• International conference on construction engineering and project management
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• 2009.05a
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• pp.242-249
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• 2009

High-rise construction sites, especially those situated in spatially constrained urban areas, have difficulties in timely delivery of materials. Modern techniques such as Just-in-time delivery, and use of information technology such as Project Management Information System (PMIS), are targeted to improve the efficiency of the logistics. Such IT-driven management techniques can be further benefited from state-of-the-art devices such as Radio Frequency Identification (RFID) tags and Ubiquitous Sensor Networks (USN), which has resulted in notable achievements in automated logistics management at the construction sites. Based on those achievements, this research develops USN hardware toolkits for construction lifts, which aims to be automated the vertical material delivery by sensing the material information and routing it automatically to the right place. The gathered information from the sensors can also be used for monitoring the overall status. The developed system will be tested in the actual high-rise construction sites to assess the system's feasibility. The proposed system is being implemented using Zigbee communication modules and RFID sensor networks which will communicate with the intelligent palette system (previously developed by the authors). To support the system, a lift-mountable intelligent toolkit is under development. Its feasibility test will be conducted by applying the implemented system to a test bed and then analyzing efficiency of the system and the toolkit. The collected test data will be provided as a basis of autonomous vertical transport equipment development. From this research, efficient management of the material lift is expected with increased accuracy, as well as better management of overall construction schedule benefited from the system. Further research will be expected to develop a smart construction lift, which will eliminate the need for human supervision, thus enabling a real 'autonomous' operation of the system.

• Journal of Sensor Science and Technology
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• v.32 no.3
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• pp.131-139
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• 2023

Ascorbic acid plays a crucial role in the regulation of neurotransmitters and enzymes in the central nervous system. Maintaining an optimal level of ascorbic acid, which is between 0.6-2 mg/dL, is vital for preventing oxidative stress and associated health conditions, such as cancer, diabetes, and liver disease. Therefore, the detection of ascorbic acid is of the utmost importance. Electrochemical sensing has gained significant attention among the various detection methods, owing to its simplicity, speed, affordability, high selectivity, and real-time analysis capabilities. However, conventional electrodes have poor signal response, which has led to the development of modified electrodes with better signal response and selectivity. Carbon nanotubes (CNTs) and their composites have emerged as promising materials for the electrochemical detection of ascorbic acid. CNTs possess unique mechanical, electrical, and chemical properties that depend on their structure, and their large surface area and excellent electron transport properties make them ideal candidates for electrochemical sensing. Recently, various CNT composites with different materials and nanoparticles have been studied to enhance the electrochemical detection of ascorbic acid. Therefore, this review aims to highlight the significance of CNTs and their composites for improving the sensitivity and selectivity of ascorbic acid detection. Specifically, it focuses on the use of CNTs and their composites in electrochemical sensing to revolutionize the detection of ascorbic acid and contribute to the prevention of oxidative stress-related health conditions. The potential benefits of this technology make it a promising area for future research and development.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.28 no.5D
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• pp.587-595
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• 2008

Many studies on bus scheduling optimization have been done from the 1960s to recent years for establishing rational bus schedule plan that can improve convenience of bus passengers and minimize unnecessary runs. After 2000, as part of the Intelligent Transport Systems (ITS), the importance of the schedule management and providing schedule information through bus schedule optimization has become a big issue, and much research is being done to develop optimization models that will increase bus passenger convenience and, on the side of bus management, minimize unnecessary bus operation. The purpose of this study is to calculate the optimal bus frequency and create a timetable for each bus stop by applying DTR or DTRC model that use data for each bus stop and route segment. Model verification process was implemented using data collected from bus management system (BMS) and integrated transit-fare card system for bus route of Seoul's No. 472 line. In order to evaluate the reliability and uncertainty of optimal solution, sensitivity analysis was implemented for the various parameters and assumptions used in the bus scheduling model.

• Fire Science and Engineering
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• v.32 no.4
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• pp.75-85
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• 2018

The Grenpell tower fire in England in June of 2016 is a representative example of damage caused by a vertical fire spreading through external insulation. Organic insulation materials, which are widely used in external insulation, have the disadvantage that they have good insulation performance but are vulnerable to fire. Aluminum composite panels are used as exterior wall finishing materials, and plastics used in aluminum are regarded as the cause of vertical fire spread. Due to the steel frame used to secure the aluminum composite panel to the outer wall, a cavity is formed between the outer wall and outer wall finish. When a fire occurs on the outer wall, the flammable outer wall as well as the flame generated from the heat-insulating material spreads vertically through the cavity, resulting in damage to people and property. In Korea, material unit performance tests are carried out by the Ministry of Land, Infrastructure and Transport notice 2015 - 744. However, in the UK, the BS 8414 test is used to measure the vertical fire spreading time on the outer wall in real scale fire tests. In this study, the risk of external wall fire was evaluated in an actual fire by conducting a real scale wall fire test (BS 8414), which was carried out in Europe, using aluminum composite panels of semi-noncombustible materials suitable for current domestic standards. The purpose of this study was to confirm the limitations of material unit evaluation of finishing materials and to confirm the necessity of introducing a system to prevent the spread of outer wall fire through an actual scale fire test.

• Proceedings of the Korean Institute of Navigation and Port Research Conference
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• v.1
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• pp.27-31
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• 2006

GNSS Services and Applications are today in permanent evolution in all the market sectors. This evolution comprises: ${\bullet}$ New constellations and systems, being GALILEO probably the most relevant example, but not the only one, as other regions of the world also dwell into developing their own elements (e.g. the Chinese Beidou system). ${\bullet}$ Modernisation of existing systems, as is the case of GPS and GLONASS ${\bullet}$ New Augmentation services, WAAS, EGNOS, MSAS, GRAS, GAGAN, and many initiatives from other regions of the world ${\bullet}$ Safety of Life services based on the provision of integrity and reliability of the navigation solutions through SBAS and GBAS systems, for aeronautical or maritime applications ${\bullet}$ New Professional applications, based on the unprecedented accuracies and integrity of the positioning and timing solutions of the new navigation systems with examples in science (geodesy, geophysics), Civil engineering (surveying, construction works), Transportation (fleet management, road tolling) and many others. ${\bullet}$ New Mass-market applications based on cheap and simple GNSS receivers providing accurate (meterlevel) solutions for daily personal navigation and information needs. Being on top of this evolving market requires an active participation on the key elements that drive the GNSS development. Early access to the new GNSS signals and services and appropriate testing facilities are critical to be able to reach a good market position in time before the next evolution, and this is usually accessible only to the large system developers as the US, Europe or Japan. Jumping into this league of GNSS developers requires a large investment and a significant development of technology, which may not be at range for all regions of the world. Bearing in mind this situation, MAGIC appears as a concept initiated by a small region within Europe with the purpose of fostering and supporting the development of advanced applications for the new services that can be enabled by the advent of SBAS systems and GALILEO. MAGIC is a low cost platform based on the application of technology developed within the EGNOS project (the SBAS system in Europe), which encompasses the capacity of providing real time EGNOS and, in the near future, GALILEO-like integrity services. MAGIC is designed to be a testing platform for safety of life and liability critical applications, as well as a provider of operational services for the transport or professional sectors in its region of application. This paper will present in detail the MAGIC concept, the status of development of the system within the Madrid region in Spain, the results of the first on-field demonstrations and the immediate plans for deployment and expansion into a complete SBAS+GALILEO regional augmentation system.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.88-89
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• 2013

A variety of influenza A viruses from animal hosts are continuously prevalent throughout the world which cause human epidemics resulting millions of human infections and enormous industrial and economic damages. Thus, early diagnosis of such pathogen is of paramount importance for biomedical examination and public healthcare screening. To approach this issue, here we propose a fully integrated Rotary genetic analysis system, called Rotary Genetic Analyzer, for on-site detection of influenza A viruses with high speed. The Rotary Genetic Analyzer is made up of four parts including a disposable microchip, a servo motor for precise and high rate spinning of the chip, thermal blocks for temperature control, and a miniaturized optical fluorescence detector as shown Fig. 1. A thermal block made from duralumin is integrated with a film heater at the bottom and a resistance temperature detector (RTD) in the middle. For the efficient performance of RT-PCR, three thermal blocks are placed on the Rotary stage and the temperature of each block is corresponded to the thermal cycling, namely $95^{\circ}C$ (denature), $58^{\circ}C$ (annealing), and $72^{\circ}C$ (extension). Rotary RT-PCR was performed to amplify the target gene which was monitored by an optical fluorescent detector above the extension block. A disposable microdevice (10 cm diameter) consists of a solid-phase extraction based sample pretreatment unit, bead chamber, and 4 ${\mu}L$ of the PCR chamber as shown Fig. 2. The microchip is fabricated using a patterned polycarbonate (PC) sheet with 1 mm thickness and a PC film with 130 ${\mu}m$ thickness, which layers are thermally bonded at $138^{\circ}C$ using acetone vapour. Silicatreated microglass beads with 150~212 ${\mu}L$ diameter are introduced into the sample pretreatment chambers and held in place by weir structure for construction of solid-phase extraction system. Fig. 3 shows strobed images of sequential loading of three samples. Three samples were loaded into the reservoir simultaneously (Fig. 3A), then the influenza A H3N2 viral RNA sample was loaded at 5000 RPM for 10 sec (Fig. 3B). Washing buffer was followed at 5000 RPM for 5 min (Fig. 3C), and angular frequency was decreased to 100 RPM for siphon priming of PCR cocktail to the channel as shown in Figure 3D. Finally the PCR cocktail was loaded to the bead chamber at 2000 RPM for 10 sec, and then RPM was increased up to 5000 RPM for 1 min to obtain the as much as PCR cocktail containing the RNA template (Fig. 3E). In this system, the wastes from RNA samples and washing buffer were transported to the waste chamber, which is fully filled to the chamber with precise optimization. Then, the PCR cocktail was able to transport to the PCR chamber. Fig. 3F shows the final image of the sample pretreatment. PCR cocktail containing RNA template is successfully isolated from waste. To detect the influenza A H3N2 virus, the purified RNA with PCR cocktail in the PCR chamber was amplified by using performed the RNA capture on the proposed microdevice. The fluorescence images were described in Figure 4A at the 0, 40 cycles. The fluorescence signal (40 cycle) was drastically increased confirming the influenza A H3N2 virus. The real-time profiles were successfully obtained using the optical fluorescence detector as shown in Figure 4B. The Rotary PCR and off-chip PCR were compared with same amount of influenza A H3N2 virus. The Ct value of Rotary PCR was smaller than the off-chip PCR without contamination. The whole process of the sample pretreatment and RT-PCR could be accomplished in 30 min on the fully integrated Rotary Genetic Analyzer system. We have demonstrated a fully integrated and portable Rotary Genetic Analyzer for detection of the gene expression of influenza A virus, which has 'Sample-in-answer-out' capability including sample pretreatment, rotary amplification, and optical detection. Target gene amplification was real-time monitored using the integrated Rotary Genetic Analyzer system.

• BMB Reports
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• v.40 no.5
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• pp.757-764
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• 2007

Marbling of cattle meat is dependent on the coordinated expression of multiple genes. Cattle dramatically increase their intramuscular fat content in the longissimus dorsi muscle between 12 and 27 months of age. We used the annealing control primer (ACP)-differential display RT-PCR method to identify differentially expressed genes (DEGs) that may participate in the development of intramuscular fat between early (12 months old) and late fattening stages (27 months old). Using 20 arbitrary ACP primers, we identified and sequenced 14 DEGs. BLAST searches revealed that expression of the MDH, PI4-K, ferritin, ICER, NID-2, WDNMI, telethonin, filamin, and desmin (DES) genes increased while that of GAPD, COP VII, ACTA1, CamK II, and nebulin decreased during the late fattening stage. The results of functional categorization using the Gene Ontology database for 14 known genes indicated that MDH, GAPD, and COP VII are involved in metabolic pathways such as glycolysis and the TCA cycle, whereas telethonin, filamin, nebulin, desmin, and ACTA1 contribute to the muscle contractile apparatus, and PI4-K, CamK II, and ICER have roles in signal transduction pathways regulated by growth factor or hormones. The final three genes, NID-2, WDNMI, and ferritin, are involved in iron transport and extracellular protein inhibition. The expression patterns were confirmed for seven genes (MDH, PI4-K, ferritin, ICER, nebulin, WDNMI, and telethonin) using real-time PCR. We found that the novel transcription repressor ICER gene was highly expressed in the late fattening stage and during bovine preadipocyte differentiation. This information may be helpful in selecting candidate genes that participate in intramuscular fat development in cattle.

• International Journal of Highway Engineering
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• v.10 no.2
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• pp.159-166
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• 2008

Video image processing systems (VIPS) offer numerous benefits to transportation models and applications, due to their ability to monitor traffic in real time. VIPS based on a wide-area detection algorithm provide traffic parameters such as flow and velocity as well as occupancy and density. However, most current commercial VIPS utilize a tripwire detection algorithm that examines image intensity changes in the detection regions to indicate vehicle presence and passage, i.e., they do not identify individual vehicles as unique targets. If VIPS are developed to track individual vehicles and thus trace vehicle trajectories, many existing transportation models will benefit from more detailed information of individual vehicles. Furthermore, additional information obtained from the vehicle trajectories will improve incident detection by identifying lane change maneuvers and acceleration/deceleration patterns. However, unlike human vision, VIPS cameras have difficulty in recognizing vehicle movements over a detection zone longer than 100 meters. Over such a distance, the camera operators need to zoom in to recognize objects. As a result, vehicle tracking with a single camera is limited to detection zones under 100m. This paper develops a methodology capable of monitoring individual vehicle trajectories based on image processing. To improve traffic flow surveillance, a long distance tracking algorithm for use over 200m is developed with multi-closed circuit television (CCTV) cameras. The algorithm is capable of recognizing individual vehicle maneuvers and increasing the effectiveness of incident detection.

• Environmental and Resource Economics Review
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• v.21 no.4
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• pp.999-1024
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• 2012

The Purpose of this study was to assess the willingness to pay(WTP) for the new road construction plan(slip road of urban highway which was advertising extensively to reduce the time to the core of metropolitan area) on housing prices using contingent valuation method(CVM). And this study was an empirical study which was based on the consciousness of residents whose apartment was within the Suwon Si's new plan of 47th road construction zone. In this study, it was revealed that the resident's WTP for the plan of new road construction which was built on the neighborhood of apartment was within the 10 percent of housing prices that was similar to the standard declared price of MLTM(Ministry of Land, Transport and Maritime Affairs). And also revealed that the difference of sale prices of real estate company was 24 percent. The statistical assessment results show that both the resident's characteristic variables such as gender, incomes, size of apartment and the accessability variables such as distance to parks, schools, department stores, living facilities were positive effects on the prices of apartments significantly. Finally, the house has depreciated than that of MLTM and WTO since torpedoed the road construction plan. These results imply that, we have to take capital appreciation of the property into account due to road construction since the accessibility was very significantly positive internal effects on the apartment housing prices.

• The Korean Journal of Physiology and Pharmacology
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• v.20 no.2
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• pp.201-211
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• 2016

Although the antioxidant and cardioprotective effects of NecroX-5 on various in vitro and in vivo models have been demonstrated, the action of this compound on the mitochondrial oxidative phosphorylation system remains unclear. Here we verify the role of NecroX-5 in protecting mitochondrial oxidative phosphorylation capacity during hypoxia-reoxygenation (HR). Necrox-5 treatment ($10{\mu}M$) and non-treatment were employed on isolated rat hearts during hypoxia/reoxygenation treatment using an ex vivo Langendorff system. Proteomic analysis was performed using liquid chromatography-mass spectrometry (LC-MS) and non-labeling peptide count protein quantification. Real-time PCR, western blot, citrate synthases and mitochondrial complex activity assays were then performed to assess heart function. Treatment with NecroX-5 during hypoxia significantly preserved electron transport chain proteins involved in oxidative phosphorylation and metabolic functions. NecroX-5 also improved mitochondrial complex I, II, and V function. Additionally, markedly higher peroxisome proliferator-activated receptor-gamma coactivator-$1{\alpha}$ ($PGC1{\alpha}$) expression levels were observed in NecroX-5-treated rat hearts. These novel results provide convincing evidence for the role of NecroX-5 in protecting mitochondrial oxidative phosphorylation capacity and in preserving $PGC1{\alpha}$ during cardiac HR injuries.