• Proceedings of the Korean Society of Organic Agriculture Conference
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• 2008.12a
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• pp.133-138
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• 2008

1) 두과작물의 생초수량과 건물수량은 작물별로 차이가 컸다. 지상부는 Red clover가 생초수량 38.6 ton/ha, 건물수량 7.86 ton/ha로 가장 많았으며, 지하부 또한 Red clover가 생초수량 30 ton/ha, 건물수량 6.2 ton/ha로 가장 많았다. 2) CP 수량 및 TDN 수량은 Red clover가 각각 1 ton/ha, 4.6 ton/ha로 유의하게 높았으며 NDF와 ADF는 수확시기가 늦을수록 높은 경향을 나타내서 Galega와 Cicer milk vetch가 가장 높았다. 또한 상대사료가치(RFV)는 공시품종 대부분이 100 이상을 나타내어 사료가치가 우수하였으며 Yellow blossom sweet clover, Crimson clover, Hairy vetch 등은 151% 이상으로 1등급에 속하였다. 3) 가축사육능력은 $K_{cp}$(2.18 head/ha/yr) > $K_{TDN}$(1.25 head/ha/yr) >K(0.04 head/ha/yr) 순으로 높게 나타났으나 이는 상당히 낮은 수준이었으며 이는 두과작물의 적은 수량으로 인한 결과라 판단되어진다. 4) 두과작물과 화본과작물 혼파시 $K_{cp}$ $K_{TDN}$ 모두 두과작물 평균 2.18, 1.25보다 높았다. 또한 Rye-Galega 8:2 비율, Rye-Winter pea 7:3 비율에서 건물수량, $K_{cp}$ $K_{TDN}$이 화본과 단파시보다 증가하여 혼파효과가 인정되었다. 특히 건물수량과 $K_{TDN}$ 관계에서 혼파 비율에 따라 건물수량에 큰 차이를 보이지 않은 반면 $K_{TDN}$은 모든 비율에서 증가하여 사료가치가 상승하였다.

• Journal of Life Science
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• v.7 no.3
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• pp.192-197
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• 1997

Italian ryegrass, red clover, sorghum, and alfalfa were used for leaf protein preparation. Fresh leaves were pulped in the presence or absence of a reducing agent(sodium ascorbate or NaHSO$_{4}$) and green juice was heated and washed with acetone. The biological evaluation of leaf proteins was carried out by the growth method with male rats weighing about 45g. Italian ryegrass, red clover, and sorghum were brown when leaves were pulped in the absence of a reducing agent. On the other hand, alfalfa had neither o-diphenolics nor polyphenolase, and hence the alfalfa leaf protein did not brown during pulping ever in the absence of a reducing agent. The brown leaf protein from Italian ryegrass hd lower digestibility than the leaf protein protected from browning, although there were no difference in growth-promoting effect and protein efficiency ratio(PER)between the two leaf protein. The feeding of brown leaf protein from red clover resulted in the lowering of weight gain, digestibility, and PER, and all the measurement including diet intake were lowered by feeding the brown leaf protein from sorghum. In the case of alfalfa leaf protein, there were no difference in nutritional quality between the two leaf protein made with and without an attempt to prevent browning. The results mentioned above indicate that the occurrence of phenolics and polyphemolase in a crop in responsible for the browning of leaf protein and that the browning of leaf protein caused its nutritional impairment.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.39 no.4
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• pp.211-221
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• 2021

The Rabbit grass(Trifolium Repens, call it 'Clover') is a representative harmful plant of lawn, and it starts growing earlier than lawn, forming a water pipe on top of the lawn and hindering the photosynthesis and growth of the lawn. As a result, in competition between lawn and clover, clover territory spreads, but lawn is damaged and dried up. Damage to the affected lawn area will accelerate during the rainy season as well as during the plant's rear stage, spreading the area where soil is exposed. Therefore, the restoration of damaged lawn is causing psychological stress and a lot of economic burden. The purpose of this study is to distinguish clover which is a representative harmful plant on lawn, to identify the distribution of damaged areas due to the spread of clover, and to review of changes in vegetation before and after the eradication of clover. For this purpose, a time series analysis of three vegetation indices calculated based on images of convergence Drone with RGB(Red Green Blue) and BG-NIR(Near Infra Red)sensors was reviewed to identify the separation between lawn and clover for selective eradication, and the distribution of damaged lawn for recovery plan. In particular, examined timeseries changes in the ecology of clover before and after the weed-whacking by manual and brush cutter. And also, the method of distinguishing lawn from clover was explored during the mid-year period of growth of the two plants. This study shows that the time series analysis of the MGRVI(Modified Green-Red Vegetation Index), NDVI(Normalized Difference Vegetation Index), and MSAVI(Modified Soil Adjusted Vegetation Index) indices of drone-based RGB and BG-NIR images according to the growth characteristics between lawn and clover can confirm the availability of change trends after lawn damage and clover eradication.

• Journal of Life Science
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• v.15 no.2 s.69
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• pp.273-278
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• 2005

Trifolium pratense (red clover, Fabaceae) is a short-lived herbaceous species and the species is introduced from Europe or North America to Korea approximately 60 years ago. Allozyme variability was examined in populations representing this species. A high level of genetic variation was found in T. pratense populations. Ten of 19 loci $(52.6\%)$ showed detectable polymorphism. Genetic diversity was 0.220. The sexual reproduction, high fecundity, and colonization process are proposed as possible factors contributing to high genetic diversity. Genetic diversity (0.220) was lower than that (0.285) of North American red clover, T. pratense. Korean populations of red clover may be founded by a small sample of larger or moderate populations. An indirect estimate of the number of migrants per generation (Nm = 4.20) indicated that gene flow was extensive among Korean populations of this species.

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.4
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• pp.199-202
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• 1991

The esterase isozyme of several legume plants were separated and visualized by horizontal starch gel electrophoresis using enzyme-specific staining. Extracts used were prepared from fully expanded young leaf and stem of six legume species which were red clover(Trifolium Pretense L.), ladino clover(Trifolium repense L.), wild white clover(Trifolium repense L.), alfalfa(Medicage sativa L.), mimosoides(Cassia mimosoides var nomame Makino), and amoena(Vicia amoena Fisch). The number of band, Phenotype and staining intensity of esterase isozyme in leaf and stem varies depending on the plant species. However, there are little difference between leaf and stem esterase isozyme in same species except alfalfa. And in the leaf and stem of mimosoides and amoena showed not any esterase(Fig. 2). Among the examined plants, the highest staining intensity and the rapidest migrating esterase isozyme was Est 1.

• Journal of The Korean Society of Grassland and Forage Science
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• v.25 no.3
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• pp.151-158
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• 2005

This experiment was conducted to evaluate forage production and quality, and soil improvement of three legumes at Cheonan Yonam College. The three legumes used in the experiment were crimson clover (Trifolium incarnatum L.) red clover(Trifolium pratense L.) and chinese milk vetch(Astragalus sinicus L.). Flowering stage was observed on the 20th of April for chinese milk vetch and on the 30th of April for crimson clover, but the red clover did not produce flower until harvest time. The dry matter(DM) content of crimson clover was the highest among the three legumes. In terms of DM yield, crimson clover has the highest yield, the highest yield in crude protein(CP) and total digestible nutrients(TDN) was red clover. In terms of forage quality, the highest CP content was red clover, while the chinese milk vetch was the lowest for both neutral detergent fiber(NDF) and acid detergent fiberr(ADF). Red clove. and chinese milk vetch were highest fur TDN content, and the chinese milk vetch has the highest for relative feed value(RFV). Assessment of the chemical properties of soil after harvest showed that the nitrogen content of soil increased in all legumes. The organic matter(OM) content of soil in both crimson and red clover increased, while the chinese milk vetch decreased. The available $P_2O_5$ decreased in all three legumes, but the biggest decrease was in the chinese milk vetch. The exchangeable potassium in the soil for both crimson and red clover increased, but in chinese milk vetch it decreased. Based on the results of this study, the chinese milk vetch was superior in terms of forage quality, but crimson and red clover are excellent in forage yield and in maintaining soil quality in upland.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.5
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• pp.563-566
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• 2010

Using an open-circuit system, we compared the methane ($CH_4$) emission from dry cows fed first-cut Timothy silage ($1^{st}$ TY), second-cut Timothy silage ($2^{nd}$ TY), second-cut Italian ryegrass silage ($2^{nd}$ IR), third-cut Italian ryegrass silage ($3^{rd}$ IR), or second-cut red clover silage ($2^{nd}$ RC) as the sole feed. The methane emission ranged from 258.2 L $day^{-1}$ to 396.5 L $day^{-1}$. The methane emission from dry cows fed red clover silage was relatively lower than that from dry cows fed grass silage. However, the methane emission per unit digestible neutral detergent fiber (NDF) intake (dNDFI) did not differ significantly between the experimental silages. The methane emission was significantly correlated with the NDF intake and digestibility. Methane emission had a significant correlation with the quadratic function of dNDFI. The differences in the daily volume of methane emission from cows fed different forages can be explained by dNDFI.

• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.1
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• pp.7-12
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• 1994

This study was undertaken to evaluate the effect of legume reseeding on legume establishment, dry matter yield, quality and botanical composition of grasses in timothy(Ph1eum prarense L.) dominated pasture at alpine area. Establishment number and percentage of legume in timothy dominated pasture were increased with spring, red clover(Tr$olium pratense L.) and gramoxone $treatment Dry matter yield of pasture was increased with fall and grazing after overseeding, but, there waq no difference in legume species. Crude protein content(%) of grasses at the first cutting was increased with red clover and paraquat (gramoxone) treatment. Based on the results, it is suggested that spring in seeding time, red clover in legume species and oveneeding after gramoxone treatment are good for effective renovation of timothy dominated pasture at alpine area.

• Journal of Plant Biology
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• v.10 no.1_2
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• pp.36-40
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• 1967

1. The effect of the pH value on P32 absorption of leaves of three forest trees under the water culture is investigated. 2. The degree of the P32 absorption to the pH value is quite different in each forest tree. 3. The phosphorus contents are proved higher in the leaves of Quercus accutissima(the saw shaped oak) than in the leaves of Pinus densiflora(the red pine) and Lespedeza bicolar (bush clover). 4. Larger contents of phosphorus are found in acidity plots than in alkalinity. The leaves of treated plot of the pH 4 (red pine), of the pH 5.0 (bush clover), and of the pH 5.0-6.5 (saw shaped oak) have a higher nutrient capital as indicated by P32 compared with that of the other pH values. 5. It is also noticed that P32 absorption capacity is decreased with the higher pH values. 6. The content of P of leaves shows the lowest value in the plot of the pH 6.5 (pine), of the pH 7.5(bush clover), and of the pH 9.0(saw shaped oak). It is also noticed that the red pine is to do very well in acid cultivation and then follow bush clover and saw shaped oak in the order.

• Food Science and Preservation
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• v.16 no.2
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• pp.292-298
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• 2009

This study investigated the microbial safeties and germination rates of five domestic sprout species(alfalfa, broccoli, clover, red cabbage, and red radish) grown from disinfected seeds. The 48 h germination rates of all seeds were over 90%, regardless of treatment. Seed total plate count(TPC) and coliform levels were reduced significantly(p<0.05) by treatment with 20,000 ppm calcium hypochlorite solution at $25^{\circ}C$ for 15 min, following FDA recommendations. However, after germination, all sprouts regardless of treatment exhibited bacterial counts of $10^7-10^8CFU/g$. Listeria monocytogenes was detected at $10^3-10^4CFU/g$ on germinated non-disinfected clover seeds at days 1, 2, and 5. In conclusion, although sprout germination from disinfected seeds potentially permits the growth of sprouts with lower pathogen counts, there were no significant differences in TPC or coliform levels between sprouts grown from disinfected seeds and control sprouts. Further work is needed to improve the microbial safety of cultivated sprouts and to find optimal conditions for seed germination.