• Title/Summary/Keyword: Reciprocal

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Evaluation of Reciprocal Cross Design on Detection and Characterization of Mendelian QTL in $F_2$ Outbred Populations

  • Lee, Yun-Mi;Kim, Eun-Hee;Kim, Jong-Joo
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.11
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    • pp.1625-1630
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    • 2007
  • A simulation study was conducted to evaluate the effect of reciprocal cross on the detection and characterization of Mendelian QTL in $F_2$ QTL swine populations. Data were simulated under two different mating designs. In the one-way cross design, six $F_0$ grand sires of one breed and 30 $F_0$ grand dams of another breed generated 10 $F_1$ offspring per dam. Sixteen $F_1$ sires and 64 $F_1$ dams were randomly chosen to produce a total of 640 $F_2$ offspring. In the reciprocal design, three $F_0$ grand sires of A breed and 15 $F_0$ grand dams of B breed were mated to generate 10 $F_1$ offspring per dam. Eight $F_1$ sires and 32 $F_1$ dams were randomly chosen to produce 10 $F_2$ offspring per $F_1$ dam, for a total of 320 $F_2$ offspring. Another mating set comprised three $F_0$ grand sires of B breed and 15 $F_0$ grand dams of A breed to produce the same number of $F_1$ and $F_2$ offspring. A chromosome of 100 cM was simulated with large, medium or small QTL with fixed, similar, or different allele frequencies in parental breeds. Tests between Mendelian models allowed QTL to be characterized as fixed (LC QTL), or segregating at similar (HS QTL) or different (CB QTL) frequencies in parental breeds. When alternate breed alleles segregated in parental breeds, a greater proportion of QTL were classified as CB QTL and estimates of QTL effects for the CB QTL were more unbiased and precise in the reciprocal cross than in the one-way cross. This result suggests that reciprocal cross design allows better characterization of Mendelian QTL in terms of allele frequencies in parental breeds.

Molecular characterization of reciprocal crosses of Aerides vandarum and Vanda stangeana (Orchidaceae) at the protocorm stage

  • Kishor, Rajkumar;Devi, H.S.;Jeyaram, K.;Singh, M.R.K.
    • Plant Biotechnology Reports
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    • v.2 no.2
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    • pp.145-152
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    • 2008
  • Aerides vandarum and Vanda stangeana are two rare and endangered vandaceous orchids with immense floricultural traits. The intergeneric hybrids were synthesized by performing reciprocal crosses between them. In vitro germination response of the immature hybrid embryos was found to be best on half-strength Murashige and Skoog medium supplemented with 20% (v/v) coconut water/liquid endosperm from tender coconut. Determination of hybridity was made as early as the immature seeds or embryos germinated in vitro, using randomly amplified polymorphic DNA (RAPD) markers. Out of 15 arbitrarily chosen decamer RAPD primers, two were found to be useful in amplification of polymorphic bands specific to the parental species and their presence in the reciprocal crosses. However, a decisive profile that can identify the reciprocal crosses could not be provided by RAPD. Amplification of the trnL-F non-coding regions of chloroplast DNA of the parent species and hybrids aided easy identification of the reciprocal crosses from the fact that maternal inheritance of chloroplast DNA held true for these intergeneric hybrids. Subsequent restriction digestion of the polymerase chain reaction (PCR) amplified trnL-F non-coding regions of chloroplast DNA also consolidated the finding. Such PCR-based molecular markers could be used for early determination of hybridity and easy identification of the reciprocal crosses.

The Effects of Scapula and Pelvis Symmetrical Reciprocal Pattern of PNF Concept on Respiratory Function and Quality of Life in the Elderly (PNF의 어깨뼈 골반 대칭 상호 패턴이 노인의 호흡기능과 삶의 질에 미치는 효과)

  • Byoung-Hyoun Moon;Ji-Won Kim
    • Journal of The Korean Society of Integrative Medicine
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    • v.12 no.2
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    • pp.55-63
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    • 2024
  • Purpose : The purpose of this study is to determine the effects of scapula and pelvis symmetrical reciprocal pattern exercise of PNF concept on respiratory function and quality of life in elderly subjects. Methods : Nineteen elderly subjects with healthy were recruited. Subjects performed scapula and pelvis symmetrical reciprocal pattern exercise of PNF concept. Exercise was appied 30 minutes three times per week for four weeks. The respiratory function and quality of life (QOL) test three times (before, two weeks, and four weeks). Respiratory function includes forced vital capacity (FVC), forced expiratory volume at one second (FEV1), Peak Expiratory Flow (PEF) and chest cage expansion test (CCET). The QOL test was measured Korean WHOQOL-BRIEF. The analysis method was analyzed through the one-way ANOVA repeated methods, and the statistical significance is α=.05. It was analyzed through the post test Bonferroni test. Results : After the 4 week scapula and pelvis symmetrical reciprocal pattern exercise of PNF concept showed statistically significant differences in the respiratory function (FVC, FEV1, PEF, and CCET), and QOL (p<.05). As a result of the post-hoc test, FVC showed a significant increase in the mid test and post test compared to the pre test (p<.05), FEV1 showed a significant increase in the post test compared to the pre test (p<.05). PEF showed a significant increase in all pre-test, mid-test, and post-test sections (p<.05), CCET showed a significant increase in all pre-test, mid-test, and post-test sections (p<.05). QOL showed a significant increase in all pre-test, mid-test, and post-test sections (p<.05). Conclusion : In this study, the scapula and pelvis symmetrical reciprocal pattern exercise of PNF concept improved respiratory function and QOL. The findings suggest that this intervention could be beneficial in improving respiratory function and QOL in the elderly.

Effects of Reciprocal Grafting between Varieties on Growth and Yield in Soybeans (콩 품종간의 상호접목이 생육과 수량에 미치는 영향)

  • 정승근;손석용;허성수
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.37 no.4
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    • pp.339-346
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    • 1992
  • Self-and reciprocal-graftings were made between soybean varieties(cv.Hwangkeumkong, Paldalkong, Bokwangkong and Jangkyongkong) to assertain the relative role of shoot and root in soybean growth and yield determination. The influence of grafting per se on plant height and leaf number on main stem were negligible as evidenced by the insignificant differences between self-and reciprocal-graftings. The effect of grafting on flowering was different between different set of varieties grafted reciprocally. Self grafting delayed flowering 2 days and reciprocal grafting delayed another 2 days, in general. The effects of grafting on dry weights of different plant parts except pod were also insignificant. Reciprocal grafting increased pod dry weight in Hwangkeumkong and Paldalkong regardless whether they were used as scion or stock. Reciporcal grafting also influenced dry weights of Bokwangkong and Jangkyongkong significantly, but the response was different from those of Hwangkeumkong or Paldalkong. Jangkyongkong, when used as stock, decreased total dry weight of Bokwangkong significantly through the reduction of pod and stem dry weights. Stem diameter was increased by grafting, however, grafting decreased number of branches in all varieties. The average yields of self-and reciprocal-grafting were decreased by 11% and 24%, respectively, comparing to that of non-grafting, while average yield of reciprocal grafting was 14% lower than that of self grafting. Stem weight of grafted soybeans was decreased by 29-32% comparing to that of non-grafted soybeans, but the difference between self and reciprocal graftings was negligible. The genetic characteristics of scion was found to be more important than those of stock in determination of yield and stem weight. It was also indicated that varieties and characteristics are different in response to grafting.

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AN UPPER BOUND OF THE RECIPROCAL SUMS OF GENERALIZED SUBSET-SUM-DISTINCT SEQUENCE

  • Bae, Jaegug
    • Journal of the Chungcheong Mathematical Society
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    • v.21 no.2
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    • pp.223-230
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    • 2008
  • In this paper, we present an upper bound of the reciprocal sums of generalized subset-sum-distinct sequences with respect to the first terms of the sequences. And we show the suggested upper bound is best possible. This is a kind of generalization of [1] which contains similar result for classical subset-sum-distinct sequences.

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Characteristics Change of Spatial and Temporal Parameters of Gait in Spastic Hemiplegic Patients by Reciprocal Inhibition (상호억제 기법에 의한 경직성 편마비 환자 보행의 공간적, 시간적 특성 변화)

  • Kim Jong-Soon;Lee Hyun-Ok;Ahn So-Youn;Koo Bong-Oh;Bae Sung-Soo
    • The Journal of Korean Physical Therapy
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    • v.16 no.4
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    • pp.59-79
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    • 2004
  • The purpose of this study was to determined the effects of reciprocal inhibition on spatial-temporal gait parameters in spastic hemiplegic patients through GaitRite system. The subjects were consisted 45 patients who had spastic hemiplegia due to stroke. All subjects randomly assigned to 3 group : manual reciprocal inhibition program group(manual group), neuromuscular electrical stimulation group(NMES group) and control group. The manual group received voluntary isometric contraction of pre-tibia muscle. The NMES group received neuromuscular electrical stimulation on tibialis anterior. The control group was not recieved any therapeutic intervention. Before and after experiments, spatial-temporal gait parameters and functional ambulatory profile was measure in all patients. The data of 30 patients who complete experimental course were statistically analysed. The results of this study were as following : 1. The percentage of change of functional ambulatory profile were markedly increased in manual group but statistically non significant(p>.01). 2. The percentage of change of gait velocity and cadence were markedly increased in manual group but statistically non significant(p>.01). 3. Asymmetry ratio of gait elements were more improved in manual group but statistically non significant(p>.01). 4. There were no statistical difference between pre-test and post-test with functional ambulatory profile, gait velocity, cadence and asymmetry ratios in NMES group(p>.01). 5. There were no statistical difference between pre-test and post-test with unctional ambulatory profile, gait velocity, cadence and asymmetry ratios in control group(p>.01). In conclusion, the present results revealed that reciprocal inhibition which produced by voluntary isometric contraction of pre-tibia muscle can be improved spatial-temporal gait parameters including functional ambulatory profile in hemiplegic patients. Therefore, reciprocal inhibition is useful to improve functional activities in hemiplegic patient. Further study should be done to analyze the effects of intervention duration of reciprocal inhibition, appropriate muscle contraction, optimal time to apply the reciprocal inhibition in more long period.

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The Change of ${\alpha}$-motor neuron excitability in Spastic Stroke Patients by Pre-tibia Muscle Isometric Contraction (전경골근 등척성 수축에 의한 경직성 뇌졸중 환자의 비복근 ${\alpha}$-운동 신경원 흥분 변화)

  • Kim, Jong-Soon;Lee, Hyun-Ok;Ahn, So-Youn
    • The Journal of Korean Academy of Orthopedic Manual Physical Therapy
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    • v.11 no.1
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    • pp.11-28
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    • 2005
  • Spasticity has been defined as "a motor disorder characterized by a velocity-dependent increased in tonic stretch reflexes with exaggerated tendon jerks, resulting from hyperexcitability of the stretch reflex, as one components of the upper motorneuron syndrome". Spasticity is one of the common symptoms of stroke patients and frequently interferes with the motor functions such as gait, posture and activities of daily living. Therefore, its management is becoming a major issue in physical therapy. The purpose of this study was to determined the effects of reciprocal inhibition by isometric contraction of pre-tibia muscle on spasticity in hemiplegic patients through Hoffmann reflex. The subjects were consisted 45 patients who had hemiplegia due to stroke. All subjects randomly assigned to 3 group: manual reciprocal inhibition program group(manual group), neuromuscular electrical stimulation group(NMES group) and control group. The manual group received voluntary isometric contraction of pre-tibia muscle. The NMES group received neuromuscular electrical stimulation on tibialis anterior. The control group was not received any therapeutic intervention. Before and after experiments, Hoffmann reflex, M-wave and Modified Ashworth scale was measure in all patients. The data of 30 patients who complete experimental course were statistically analysed. Modified Ashworth scale were significantly decreased after experiment in manual group(p<.01). The Hmax/Mmax ratios were significantly decreased after experiment in manual group(p<.o1). There were no statistical difference between pre-test and post-test with modified Ashworth scale in NMES group(p>.01). There were no statistical difference between pre-test and post-test with Hmax/Mmax ratios in NMES group(p>.01). There were no statistical difference between pre-test and post-test with modified Ashworth scale in control group(p>.01). There were no statistical difference between pre-test and post-test with Hmax/Mmax ratios in control group(p>.01). The present results revealed that reciprocal inhibition which produced by voluntary isometric contraction of pre-tibia muscle can be reduce spasticity of gastrocnemius. Therefore, reciprocal inhibition is useful to improve functional activities in hemiplegic patient. Further study should be done to analyse the effects of intervention duration of reciprocal inhibition, appropriate muscle contraction, optimal time to apply the reciprocal inhibition in more long period.

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The Reciprocal Effects of Deviant Self-Concept and Delinquent Behaviors Revisited: A Latent State-Trait Autoregressive Modeling Approach (청소년 비행과 일탈적 자아개념의 상호적 인과관계: 잠재 상태-특성 자기회귀 모델을 통한 재검증)

  • Eunju Lee;Ick-Joong Chung
    • Korean Journal of Culture and Social Issue
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    • v.16 no.4
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    • pp.447-468
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    • 2010
  • The purpose of this study was to attain a clearer understanding of the reciprocal effects of deviant self-concept and delinquent behaviors by applying a latent state-trait autoregressive modeling approach. Although traditional autoregressive cross-lagged (ARCL) modeling has been widely applied to test the longitudinal reciprocal relationship between the two constructs, it could produce misspecified findings if there were trait-like processes involved in this relationship. The latent state-trait autoregressive(LST-AR) modeling was applied to control trait effects of deviant self-concept and to examine the reciprocal causal relations between the two constructs. Data were taken from a sample of 3,449 eighth graders who were followed annually for 5 years from the Korea Youth Panel Study. The combining LST-AR model with ARCL model substantiated the reciprocal effects of deviant self-concept and delinquent behaviors, even after the stable trait component of deviant self-concept was taken into account. The present findings shed lights on the reciprocal effects of behaviors (i.e., delinquency) and self concepts (i.e., deviant self-concept). Not only did behaviors change corresponding self-concept, but the ways adolescents perceived themselves influenced their behaviors.

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Stiffness Analysis of a Low-DOF Parallel Manipulator using the Theory of Reciprocal Screws (역나선 이론을 이용한 저자유도 평행구조 기구의 강성해석)

  • Kim, Han-Sung
    • Proceedings of the KSME Conference
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    • 2004.11a
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    • pp.573-578
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    • 2004
  • This paper presents a methodology for the stiffness analysis of a low-DOF parallel manipulator. A low-DOF parallel manipulator is a spatial parallel manipulator which has less than six degrees of freedom. The reciprocal screws of actuations and constraints in each leg can be determined by making use of the theory of reciprocal screws, which provide information about reaction forces due to actuations and constraints. When pure force is applied to a leg, the leg stiffness is modeled as a linear spring along the line. For pure couple, it is modeled as a rotational spring about the axis. It is shown that the stiffness model of an F-DOF parallel manipulator consists of F springs related to actuations and 6-F springs related to constraints connected from the moving platform to the base in parallel. The $6{\times}6$ Cartesian stiffness matrix is obtained, which is the sum of the Cartesian stiffness matrices of actuations and constraints. Finally, a 3-UPU parallel manipulator is used as an example to demonstrate the methodology.

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