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Evaluation of Fertilizing Ability using Frozen Thawed Sperm in the Longtooth Grouper, Epinephelus bruneus

  • Oh, Seong-Rip;Lee, Chi-Hoon;Kang, Hyeong-Cheol;Song, Young-Bo;Kim, Hyung-Bae;Lee, Young-Don
    • Development and Reproduction
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    • v.17 no.4
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    • pp.345-351
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    • 2013
  • This study examines the effects on fertilization rate (FR), hatching rate (HR), and normal individual rate after artificial fertilization using frozen thawed sperm according to the cryoprotectant (DMSO) concentration and the period of cryopreserved sperm of longtooth grouper, Epinephelus bruneus. Performing artificial fertilization using frozen-thawed sperm, after freezing the sperm at different DMSO concentration of 5.0%, 7.5%, 10.0% respectively, FR were (DMSO 5.0%: $99.5{\pm}0.8%$, DMSO 7.5%: $99.5{\pm}0.7%$, and DMSO 10.0%: $99.6{\pm}0.6%$). The results are not significantly different from the control fresh sperm (100%). HR also (DMSO 5.0%: $96.2{\pm}2.3%$, DMSO 7.5%: $95.3{\pm}3.6%$, 10.0%: $96.6{\pm}1.8%$) were not significantly different in each group. The normal individual rate after hatching using with control fresh sperm ($98.4%{\pm}0.5$) and DMSO concentration level of 5.0% ($97.8{\pm}0.1%$) were not significantly different. However, with 7.5% ($97.2{\pm}0.6%$) and 10.0% DMSO concentrations ($95.9{\pm}0.2%$) are lower than the normal individual rate after hatching observed in the control and 5.0% DMSO. Performing artificial fertilization using frozen-thawed sperm at different frozen period (2 days, 2 years, and 3 years), 10% DMSO FR and HR of 3 years (FR; $66.8{\pm}1.8%$, HR: $82.0{\pm}12.9%$) and 2 years (FR; $78.5{\pm}14.8%$, HR: $79.3{\pm}0.6%$) cryopreserved sperm were lower than control (FR; 100%, HR: $91.1{\pm}3.6%$) and 2 days cryopreserved sperm (FR; $99.6{\pm}0.6%$, HR: $96.6{\pm}1.8%$). These results suggest suitable DMSO concentration ranges of cryopreservation sperm for E. bruneus is 5 to 10% and with 2 to 3 years cryopreservation period, cryopreservation sperm can be useful for seed production.

Classification of Fishing Gear (어구의 분류)

  • 김대안
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.32 no.1
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    • pp.33-41
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    • 1996
  • In order to obtain the most favourable classification system for fishing gears, the problems in the existing systems were investigated and a new system in which the fishing method was adopted as the criterion of classification and the kinds of fishing gears were obtained by exchanging the word method into gear in the fishing methods classified newly for eliminating the problems was established. The new system to which the actual gears are arranged is as follows ; (1)Harvesting gear \circled1Plucking gears : Clamp, Tong, Wrench, etc. \circled2Sweeping gears : Push net, Coral sweep net, etc. \circled3Dredging gears : Hand dredge net, Boat dredge net, etc. (2)Sticking gears \circled1Shot sticking gears : Spear, Sharp plummet, Harpoon, etc. \circled2Pulled sticking gears : Gaff, Comb, Rake, Hook harrow, Jerking hook, etc. \circled3Left sticking gears : Rip - hook set line. (3)Angling gears \circled1Jerky angling gears (a)Single - jerky angling gears : Hand line, Pole line, etc. (b)Multiple - jerky angling gears : squid hook. \circled2Idly angling gears (a)Set angling gears : Set long line. (b)Drifted angling gears : Drift long line, Drift vertical line, etc. \circled3Dragged angling gears : Troll line. (4)Shelter gears : Eel tube, Webfoot - octopus pot, Octopus pot, etc. (5)Attracting gears : Fishing basket. (6)Cutoff gears : Wall, Screen net, Window net, etc. (7)Guiding gears \circled1Horizontally guiding gears : Triangular set net, Elliptic set net, Rectangular set net, Fish weir, etc. \circled2Vertically guiding gears : Pound net. \circled3Deeply guiding gears : Funnel net. (8)Receiving gears \circled1Jumping - fish receiving gears : Fish - receiving scoop net, Fish - receiving raft, etc. \circled2Drifting - fish receiving gears (a)Set drifting - fish receiving gears : Bamboo screen, Pillar stow net, Long stow net, etc. (b)Movable drifting - fish receiving gears : Stow net. (9)Bagging gears \circled1Drag - bagging gears (a)Bottom - drag bagging gears : Bottom otter trawl, Bottom beam trawl, Bottom pair trawl, etc. (b)Midwater - drag gagging gears : Midwater otter trawl, Midwater pair trawl, etc. (c)Surface - drag gagging gears : Anchovy drag net. \circled2Seine - bagging gears (a)Beach - seine bagging gears : Skimming scoop net, Beach seine, etc. (b)Boat - seine bagging gears : Boat seine, Danish seine, etc. \circled3Drive - bagging gears : Drive - in dustpan net, Inner drive - in net, etc. (10)Surrounding gears \circled1Incomplete surrounding gears : Lampara net, Ring net, etc. \circled2Complete surrounding gears : Purse seine, Round haul net, etc. (11)Covering gears \circled1Drop - type covering gears : Wooden cover, Lantern net, etc. \circled2Spread - type covering gears : Cast net. (12)Lifting gears \circled1Wait - lifting gears : Scoop net, Scrape net, etc. \circled2Gatherable lifting gears : Saury lift net, Anchovy lift net, etc. (13)Adherent gears \circled1Gilling gears (a)Set gilling gears : Bottom gill net, Floating gill net. (b)Drifted gilling gears : Drift gill net. (c)Encircled gilling gears : Encircled gill net. (d)Seine - gilling gears : Seining gill net. (e)Dragged gilling gears : Dragged gill net. \circled2Tangling gears (a)Set tangling gears : Double trammel net, Triple trammel net, etc. (b)Encircled tangling gears : Encircled tangle net. (c)Dragged tangling gears : Dragged tangle net. \circled3Restrainting gears (a)Drifted restrainting gears : Pocket net(Gen - type net). (b)Dragged restrainting gears : Dragged pocket net. (14)Sucking gears : Fish pumps.

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Seasonal and Regional Occurrence of Oriental Beetle (Blitopertha orientalis) in Korean Golf Courses (골프장에서 등얼룩풍뎅이(Blitopertha orientalis)의 계절 및 지리적 발생)

  • Lee, Dong-Woon;Kim, Jae-Ho;Shin, Jong-Chang;Yeom, Ju-Rip;Jeon, Jae-Chan;Shin, Hong-Kun;Choo, Ho-Yul
    • Asian Journal of Turfgrass Science
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    • v.22 no.1
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    • pp.35-48
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    • 2008
  • Seasonal and regional occurrence of oriental beetle, Blitopertha (=Exomala) orientalis was investigated using pheromone traps and soil sampling at three golf clubs in Busan, Gunpo, and Gimpo from 2004 to 2007. Adults of Blitopertha orientalis were found from early June to late August, but peak times were different depending on the location and year. Peak day of adult occurrence of B. orientalis was 25 June,2006 and 22 June 2007 in Busan, but 30 June,2006 and 29 June,2007 in Gunpo. Mean numbers of B. orientalis adults attracted to pheromone trap varied with locations. Larval development was faster in Busan than in Gunpo. The 2nd instars of B. orientalis was found on 27 August, 2004 in Gunpo whereas 3rd instars were found in Busan in the same period. In Busan, overwintered 3rd instars pupated from early May to mid-June and emerged from early June. Eggs laid at this time were hatched from late June Most 3rd instars were found at middle August and started to overwinter. The peak time of mating was 20:00 to 22:00 hour of the day.

Application of Incidence Angle on Lumbar Spine Anteroposterior General Radiography Image according to Measured Intervertebral Disc Angle (방사선 일반 정면검사에서 허리뼈 추간판 계측 값에 따른 입사각 적용)

  • Moon, Seul-Ji-A;Kim, Gyeong-Rip;Cho, Hee-Jung;Sung, Soon-Ki;Kwak, Jong-Hyeok
    • The Journal of the Korea Contents Association
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    • v.19 no.6
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    • pp.471-480
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    • 2019
  • L-spine 3~4, L-spine 4~5, and L-spine 5~Sacrum 1 intervertebral disc(IVD) angle according to gender, age, body mass index(BMI), lumbar lordosis angle(LLA) were compared and analyzed. The anteriorposterior incidence angle of L-spine 3 ~ 4, L-spine 4 ~ 5 and L-spine 5~Sacrum 1 in body mass index were 5.66, 13.23 and 29.13 degrees in the head direction and L-spine 3 4, L-spine 4 ~ 5, L-spine 5~Sacrum 1 had 6.32 degrees, 16.09 degrees and 35.36 degrees in the head direction. The distortion area ratio comparison was performed with the phantom image using the proposed incidence angle. There was a significant difference in L-spine 4~5 and L-spine 5~Sacrum 1 IVD angle relative to body mass index and LLA(p<0.05), IVD angle and LLA were positively correlated(p<0.05).As a result of evaluating the usefulness of the image by applying the incidence angles of the disc angles according to the phantom angle of deviation to the head direction as 11 degrees for L4 and 26 degrees for L5, the distortion ratio area decreased from 14.90% to 12.11% in L4, And from 15.25% to 13.72% in L5. In anteriorposterior image of the Lumbar spine applying the incidence angle according to the measured disc angle, it is possible to reduce the distortion to purpose L4, L5. And improved the quality and diagnostic information of the target site.

Categorical Research On the Past Chiefs of Presidential Security Service (역대 대통령경호실장 유형분석)

  • Cho, Kwang-Rae
    • Korean Security Journal
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    • no.33
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    • pp.229-260
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    • 2012
  • The purpose of this study is to categorize the past Chiefs of Presidenteal Security Service so far based on their level of expertise and authority so that we can identify some specific reasons as to why some of them are recognized as successful while others are not, and that the categorization will be a guideline to future successful administration. In order to do so, a quantitative research has been implemented through meetings of a group security science expert who has been in security business for substantial amount of time. The result is as following: Authoritative/professional: 2nd Chief of P.S.S. Park, Jong-Gyu, 5th Chief of P.S.S. Jang, Se-Dong, 7th Chief of P.S.S. Lee, Hyeon-Wu, 8th Chief of P.S.S. Choi, Seok-Rip. Authoritative/Non-professional: 1st Chief of P.S.S. Hong, Jong-cheol, 3rd Chief of P.S.S. Cha, Ji-cheol, 6th Chief of P.S.S. Ahn, Hyeon-Tae, 14th Chief of P.S.S. Kim, In-Jong. Non-authoritative/professional: 4th Chief of P.S.S. Jeong, Dong-Ho, 9th Chief of P.S.S. Park, Sang-Beom, 13th Chief of P.S.S. Yom. Sang-Guk. Non-authoritative/Non-professional: 10th Chief of P.S.S. Kim, Gwang-Seok, 11th Chief of P.S.S. Ahn, Ju-Seop, 12th Chief of P.S.S. Kim, Se-Ok. This categorization research on the Chief of P.S.S. suggests that any president, to be a successful one, must choose the Chief of P.S.S. based on his/her expertise in order to secure national security.

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Synthesis of Resin Derivatives and Purification of Protein - Synthesis of p-Aminoanilinylsuccinyl-AH-Sepharose 4B and Purification of Protein in Pleurotus cornucopiae - (친화성 고분자 유도체의 합성 및 단백질의 분리정제에 관한 연구 - p-Aminoanilinylsuccinyl-AH-Sepharose 4B의 합성 및 흰느타리버섯 중 단백질의 정제 -)

  • Min, Tae-Jin;Kim, Yong-Rip;Park, Sang-Shin;Lee, Soo-Yong
    • The Korean Journal of Mycology
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    • v.17 no.3
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    • pp.105-113
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    • 1989
  • For selective purification of proteins in Pleurotus cornucopiae, affinity chromatography was performed by p-aminoanilinylsuccinyl-AH-Sepharose 4B gel synthesized by treating p-phenylene diamine with succinyl-AH-Sepharose 4B, which was prepared by treating AH-Sepharose 4B with succinic anhydride. The capacity of p-aminoanilinyl ligand group was 6.1 micromole per milliliter of gel. Total apparent molecular weight of the affinity proteins eluted from the synthesized gel was 167 KD, which were a protein complex of 130 KD and 37 KD. The contents of the nonpolar, polar, positively and/or negatively charged amino acids in the affinity protein were 44.57%, 24.75%, 21.25%, and 9.43%, respectively. Total apparent molecular weight of the affinity proteins eluted from the AH-Sepharose 4B gel was 95.2 KD, which were a protein complex of 61 KD, 31 KD and 3.2 KD. The contents of the nonpolar, positively and/or negatively charged amino acids in the affinity protein by AH-Sepharose 4B gel were 44.05%, 29.13%, and 12.91% respectively.

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A Phylogenetic Relationship between Foreign and Korean Strains of Flammulina velutipes Identified by rDNA-ITS Sequence Analysis (Flammulina velutipe의 국내 균주와 외래 균주 간의 ITS region을 이용한 계통학적 유연관계 분석)

  • Hwang, Gwang-Rip;Woo, Ju-Ri;Yoon, Hyeok-Jun;Lee, Chang-Yun;Lee, Sang-Han;Kong, Won-Sik;Kim, Jong-Guk
    • Journal of Life Science
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    • v.22 no.1
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    • pp.62-73
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    • 2012
  • This study was carried out to investigate the genetic relationship of Flammulina velutipes with other species. The ribosomal DNA cluster containing 4 rRNA genes from F. velutipes 4154 were sequenced. The length of the rDNA cluster sequence was estimated at 7,403 bp long and consisted of 1,806 bp of SSU rDNA, 245 bp of ITS 1 region, 159 bp of 5.8S rDNA, 308 bp of ITS 2 region, 3,402 bp of LSU rDNA, 1,400 bp of IGS 1 region, and 83 bp of 5S rDNA. The F. velutipes 4154 genes were contained in the rDNA cluster of F. velutipes in the order of SSU rDNA - ITS 1 - 5.8S rDNA - ITS 2 - LSU rDNA - IGS 1 - 5S rDNA. The phylogenetic relationships of 20 strains of Tricholomataceae and Physalacriaceae were analyzed by conducting distance analysis using the Neighbor-joining (NJ) method. The 20 strains used in this study were divided into three groups and the strains of the genus Flammulina were related very closely to strains of Physalacria bambusae.

A Study on Korean Twill Fabrics in the 17th Century (17세기 한국 능(綾)직물 연구)

  • Cho, Hyo-Sook;Lee, Eunjin
    • Journal of the Korean Society of Costume
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    • v.63 no.4
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    • pp.56-69
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    • 2013
  • This study studies the fabrics from excavated 17th century tombs of Mrs. Min from Yeoheung family(1586~1656), Yeo-on Kim(1596~1665) and Won-rip Choi(1618~1690) and attempts to clarify the relationship between the fashion trend in fabrics of those times and the background behind it by viewing and examining the proportion of twill fabrics to the total silk fabrics and the characteristics of its weave and patterns. Looking into fabrics from the above tombs, twill fabrics accounted for 10.4%(13 pieces) 19.3%(16 pieces) and 9.2%(9 pieces) of total silk fabrics in each of the respective tombs. This forms a remarkable contrast with the fact that there was only one piece of twill silk fabrics(0.5%) and not any from the 16th century tombs of Mrs. Yoon from Papyeong family (0.5%) and Soo-ryoon Sim(0%). In particular, the percentage of hwamun-neung(patterned twill fabrics) in each of the tombs is 8.0%(10 pieces), 13.3%(11 pieces), 9.2%(9 pieces), which is much higher than that of non-patterned one. This is common to the twill fabrics from above three 17th century tombs. Patterns of hwamun-neung(patterned twill), simplified small flower patterns or geometrical figures, from the three excavated tombs are mostly arranged sporadically with blank space. It is supposed that these figurative characteristics reflected the aesthetic sense of the gentry at that time which valued simplicity and moderation for their Confucian standard. This phenomenon of increased use and production of twill fabrics in the 17th century resulted from different factors such as wars like Japanese Invasion of Korea(1592~1598), economic difficulty, government regulations against the production of high-class fabrics, development of weaving skill and its fixation, changes of fabric production environments, and changes of aesthetic sense preferring naive and moderate things to showy ones. As for the weaving characteristics of twill fabrics from the three 17th century tombs 3 leaf warp-faced twill was often used for the ground texture and 4 leaf warp-faced one was occasionally used. For pattern texture 6 leaf weft-faced twill was frequently used, 4 leaf weft-faced twill and 3 leaf weft-faced twill were used at times, and floating one was occasionally used as well.

Nitrogen Removal Characteristic of Excreta Wastewater Using SBR and MBR Processes (SBR 및 MBR 공정을 이용한 분뇨폐수에서의 질소제거 특성)

  • Jung, Jin-Hee;Yoon, Young-Nae;Lee, Seul-Kee;Han, Young-Rip;Lee, Seung-Chul;Choi, Young-Ik
    • Journal of Environmental Science International
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    • v.24 no.11
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    • pp.1485-1491
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    • 2015
  • There are two treatment processes that are currently applied to ships are the biological treatment process using the activated sludge and the electrochemical treatment. However, neither of them are able to remove both nitrogen and phosphorus due to their limited ability to remove organic matters, which are main causes of the red tide. This study was conducted to identify the characteristics of nitrogen removal factors from manure wastewater by replacing the final settling tank in SBR (Sequencing Batch Reactor) process and applying immersion type hollow fiber membrane. SBR process is known to have an advantage of the least land requirement in special environment such as in ship and the immersion type hollow fiber membrane is more stable in water quality change. As the result, the average in the cases of DO (Dissolved Oxygen) is 2.9(0. 6~3.9) mg/L which was determined to be the denitrifying microorganism activity in anaerobic conditions. The average in the cases of ORP (Oxidation Reduction Potential) is 98.4~237.3 mV which was determined to be the termination of nitrification since the inflection point was formed on the ORP curve due to decrease in the stirring treatment after the aeration, same as in the cases of DO. Little or no variation in the pH was determined to have positive effect on the nitrification. T-N (Total Nitrigen) removal efficiencies of the finally treated water were 71.4%, 72.3% and 66.5% in relatively average figures, thus was not a distinct prominence. In being applied in ships in the future, the operating conditions and structure improvements are deemed necessary since the MEPC (Marine Environment Protection Committee). 227(64) ship sewage nitrogen is less than the standard of 20 Qi/Qe mg/L or the removal rate of 70%.

A Simulation of Wave Induced Current Around the Jeju New Harbor (제주외항 건설에 따른 주변 해역의 해빈류 변화 예측)

  • Kwak, Moon-Su;Pyun, Chong-Kun
    • Journal of the Korean Society of Hazard Mitigation
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    • v.4 no.3 s.14
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    • pp.1-8
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    • 2004
  • In this study, the change of the current in the coastal zone before and after the construction of Jeju new harbor was predicted by using the numerical model, which uses Hardy-Cross method. The numerical model was carried out for the present state, before the construction, and the state after the construction, and for the wave direction the NNW direction for winter and NE direction for summer were tested so that the seasonal change may be considered. The computation result shows that a large amount of the wave induced current was occurred when there were high waves coming in from NNW direction before and after the construction. Also, before the construction a longshore current occurred moving from the west to the east at the new harbor construction site so that it formed a rip current in the Hwabuk-dong front sea. And also, after the construction, the tip current produced changed into nearshore circulating current and a small circulating current appeared at the harbor entrance. On the other hand, at Samyang 4each, which is 3.0km away from the new harbor in the NE direction, shows that there was a longshore current occurred from the west to the east, which is in the opposite direction the new harbor, and the effect on the new harbor by sediment transport at Samyang beach is thought to be very small.