• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.13 no.3
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• pp.181-186
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• 2015

It is necessary to develop an effective waste salt treatment technology for the minimization of radioactive waste generation from the pyroprocessing of spent nuclear fuel. For this reason, the separation characteristics of NdCl₃ from LiCl-KCl eutectic salt in a reactive distillation process using Li₂CO₃ or K₂CO₃ were observed. NdCl₃ was converted into oxychloride (NdOCl) or oxide (Nd₂O₃) in the reaction model between NdCl₃ and the carbonates using HSC-Chemistry, and this result was confirmed in the reactive distillation test of the LiCl-KCl-NdCl₃ system using the carbonates. Based on these results, the reactive distillation process conditions were determined to separate NdCl₃ into an oxide form (Nd₂O₃) which can be easily fabricated into a final waste form.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.3
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• pp.343-351
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• 2016

Although the chemical, physical, and nutritional properties of bovine milk have been extensively studied, only a few studies have attempted to characterize milk-synthesizing genes using RNA-seq data. RNA-seq data was collected from 21 Holstein samples, along with group information about milk production ability; milk yield; and protein, fat, and solid contents. Meta-analysis was employed in order to generally characterize genes related to milk production. In addition, we attempted to investigate the relationship between milk related traits, parity, and lactation period. We observed that milk fat is highly correlated with lactation period; this result indicates that this effect should be considered in the model in order to accurately detect milk production related genes. By employing our developed model, 271 genes were significantly (false discovery rate [FDR] adjusted p-value<0.1) detected as milk production related differentially expressed genes. Of these genes, five (albumin, nitric oxide synthase 3, RNA-binding region (RNP1, RRM) containing 3, secreted and transmembrane 1, and serine palmitoyltransferase, small subunit B) were technically validated using quantitative real-time polymerase chain reaction (qRT-PCR) in order to check the accuracy of RNA-seq analysis. Finally, 83 gene ontology biological processes including several blood vessel and mammary gland development related terms, were significantly detected using DAVID gene-set enrichment analysis. From these results, we observed that detected milk production related genes are highly enriched in the circulation system process and mammary gland related biological functions. In addition, we observed that detected genes including caveolin 1, mammary serum amyloid A3.2, lingual antimicrobial peptide, cathelicidin 4 (CATHL4), cathelicidin 6 (CATHL6) have been reported in other species as milk production related gene. For this reason, we concluded that our detected 271 genes would be strong candidates for determining milk production.

• Korean Journal of Applied Biomechanics
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• v.25 no.2
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• pp.175-182
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• 2015

Objective : The purpose of this study was to investigate difference in fascicle behavior of the medial gastrocnemius during the locomotion with varying intensities, such as gait and one-legged and two-legged vertical jumping. Methods : Six subjects (3 males and 3 females; age: $27.2{\pm}1.6yrs.$, body mass: $62.8{\pm}9.8kg$, height: $169.6{\pm}8.5cm$) performed normal gait (G) at preferred speed and maximum vertical jumping with one (OJ) and two (TJ) legs. While subjects were performing the given tasks, the hip, knee and ankle joint motion and ground reaction force was monitored using a 8-infrared camera motion analysis system with two forceplates. Simultaneously, electromyography of the triceps surae muscles, and the fascicle length of the medial gastrocnemius were recorded using a real-time ultrasound imaging machine. Results : Comparing to gait, the kinematic and kinetic parameters of TJ and OJ were found to be significantly different. Along with those parameters, change in the medial gastrocnemius (MG) muscle-tendon complex (MTC) length ($50.57{\pm}6.20mm$ for TJ and $44.14{\pm}5.39mm$ for OJ) and changes in the fascicle length of the MG ($18.97{\pm}3.58mm$ for TJ and $20.31{\pm}4.59mm$ for OJ) were observed. Although the total excursion of the MTC and the MG fascicle length during the two types of jump were not significantly different, however the pattern of length changes were found to be different. For TJ, the fascicle length maintained isometric longer during the propulsive phase than OJ. Conclusion : One-legged and two-legged vertical jumping use different muscle-tendon interaction strategies.

• Journal of the Korea Concrete Institute
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• v.18 no.1 s.91
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• pp.125-132
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• 2006

Recently, surface finishing and protection materials were developed to restore performance of the deteriorated concrete and inhibiting corrosion of the reinforcing-bar. For this purpose, surface protection agent as well as coatings are used. Coatings have the advantage of low Permeability of $CO_2,\;SO_2$ and water. However, for coatings such as epoxy, urethane and acryl, long-term adhesive strength is reduced and the formed membrane of those is blistered by various causes. Also when organic coatings are applied to the wet surface of concrete, those have a problem with adhesion. On the other hand, surface protection agent penetrates into pore structure in concrete through capillary and cm make a dense micro structure in concrete as a result of filling effect. Furthermore, the chemical reaction between silicate from surface protection agent and cement hydrates can also make a additional hydration product which is ideally compatible with concrete body. The aim of this study is to examine the effect of penetrative surface protection agent(SPA) by evaluating several concrete durability characteristics. The results show that the concrete penetrated surface protection agent exhibited higher durability characteristics for instance, carbonation velocity coefficient, resistance to chemical attack and chloride ion penetration than the plain concrete. These results due to formation of a discontinuous macro-pore system which inhibits deterioration factors of concrete by changed the pore structure(porosity and pore size distributions) of the concrete penetrated surface protection agent.

• Journal of the Korea Institute of Building Construction
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• v.9 no.5
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• pp.63-70
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• 2009

This study is part of an ongoing research project on the development of a sound-absorbing lightweight foamed concrete manufactured by a hydro-thermal reaction between silica and calcium. As the silica source, pulverized bottom ash was used, and as several cementitious powders of ordinary portland cement, alumina cement and calcium hydroxide were used. Manufacture of foamed concrete was accomplished using the pre-foaming method to make a continuous pore system, which is the method of making the foam by using a foaming agent, then making the slurry by mixing the foam, water, and powders. The experiment factors are W/B, foam agent dilution ratio, and foam ratio, and test items are compressive strength, dry density, void ratio, and absorption rate, as evaluated by NRC. The experiment results showed that the sound absorption of lightweight foamed concrete satisfied NRC requirements for the absorbing materials in most of the experiments. It is thus concluded that foam ratio was the most dominant factor, and significantly affected all properties of lightweight foamed concrete in this study. W/B rarely affected total void ratio and continuous void ratio as well as compressive strength, and dry density and foam agent dilution ratio also had little effect onalmost all properties. The analysis of the correlation between NRC, absorption time, continuous void ratio, and absorption time showed that the interrelationship of the continuous void ratio was high.

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.6
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• pp.1509-1517
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• 2008

The flowers and buds of Lonicera Flower (LF), are used in Korean herbal medicine for latent-heat-clearing, antipyretic, detoxicant and anti-inflammatory ailments. This plant is used worldwide for the treatment of many types of inflammatory disease including respiratory infections, diabetes mellitus, rheumatoid arthritis and play an important role in immune reaction. These pharmaceutical effects of LF looks like to be related to its antioxidant capacity and phytochemicals containing in LF. In this study, the antioxidant activity of extract from LF was studied in vitro methods by measuring the antioxidant activity by TEAC, measuring the scavenging effects on reactive oxygen species (ROS) [superoxide anion, hydroxyl radical] and on reactive nitrogen species (RNS) [nitric oxide and peroxynitrite] as well as measuring the inhibitory effect on $Cu^{2+}$ induced human LDL oxidation and the inhibitory effect on collagen induced platelet aggregation. The LF extracts were found to have a potent scavenging activity, as well as an inhibitory effect on LDL oxidation and on platelet aggregation. In conclusion, the LF extracts have anti-oxidative and anti-atherosclerotic effects in vitro system, which can be used for developing pharmaceutical drug against oxidative stress and atherosclerosis.

• Clinical and Experimental Reproductive Medicine
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• v.28 no.3
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• pp.183-190
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• 2001

Objective: Recently, microdissection of tissue sections has been used increasingly for the isolation of morphologically identified homogeneous cell populations, thus overcoming the obstacle of tissue complexity for the analysis cell-specific expression of macromolecules. The aim of the present study was to establish the minimal conditions required for the RNA extraction and amplification from the cells captured by the laser captured microdissection. Methods : Mouse ovaries were fixed and cut into serial sections (7 im thickness). Oocytes were captured by laser captured microdissection (LCM) method by using PixCell $II^{TM}$ system. The frozen sections were fixed in 70% ethanol and stained with hematoxylin and eosin, while the paraffin sections were stained with Multiple stain. Sections were dehydrated in graded alcohols followed by xylene and air-dried for 20 min prior to LCM. All reactions were performed in ribonuclease free solutions to prevent RNA degradation. After LCM, total RNA extraction from the captured oocytes was performed using the guanidinium isothiocyanate (GITC) solution, and subsequently evaluated by reverse transcriptase-polymerase chain reaction (RT-PCR) for glyceraldehyde-3-phosphate-dehydrogenase (GAPDH). Results: With the frozen sections, detection of the GAPDH mRNA expression in the number of captured 25 oocytes were not repeatable, but the expression was always detectable from 50 oocytes. With 25 oocytes, at least 27 PCR cycles were required, whereas with 50 oocytes, 21 cycles were enough to detect GA PDH expression. Amount of the primary cDNA required for RT-PCR was reduced down to at least 0.25 $\grave{i}$ l with 50 oocytes, thus the resting 19.75 il cDNA can be used for the testing other interested gene expression. Tissue-to-slide, tissue-to-tissue forces were very high in the paraffin sections, thus the greater number of cell procurement was required than the frozen sections. Conclusion: We have described a method for analyzing gene expression at the RNA level with the homogeneously microdissected cells from the small amount of tissues with complexity. We found that LCM coupled with RT-PCR could detect housekeeping gene expression in 50 oocytes captured. This technique can be easily applied for the study of gene expression with the small amount of tissues.

• Journal of Korean Society of Environmental Engineers
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• v.27 no.1
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• pp.115-119
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• 2005

As the regulations on DBPs are tightened, many water treatment plants (WTPs) in Korea have already introduced or will introduce enhanced coagulation, alternative disinfectants and advanced treatments such as ozonization and granular activated carbon to improve drinking water qualify. After a phenol leakage accident at the Nakdong-River in 1991, 26 WIPs in Korea introduced carbon dioxide generators, but there has been no accumulation of significant operating data. This research summarizes things that should be considered for the introduction of carbon dioxide disinfection process to WTPs based on one year operation data from A WTP that has had high concentration of DBP during a specific period in the summer. The removal efficiency of DBP was $30{\sim}40%$, but those of 2-MIB, Geosmin were less than 10%. The generation rate of $ClO_2$ by-products such as chlorite and chlorate were $70{\sim}100%$ of input dosage, but the ratios increased over time. At the same time, strong chlorine odors may be produced in the distribution system when $ClO_2$ was used with $Cl_2$ as a result of reaction between the chlorite and residual chlorine.

• Applied Biological Chemistry
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• v.48 no.2
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• pp.109-114
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• 2005

A bacterium capable of emulsifying hydrocarbon, n-hexadecane, and decreasing surface tension of the culture media using oil collapsing method was isolated. The bacterium was partially identified as Bacillus sp. and named BJS-51. n-Hexadecane was the most effective carbon source for production of biosurfactant. Surface tension was decreased from 76 dyne/cm to 31 dyne/cm and CMD (critical micelle dilution) had the highest value of 5.7 at 3% n-hexadecane. Ammonium phosphate was the most effective nitrogen source, when C/N ratio was 60, surface tension and CMD were 29 dyne/cm and 9.2, respectively. Optimum pH and temperature were 7.2 and $30^{\circ}C$, respectively. Produced biosurfactant was extracted and purified using organic solvent extraction method and preparative HPLC systems. After analysis by various color reaction, this biosurfactant was identified as lipopolysaccharide. Surface tension and CMC (critical micelle concentration) of purified biosurfactant were 27 dyne/cm and 0.08 g/l, repectively. CMD was 9.2, so the yield of biosurfactant was about 0.74 g/l at the optimal conditions. The biosurfactant was very stable at wide range of $pH\;2{\sim}12$ with surface tension $29{\sim}31\;dyne/cm$ and showed $29{\sim}30\;dyne/cm$ of surface tension after heat treatment at $100^{\circ}C$ for 60 min.

• Journal of Korean Academy of Nursing Administration
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• v.10 no.2
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• pp.243-254
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• 2004

Purpose: The purpose of this study was cross-mapping unique nursing statements which were identified in the nursing records of patients with six most common cancers in Korea with the standardized nursing languages of NANDA, NIC, NOC and ICNP. Method: The subjects were 72 nursing records which covered 1,502 admission days from August 1, 2003 to June 30, 2003. They were the records of the patients of six most common cancers who were treated at the six 3rd level general hospitals in Busan and Daegu. The unique nursing statements were identified by dividing the statements from the nursing records into the single statements according to their meanings. For cross-mapping, identified unique nursing statements were classified as 'Data(D)' for the subjective, objective data of the patients and the other data such as treatment, admission, discharge, and residence of patient, 'Problem(P)' for nursing problem or diagnosis defined by the nurse's decision, 'Intervention(I)' for nursing intervention for problem solving, and 'Outcome(O)' for patient reaction and results of the provided nursing interventions. Unique nursing statements classified to D, P, I, O were cross-napped by using Microsoft Excel 2000. The statements of D were cross-mapped with ICNP Nursing phenomena, P with NANDA nursing diagnosis and ICNP nursing phenomena, I with NIC and ICNP nursing intervention, and O with NOC and ICNP nursing phenomena Result: The results of this study were as follows. 1. Number of unique nursing statements were 506 in the records of lung cancer patients (18.12%), 480 in stomach cancer(17.19%), 458 in liver cancer(16.40%), 456 in colon cancer (16.33), 457 in breast cancer (16.36%) and 436 in cervix cancer (15.60%). 2. The range of percentage of cross-mapped unique nursing statements with the standardized nursing languages were as follows: P with NANDA nursing diagnosis $87.50{\sim}100%$, I with NIC $59.72{\sim}74.43$, O with NOC $61.05{\sim}72.64%$, and D, P, I and O with ICNP $60.92{\sim}69.95%$. 3. Number of the standardized nursing languages identified in this study were 21(12.66%) from 155 NANDA nursing diagnosis, 76(15.64%) from 486 NIC Nursing interventions, 54(17.47%) from 260 NOC nursing outcomes, and 343(13.03%) from ICNP 2,634. Conclusions: By the results of this study, NANDA, NIC, NOC and ICNP were found that they can be used as the language systems for nursing record and nursing information system for cancer patients. But, further study on the unique nursing statements which were not cross-mapped with the standardized nursing language systems will be necessary.