so on. Among them, satin stitch (63.05%) was the most popular type of stitch techniques. Strings (23.56%), maedeup (Korean traditional knots, 8.62%), or tassels (9.77%) were also attached for the purpose of both practicality and of decoration. There were some cases that added cloissone or red-pepper shaped decorations.

• BMB Reports
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• v.49 no.5
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• pp.245-246
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• 2016

The level and activity of critical regulatory proteins in cells are tightly controlled by several tiers of post-translational modifications. HIF-1α is maintained at low levels under normoxia conditions by the collaboration between PHD proteins and the VHL-containing E3 ubiquitin ligase complex. We recently identified a new physiologically relevant mechanism that regulates HIF-1α stability in the nucleus in response to cellular oxygen levels. This mechanism is based on the collaboration between the SET7/9 methyltransferase and the LSD1 demethylase. SET7/9 adds a methyl group to HIF-1α, which triggers degradation of the protein by the ubiquitin-proteasome system, whereas LSD1 removes the methyl group, leading to stabilization of HIF-1α under hypoxia conditions. In cells from knock-in mice with a mutation preventing HIF-1α methylation (Hif1α^KA/KA), HIF-1α levels were increased in both normoxic and hypoxic conditions. Hif1α^KA/KA knock-in mice displayed increased hematological parameters, such as red blood cell count and hemoglobin concentration. They also displayed pathological phenotypes; retinal and tumor-associated angiogenesis as well as tumor growth were increased in Hif1α^KA/KA knock-in mice. Certain human cancer cells exhibit mutations that cause defects in HIF-1α methylation. In summary, this newly identified methylation-based regulation of HIF-1α stability constitutes another layer of regulation that is independent of previously identified mechanisms.

• Journal of Periodontal and Implant Science
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• v.41 no.4
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• pp.167-175
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• 2011

Purpose: Periodontal ligament (PDL) cell differentiation into osteoblasts is important in bone formation. Bone formation is a complex biological process and involves several tightly regulated gene expression patterns of bone-related proteins. The expression patterns of bone related proteins are regulated in a temporal manner both in vivo and in vitro. The aim of this study was to observe the gene expression profile in PDL cell proliferation, differentiation, and mineralization in vitro. Methods: PDL cells were grown until confluence, which were then designated as day 0, and nodule formation was induced by the addition of 50 ${\mu}g$/mL ascorbic acid, 10 mM ${\beta}$-glycerophosphate, and 100 nM dexamethasone to the medium. The dishes were stained with Alizarin Red S on days 1, 7, 14, and 21. Real-time polymerase chain reaction was performed for the detection of various genes on days 0, 1, 7, 14, and 21. Results: On day 0 with a confluent monolayer, in the active proliferative stage, c-myc gene expression was observed at its maximal level. On day 7 with a multilayer, alkaline phosphatase, bone morphogenetic protein (BMP)-2, and BMP-4 gene expression had increased and this was followed by maximal expression of osteocalcin on day 14 with the initiation of nodule mineralization. In relationship to apoptosis, c-fos gene expression peaked on day 21 and was characterized by the post-mineralization stage. Here, various genes were regulated in a temporal manner during PDL fibroblast proliferation, extracellular matrix maturation, and mineralization. The gene expression pattern was similar. Conclusions: We can speculate that the gene expression pattern occurs during PDL cell proliferation, differentiation, and mineralization. On the basis of these results, it might be possible to understand the various factors that influence PDL cell proliferation, extracellular matrix maturation, and mineralization with regard to gene expression patterns.

• Journal of The Korean Astronomical Society
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• v.37 no.4
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• pp.285-288
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• 2004

Near-infrared imaging photometry supplemented by optical spectroscopy and narrow-band imaging of the H II region Sh 2-128 and its environment are presented. This region contains a developed H II region and the neighboring compact H II region S 128N associated with a pair of water maser sources. Midway between these, the core of a CO cloud is located. The principal ionizing source of Sh 2-128 is an 07 star close to its center. A new spectroscopic distance of 9.4 kpc is derived, very similar to the kinematic distance to the nebula. This implies a galactocentric distance of 13.5 kpc and z = 550 pc. The region is optically thin with abundances close to those predicted by galactocentric gradients. The $JHK_s$ images show that S 128N contains several infrared point sources and nebular emission knots with large near-infrared excesses. One of the three red Ks knots coincides with the compact H II region. A few of the infrared-excess objects are close to known mid- and far-infrared emission peaks. Star counts in J and $K_s$ show the presence of a small cluster of B-type stars, mainly associated with S 128N. The $JHK_s$ photometric properties together with the characteristics of the other objects in the vicinity suggest that Sh 2-128 and S 128N constitute a single complex formed from the same molecular cloud, with ages ${\~}10^6$ and < $3 {\times} 10^5$ years respectively. No molecular hydrogen emission was detected at 2.12 ${\mu}m$. The origin of this remote star forming region is an open problem.

• Korean Journal of Food Science and Technology
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• v.28 no.3
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• pp.421-427
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• 1996

Ethyl carbamate is an animal carcinogen and a suspected human carcinogen found in fermented foods and beverages. For the determination of ethyl carbamate in typical Korean diet, an analytical method was established for the food as complex as Kimchi. Kimchi samples collected from various locations in the country were homogenized and extracted four times with ethyl acelate. Following concentration and reconstitution with water, the extract was loaded onto $C_{18}$ column. Fraction containing ethyl carbamate was eluted with methanol, while most of the red pigment of the sample was retained on the column. The eluent was further purified with alumina, followed by Florisil column. The final eluent was analyzed by gas chromatography mass spectrometry in the selected ion monitoring mode. None of the twenty Kimchi samples showed ethyl carbamate level higher than 4.6 ppb without correction for the recovery. The concentration of ethyl carbamate in Kimchi increased as pH decreased, suggesting fermentation dependent formation of ethyl carbamate.

• Journal of Periodontal and Implant Science
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• v.48 no.1
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• pp.47-59
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• 2018

Purpose: To evaluate the clinical and microbiological effects of the local use of egg yolk immunoglobulin against Porphyromonas gingivalis (anti-P.g. IgY) as an adjunct to scaling and root planing (SRP) in the treatment of moderate to severe chronic periodontitis. Methods: This was a randomized, placebo-controlled, double-blind trial involving 60 systematically healthy patients with moderate to severe chronic periodontitis. Subjects (n=20/group) were randomly assigned to receive SRP combined with subgingival irrigation of anti-P.g. IgY and anti-P.g. IgY mouthwash, subgingival irrigation of 0.2% chlorhexidine and 0.2% chlorhexidine mouthwash, or subgingival irrigation of placebo and placebo mouthwash for 4 weeks. Probing pocket depth, clinical attachment level, bleeding on probing, and the plaque index were evaluated at baseline and at 4 weeks. Subgingival plaque, gingival crevicular fluid, and saliva were simultaneously collected for microbiological analysis. Results: Our results showed that anti-P.g. IgY mouthwash was as effective as chlorhexidine at improving clinical parameters over a 4-week period. All the groups showed a significant reduction in levels of P.g. at 4 weeks. No significant difference was observed in the test group when compared to placebo regarding the reduction in the levels of P.g. Anti-P.g. IgY significantly suppressed the numbers of red complex bacteria (RCB) in subgingival plaque and saliva in comparison with placebo. No adverse effects were reported in any of the subjects. Conclusions: Within the limitations of the study, the present investigation showed that passive immunization with anti-P.g. IgY may prove to be effective in the treatment of chronic periodontitis due to its ability to improve clinical parameters and to reduce RCB. No significant differences were found between the anti-P.g. IgY and placebo groups in the reduction of P.g.

• Publications of The Korean Astronomical Society
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• v.30 no.2
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• pp.219-221
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• 2015

We present our recent observations of SDSS J102102.25+174439.9, a new eclipsing white dwarf - main sequence WDMS binary with an orbital period of 0.14 days. This system belongs to the post common-envelope binary group as shown by the spectrum from the Sloan Digital Sky Survey. We obtained our data using the ULTRASPEC instrument installed on the 2.4-m telescope at the Thai National Observatory (TNO). Our multi-band observations reveal an unusual and persistent drop in brightness after the primary eclipse. These dips, which appear to show variations in amplitude, also have a complex shape that changes within days. Dips in WDMS systems have been observed on only one other occasion, in the light curve of QS Vir prior to the eclipse of the white dwarf. The dips in SDSS J1021+1744 are unique because they are present at different wavelengths and they occur approximately at similar phases. Hosting a DA white dwarf and an M4 companion star, this system is known to be the only WDMS to show these kind of dips in its light curve. It is possible that these dips are caused by ejected materials from an active companion star, such as in QS Vir. The light curve in the g' filter exhibits deep and narrow features, implying that the material which passes in front of the white dwarf in SDSS J1021 must be dense and small in size. Furthermore, we try to constrain the stellar and orbital parameters of SDSS J1021+1744 using the Binary Maker 3 software. We use g' and r' data for our light curve analysis to have a better approximation for the red dwarf star.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.36 no.4
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• pp.205-211
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• 2018

Remote estimation of water quality via radiometric instruments provides a convenient means for monitoring environmental changes in water bodies in wide areas. Combined with platforms such as satellite, manned/unmanned vehicles, it reduces the measurement cost and time for acquiring water quality information on the interested target areas. To develop accurate retrieval algorithms, however, acquisition of in-situ measurements from various optical environment is critical. In this study, hyperspectral radiometric measurements, the coincident water quality variables, and its optical properties were obtained to analyze the optical environment of the study area. Field data collected around the Tongyeong area showed that the area has optically complex environment, with occasional outbreak of red tide in summer seasons. Effect of water constituents on the optical variables (remote sensing reflectance and absorption coefficients) were qualitatively analyzed.