• Korean Journal of Veterinary Research
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• v.55 no.2
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• pp.149-152
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• 2015

An abnormally enlarged right ovary and a mass in fat surrounding the right kidney were discovered in a dairy cow during routine postmortem examination at slaughter. The ovary was dark reddish and multinodular in shape. Numerous cystic structures were identified in the mass. Histopathologically, the ovary was completely replaced with large, uniform, polyhedral neoplastic cells containing vesicular nuclei and prominent nucleoli. The mitotic index was high. In the lymphatic vessels, tumor emboli were observed. Another mass in the fat surranding the right kidney had the same histological features as the ovarian mass. This animal was diagnosed with malignant dysgerminoma and metastasis to other peritoneal organs.

• BMB Reports
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• v.34 no.5
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• pp.457-462
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• 2001

Using the monomolecular film technique, we compared the interfacial properties of Staphylococcus simulans lipase (SSL) and Staphylococcus aureus lipase (SAL). These two enzymes act specifically on glycerides without any detectable phospholipase activity when using various phospholipids. Our results show that the maximum rate of racemic dicaprin (rac-dicaprin) hydrolysis was displayed at pH 8.5, or 6.5 with Staphylococcus simulans lipase or Staphylococcus aureus lipase, respectively The two enzymes interact strongly with egg-phosphatidyl choline (egg-PC) monomolecular films, evidenced by a critical surface pressure value of around $23\;mN{\cdot}m^{-1}$. In contrast to pancreatic lipases, $\beta$-lactoglobulin, a tensioactive protein, failed to inhibit Staphylococcus simulans lipase and Staphylococcus aureus lipase. A kinetic study on the surface pressure dependency, stereoselectivity, and regioselectivity of Staphylococcus simulans lipase and Staphylococcus aureus lipase was performed using optically pure stereoisomers of diglycerides (1,2-sn-dicaprin and 2,3-sn-dicaprin) and a prochiral isomer (1,3-sn-dicaprin) that were spread as monomolecular films at the air-water interface. Both staphylococcal lipases acted preferentially on distal carboxylic ester groups of the diglyceride isomer (1,3-sn-dicaprin). Furthermore, Staphylococcus simulans lipase was found to be markedly stereoselective for the sn-3 position of the 2,3-sn-dicaprin isomer.

• BMB Reports
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• v.49 no.9
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• pp.457-458
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• 2016

Recent studies have strongly implicated postsynaptic scaffolding proteins such as SAPAP3 or Shank3 in the pathogenesis of various mood disorders, including autism spectrum disorder, bipolar disorder (BD), and obsessive-compulsive disorders. Neural Abelson-related gene-binding protein 2 (nArgBP2) was originally identified as a protein that interacts with SAPAP3 and Shank3. Recent study shows that the genetic deletion of nArgBP2 in mice leads to manic/bipolar-like behavior resembling symptoms of BD. However, the function of nArgBP2 at synapse, or its connection with the synaptic dysfunctions, is completely unknown. This study provides compelling evidence that nArgBP2 regulates the spine morphogenesis through the activation of Rac1/WAVE/PAK/cofilin pathway, and that its ablation causes a robust and selective inhibition of excitatory synapse formation, by controlling actin dynamics. Our results revealed the underlying mechanism for the synaptic dysfunction caused by nArgBP2 downregulation that associates with analogous human BD. Moreover, since nArgBP2 interacts with key proteins involved in various neuropsychiatric disorders, our finding implies that nArgBP2 could function as a hub linking various etiological factors of different mood disorders.

• Korean Journal of Medicinal Crop Science
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• v.17 no.3
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• pp.161-164
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• 2009

This study was conducted to investigate the weight of crown part effect on growth and bolting response of Ostericum koreanum Kitagawa. Bolting rate with crown part weight was represented 0%, 0%, 4.1%, 12.9%, 25.1%, 34.9% corresponding to each crown part weight 5 g, 10 g, 20 g, 30 g, 40 g, 50 g. The heavier the crown part weight was the higher the direction rate rose and the flowering time came earlier. The dried root amount was the highest at 30 g and crown part number, the length of root, and crown diameter showed all the highest figures at 30 g. The relation between amount per 10 g and outcrop weight increased on secondary regression formula as weight increased and reached its peak at 29.7 g and then decreased over 29.7 g.

• Restorative Dentistry and Endodontics
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• v.8 no.1
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• pp.61-68
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• 1982

This study was undertaken to compare the histopathological effects of the fractionated doses of 2,400, 4,800 and 7,200 rads of Cobalt-60 irradiation on the teeth of the sixty eight rats (Wester strain), weighing approximately 130gm. They were devided into four groups; no irradiation as a control (Group I) and others (Group II, III, and IV) which received different doses of radiation using 1.25 Mev RAC-120 Cobalt-60 Teletherapy Unit with exposure dose rate 69 rads/min, TSD 80cm. Experimental animals received 600 rads every three days until the total dosage of their groups were reached. Experimental animals were sacrified at the 1st day, 1st, 2nd, 3rd, 4th, 5th and 6th week irradiation. The following histopathologic findings were obtained: 1. The Group II, received 2,400 rads, showed no histopamologic changes essentially same as those of the control Group. 2. In the Groups III and IV, received 4,800 rads and 7,200 rads, disarray of the normal palasading arrangement of the odontoblastic cells, vacuolar degeneration in the odontoblastic layer, osteodentin and dentinal niches in the anterior teeth were observed. 3. There were no distinct changes in the posterior teeth in the Groups III and IV. 4. The ameloblast cells seemed to be less sensitive to radiation damage than the odontoblast cells.

• 유체기계공업학회:학술대회논문집
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• 2004.12a
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• pp.697-702
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• 2004

LG Electronics developed linear compressors for a household refrigerator which can reduce energy consumption up to $24\%$ by only Drop-ln instead of a conventional reciprocating compressor. The linear compressor is composed of a linear oscillating motor, a piston directly coupled with the linear motor, a cylinder and the specific discharge system of LG. Because of this free-piston structure, the position of the piston can be changeable according to the voltage variation, load variation and so on. By this reason, LG linear compressor has been in need of an additional unit to control the position of the free-piston stably. In this paper we will introduce the innovative technology which is able to operate the linear compressor without controlling the piston position like a conventional reciprocating compressor.

• Bulletin of the Korean Chemical Society
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• v.34 no.10
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• pp.2973-2977
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• 2013

In this study, we developed a liquid crystal (LC)-based optical sensor for monitoring enzymatic activity through orientational changes in liquid crystals (LCs) coupled to the properties of a poly-${\small{L}}$-lysine (PLL)-based polymeric membrane. We prepared a PLL-based polymeric membrane at the planar interface between the thermotropic liquid crystal and aqueous phases. The PLL-based polymeric membrane was obtained by contacting the PLL solution with water immiscible LCs, 4-cyano-4'-pentyl-biphenyl (5CB) doped with adipoyl chloride. We then investigated the membrane properties by examining the permeability of the membrane to phospholipids, 1,2-didodecanoyl-rac-glycero-3-phosphocholine (DLPC). The permeability of the membrane to transport phospholipids was monitored through the orientational transition of 5CB in contact with the dispersions of DLPC. Since trypsin can enzymatically catalyze the hydrolysis of PLL, we incubated an aqueous trypsin solution with the membrane for 2 h at room temperature to cause an increase in the permeability of the polymeric membrane to DLPC. As a result, a bright to dark optical shift of LCs was observed, which implied that an enzymatic reaction between trypsin and PLL-based membrane occurred. Two control experiments using chymotrypsin and bovine serum albumin (BSA) revealed no sign of improved permeability based on the orientational transition of LCs.

• Molecules and Cells
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• v.20 no.2
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• pp.280-287
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• 2005

The insulin-mediated Ras/mitogen-activated protein (MAP) kinase cascade was examined in SK-N-BE(2) and PC12 cells, which can and cannot produce reactive oxygen species (ROS), respectively. Tyrosine phosphorylation of the insulin receptor and insulin receptor substrate 1 (IRS-1) was much lower in SK-N-BE(2) cells than in PC12 cells when the cells were treated with insulin. The insulin-mediated interaction of IRS-1 with Grb2 was observed in PC12 but not in SK-N-BE(2) cells. Moreover, the activity of extracellular-signal-related kinase (ERK) was much lower in SK-N-BE(2) than in PC12 cells when the cells were treated with insulin. Application of exogenous $H_2O_2$ caused increased tyrosine phosphorylation and Grb2 binding to IRS-1 in SK-N-BE(2) cells, while exposure to an $H_2O_2$ scavenger (N-acetylcysteine) or to a phophatidylinositol-3 kinase inhibitor (wortmannin), and expression of a dominant negative Rac1, decreased the activation of ERK in insulin-stimulated PC12 cells. These results indicate that the transient increase of ROS is needed to activate ERK in insulin-mediated signaling and that an inability to generate ROS is the reason for the insulin insensitivity of SK-N-BE(2) cells.

• Genomics & Informatics
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• v.12 no.4
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• pp.247-253
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• 2014

Osteosarcoma is the most common primary bone tumor, generally affecting young people. While the etiology of osteosarcoma has been largely unknown, recent studies have suggested that cyclooxygenase-2 (COX-2) plays a critical role in the proliferation, migration, and invasion of osteosarcoma cells. To understand the mechanism of action of COX-2 in the pathogenesis of osteosarcoma, we compared gene expression patterns between three stable COX-2-overexpressing cell lines and three control cell lines derived from U2OS human osteosarcoma cells. The data showed that 56 genes were upregulated, whereas 20 genes were downregulated, in COX-2-overexpressed cell lines, with an average fold-change > 1.5. Among the upregulated genes, COL1A1, COL5A2, FBN1, HOXD10, RUNX2, and TRAPPC2 are involved in bone and skeletal system development, while DDR2, RAC2, RUNX2, and TSPAN31 are involved in the positive regulation of cell proliferation. Among the downregulated genes, HIST1H1D, HIST1H2AI, HIST1H3H, and HIST1H4C are involved in nucleosome assembly and DNA packaging. These results may provide useful information to elucidate the molecular mechanism of the COX-2-mediated malignant phenotype in osteosarcoma.

• Journal of the Korean Magnetics Society
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• v.22 no.2
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• pp.45-48
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• 2012

Feed-back type 3-axis flux-gate magnetometer using Co-based amorphous ribbon (Metglass$^{(R)}$2714A) was constructed in this work. Measuring range of magnetic field and frequency were ${\pm}100\;{\mu}T$ and dc~10 Hz respectively. For the interface to computer, microcontroller and 24 bit ADC (Analog to Digital Converter) were employed and resolution of digital output was 0.1 nT. Magnetometer noise of analog output was 5 pT/$\sqrt{Hz}$ at 1 Hz. Digital output of the magnetometer showed linearity of $1{\times}10^{-4}$ and the offset drift was smaller than 0.2 nT during 1 h.