BMB Reports
- Volume 34 Issue 5
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- Pages.457-462
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- 2001
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- 1976-670X(eISSN)
Comparative Kinetic Studies of Two Staphylococcal Lipases Using the Monomolecular Film Technique
- Sayari, Adel (Unite de Lipolyse Enzymatique, ENIS BPW) ;
- Verger, Robert (Laboratoire de Lipolyse Enzymatique, UPR 9025 du CNRS) ;
- Gargouri, Youssef (Unite de Lipolyse Enzymatique, ENIS BPW)
- Received : 2001.05.08
- Accepted : 2001.07.31
- Published : 2001.09.30
Abstract
Using the monomolecular film technique, we compared the interfacial properties of Staphylococcus simulans lipase (SSL) and Staphylococcus aureus lipase (SAL). These two enzymes act specifically on glycerides without any detectable phospholipase activity when using various phospholipids. Our results show that the maximum rate of racemic dicaprin (rac-dicaprin) hydrolysis was displayed at pH 8.5, or 6.5 with Staphylococcus simulans lipase or Staphylococcus aureus lipase, respectively The two enzymes interact strongly with egg-phosphatidyl choline (egg-PC) monomolecular films, evidenced by a critical surface pressure value of around
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