• Journal of Radiation Industry
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• v.4 no.3
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• pp.247-252
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• 2010

The present study has been performed to select the osmotic tolerant lines using polyethylene glycol (PEG 6000) through an in vitro and in vivo mutagensis with a gamma-ray. During the screening, we selected three mutant lines that seemed to confer elevated osmotic tolerance in high concentrations of PEG 6000. Fruits of these mutants (Os-HK101, Os-HK102 and Os-HK103) were increased to sugar concentration, L-glutamine acid, vitamin C content and lycopine content than those of the wild type. Also the chlorophyll contents were few decreased more in the three mutant lines than the WT plants. Our results suggest that the Os-HK101 is characterized as osmotic stress tolerance considering the sugar concentration and lycopine content. It is expected that the result of this study can be used for breeding more competitive species with respect to contents in sugar or functional chemicals from the selected osmotic resistant lines.

• BMB Reports
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• v.55 no.4
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• pp.198-203
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• 2022

As negative regulators of cytokine signaling pathways, suppressors of cytokine signaling (SOCS) proteins have been reported to possess both pro-tumor and anti-tumor functions. Our recent studies have demonstrated suppressive effects of SOCS1 on epithelial to mesenchymal signaling in colorectal cancer cells in response to fractionated ionizing radiation or oxidative stress. The objective of the present study was to determine the radiosensitizing action of SOCS1 as an anti-tumor mechanism in colorectal cancer cell model. In HCT116 cells exposed to ionizing radiation, SOCS1 over-expression shifted cell cycle arrest from G2/M to G1 and promoted radiation-induced apoptosis in a p53-dependent manner with down-regulation of cyclin B and up-regulation of p21. On the other hand, SOCS1 knock-down resulted in a reduced apoptosis with a decrease in G1 arrest. The regulatory action of SOCS1 on the radiation response was mediated by inhibition of radiation-induced Jak3/STAT3 and Erk activities, thereby blocking G1 to S transition. Radiation-induced early ROS signal was responsible for the activation of Jak3/Erk/STAT3 that led to cell survival response. Our data collectively indicate that SOCS1 can promote radiosensitivity of colorectal cancer cells by counteracting ROS-mediated survival signal, thereby blocking cell cycle progression from G1 to S. The resulting increase in G1 arrest with p53 activation then contributes to the promotion of apoptotic response upon radiation. Thus, induction of SOCS1 expression may increase therapeutic efficacy of radiation in tumors with low SOCS1 levels.

• Molecules and Cells
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• v.28 no.6
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• pp.583-588
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• 2009

The peroxiredoxin family of peroxidase has six mammalian members (Prx 1-6). Considering their frequent up-regulation in cancer cells, Prxs may contribute to cancer cells' survival in face of oxidative stress. Here, we show that Prx 6 promotes the invasiveness of lung cancer cells, accompanied by an increase in the activity of phosphoinositide 3-kinase (PI3K), the phosphorylation of p38 kinase and Akt, and the protein levels of uPA. Functional studies reveal that these components support Prx 6-induced invasion in the sequence p38 kinase/PI3K, Akt, and uPA. The findings provide a new understanding of the action of Prx 6 in cancer.

• Proceedings of the Korean Nuclear Society Conference
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• 2002.10a
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• pp.388-388
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• 2002

• Proceedings of the Korean Nuclear Society Conference
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• 2005.10a
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• pp.727-728
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• 2005

• Korean Journal of Environmental Agriculture
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• v.39 no.3
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• pp.273-279
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• 2020

BACKGROUND: After transplanting, the recent abnormal low temperature caused physiological disorders of pepper seedlings. This study was conducted to evaluate the effects of UV-A LED, a physical elicitor, on the chilling tolerance of pepper seedlings. METHODS AND RESULTS: Seedlings were continuously irradiated with 370 and 385 nm UV-A LEDs with 30 W·m^-2 for 6 d. After that, seedlings were exposed to 4℃ for 6 h and then recovered under the normal growing condition for 2 d. There were no significant differences in growth characteristics of UV-A treatments compared to the control. Fv/Fm values of two UV-A treatments were below 0.8. Electrolyte leakage in the control was increased by chilling stress, while 385 nm UV-A had the significantly lowest value. Total phenolic content and antioxidant capacity of two UV-A treatments significantly increased due to UV-A radiation. However, total phenolic content and antioxidant capacity of the control increased due to chilling stress and tended to decrease again during the recovery time. CONCLUSION: We confirmed that UV-A light was effective to induce the chilling tolerance of pepper seedling, and the supplemental radiation of 385 nm UV-A LED before transplanting could be used as a cultivation technique to produce high quality pepper seedlings.

• Journal of Korean Biological Nursing Science
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• v.21 no.4
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• pp.308-317
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• 2019

Purpose: This study aimed to investigate the level and related factors related to health-promoting lifestyle in operating room nurses in Korea. Method: A cross-sectional descriptive survey design was employed. The data were collected using questionnaire for three weeks in December 2018 from 110 operating room nurses working for more than six months in a general hospital located in Seoul. The Health Promoting Lifestyle Profile-II, The Korean Version of the Practice Environment Scale of Nursing Work Index, and Operating nurse's Job stress Factor Scale were used. Results: Significant relationships were observed between health promoting lifestyle and educational level(p= .025), perceived economic status (p= .001) wearing a lead apron for protection from radiation during the operation(p< .001), work satisfaction(p= .016), and fatigue related to work(p= .006). Also significant correlations were identified between nursing work environment and health promoting lifestyle. However, the health promoting lifestyle was not statistically different based on occupational stress(p= .365). In multiple linear regression analyses, the level of health promoting lifestyle found to be higher in subjects who did not wear a lead apron for protection from radiation(p= .017), and who had more positive perception of the nursing work environment(p= .034). Conclusion: In order to increase health promoting lifestyle of operating nurses, the strategies to improve the nursing work environment are essential.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.4 no.2
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• pp.72-82
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• 1992

To accurately estimate nearshore current in shallow water regions. it is necessary to investigate the irregular wave transformation characteristics and radiation stress produced by random sea waves. This research is to investigate the application or the individual wave Analysis Method. the Component Wave Analysis Method and Representative Wave Analysis Method in the shallow water region. These methods were estimated by wave shallowing transformation when the waves propagate from deep water to shallow water region b)r generating regular waves, two component waves and irregular waves (Bretschneider-Mitsuyasu type). That is, the Indivisual Wave Analysis Method is to investigate from the viewpoint of shallow water transformation of wave statistical characteristics and their zero-down-crossing waves (wave height period and wave celerity). And the component Wave Analysis Method is to investigate from the view point of shallow water transformation of basic frequency component wave and their interference frequency component wave. In addition, this research is to compare the measured mean water level elevation with the calculated one from radiation stress of irreguar waves that is assumed in the three methods above.

• Korean Journal of Adult Nursing
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• v.13 no.4
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• pp.560-570
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• 2001

Purpose: The purpose of this study is to identify factors of the causal attribution of cancer and to determine related variables. Method: Subjects were one hundred and thirty three cancer patients. The tool of the perceived causal attribution used was developed by authors and basically founded on Kim's work(1993). The SAS program was used to analyze the data along with descriptive statistics, t-test, ANOVA, Duncan's Multiple range test, and Principal component analysis and varimax rotation. Results: 1) The perceived causal attribution measurement revealed four factors; overload, destiny, stress, and constitution. The total percentage of variance explained by the four factors was 44.3%. 2) The scores of destiny on women, having religion, unemployed, lower level of education, no spouse, groups of uterine cervix and lung cancer, not receiving an operation and receiving radiation were significantly higher than those other groups. 3) The scores of stress on women, having religion, and not having a job were significantly higher than those on men, without religion, and having a job. 4) The scores of constitution on those in their forties, women, not receiving an operation and receiving radiation were significantly higher than for those in their sixties, men, receiving operation and not receiving radiation. There was no significant difference in the factor scores of overload by any variables. Conclusion: Factors of the perceived causal attribution of cancer among Korean cancer patients were overload, destiny, stress, and constitution. The scores of each factor the perceived causal attribution was significantly different by general and disease related characteristics.

• Journal of Microbiology and Biotechnology
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• v.21 no.6
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• pp.574-581
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• 2011

A Gram-positive bacterium was isolated from the saline soils of Jangpura (U.P.), India, and showed high-level of radiation-resistant property and survived upto 12.5 kGy dose of gamma radiation. The 16S rDNA sequence of this strain was examined, identified as Bacillus sp. strain HKG 112, and was submitted to the NCBI GenBank (Accession No. GQ925432). The mechanism of radiation resistance and gene level expression were examined by proteomic analysis of whole-cell extract. Two proteins, 38 kDa and 86.5 kDa excised from SDS-PAGE, which showed more significant changes after radiation exposure, were identified by MALDI-TOF as being flagellin and S-layer protein, respectively. Twenty selected 2-DE protein spots from the crude extracts of Bacillus sp. HKG 112, excised from 2- DE, were identified by liquid chromatography mass spectrometry (LC-MS) out of which 16 spots showed significant changes after radiation exposure and might be responsible for the radiation resistance property. Our results suggest that the different responses of some genes under radiation for the expression of radiation-dependent proteins could contribute to a physiological advantage and would be a significant initial step towards a fullsystem understanding of the radiation stress protection mechanisms of bacteria in different environments.