• 대한수의학회지
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• 제46권4호
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• pp.387-393
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• 2006

Progressing to the iron deficiency anemia was experimentally induced in 4 clinically healthy dogs by repeated phlebotomy to characterize hematologic features, serum iron values, and RBC indices. Abnormal RBC morphologies were also evaluated semiquantitatively on Wright's-stained blood films. Hematologic abnormalities in early stage of anemia included decreased both hematocrit and hemoglobin, and reticulocytosis, with no changes in mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC) were represented. In intermediate stage, decreased serum iron concentration with microcytosis and hypochromia were prominent. In late stage, red cell distribution width and Mentzer's index were out of reference ranges in the majority of dogs. In this study microcytic anemia was appeared at the hemoglobin range of 5.1-7.2 g/dl. On most sampling days, platelet counts and white blood cells were within the reference ranges, with some minor variations. Iron deficiency was not necessarily associated with microcytic anemia. Judging from the sequential changes of both MCV and MCHC, 3 patterns of anemia were sequentially observed: initially normocytic normochromic, intermediate normocytic hypochromic or normocytic normochormic, and finally microcytic hypochromic. The most frequent morphologic abnormalities were target cells. Occasional elliptocyte, acanthocyte, stomatocyte, kinzocyte, dacrocyte and schistocyte were also noted on the blood films.

• 한국동물위생학회지
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• 제40권2호
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• pp.155-159
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• 2017

Korean goral (Neamorhedus caudatus) is registered as a natural monument number 217 by South Korea Cultural Heritage Administration. It is also recognized as the endangered species I by Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). In this study, blood samples of Korean goral were collected to make reference intervals. Blood sampling was conducted on 19 numbers of Korean gorals (ten females, nine males), which were reared in Korean Goral Restoration Center. For total samples, the reference intervals of hematological parameters were: white blood cell $7.69{\sim}10.87K/{\mu}{\Lambda}$; hematocrit 36.73~46.18%; red blood cell $10.72{\sim}12.86K/{\mu}{\Lambda}$; hemoglobin 12.79~15.14 g/dL; mean corpuscular volume 33.15~36.75 fL; mean corpuscular hemoglobin 11.53~12.23 pg; mean corpuscular hemoglobin concentration 32.64-35.91 g/dL and red blood cell distribution width 39.2~43.46%. For total samples of each parameters, the following results were obtained for serum biochemistry: glucose 111.81~153.77 mg/dL; blood urea nitrogen 22.35~28.91 mg/dL; creatine 1.22~1.84 mg/dL; phosphate 4.57~6 mg/dL; calcium 8.7~9.1 mg/dL; total protein 6.53~6.92 g/dL; albumin 3.1~3.48 g/dL; globulin 3.26~3.62 g/dL; alanine aminotransferase 56.7~158.56 U/L; aspartate aminotransferase 230.35~473.06 U/L; alkaline phosphatase 178.06~332.47 U/L; gamma-glutamyl transpeptidase 131.6-~181.24 U/L; total bilirubin 1.47~2.12 mg/dL; cholesterol 46.48~71.52 mg/dL; amylase 16.3~26.03 U/L; sodium 150.43~153.88 mmol/L; potassium 3.98~4.6 mmol/L and chlorine 109.48~113.26 mmol/L. The ranges of values were similar campared to previous studies except in the case of RDW value, which showed higher range than the RDW value of a previous study. The reference intervals from this study will be useful data for treatment and management of gorals.

• 한국동물위생학회지
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• 제40권1호
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• pp.7-14
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• 2017

The present study was undertaken to clarify the changes in hematological values of Korean indigenous goats according to age. Blood samples were collected from 16 goats (5 females and 11 males) from birth up to the age of 6 weeks and the levels of various hematological values were analyzed. The body weight was increased from 2.54 kg at birth to 6.41 kg at 6 weeks age. The erythrocytes (RBC) counts and hemoglobin (Hb) values of goats were decreased at 2 weeks after birth, and increased gradually from 4 weeks after birth. The hematocrit (Ht) values were decreased until 4 weeks after birth, and increased at 6 weeks after birth. The mean cell volume (MCV) and mean cell hemoglobin (MCH) were decreased until 6 weeks after birth. The mean cell hemoglobin concentration (MCHC) was increased slightly from 2 weeks after birth. The red cell distribution width (RDW) was increased significantly at 2 weeks after birth, and decreased from 4 weeks after birth. The platelet (PLT) counts were increased significantly from 2 weeks after birth. The mean platelet volume (MPV) was decreased at 2 weeks after birth, and increased gradually from 4 weeks after birth. The total white blood cell (WBC) counts and the mean absolute numbers of neutrophil, lymphocyte, monocyte and eosinophil were increased from 2 weeks after birth. In conclusion, the data obtained from this study may be valuable as a standard for interpreting results of hematological analyses in Korean indigenous goats.

• 대한임상검사과학회지
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• 제43권1호
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• pp.12-18
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• 2011

The purpose of this study was to evaluate the significance of higher than normal mean corpuscular volume (MCV) and lower than normal mean corpuscular hemoglobin concentration (MCHC) in 20 patients. The hemoglobin (Hb), red blood cell indices (MCV, MCH, MCHC,), red cell distribution width (RDW), serum ferritin, serum iron (Fe) and total iron binding capacity (TIBC) were measured and the transferrin saturation were calculated. 18 (90.0%) cases were categorized as chronic diseases. Chronic renal failure, malignancy, and bleeding were all related to the cases. The mean values of hemoglobin was $9.5{\pm}2.14g/dL$; the MCV was $29.0{\pm}2.8fL$; the MCH was $30.9{\pm}1.0pg$; the MCHC was $31.2{\pm}0.5%$; the RDW was $17.0{\pm}3.6%$; serum Fe was $39{\pm}21{\mu}g/dL$; the TIBC was $219.7{\pm}108.8{\mu}g/dL$; transferrin saturation was $19.2{\pm}9.9%$ and ferritin was $445.5{\pm}499.6{\mu}g/L$ in the patients. The WHO criteria for hemoglobin of patients confirms anemia in 18 of the 20 (90.0%) cases. Anemia of chronic disease was shown in 11 (73.3%) cases; acute gastric ulcer with hemorrhage in 1 (6.7%) case; iron deficiency anemia in 1 (6.7%) case; 2 patients (13.3%) were of normal cases. There were changes in the baseline Hb level results of the 19 (95.0%) cases while no change was shown in 1 case. As a result, diseases associated with anemia and bleeding where the MCV is higher than the normal range and MCHC is lower than normal range are considered relevant findings.

• Asian-Australasian Journal of Animal Sciences
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• 제25권3호
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• pp.361-368
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• 2012

Effect of vitamin C (VC) on biochemical, hematological and cortisol profile of growing Holstein calves during summer was investigated. Eighteen calves between 14 and 16 weeks of age were divided equally into two groups. One group received a diet supplemented with VC (20 g/d) for 60 days, while other non-supplemented diet fed group served as a control (CON). The temperature humidity index (THI) was recorded and computed during the experiment. From days 0 to 60, the THI exceeded 70. Blood samples were collected from the jugular vein of each calf at days 0, 15, 30, 45 and 60. Serum albumin and total protein decreased (p<0.05) in CON and VC calves with age. Serum glutamic-oxaloacetic transaminase concentrations were not affected by treatments. Serum creatinine, albumin and glutamic-pyruvic transaminase concentrations were higher in calves in the VC group than the CON group. While red blood cells, hemoglobin and hematocrit were lower (p<0.05) in VC calves, mean corpuscular volume, mean corpuscular hemoglobin, red blood cell distribution width and mean platelet volume were higher (p<0.05) in these VC supplemented calves. Leukocyte parameters including white blood cells and full term for lymphocytes were not affected by the treatments. Also, serum cortisol was not affected by treatments. At day 15, 30 and 45, the total VC in plasma was higher (p<0.05) in calves fed with VC. In conclusion, serum cortisols were not affected by plasma VC concentration, while some blood parameters were positively influenced in calves fed with VC.

• Environmental Analysis Health and Toxicology
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• 제15권4호
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• pp.123-130
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• 2000

택트 스위치 제조공정 침지액의 주성분인 2-bromopropane독성에 대한 연구로 최단기의 폭로로 농도를 달리하여 3주간 반복 투여 시험을 시행하여, 흰쥐의 혈액 및 세정관의 변화를 관찰하기 위해 투여기간 동안의 체중의 변화, 고환, 간, 신장 등의 장기무게, 혈액화학과 혈액학적 분석 및 고환의 병리조직의 변화 등을 관찰하여 2-bromopropane의 급성투여 조건에서의 중독현상을 비교.분석하였다. 농도를 달리하여 투여에 따른 체중변동은 통계적으로 유의한(P<0.05)체중의 감소를 나타내었다. 1.000 mg/kg 투여군에서 백혈구수, 적혈구수, 혈구용적과 혈색소 농도에서 유의한 변화(p<0.05)를 보였다. 조직병리학적 소견으로 정 소에서는 세정관의 정조세포와 정모세포의 괴사를 볼 수 있었고, 기저막의 비후, 세정관의 Sertoli세포는 광범위하게 세포질성 공포현상을 보여주고 있다. 또한 간질조직에서는 Leydig 세포의 증식을 볼 수 있었다. 2-bromopropane의 손상부위는 조혈과 생식계가 표적으로 생각되며, 고농도 투여가 저농도 투여에 비해 독성이 심하며 독성물질의 양-반응 관계를 보여주고 있다.

• Journal of Yeungnam Medical Science
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• 제10권1호
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• pp.58-67
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• 1993

1986년 5월부터 1991년 6월까지 약 5년간 영남대학교 외과대학 부속병원에 내원하여 혈액학적 소견상 빈혈이 동반된 환자 210명과 정상인 200명 도합 410명을 대상으로 Coulter Counter S-plus II로 일반혈액검사를 실시하여 MCV와 RDW 및 여러 적혈구 지수를 얻어서 빈혈의 새로운 분류를 시도하였고 외국인에 비하여 빈혈의 병인 양상이 다른 한국인의 혈액질환의 감별진단에 도움이 되는 가를 알아 보고자 본 연구를 시행하였다. 거의 모든 경우에 MCV의 증감은 MCH와 MCHC의 증감을 동반하였으나 RDW와는 무관하였다. 따라서 저색소성빈혈 혹은 정색소성빈혈등의 용어보다는 이질성빈혈 혹은 동질성빈혈의 용어를 사용함이 빈혈의 형태학적 분류에 더욱 의미있는 것으로 사료되었다. 이질성소구성빈혈에는 철결핍성빈혈이 속하였고 이질성정구성빈혈에는 급성백혈병과 골수로성빈혈이 여기에 속하였으며 이질성대구성빈혈에는 거대적아구성빈혈과 용혈성빈혈이 속하였다. 동질성소구성빈혈에는 만성질환에 의한 빈혈이 여기에 속하였으며 동질성정구성빈혈에는 급성출혈, 만성백혈병 및 다발성 골수종등에 의한 빈혈이 여기에 속하고 동질성대구성빈혈에는 재생활량성빈혈이 여기에 속하였다. 진단적인 의의가 큰 혈색소병은 본 연구에는 관찰되지 않았지만 한국인에 가장 많은 빈혈의 원인인 철결핍성빈혈과 만성질환에 의한 빈혈의 감별진단에 RDW가 유용한 것으로 생각되었다.

• Journal of Yeungnam Medical Science
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• 제8권1호
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• pp.154-165
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• 1991

1) 건강한 성인 2823명을 대상으로 자동혈구 계산기인 Coulter Counter Model S plus II를 이용하여 본원의 참고치를 설정하였다. 산출된 참고치는 기기의 설명서에 제시되어 임상 의사들이 사용하고 있는 참고치와 차이가 있었다. 2) 국내의 보고들과 비교해 볼 때 본원의 참고치와 대체로 비등하였고 기기의 설명서에 제시된 참고치와는 차이가 있으므로 본원의 참고치률 설정하여 사용하여야 하겠다. 3) 결과는 다음과 같다. (1) 백혈구 수는 남자 $6.800{\pm}2.680(2SD)/{\mu}l$, 여자 $5.950{\pm}2.380(2SD)/{\mu}l$ 이었다. (2) 적혈구 수는 남자 $428{\pm}60(2SD){\times}10^4/{\mu}l$, 여자 $415{\pm}56(2SD){\times}10^4/{\mu}l$ 이었다. (3) 혈색소량은 남자 $15.4{\pm}1.8(2SD)g/dL$, 여자 $13.0{\pm}1.6(2SD)g/dL$ 이었다. (4) 적혈구 용적은 남자 $45.3{\pm}5.0(2SD)%$, 여자 $38.2{\pm}4.6(2SD)%$ 이었다. (5) 평균 적혈구 용적은 남자 $93.8{\pm}5.8(2SD)fL$, 여자 $92.2{\pm}7.4(2SD)fL$ 이었다. (6) 평균 적혈구 혈색소량은 남자 $31.8{\pm}2.2(2SD)pg$, 여자 $31.4;{\pm}2.8(2SD)pg$ 이었다. (7) 평균 적혈구 혈색소농도는 남자 $34.0{\pm}1.2(2SD)%$, 여자 $33.9{\pm}1.2(2SD)%$ 이었다. (8) 적혈구 분포 폭은 남자 $12.7{\pm}1.0(2SD)%$, 여자 $12.6{\pm}1.4(2SD)%$ 이었다. (9) 혈소판 수는 남자 $242.9{\pm}87.8(2SD){\times}10^4/{\mu}l$, 여자 $242.2{\pm}89.0(2SD){\times}10^4/{\mu}l$ 이었다. (10) 혈소판 용적은 남자 $0.201{\pm}0.076(2SD)%$, 여자 $0.204{\pm}0.076(2SD)%$ 이었다. (11) 평균 혈소판 용적은 남자 $8.20{\pm}1.70(2SD)fL$, 여자 $8.36{\pm}1.82(2SD)fL$ 이었다. (12) 혈소판 분포 폭은 남자 $16.1{\pm}0.8(2SD)%$, 여자 $16.0{\pm}0.8(2SD)%$ 이었다.

• Asian-Australasian Journal of Animal Sciences
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• 제24권12호
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• pp.1711-1717
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• 2011

The objective of this experiment was to evaluate the effect of dietary supplementation of glutathione (GSH) on health, solid feed consumption, nutrient intake, body weight gain (BWG), feed efficiency, blood metabolites and the occurrence of diarrhea in Holstein neonatal calves. The calves were fed plain milk as a control (CON) or milk with GSH supplementation. Sixteen calves were separated from their mothers immediately after birth, moved into individual cages and fed colostrum for the first three days. For GSH supplementation, three grams of GSH powder were mixed in 1.8 L of heat-treated milk and placed in a plastic bottle with a rubber nipple. The calves were fed GSH-supplemented milk only once out of four daily feedings. For the first 25 d, calves were fed 1.8 L of milk four times per day. Milk feeding frequency was reduced to three times per day from days 26 to 30, followed by twice a day from days 31 to 44, and once a day from days 45 to 49, after which they were weaned at day 50. Body weight gain (BWG), feed consumption, and growth performance were monitored until day 70. The dietary supplementation of GSH had no effect on daily feed intake and growth performance in growing calves. Hematological results revealed red blood cell distribution width (RDW) was lower, and mean corpuscular volume (MCV) was significantly higher in calves fed GSH. Serum lactate dehydrogenase (LDH) concentrations were lower in calves fed GSH. Rectal temperature at day 70 was higher in calves that did not receive GSH, while mean frequency of diarrhea and enteritis was less in calves fed GSH. It is concluded from the present study that BW gain, total dry matter intake (DMI), feed efficiency, and breathing rate did not differ between groups. However, there were some positive blood parameters and the mean frequency of diarrhea and enteritis was less in calves fed GSH compared to CON which did not receive GSH. With the results obtained, supplementation of GSH is highly recommended.

• Journal of Preventive Medicine and Public Health
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• 제25권2호
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• pp.162-171
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• 1992

The hematologic effect by low-concentration and repeated exposure to mixed organic solvent remains obscure. This study was performed to evaluate the hematologic effect by mixed organic solvent exposure, especially on the type of anemia by mean corpuscular volume(MCV) and red blood cell distribution width(RDW). The subject were 64 organic solvent workers(male 4, female 60) and 78 general workers(male 18, female 60) who showed anemic tendency in worker's health examination which carried out by Pusan Paik Hospital from January to December, 1988. The author gained some hematologic findings(hemoglobin, hematocrit, RBC, WBC, MCV, MCHC, platelet count, ESR, RDW) from auto-analysis method by coulter counter S plus IV, and compared the type of anemia by MCV and RDW. In the pilot study for estimating the prevalence of anemia according to the type of b, the prevalence rate was higher in organic solvent workers than in public officials as 10.9% and 4.1% respectively. There were statistical significance in the value of hemoglobin, hematocrit, MCV, MCHC, platelet count, ESR, RDW between the two study groups with more severe anemic tendency in organic solvent workers. The type of anemia in organic solvent workers was microcytic and anisocytic with the mean value of $68.28{\pm}8.3fL$ of MCV and $19.1{\pm}4.0%$ of RDW.